YDR525W_d / API2

Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR525W / API2

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:4.57|P-value:3.04E-5 ID:SGTC_206|Compound:4546-0025|FD-Score:3.89|P-value:3.26E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.74|P-value:1.56E-5 ID:SGTC_342|Compound:k048-0088|FD-Score:4.67|P-value:2.04E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:-4.67|P-value:1.18E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.52|P-value:2.08E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:4.16|P-value:1.33E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.08|P-value:1.06E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.32|P-value:4.45E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:4.52|P-value:3.63E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.44|P-value:8.73E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.77|P-value:4.79E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:8.11|P-value:3.82E-13 ID:SGTC_800|Compound:3448-7362|FD-Score:6.01|P-value:5.89E-8 ID:SGTC_843|Compound:0310-0051|FD-Score:-3.55|P-value:6.15E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:-3.43|P-value:8.99E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:3.61|P-value:7.94E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:-3.59|P-value:5.47E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:4.15|P-value:1.38E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.82|P-value:4.16E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.55|P-value:9.60E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:4.21|P-value:1.11E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:6.18|P-value:2.60E-8 ID:SGTC_1559|Compound:coumarin 343|FD-Score:4.14|P-value:1.45E-4 ID:SGTC_1615|Compound:st002656|FD-Score:3.98|P-value:2.48E-4 ID:SGTC_1623|Compound:st003709|FD-Score:5.39|P-value:1.03E-6 ID:SGTC_1714|Compound:st033236|FD-Score:6.77|P-value:1.16E-9 ID:SGTC_1754|Compound:st042065|FD-Score:3.77|P-value:4.81E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.63|P-value:7.49E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.64|P-value:1.32E-5 ID:SGTC_1803|Compound:st050068|FD-Score:3.95|P-value:2.68E-4 ID:SGTC_1913|Compound:st060213|FD-Score:8.10|P-value:4.04E-13 ID:SGTC_1991|Compound:st073000|FD-Score:3.93|P-value:2.85E-4 ID:SGTC_2101|Compound:5377231|FD-Score:4.31|P-value:7.91E-5 ID:SGTC_2218|Compound:6965428|FD-Score:-3.56|P-value:5.94E-4 ID:SGTC_2238|Compound:6671620|FD-Score:6.34|P-value:1.12E-8 ID:SGTC_2243|Compound:6634756|FD-Score:4.58|P-value:2.94E-5 ID:SGTC_2253|Compound:7938677|FD-Score:-4.62|P-value:1.43E-5 ID:SGTC_2257|Compound:7960413|FD-Score:5.31|P-value:1.48E-6 ID:SGTC_2259|Compound:7964009|FD-Score:-5.30|P-value:8.35E-7 ID:SGTC_2289|Compound:7946404|FD-Score:6.55|P-value:3.80E-9 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.95|P-value:2.68E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.52|P-value:2.53E-14 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.20|P-value:1.18E-4 ID:SGTC_2782|Compound:7736369|FD-Score:3.54|P-value:9.89E-4 ID:SGTC_2791|Compound:5663188|FD-Score:4.47|P-value:4.37E-5 ID:SGTC_2858|Compound:9025781|FD-Score:-4.55|P-value:1.86E-5 ID:SGTC_2929|Compound:9013348|FD-Score:3.54|P-value:9.77E-4 ID:SGTC_3136|Compound:9095672|FD-Score:5.06|P-value:4.30E-6 ID:SGTC_3138|Compound:9097216|FD-Score:-3.56|P-value:6.01E-4 ID:SGTC_3139|Compound:9095020|FD-Score:3.78|P-value:4.69E-4 ID:SGTC_3197|Compound:9113331|FD-Score:5.11|P-value:3.57E-6 ID:SGTC_3244|Compound:9134489|FD-Score:-3.50|P-value:7.27E-4 ID:SGTC_3324|Compound:9138759|FD-Score:4.30|P-value:8.16E-5 ID:SGTC_15|Compound:0266-0086|FD-Score:4.57|P-value:3.04E-5 ID:SGTC_206|Compound:4546-0025|FD-Score:3.89|P-value:3.26E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.74|P-value:1.56E-5 ID:SGTC_342|Compound:k048-0088|FD-Score:4.67|P-value:2.04E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:-4.67|P-value:1.18E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.52|P-value:2.08E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:4.16|P-value:1.33E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.08|P-value:1.06E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-4.32|P-value:4.45E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:4.52|P-value:3.63E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.44|P-value:8.73E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.77|P-value:4.79E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:8.11|P-value:3.82E-13 ID:SGTC_800|Compound:3448-7362|FD-Score:6.01|P-value:5.89E-8 ID:SGTC_843|Compound:0310-0051|FD-Score:-3.55|P-value:6.15E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:-3.43|P-value:8.99E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:3.61|P-value:7.94E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:-3.59|P-value:5.47E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:4.15|P-value:1.38E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.82|P-value:4.16E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.55|P-value:9.60E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:4.21|P-value:1.11E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:6.18|P-value:2.60E-8 ID:SGTC_1559|Compound:coumarin 343|FD-Score:4.14|P-value:1.45E-4 ID:SGTC_1615|Compound:st002656|FD-Score:3.98|P-value:2.48E-4 ID:SGTC_1623|Compound:st003709|FD-Score:5.39|P-value:1.03E-6 ID:SGTC_1714|Compound:st033236|FD-Score:6.77|P-value:1.16E-9 ID:SGTC_1754|Compound:st042065|FD-Score:3.77|P-value:4.81E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.63|P-value:7.49E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.64|P-value:1.32E-5 ID:SGTC_1803|Compound:st050068|FD-Score:3.95|P-value:2.68E-4 ID:SGTC_1913|Compound:st060213|FD-Score:8.10|P-value:4.04E-13 ID:SGTC_1991|Compound:st073000|FD-Score:3.93|P-value:2.85E-4 ID:SGTC_2101|Compound:5377231|FD-Score:4.31|P-value:7.91E-5 ID:SGTC_2218|Compound:6965428|FD-Score:-3.56|P-value:5.94E-4 ID:SGTC_2238|Compound:6671620|FD-Score:6.34|P-value:1.12E-8 ID:SGTC_2243|Compound:6634756|FD-Score:4.58|P-value:2.94E-5 ID:SGTC_2253|Compound:7938677|FD-Score:-4.62|P-value:1.43E-5 ID:SGTC_2257|Compound:7960413|FD-Score:5.31|P-value:1.48E-6 ID:SGTC_2259|Compound:7964009|FD-Score:-5.30|P-value:8.35E-7 ID:SGTC_2289|Compound:7946404|FD-Score:6.55|P-value:3.80E-9 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.95|P-value:2.68E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.52|P-value:2.53E-14 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.20|P-value:1.18E-4 ID:SGTC_2782|Compound:7736369|FD-Score:3.54|P-value:9.89E-4 ID:SGTC_2791|Compound:5663188|FD-Score:4.47|P-value:4.37E-5 ID:SGTC_2858|Compound:9025781|FD-Score:-4.55|P-value:1.86E-5 ID:SGTC_2929|Compound:9013348|FD-Score:3.54|P-value:9.77E-4 ID:SGTC_3136|Compound:9095672|FD-Score:5.06|P-value:4.30E-6 ID:SGTC_3138|Compound:9097216|FD-Score:-3.56|P-value:6.01E-4 ID:SGTC_3139|Compound:9095020|FD-Score:3.78|P-value:4.69E-4 ID:SGTC_3197|Compound:9113331|FD-Score:5.11|P-value:3.57E-6 ID:SGTC_3244|Compound:9134489|FD-Score:-3.50|P-value:7.27E-4 ID:SGTC_3324|Compound:9138759|FD-Score:4.30|P-value:8.16E-5

Top fitness defect scores for YDR525W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2688 kanamycin b 8.52 96.7 µM 2.53E-14 calcium & mitochondrial duress 50 8.29E-18
2 SGTC_708 3448-5400 8.11 146.0 µM 3.82E-13 4 2.56E-16
3 SGTC_1913 st060213 8.10 96.1 µM 4.04E-13 2 2.74E-16
4 SGTC_1714 st033236 6.77 13.6 µM 1.16E-9 2 6.45E-12
5 SGTC_2289 7946404 6.55 165.1 µM 3.80E-9 30 2.88E-11
6 SGTC_2238 6671620 6.34 73.2 µM 1.12E-8 4 1.13E-10
7 SGTC_1434 4073-0095 6.18 163.0 µM 2.60E-8 2 3.27E-10
8 SGTC_800 3448-7362 6.01 155.0 µM 5.89E-8 translation 48 9.20E-10
9 SGTC_1623 st003709 5.39 20.3 µM 1.03E-6 calcium & mitochondrial duress 19 3.43E-8
10 SGTC_2257 7960413 5.31 200.0 µM 1.48E-6 NEO1-PIK1 22 5.41E-8
11 SGTC_3197 9113331 5.11 49.5 µM 3.57E-6 14 1.65E-7
12 SGTC_3136 9095672 5.06 49.5 µM 4.30E-6 7 2.08E-7
13 SGTC_221 trifluoperazine 4.74 10.3 µM 1.56E-5 NEO1-PIK1 173 1.06E-6
14 SGTC_342 k048-0088 4.67 23.3 µM 2.04E-5 RSC & ERG11 7 1.48E-6
15 SGTC_2243 6634756 4.58 200.0 µM 2.94E-5 9 2.36E-6
16 SGTC_15 0266-0086 4.57 27.1 µM 3.04E-5 endomembrane recycling 41 2.46E-6
17 SGTC_519 k048-0011 4.52 62.8 µM 3.63E-5 12 3.08E-6
18 SGTC_2791 5663188 4.47 71.4 µM 4.37E-5 RPP1 & pyrimidine depletion 14 3.89E-6
19 SGTC_2101 5377231 4.31 179.2 µM 7.91E-5 5 8.24E-6
20 SGTC_3324 9138759 4.30 60.8 µM 8.16E-5 13 8.57E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.249 9.28E-49 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.221 1.80E-38 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.206 1.75E-33 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.205 3.55E-33 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.183 1.45E-26 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.170 3.86E-23 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.168 1.50E-22 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.167 1.72E-22 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.167 1.83E-22 YML115C VAN1 hom Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
0.164 1.07E-21 YOL159C-A YOL159C-A hom Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species
0.164 1.01E-21 YNL079C TPM1 hom Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication
0.162 3.71E-21 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.158 3.12E-20 YPL145C KES1 hom One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.158 4.09E-20 YDR125C ECM18 hom Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication
0.158 4.21E-20 YAR042W SWH1 hom Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction