HIPHOP chemogenomics database

YDR514C

Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR514C / YDR514C

Click on Significant Values for Screen Details

Top fitness defect scores for YDR514C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1251	0490-5699	6.89	265.0 µM	7.94E-13		3	2.88E-12
2	SGTC_3078	9116413	5.63	49.5 µM	3.80E-9	unfolded protein response	11	9.12E-9
3	SGTC_3135	9127742	5.38	49.5 µM	1.64E-8		5	3.68E-8
4	SGTC_946	3434-1334	5.22	87.4 µM	4.17E-8		10	8.93E-8
5	SGTC_2087	5276107	5.00	200.0 µM	1.40E-7		5	2.83E-7
6	SGTC_2086	5274770	4.89	176.6 µM	2.60E-7		10	5.10E-7
7	SGTC_2097	5376186	4.83	200.0 µM	3.48E-7		6	6.73E-7
8	SGTC_2870	9038528	4.23	13.0 µM	7.07E-6		18	1.18E-5
9	SGTC_1404	3909-7820	4.04	48.3 µM	1.68E-5	60S ribosome export	9	2.70E-5
10	SGTC_3307	9126885	4.02	69.3 µM	1.85E-5		30	2.95E-5
11	SGTC_2098	5376439	3.88	882.5 nM	3.34E-5		13	5.18E-5
12	SGTC_355	1311-2252	3.85	105.0 µM	3.79E-5		21	5.85E-5
13	SGTC_2253	7938677	3.84	135.8 µM	3.92E-5		12	6.04E-5
14	SGTC_1249	0481-0015	3.83	10.2 µM	4.24E-5		27	6.51E-5
15	SGTC_3033	9090887	3.77	49.5 µM	5.45E-5	fatty acid desaturase (OLE1)	33	8.27E-5
16	SGTC_1170	0310-0127	3.67	64.8 µM	8.14E-5		36	1.21E-4
17	SGTC_2855	9016525	3.63	58.4 µM	9.77E-5		26	1.44E-4
18	SGTC_2896	9052656	3.59	71.4 µM	1.13E-4	endomembrane recycling	143	1.65E-4
19	SGTC_88	0180-0332	3.53	8.7 µM	1.42E-4		31	2.06E-4
20	SGTC_941	3237-0776	3.50	460.0 µM	1.64E-4		31	2.36E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.154	2.77E-19	YJL171C	YJL171C	hom	GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.140	3.27E-16	YNL339C	YRF1-6	hom	Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
0.124	6.51E-13	YFL051C_p	YFL051C_p	hom	Putative protein of unknown function; YFL051C is not an essential gene
0.122	1.08E-12	YDR502C	SAM2	hom	S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication
0.116	1.75E-11	YDR513W	GRX2	hom	Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication
0.112	7.99E-11	YNL166C	BNI5	hom	Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.112	8.80E-11	YFL044C	OTU1	hom	Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress
0.110	1.64E-10	YDL176W	YDL176W	hom	Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.109	2.26E-10	YDR312W	SSF2	hom	Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.109	2.29E-10	YLR213C	CRR1	hom	Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation
0.108	3.45E-10	YLR356W	ATG33	hom	Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes
0.108	3.31E-10	YGR209C	TRX2	hom	Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress
0.106	7.41E-10	YCR021C	HSP30	hom	Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
0.105	1.10E-9	YPR180W	AOS1	het	Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.104	1.47E-9	YDR521W_d	YDR521W_d	hom	Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol