YDR499W / LCD1

Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR499W / LCD1

Click on Significant Values for Screen Details ID:SGTC_280|Compound:3448-3607|FD-Score:3.00|P-value:1.93E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:-2.86|P-value:2.95E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.19|P-value:8.11E-5 ID:SGTC_522|Compound:1273-0010|FD-Score:2.65|P-value:8.80E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:-2.79|P-value:4.15E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:7.34|P-value:1.50E-18 ID:SGTC_614|Compound:0462-0418|FD-Score:-4.71|P-value:8.60E-9 ID:SGTC_620|Compound:0109-0052|FD-Score:-2.64|P-value:7.79E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:-3.64|P-value:6.46E-6 ID:SGTC_631|Compound:k072-0230|FD-Score:-3.77|P-value:3.25E-6 ID:SGTC_636|Compound:0kpi-0074|FD-Score:2.79|P-value:4.95E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:3.46|P-value:2.09E-5 ID:SGTC_811|Compound:0416-0114|FD-Score:2.78|P-value:5.19E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.29|P-value:5.08E-5 ID:SGTC_824|Compound:1304-0008|FD-Score:3.20|P-value:7.62E-5 ID:SGTC_831|Compound:k061-1017|FD-Score:3.19|P-value:8.27E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:2.88|P-value:3.39E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:2.80|P-value:4.73E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.23|P-value:2.10E-7 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.87|P-value:2.90E-9 ID:SGTC_1125|Compound:tnp00248|FD-Score:8.83|P-value:5.20E-26 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-4.26|P-value:1.76E-7 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.50|P-value:1.39E-5 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.00|P-value:1.95E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.44|P-value:2.33E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:2.77|P-value:5.28E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:2.96|P-value:2.38E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.39|P-value:3.01E-5 ID:SGTC_1524|Compound:corynanthine|FD-Score:2.62|P-value:9.95E-4 ID:SGTC_1620|Compound:st003704|FD-Score:2.77|P-value:5.25E-4 ID:SGTC_1710|Compound:st031758|FD-Score:3.16|P-value:9.15E-5 ID:SGTC_1904|Compound:st060225|FD-Score:-2.77|P-value:4.35E-4 ID:SGTC_1979|Compound:st071809|FD-Score:2.70|P-value:7.10E-4 ID:SGTC_2261|Compound:9010538|FD-Score:2.93|P-value:2.69E-4 ID:SGTC_2292|Compound:7671323|FD-Score:2.97|P-value:2.26E-4 ID:SGTC_2309|Compound:7664088|FD-Score:2.62|P-value:9.82E-4 ID:SGTC_2314|Compound:7727897|FD-Score:-2.78|P-value:4.16E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.12|P-value:1.12E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-2.61|P-value:8.64E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.10|P-value:1.25E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.11|P-value:9.70E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.03|P-value:1.69E-4 ID:SGTC_2784|Compound:7567673|FD-Score:-2.79|P-value:4.04E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.82|P-value:3.07E-6 ID:SGTC_2965|Compound:9082772|FD-Score:-5.30|P-value:1.19E-10 ID:SGTC_3135|Compound:9127742|FD-Score:-2.64|P-value:7.67E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:3.00|P-value:1.93E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:-2.86|P-value:2.95E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.19|P-value:8.11E-5 ID:SGTC_522|Compound:1273-0010|FD-Score:2.65|P-value:8.80E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:-2.79|P-value:4.15E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:7.34|P-value:1.50E-18 ID:SGTC_614|Compound:0462-0418|FD-Score:-4.71|P-value:8.60E-9 ID:SGTC_620|Compound:0109-0052|FD-Score:-2.64|P-value:7.79E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:-3.64|P-value:6.46E-6 ID:SGTC_631|Compound:k072-0230|FD-Score:-3.77|P-value:3.25E-6 ID:SGTC_636|Compound:0kpi-0074|FD-Score:2.79|P-value:4.95E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:3.46|P-value:2.09E-5 ID:SGTC_811|Compound:0416-0114|FD-Score:2.78|P-value:5.19E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.29|P-value:5.08E-5 ID:SGTC_824|Compound:1304-0008|FD-Score:3.20|P-value:7.62E-5 ID:SGTC_831|Compound:k061-1017|FD-Score:3.19|P-value:8.27E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:2.88|P-value:3.39E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:2.80|P-value:4.73E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.23|P-value:2.10E-7 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.87|P-value:2.90E-9 ID:SGTC_1125|Compound:tnp00248|FD-Score:8.83|P-value:5.20E-26 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-4.26|P-value:1.76E-7 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.50|P-value:1.39E-5 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.00|P-value:1.95E-4 ID:SGTC_1222|Compound:0366-0074|FD-Score:3.44|P-value:2.33E-5 ID:SGTC_1248|Compound:0469-0687|FD-Score:2.77|P-value:5.28E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:2.96|P-value:2.38E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.39|P-value:3.01E-5 ID:SGTC_1524|Compound:corynanthine|FD-Score:2.62|P-value:9.95E-4 ID:SGTC_1620|Compound:st003704|FD-Score:2.77|P-value:5.25E-4 ID:SGTC_1710|Compound:st031758|FD-Score:3.16|P-value:9.15E-5 ID:SGTC_1904|Compound:st060225|FD-Score:-2.77|P-value:4.35E-4 ID:SGTC_1979|Compound:st071809|FD-Score:2.70|P-value:7.10E-4 ID:SGTC_2261|Compound:9010538|FD-Score:2.93|P-value:2.69E-4 ID:SGTC_2292|Compound:7671323|FD-Score:2.97|P-value:2.26E-4 ID:SGTC_2309|Compound:7664088|FD-Score:2.62|P-value:9.82E-4 ID:SGTC_2314|Compound:7727897|FD-Score:-2.78|P-value:4.16E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.12|P-value:1.12E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-2.61|P-value:8.64E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.10|P-value:1.25E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.11|P-value:9.70E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.03|P-value:1.69E-4 ID:SGTC_2784|Compound:7567673|FD-Score:-2.79|P-value:4.04E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.82|P-value:3.07E-6 ID:SGTC_2965|Compound:9082772|FD-Score:-5.30|P-value:1.19E-10 ID:SGTC_3135|Compound:9127742|FD-Score:-2.64|P-value:7.67E-4

