YDR494W / RSM28

Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR494W / RSM28

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:3.25|P-value:7.78E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.25|P-value:7.74E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:4.89|P-value:9.69E-7 ID:SGTC_145|Compound:3737-0113|FD-Score:3.34|P-value:5.72E-4 ID:SGTC_264|Compound:promethazine|FD-Score:5.23|P-value:1.82E-7 ID:SGTC_310|Compound:1273-0058|FD-Score:3.61|P-value:2.16E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:-3.19|P-value:9.96E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:-4.67|P-value:2.94E-6 ID:SGTC_409|Compound:arachidonic acid|FD-Score:3.54|P-value:2.83E-4 ID:SGTC_507|Compound:nimesulide|FD-Score:3.43|P-value:4.13E-4 ID:SGTC_512|Compound:prima-1|FD-Score:3.56|P-value:2.58E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:5.73|P-value:1.25E-8 ID:SGTC_575|Compound:k072-0246|FD-Score:3.34|P-value:5.77E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:-3.53|P-value:3.12E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:4.53|P-value:5.16E-6 ID:SGTC_852|Compound:0418-0143|FD-Score:-4.58|P-value:4.39E-6 ID:SGTC_867|Compound:0518-0118|FD-Score:5.35|P-value:9.79E-8 ID:SGTC_982|Compound:1491-0518|FD-Score:-3.57|P-value:2.67E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-7.88|P-value:9.50E-15 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.22|P-value:8.52E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.27|P-value:7.22E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:4.21|P-value:2.05E-5 ID:SGTC_1588|Compound:farnesol|FD-Score:4.14|P-value:2.81E-5 ID:SGTC_1602|Compound:st001878|FD-Score:3.51|P-value:3.18E-4 ID:SGTC_1621|Compound:st003705|FD-Score:-3.29|P-value:7.09E-4 ID:SGTC_1896|Compound:st058441|FD-Score:-3.70|P-value:1.66E-4 ID:SGTC_1923|Compound:st058458|FD-Score:-3.26|P-value:7.83E-4 ID:SGTC_1933|Compound:st074711|FD-Score:6.72|P-value:3.06E-11 ID:SGTC_1962|Compound:st076595|FD-Score:4.62|P-value:3.43E-6 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:-3.59|P-value:2.44E-4 ID:SGTC_1966|Compound:st070145|FD-Score:3.95|P-value:5.94E-5 ID:SGTC_1980|Compound:st071811|FD-Score:-3.75|P-value:1.36E-4 ID:SGTC_1984|Compound:st072354|FD-Score:3.45|P-value:3.83E-4 ID:SGTC_2068|Compound:5226020|FD-Score:3.71|P-value:1.50E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.34|P-value:1.17E-5 ID:SGTC_2161|Compound:5662504|FD-Score:-3.24|P-value:8.48E-4 ID:SGTC_2163|Compound:5671233|FD-Score:-3.41|P-value:4.73E-4 ID:SGTC_2173|Compound:5763948|FD-Score:-3.20|P-value:9.43E-4 ID:SGTC_2181|Compound:6316240|FD-Score:3.18|P-value:9.75E-4 ID:SGTC_2239|Compound:6685329|FD-Score:4.01|P-value:4.65E-5 ID:SGTC_2336|Compound:9001819|FD-Score:-3.45|P-value:4.08E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.21|P-value:8.94E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:4.30|P-value:1.40E-5 ID:SGTC_2555|Compound:cholic acid|FD-Score:3.70|P-value:1.58E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:-3.32|P-value:6.40E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-3.23|P-value:8.58E-4 ID:SGTC_2834|Compound:9001755|FD-Score:3.40|P-value:4.64E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-3.52|P-value:3.23E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.79|P-value:1.10E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-3.19|P-value:9.74E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.38|P-value:4.90E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:3.25|P-value:7.78E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.25|P-value:7.74E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:4.89|P-value:9.69E-7 ID:SGTC_145|Compound:3737-0113|FD-Score:3.34|P-value:5.72E-4 ID:SGTC_264|Compound:promethazine|FD-Score:5.23|P-value:1.82E-7 ID:SGTC_310|Compound:1273-0058|FD-Score:3.61|P-value:2.16E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:-3.19|P-value:9.96E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:-4.67|P-value:2.94E-6 ID:SGTC_409|Compound:arachidonic acid|FD-Score:3.54|P-value:2.83E-4 ID:SGTC_507|Compound:nimesulide|FD-Score:3.43|P-value:4.13E-4 ID:SGTC_512|Compound:prima-1|FD-Score:3.56|P-value:2.58E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:5.73|P-value:1.25E-8 ID:SGTC_575|Compound:k072-0246|FD-Score:3.34|P-value:5.77E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:-3.53|P-value:3.12E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:4.53|P-value:5.16E-6 ID:SGTC_852|Compound:0418-0143|FD-Score:-4.58|P-value:4.39E-6 ID:SGTC_867|Compound:0518-0118|FD-Score:5.35|P-value:9.79E-8 ID:SGTC_982|Compound:1491-0518|FD-Score:-3.57|P-value:2.67E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-7.88|P-value:9.50E-15 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.22|P-value:8.52E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.27|P-value:7.22E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:4.21|P-value:2.05E-5 ID:SGTC_1588|Compound:farnesol|FD-Score:4.14|P-value:2.81E-5 ID:SGTC_1602|Compound:st001878|FD-Score:3.51|P-value:3.18E-4 ID:SGTC_1621|Compound:st003705|FD-Score:-3.29|P-value:7.09E-4 ID:SGTC_1896|Compound:st058441|FD-Score:-3.70|P-value:1.66E-4 ID:SGTC_1923|Compound:st058458|FD-Score:-3.26|P-value:7.83E-4 ID:SGTC_1933|Compound:st074711|FD-Score:6.72|P-value:3.06E-11 ID:SGTC_1962|Compound:st076595|FD-Score:4.62|P-value:3.43E-6 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:-3.59|P-value:2.44E-4 ID:SGTC_1966|Compound:st070145|FD-Score:3.95|P-value:5.94E-5 ID:SGTC_1980|Compound:st071811|FD-Score:-3.75|P-value:1.36E-4 ID:SGTC_1984|Compound:st072354|FD-Score:3.45|P-value:3.83E-4 ID:SGTC_2068|Compound:5226020|FD-Score:3.71|P-value:1.50E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.34|P-value:1.17E-5 ID:SGTC_2161|Compound:5662504|FD-Score:-3.24|P-value:8.48E-4 ID:SGTC_2163|Compound:5671233|FD-Score:-3.41|P-value:4.73E-4 ID:SGTC_2173|Compound:5763948|FD-Score:-3.20|P-value:9.43E-4 ID:SGTC_2181|Compound:6316240|FD-Score:3.18|P-value:9.75E-4 ID:SGTC_2239|Compound:6685329|FD-Score:4.01|P-value:4.65E-5 ID:SGTC_2336|Compound:9001819|FD-Score:-3.45|P-value:4.08E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.21|P-value:8.94E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:4.30|P-value:1.40E-5 ID:SGTC_2555|Compound:cholic acid|FD-Score:3.70|P-value:1.58E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:-3.32|P-value:6.40E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-3.23|P-value:8.58E-4 ID:SGTC_2834|Compound:9001755|FD-Score:3.40|P-value:4.64E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-3.52|P-value:3.23E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.79|P-value:1.10E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-3.19|P-value:9.74E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.38|P-value:4.90E-4

Top fitness defect scores for YDR494W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1933 st074711 6.72 15.9 µM 3.06E-11 8 9.00E-12
2 SGTC_574 1315-0397 5.73 40.0 µM 1.25E-8 8 5.14E-9
3 SGTC_867 0518-0118 5.35 155.0 µM 9.79E-8 DNA intercalators 6 4.51E-8
4 SGTC_264 promethazine 5.23 12.5 mM 1.82E-7 DNA intercalators 27 8.68E-8
5 SGTC_143 4032-1194 4.89 15.7 µM 9.69E-7 33 5.06E-7
6 SGTC_1962 st076595 4.62 64.5 µM 3.43E-6 redox potentiating 14 1.92E-6
7 SGTC_768 0384-0033 4.53 77.3 µM 5.16E-6 fatty acid desaturase (OLE1) 67 2.95E-6
8 SGTC_2150 5556613 4.34 200.0 µM 1.17E-5 11 6.98E-6
9 SGTC_2544 sphondin 4.30 68.5 µM 1.40E-5 RNA pol III & RNase P/MRP 18 8.46E-6
10 SGTC_1582 3',3',6'-trihydroxyflavone 4.21 74.0 µM 2.05E-5 8 1.26E-5
11 SGTC_1588 farnesol 4.14 29.9 µM 2.81E-5 5 1.76E-5
12 SGTC_2239 6685329 4.01 187.6 µM 4.65E-5 28 3.00E-5
13 SGTC_1966 st070145 3.95 16.1 µM 5.94E-5 23 3.87E-5
14 SGTC_3033 9090887 3.79 49.5 µM 1.10E-4 fatty acid desaturase (OLE1) 31 7.43E-5
15 SGTC_2068 5226020 3.71 147.0 µM 1.50E-4 34 1.03E-4
16 SGTC_2555 cholic acid 3.70 100.0 µM 1.58E-4 22 1.09E-4
17 SGTC_310 1273-0058 3.61 1.1 µM 2.16E-4 mitochondrial stress 273 1.51E-4
18 SGTC_512 prima-1 3.56 270.0 µM 2.58E-4 DNA intercalators 36 1.82E-4
19 SGTC_409 arachidonic acid 3.54 100.0 µM 2.83E-4 26 2.00E-4
20 SGTC_1602 st001878 3.51 87.6 µM 3.18E-4 33 2.27E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.171 2.20E-23 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.164 1.17E-21 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.162 3.73E-21 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.157 8.46E-20 YGL041C_d YGL041C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.154 3.62E-19 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.145 2.72E-17 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.140 3.82E-16 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.116 1.59E-11 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.116 1.94E-11 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.113 4.91E-11 YBR170C NPL4 hom Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP)
0.111 1.07E-10 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.108 3.70E-10 YNL264C PDR17 hom Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition
0.106 7.17E-10 YER003C PMI40 het Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
0.105 1.09E-9 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.105 1.12E-9 YHR172W SPC97 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque