YDR498C / SEC20

Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR498C / SEC20

Click on Significant Values for Screen Details ID:SGTC_11|Compound:0438-0307|FD-Score:3.99|P-value:1.38E-5 ID:SGTC_49|Compound:0717-0965|FD-Score:3.71|P-value:4.82E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:3.42|P-value:1.62E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.60|P-value:7.00E-7 ID:SGTC_106|Compound:0092-0003|FD-Score:3.42|P-value:1.62E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:3.78|P-value:3.60E-5 ID:SGTC_147|Compound:4466-0538|FD-Score:3.18|P-value:4.15E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.15|P-value:5.53E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.97|P-value:1.49E-5 ID:SGTC_596|Compound:1155-0152|FD-Score:3.68|P-value:5.54E-5 ID:SGTC_600|Compound:1495-0418|FD-Score:3.54|P-value:1.00E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:3.23|P-value:3.44E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:2.99|P-value:8.21E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:-3.33|P-value:2.73E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:-3.20|P-value:4.49E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.33|P-value:2.31E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.79|P-value:4.27E-5 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.03|P-value:7.08E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.52|P-value:1.09E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.05|P-value:6.70E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.43|P-value:1.54E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.85|P-value:2.57E-5 ID:SGTC_1875|Compound:nocodazole|FD-Score:3.06|P-value:6.45E-4 ID:SGTC_1938|Compound:st074717|FD-Score:3.19|P-value:3.92E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-3.61|P-value:9.19E-5 ID:SGTC_2253|Compound:7938677|FD-Score:-3.14|P-value:5.73E-4 ID:SGTC_2258|Compound:7960471|FD-Score:3.75|P-value:4.08E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.30|P-value:3.12E-4 ID:SGTC_2416|Compound:tofa|FD-Score:3.22|P-value:3.53E-4 ID:SGTC_2456|Compound:5770359|FD-Score:-3.53|P-value:1.28E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-4.57|P-value:1.05E-6 ID:SGTC_2645|Compound:abamectin|FD-Score:3.28|P-value:2.82E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.28|P-value:2.80E-4 ID:SGTC_2810|Compound:7959871|FD-Score:5.92|P-value:2.80E-10 ID:SGTC_2811|Compound:7967822|FD-Score:3.76|P-value:3.86E-5 ID:SGTC_2858|Compound:9025781|FD-Score:3.05|P-value:6.66E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-6.03|P-value:1.74E-10 ID:SGTC_3129|Compound:9127257|FD-Score:4.10|P-value:8.32E-6 ID:SGTC_3191|Compound:9110352|FD-Score:-5.16|P-value:4.03E-8 ID:SGTC_3200|Compound:9114385|FD-Score:-4.89|P-value:1.90E-7 ID:SGTC_3284|Compound:9108171|FD-Score:2.99|P-value:8.26E-4 ID:SGTC_3323|Compound:9138564|FD-Score:-3.21|P-value:4.33E-4 ID:SGTC_3327|Compound:9140648|FD-Score:-4.04|P-value:1.36E-5 ID:SGTC_11|Compound:0438-0307|FD-Score:3.99|P-value:1.38E-5 ID:SGTC_49|Compound:0717-0965|FD-Score:3.71|P-value:4.82E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:3.42|P-value:1.62E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.60|P-value:7.00E-7 ID:SGTC_106|Compound:0092-0003|FD-Score:3.42|P-value:1.62E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:3.78|P-value:3.60E-5 ID:SGTC_147|Compound:4466-0538|FD-Score:3.18|P-value:4.15E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.15|P-value:5.53E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.97|P-value:1.49E-5 ID:SGTC_596|Compound:1155-0152|FD-Score:3.68|P-value:5.54E-5 ID:SGTC_600|Compound:1495-0418|FD-Score:3.54|P-value:1.00E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:3.23|P-value:3.44E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:2.99|P-value:8.21E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:-3.33|P-value:2.73E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:-3.20|P-value:4.49E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.33|P-value:2.31E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.79|P-value:4.27E-5 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.03|P-value:7.08E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.52|P-value:1.09E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.05|P-value:6.70E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.43|P-value:1.54E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.85|P-value:2.57E-5 ID:SGTC_1875|Compound:nocodazole|FD-Score:3.06|P-value:6.45E-4 ID:SGTC_1938|Compound:st074717|FD-Score:3.19|P-value:3.92E-4 ID:SGTC_2015|Compound:5119600|FD-Score:-3.61|P-value:9.19E-5 ID:SGTC_2253|Compound:7938677|FD-Score:-3.14|P-value:5.73E-4 ID:SGTC_2258|Compound:7960471|FD-Score:3.75|P-value:4.08E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.30|P-value:3.12E-4 ID:SGTC_2416|Compound:tofa|FD-Score:3.22|P-value:3.53E-4 ID:SGTC_2456|Compound:5770359|FD-Score:-3.53|P-value:1.28E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-4.57|P-value:1.05E-6 ID:SGTC_2645|Compound:abamectin|FD-Score:3.28|P-value:2.82E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.28|P-value:2.80E-4 ID:SGTC_2810|Compound:7959871|FD-Score:5.92|P-value:2.80E-10 ID:SGTC_2811|Compound:7967822|FD-Score:3.76|P-value:3.86E-5 ID:SGTC_2858|Compound:9025781|FD-Score:3.05|P-value:6.66E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-6.03|P-value:1.74E-10 ID:SGTC_3129|Compound:9127257|FD-Score:4.10|P-value:8.32E-6 ID:SGTC_3191|Compound:9110352|FD-Score:-5.16|P-value:4.03E-8 ID:SGTC_3200|Compound:9114385|FD-Score:-4.89|P-value:1.90E-7 ID:SGTC_3284|Compound:9108171|FD-Score:2.99|P-value:8.26E-4 ID:SGTC_3323|Compound:9138564|FD-Score:-3.21|P-value:4.33E-4 ID:SGTC_3327|Compound:9140648|FD-Score:-4.04|P-value:1.36E-5

Top fitness defect scores for YDR498C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2810 7959871 5.92 45.5 µM 2.80E-10 copper-dependent oxidative stress 7 1.65E-9 0.18
2 SGTC_103 0844-0104 4.60 51.8 µM 7.00E-7 ERAD & cell cycle 42 2.14E-6 0.00
3 SGTC_3129 9127257 4.10 49.5 µM 8.32E-6 6 2.06E-5 0.25
4 SGTC_11 0438-0307 3.99 1.3 µM 1.38E-5 5 3.28E-5 0.05
5 SGTC_422 cantharidin 3.97 155.0 µM 1.49E-5 cell wall signaling 7 3.52E-5 0.23
6 SGTC_1843 st055809 3.85 45.5 µM 2.57E-5 7 5.79E-5 0.08
7 SGTC_143 4032-1194 3.78 15.7 µM 3.60E-5 18 7.88E-5 0.02
8 SGTC_2811 7967822 3.76 32.5 µM 3.86E-5 6 8.41E-5 0.09
9 SGTC_2258 7960471 3.75 200.0 µM 4.08E-5 5 8.86E-5 0.30
10 SGTC_49 0717-0965 3.71 32.1 µM 4.82E-5 60S ribosome export 11 1.03E-4 0.06
11 SGTC_596 1155-0152 3.68 116.0 µM 5.54E-5 TRP & mitochondrial translation 10 1.17E-4 0.22
12 SGTC_600 1495-0418 3.54 41.6 µM 1.00E-4 RPP1 & pyrimidine depletion 7 2.02E-4 0.02
13 SGTC_1472 k841-0093 3.52 193.0 µM 1.09E-4 1 2.18E-4 0.18
14 SGTC_1807 st050092 3.43 58.9 µM 1.54E-4 60S ribosome export 10 2.98E-4 0.19
15 SGTC_106 0092-0003 3.42 11.6 µM 1.62E-4 copper-dependent oxidative stress 7 3.13E-4 0.06
16 SGTC_68 0986-0284 3.42 82.8 µM 1.62E-4 19 3.13E-4 0.15
17 SGTC_1172 0370-0043 3.33 85.0 µM 2.31E-4 9 4.34E-4 0.03
18 SGTC_2765 myclobutanil 3.28 7.7 µM 2.80E-4 azole & statin 19 5.17E-4 0.05
19 SGTC_2645 abamectin 3.28 100.0 µM 2.82E-4 heme requiring 20 5.21E-4 0.01
20 SGTC_676 falcarinol 3.23 140.0 µM 3.44E-4 22 6.25E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.124 6.15E-13 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.122 1.46E-12 YDL122W UBP1 hom Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
0.115 2.37E-11 YGR049W SCM4 hom Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation
0.112 9.55E-11 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.105 1.09E-9 YDR023W SES1 het Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
0.104 1.67E-9 YDR418W RPL12B hom Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication
0.102 2.99E-9 YER038C KRE29 het Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
0.102 3.37E-9 YAR008W SEN34 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
0.102 3.84E-9 YKL042W SPC42 het Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
0.101 4.63E-9 YHR197W RIX1 het Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
0.100 6.28E-9 YBR193C MED8 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.099 7.74E-9 YML098W TAF13 het TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
0.099 8.66E-9 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.098 1.30E-8 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.097 1.62E-8 YKR063C LAS1 het Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability