YDR508C / GNP1

High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR508C / GNP1

Click on Significant Values for Screen Details ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.27|P-value:6.95E-9 ID:SGTC_287|Compound:k060-0039|FD-Score:2.97|P-value:6.28E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.17|P-value:4.56E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:3.36|P-value:1.41E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.13|P-value:1.66E-8 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.19|P-value:3.14E-6 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-3.04|P-value:7.33E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-6.05|P-value:8.53E-11 ID:SGTC_501|Compound:nsc-95397|FD-Score:-3.85|P-value:2.61E-5 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:-3.55|P-value:9.86E-5 ID:SGTC_511|Compound:blebbistatin|FD-Score:-4.73|P-value:3.27E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.70|P-value:3.16E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:2.97|P-value:6.31E-4 ID:SGTC_1096|Compound:rimcazole|FD-Score:-4.08|P-value:9.00E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.72|P-value:8.23E-10 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.59|P-value:5.11E-5 ID:SGTC_1192|Compound:1488-0095|FD-Score:-3.20|P-value:4.09E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:-2.98|P-value:9.22E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.36|P-value:1.41E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:3.42|P-value:1.08E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.18|P-value:2.90E-4 ID:SGTC_1412|Compound:3909-8563|FD-Score:3.17|P-value:2.91E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:-4.24|P-value:4.10E-6 ID:SGTC_1429|Compound:4049-0205|FD-Score:-2.99|P-value:8.87E-4 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.11|P-value:3.77E-4 ID:SGTC_1440|Compound:4100-1550|FD-Score:-4.04|P-value:1.08E-5 ID:SGTC_1712|Compound:st032288|FD-Score:3.12|P-value:3.61E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.69|P-value:3.31E-5 ID:SGTC_1760|Compound:st045453|FD-Score:3.13|P-value:3.44E-4 ID:SGTC_1800|Compound:st050079|FD-Score:2.99|P-value:6.00E-4 ID:SGTC_1803|Compound:st050068|FD-Score:2.93|P-value:7.46E-4 ID:SGTC_1903|Compound:st060211|FD-Score:3.38|P-value:1.25E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.24|P-value:2.28E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.99|P-value:8.93E-4 ID:SGTC_2024|Compound:5144227|FD-Score:-2.97|P-value:9.69E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-3.33|P-value:2.42E-4 ID:SGTC_2179|Compound:6298986|FD-Score:3.55|P-value:6.04E-5 ID:SGTC_2180|Compound:6301957|FD-Score:4.09|P-value:5.17E-6 ID:SGTC_2182|Compound:6325192|FD-Score:3.24|P-value:2.23E-4 ID:SGTC_2359|Compound:9039622|FD-Score:3.97|P-value:9.10E-6 ID:SGTC_2473|Compound:5854593|FD-Score:-3.03|P-value:7.68E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:-3.27|P-value:3.08E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:4.04|P-value:6.58E-6 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:-3.10|P-value:5.96E-4 ID:SGTC_2537|Compound:larixol|FD-Score:2.91|P-value:7.87E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.30|P-value:1.74E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.45|P-value:9.45E-5 ID:SGTC_2591|Compound:skatole|FD-Score:3.12|P-value:3.67E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:-3.13|P-value:5.39E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.58|P-value:9.78E-10 ID:SGTC_2754|Compound:granisetron|FD-Score:3.08|P-value:4.28E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.02|P-value:5.29E-4 ID:SGTC_2862|Compound:9027634|FD-Score:-3.28|P-value:3.00E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.35|P-value:2.30E-4 ID:SGTC_2876|Compound:9059903|FD-Score:2.89|P-value:8.60E-4 ID:SGTC_2978|Compound:9011784|FD-Score:3.30|P-value:1.80E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.05|P-value:6.17E-6 ID:SGTC_3283|Compound:9109332|FD-Score:-3.22|P-value:3.83E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.27|P-value:6.95E-9 ID:SGTC_287|Compound:k060-0039|FD-Score:2.97|P-value:6.28E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.17|P-value:4.56E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:3.36|P-value:1.41E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.13|P-value:1.66E-8 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.19|P-value:3.14E-6 ID:SGTC_495|Compound:decylubiquinone|FD-Score:-3.04|P-value:7.33E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-6.05|P-value:8.53E-11 ID:SGTC_501|Compound:nsc-95397|FD-Score:-3.85|P-value:2.61E-5 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:-3.55|P-value:9.86E-5 ID:SGTC_511|Compound:blebbistatin|FD-Score:-4.73|P-value:3.27E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.70|P-value:3.16E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:2.97|P-value:6.31E-4 ID:SGTC_1096|Compound:rimcazole|FD-Score:-4.08|P-value:9.00E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.72|P-value:8.23E-10 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.59|P-value:5.11E-5 ID:SGTC_1192|Compound:1488-0095|FD-Score:-3.20|P-value:4.09E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:-2.98|P-value:9.22E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.36|P-value:1.41E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:3.42|P-value:1.08E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.18|P-value:2.90E-4 ID:SGTC_1412|Compound:3909-8563|FD-Score:3.17|P-value:2.91E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:-4.24|P-value:4.10E-6 ID:SGTC_1429|Compound:4049-0205|FD-Score:-2.99|P-value:8.87E-4 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.11|P-value:3.77E-4 ID:SGTC_1440|Compound:4100-1550|FD-Score:-4.04|P-value:1.08E-5 ID:SGTC_1712|Compound:st032288|FD-Score:3.12|P-value:3.61E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.69|P-value:3.31E-5 ID:SGTC_1760|Compound:st045453|FD-Score:3.13|P-value:3.44E-4 ID:SGTC_1800|Compound:st050079|FD-Score:2.99|P-value:6.00E-4 ID:SGTC_1803|Compound:st050068|FD-Score:2.93|P-value:7.46E-4 ID:SGTC_1903|Compound:st060211|FD-Score:3.38|P-value:1.25E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.24|P-value:2.28E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.99|P-value:8.93E-4 ID:SGTC_2024|Compound:5144227|FD-Score:-2.97|P-value:9.69E-4 ID:SGTC_2165|Compound:5727197|FD-Score:-3.33|P-value:2.42E-4 ID:SGTC_2179|Compound:6298986|FD-Score:3.55|P-value:6.04E-5 ID:SGTC_2180|Compound:6301957|FD-Score:4.09|P-value:5.17E-6 ID:SGTC_2182|Compound:6325192|FD-Score:3.24|P-value:2.23E-4 ID:SGTC_2359|Compound:9039622|FD-Score:3.97|P-value:9.10E-6 ID:SGTC_2473|Compound:5854593|FD-Score:-3.03|P-value:7.68E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:-3.27|P-value:3.08E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:4.04|P-value:6.58E-6 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:-3.10|P-value:5.96E-4 ID:SGTC_2537|Compound:larixol|FD-Score:2.91|P-value:7.87E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.30|P-value:1.74E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.45|P-value:9.45E-5 ID:SGTC_2591|Compound:skatole|FD-Score:3.12|P-value:3.67E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:-3.13|P-value:5.39E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.58|P-value:9.78E-10 ID:SGTC_2754|Compound:granisetron|FD-Score:3.08|P-value:4.28E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.02|P-value:5.29E-4 ID:SGTC_2862|Compound:9027634|FD-Score:-3.28|P-value:3.00E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.35|P-value:2.30E-4 ID:SGTC_2876|Compound:9059903|FD-Score:2.89|P-value:8.60E-4 ID:SGTC_2978|Compound:9011784|FD-Score:3.30|P-value:1.80E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.05|P-value:6.17E-6 ID:SGTC_3283|Compound:9109332|FD-Score:-3.22|P-value:3.83E-4

Top fitness defect scores for YDR508C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2688 kanamycin b 5.58 96.7 µM 9.78E-10 calcium & mitochondrial duress 190 1.20E-8
2 SGTC_221 trifluoperazine 5.27 10.3 µM 6.95E-9 NEO1-PIK1 153 6.69E-8
3 SGTC_445 e6 berbamine 5.13 66.0 µM 1.66E-8 NEO1-PIK1 56 1.43E-7
4 SGTC_449 xestospongin c 4.19 11.2 µM 3.14E-6 NEO1-PIK1 56 1.42E-5
5 SGTC_2180 6301957 4.09 200.0 µM 5.17E-6 RSC complex & mRNA processing 4 2.20E-5
6 SGTC_3037 9091026 4.05 49.5 µM 6.17E-6 NEO1-PIK1 118 2.56E-5
7 SGTC_2514 salinomycin 4.04 42.7 µM 6.58E-6 46 2.71E-5
8 SGTC_2359 9039622 3.97 200.0 µM 9.10E-6 endomembrane recycling 24 3.60E-5
9 SGTC_831 k061-1017 3.70 217.0 µM 3.16E-5 translation 79 1.07E-4
10 SGTC_1754 st042065 3.69 16.1 µM 3.31E-5 NEO1-PIK1 202 1.12E-4
11 SGTC_1143 3454-2408 3.59 5.1 µM 5.11E-5 60S ribosome export 4 1.63E-4
12 SGTC_2179 6298986 3.55 200.0 µM 6.04E-5 heme requiring 26 1.89E-4
13 SGTC_2582 agaric acid 3.45 100.0 µM 9.45E-5 redox potentiating 372 2.80E-4
14 SGTC_1311 1190-0148 3.42 100.0 µM 1.08E-4 azole & statin 45 3.13E-4
15 SGTC_1903 st060211 3.38 78.3 µM 1.25E-4 50 3.57E-4
16 SGTC_1310 1188-0297 3.36 994.0 nM 1.41E-4 azole & statin 51 3.97E-4
17 SGTC_313 3474-0040 3.36 5.0 µM 1.41E-4 NEO1-PIK1 100 3.97E-4
18 SGTC_2568 diosmetin 3.30 100.0 µM 1.74E-4 47 4.77E-4
19 SGTC_2978 9011784 3.30 43.0 µM 1.80E-4 60 4.92E-4
20 SGTC_2182 6325192 3.24 200.0 µM 2.23E-4 Golgi 31 5.94E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.260 4.48E-53 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.257 6.63E-52 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.234 8.88E-43 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.218 1.44E-37 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.215 1.60E-36 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.209 1.58E-34 YCL035C GRX1 hom Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.203 1.22E-32 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.199 2.57E-31 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.197 9.75E-31 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.194 8.34E-30 YNL079C TPM1 hom Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication
0.193 1.42E-29 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.191 5.05E-29 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.191 5.45E-29 YML094C-A_d YML094C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
0.190 1.03E-28 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.190 1.16E-28 YGL077C HNM1 hom Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol