YDR516C / EMI2

Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR516C / EMI2

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:3.47|P-value:3.48E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.53|P-value:2.82E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.40|P-value:6.42E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-8.27|P-value:1.37E-15 ID:SGTC_62|Compound:0180-0423|FD-Score:3.66|P-value:1.76E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:4.93|P-value:7.98E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:3.52|P-value:2.89E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:3.95|P-value:5.71E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:-3.57|P-value:3.65E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:9.09|P-value:6.65E-19 ID:SGTC_171|Compound:k064-0041|FD-Score:-3.92|P-value:1.01E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:4.94|P-value:7.46E-7 ID:SGTC_304|Compound:1310-0298|FD-Score:-5.45|P-value:1.03E-7 ID:SGTC_308|Compound:4092-1169|FD-Score:-4.07|P-value:5.50E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.07|P-value:5.63E-5 ID:SGTC_329|Compound:k048-0121|FD-Score:3.26|P-value:7.00E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:-3.36|P-value:7.33E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:-3.92|P-value:9.86E-5 ID:SGTC_483|Compound:fpl-64176|FD-Score:-3.47|P-value:5.14E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-5.42|P-value:1.21E-7 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.51|P-value:4.38E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:-3.56|P-value:3.79E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:5.91|P-value:4.58E-9 ID:SGTC_625|Compound:k213-0058|FD-Score:5.63|P-value:2.20E-8 ID:SGTC_660|Compound:4031-1742|FD-Score:3.93|P-value:6.25E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:6.95|P-value:7.84E-12 ID:SGTC_805|Compound:3852-0218|FD-Score:3.80|P-value:1.05E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.85|P-value:8.58E-5 ID:SGTC_824|Compound:1304-0008|FD-Score:5.42|P-value:6.74E-8 ID:SGTC_830|Compound:4076-0286|FD-Score:6.26|P-value:6.14E-10 ID:SGTC_831|Compound:k061-1017|FD-Score:7.42|P-value:3.16E-13 ID:SGTC_832|Compound:4083-0019|FD-Score:7.92|P-value:8.36E-15 ID:SGTC_852|Compound:0418-0143|FD-Score:-4.04|P-value:6.34E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:-3.47|P-value:5.06E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:5.23|P-value:1.83E-7 ID:SGTC_960|Compound:1222-0186|FD-Score:-3.65|P-value:2.75E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.93|P-value:6.35E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:-5.44|P-value:1.12E-7 ID:SGTC_997|Compound:3996-0078|FD-Score:3.32|P-value:5.79E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:4.62|P-value:3.35E-6 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.27|P-value:6.75E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:4.96|P-value:7.03E-7 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-6.09|P-value:3.19E-9 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-4.10|P-value:5.02E-5 ID:SGTC_1635|Compound:st007035|FD-Score:7.22|P-value:1.27E-12 ID:SGTC_1636|Compound:st007707|FD-Score:-4.83|P-value:2.14E-6 ID:SGTC_1638|Compound:st008370|FD-Score:3.34|P-value:5.44E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.65|P-value:2.72E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-4.89|P-value:1.63E-6 ID:SGTC_1834|Compound:st055981|FD-Score:3.50|P-value:3.04E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.56|P-value:2.53E-4 ID:SGTC_1863|Compound:st057641|FD-Score:4.66|P-value:2.79E-6 ID:SGTC_1913|Compound:st060213|FD-Score:3.48|P-value:3.38E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.29|P-value:6.33E-4 ID:SGTC_2194|Compound:5927483|FD-Score:-3.37|P-value:7.24E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:-3.51|P-value:4.47E-4 ID:SGTC_2253|Compound:7938677|FD-Score:3.44|P-value:3.89E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.31|P-value:8.69E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:-3.54|P-value:4.01E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:5.51|P-value:4.19E-8 ID:SGTC_2801|Compound:7907800|FD-Score:3.36|P-value:5.11E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.61|P-value:2.12E-4 ID:SGTC_2938|Compound:9059746|FD-Score:-4.12|P-value:4.53E-5 ID:SGTC_2957|Compound:9076800|FD-Score:-4.64|P-value:5.20E-6 ID:SGTC_2958|Compound:9077254|FD-Score:6.81|P-value:1.88E-11 ID:SGTC_2971|Compound:9092186|FD-Score:-3.94|P-value:9.18E-5 ID:SGTC_2983|Compound:9022671|FD-Score:-3.95|P-value:8.95E-5 ID:SGTC_3266|Compound:9136860|FD-Score:-3.74|P-value:1.94E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:3.47|P-value:3.48E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.53|P-value:2.82E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.40|P-value:6.42E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-8.27|P-value:1.37E-15 ID:SGTC_62|Compound:0180-0423|FD-Score:3.66|P-value:1.76E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:4.93|P-value:7.98E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:3.52|P-value:2.89E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:3.95|P-value:5.71E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:-3.57|P-value:3.65E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:9.09|P-value:6.65E-19 ID:SGTC_171|Compound:k064-0041|FD-Score:-3.92|P-value:1.01E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:4.94|P-value:7.46E-7 ID:SGTC_304|Compound:1310-0298|FD-Score:-5.45|P-value:1.03E-7 ID:SGTC_308|Compound:4092-1169|FD-Score:-4.07|P-value:5.50E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.07|P-value:5.63E-5 ID:SGTC_329|Compound:k048-0121|FD-Score:3.26|P-value:7.00E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:-3.36|P-value:7.33E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:-3.92|P-value:9.86E-5 ID:SGTC_483|Compound:fpl-64176|FD-Score:-3.47|P-value:5.14E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-5.42|P-value:1.21E-7 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.51|P-value:4.38E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:-3.56|P-value:3.79E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:5.91|P-value:4.58E-9 ID:SGTC_625|Compound:k213-0058|FD-Score:5.63|P-value:2.20E-8 ID:SGTC_660|Compound:4031-1742|FD-Score:3.93|P-value:6.25E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:6.95|P-value:7.84E-12 ID:SGTC_805|Compound:3852-0218|FD-Score:3.80|P-value:1.05E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.85|P-value:8.58E-5 ID:SGTC_824|Compound:1304-0008|FD-Score:5.42|P-value:6.74E-8 ID:SGTC_830|Compound:4076-0286|FD-Score:6.26|P-value:6.14E-10 ID:SGTC_831|Compound:k061-1017|FD-Score:7.42|P-value:3.16E-13 ID:SGTC_832|Compound:4083-0019|FD-Score:7.92|P-value:8.36E-15 ID:SGTC_852|Compound:0418-0143|FD-Score:-4.04|P-value:6.34E-5 ID:SGTC_863|Compound:0466-0191|FD-Score:-3.47|P-value:5.06E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:5.23|P-value:1.83E-7 ID:SGTC_960|Compound:1222-0186|FD-Score:-3.65|P-value:2.75E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.93|P-value:6.35E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:-5.44|P-value:1.12E-7 ID:SGTC_997|Compound:3996-0078|FD-Score:3.32|P-value:5.79E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:4.62|P-value:3.35E-6 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.27|P-value:6.75E-4 ID:SGTC_1596|Compound:piperlongumine|FD-Score:4.96|P-value:7.03E-7 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-6.09|P-value:3.19E-9 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-4.10|P-value:5.02E-5 ID:SGTC_1635|Compound:st007035|FD-Score:7.22|P-value:1.27E-12 ID:SGTC_1636|Compound:st007707|FD-Score:-4.83|P-value:2.14E-6 ID:SGTC_1638|Compound:st008370|FD-Score:3.34|P-value:5.44E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.65|P-value:2.72E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-4.89|P-value:1.63E-6 ID:SGTC_1834|Compound:st055981|FD-Score:3.50|P-value:3.04E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.56|P-value:2.53E-4 ID:SGTC_1863|Compound:st057641|FD-Score:4.66|P-value:2.79E-6 ID:SGTC_1913|Compound:st060213|FD-Score:3.48|P-value:3.38E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.29|P-value:6.33E-4 ID:SGTC_2194|Compound:5927483|FD-Score:-3.37|P-value:7.24E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:-3.51|P-value:4.47E-4 ID:SGTC_2253|Compound:7938677|FD-Score:3.44|P-value:3.89E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.31|P-value:8.69E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:-3.54|P-value:4.01E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:5.51|P-value:4.19E-8 ID:SGTC_2801|Compound:7907800|FD-Score:3.36|P-value:5.11E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.61|P-value:2.12E-4 ID:SGTC_2938|Compound:9059746|FD-Score:-4.12|P-value:4.53E-5 ID:SGTC_2957|Compound:9076800|FD-Score:-4.64|P-value:5.20E-6 ID:SGTC_2958|Compound:9077254|FD-Score:6.81|P-value:1.88E-11 ID:SGTC_2971|Compound:9092186|FD-Score:-3.94|P-value:9.18E-5 ID:SGTC_2983|Compound:9022671|FD-Score:-3.95|P-value:8.95E-5 ID:SGTC_3266|Compound:9136860|FD-Score:-3.74|P-value:1.94E-4

Top fitness defect scores for YDR516C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_159 3970-0959 9.09 50.0 µM 6.65E-19 8 4.96E-20
2 SGTC_832 4083-0019 7.92 164.0 µM 8.36E-15 translation 10 1.22E-15
3 SGTC_831 k061-1017 7.42 217.0 µM 3.16E-13 translation 3 5.98E-14
4 SGTC_1635 st007035 7.22 42.4 µM 1.27E-12 109 2.64E-13
5 SGTC_800 3448-7362 6.95 155.0 µM 7.84E-12 translation 26 1.85E-12
6 SGTC_2958 9077254 6.81 16.7 µM 1.88E-11 2 4.73E-12
7 SGTC_830 4076-0286 6.26 57.8 µM 6.14E-10 translation 13 1.96E-10
8 SGTC_576 1073-0113 5.91 22.7 µM 4.58E-9 redox potentiating 21 1.68E-9
9 SGTC_625 k213-0058 5.63 121.0 µM 2.20E-8 translation 42 8.94E-9
10 SGTC_2768 sulfasalazine 5.51 50.2 µM 4.19E-8 3 1.78E-8
11 SGTC_824 1304-0008 5.42 100.0 µM 6.74E-8 DNA damage response 12 2.96E-8
12 SGTC_955 1216-0110 5.23 33.0 µM 1.83E-7 sphingolipid biosynthesis & PDR1 42 8.59E-8
13 SGTC_1596 piperlongumine 4.96 63.0 µM 7.03E-7 3 3.60E-7
14 SGTC_266 formosanin C 4.94 406.0 nM 7.46E-7 sphingolipid biosynthesis & PDR1 46 3.84E-7
15 SGTC_66 0109-0187 4.93 42.4 µM 7.98E-7 22 4.12E-7
16 SGTC_1863 st057641 4.66 20.1 µM 2.79E-6 4 1.56E-6
17 SGTC_1073 triclabendazole 4.62 3.1 µM 3.35E-6 16 1.90E-6
18 SGTC_90 0870-0863 3.95 4.6 µM 5.71E-5 46 3.87E-5
19 SGTC_660 4031-1742 3.93 102.0 µM 6.25E-5 redox potentiating 48 4.26E-5
20 SGTC_963 1269-2853 3.93 151.0 µM 6.35E-5 24 4.33E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.226 5.62E-40 YGL251C HFM1 hom Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity
0.221 1.91E-38 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.220 4.84E-38 YPL208W RKM1 hom SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp)
0.201 7.42E-32 YGL236C MTO1 hom Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants
0.194 7.28E-30 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.190 1.79E-28 YDR281C PHM6 hom Protein of unknown function, expression is regulated by phosphate levels
0.180 6.24E-26 YGL250W RMR1 hom Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
0.172 1.65E-23 YOR305W RRG7 hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene
0.171 2.36E-23 YIL039W TED1 hom Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.170 4.84E-23 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.155 1.58E-19 YBR162C TOS1 hom Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
0.147 1.31E-17 YNR018W RCF2 hom Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
0.143 9.53E-17 YOL100W PKH2 hom Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p
0.137 1.38E-15 YEL029C BUD16 hom Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK)
0.134 8.08E-15 YIL103W DPH1 hom Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p