Top fitness defect scores for YDR499W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1125 tnp00248 8.83 1.3 µM 5.20E-26 2 5.46E-19 2.71
2 SGTC_609 0986-0026 7.34 86.3 µM 1.50E-18 calcium & mitochondrial duress 4 1.09E-13 0.44
3 SGTC_2791 5663188 3.82 71.4 µM 3.07E-6 RPP1 & pyrimidine depletion 11 6.70E-5 0.33
4 SGTC_663 1486-0733 3.46 13.9 µM 2.09E-5 cell wall signaling 11 2.68E-4 0.10
5 SGTC_1222 0366-0074 3.44 1.2 µM 2.33E-5 TSC3-RPN4 6 2.89E-4 0.19
6 SGTC_1379 2144-0755 3.39 36.5 µM 3.01E-5 10 3.48E-4 0.08
7 SGTC_813 k007-0158 3.29 173.0 µM 5.08E-5 fatty acid desaturase (OLE1) 13 5.08E-4 0.00
8 SGTC_824 1304-0008 3.20 100.0 µM 7.62E-5 DNA damage response 13 6.82E-4 0.01
9 SGTC_451 chloral hydrate 3.19 100.0 µM 8.11E-5 31 7.14E-4 0.03
10 SGTC_831 k061-1017 3.19 217.0 µM 8.27E-5 translation 21 7.24E-4 0.08
11 SGTC_1710 st031758 3.16 61.5 µM 9.15E-5 10 7.79E-4 0.02
12 SGTC_2367 9070478 3.12 135.4 µM 1.12E-4 cell wall 22 9.01E-4 0.03
13 SGTC_2444 5485484 3.10 36.4 µM 1.25E-4 17 9.78E-4 0.01
14 SGTC_2693 st077581 3.03 40.7 µM 1.69E-4 12 0.00122 0.00
15 SGTC_280 3448-3607 3.00 3.0 µM 1.93E-4 60S ribosome export 8 0.00134 0.06
16 SGTC_1221 2958-7315 3.00 257.0 µM 1.95E-4 11 0.00135 0.02
17 SGTC_2292 7671323 2.97 168.1 µM 2.26E-4 RNA processing & uracil transport 26 0.00150 0.04
18 SGTC_1372 2752-0194 2.96 270.0 µM 2.38E-4 9 0.00156 0.00
19 SGTC_2261 9010538 2.93 184.7 µM 2.69E-4 16 0.00170 0.01
20 SGTC_939 1165-0479 2.88 45.3 µM 3.39E-4 20 0.00202 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.249 1.02E-48 YML074C FPR3 hom Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p
0.200 1.70E-31 YDR411C DFM1 hom Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p
0.191 8.66E-29 YBR090C_p YBR090C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.191 8.57E-29 YIL046W-A_p YIL046W-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.179 1.72E-25 YJR127C RSF2 hom Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.176 8.67E-25 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.173 6.27E-24 YHR166C CDC23 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.167 2.03E-22 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.166 5.00E-22 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.160 1.08E-20 YBL063W KIP1 hom Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
0.155 1.53E-19 YMR104C YPK2 hom Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication
0.154 2.97E-19 YGR185C TYS1 het Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
0.151 1.36E-18 YOR062C_p YOR062C_p hom Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
0.149 4.67E-18 YPL217C BMS1 het GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
0.146 1.62E-17 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing