YDR528W / HLR1

Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A;
HLR1 has a paralog, LRE1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR528W / HLR1

Click on Significant Values for Screen Details ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:-3.01|P-value:3.30E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.39|P-value:6.73E-5 ID:SGTC_385|Compound:1494-0393|FD-Score:-3.60|P-value:2.37E-5 ID:SGTC_555|Compound:4296-0685|FD-Score:2.84|P-value:6.96E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:-3.28|P-value:1.04E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:2.93|P-value:4.90E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:2.90|P-value:5.32E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:2.85|P-value:6.54E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:4.15|P-value:1.40E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.77|P-value:9.13E-4 ID:SGTC_1201|Compound:1889-3372|FD-Score:-3.25|P-value:1.16E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.56|P-value:1.63E-10 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.34|P-value:7.76E-10 ID:SGTC_1703|Compound:st026983|FD-Score:3.16|P-value:1.84E-4 ID:SGTC_1706|Compound:st031321|FD-Score:3.56|P-value:3.05E-5 ID:SGTC_2006|Compound:4011487|FD-Score:3.07|P-value:2.75E-4 ID:SGTC_2011|Compound:4012611|FD-Score:3.22|P-value:1.39E-4 ID:SGTC_2162|Compound:5663267|FD-Score:3.53|P-value:3.39E-5 ID:SGTC_2394|Compound:5221648|FD-Score:3.35|P-value:8.07E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.94|P-value:4.40E-6 ID:SGTC_2429|Compound:5902844|FD-Score:2.76|P-value:9.20E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-2.90|P-value:5.23E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:-2.81|P-value:7.24E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:-3.09|P-value:2.30E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.06|P-value:2.70E-4 ID:SGTC_2709|Compound:st078560|FD-Score:2.91|P-value:5.30E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:2.80|P-value:8.08E-4 ID:SGTC_2929|Compound:9013348|FD-Score:-3.01|P-value:3.22E-4 ID:SGTC_2930|Compound:9013230|FD-Score:5.34|P-value:8.66E-10 ID:SGTC_2936|Compound:9038235|FD-Score:-5.48|P-value:2.86E-10 ID:SGTC_2938|Compound:9059746|FD-Score:3.61|P-value:2.36E-5 ID:SGTC_2978|Compound:9011784|FD-Score:3.35|P-value:7.91E-5 ID:SGTC_3027|Compound:9089541|FD-Score:-2.73|P-value:9.88E-4 ID:SGTC_3084|Compound:9116877|FD-Score:4.57|P-value:1.27E-7 ID:SGTC_3085|Compound:9117376|FD-Score:3.06|P-value:2.86E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.45|P-value:5.04E-5 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:-3.01|P-value:3.30E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.39|P-value:6.73E-5 ID:SGTC_385|Compound:1494-0393|FD-Score:-3.60|P-value:2.37E-5 ID:SGTC_555|Compound:4296-0685|FD-Score:2.84|P-value:6.96E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:-3.28|P-value:1.04E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:2.93|P-value:4.90E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:2.90|P-value:5.32E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:2.85|P-value:6.54E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:4.15|P-value:1.40E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.77|P-value:9.13E-4 ID:SGTC_1201|Compound:1889-3372|FD-Score:-3.25|P-value:1.16E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.56|P-value:1.63E-10 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.34|P-value:7.76E-10 ID:SGTC_1703|Compound:st026983|FD-Score:3.16|P-value:1.84E-4 ID:SGTC_1706|Compound:st031321|FD-Score:3.56|P-value:3.05E-5 ID:SGTC_2006|Compound:4011487|FD-Score:3.07|P-value:2.75E-4 ID:SGTC_2011|Compound:4012611|FD-Score:3.22|P-value:1.39E-4 ID:SGTC_2162|Compound:5663267|FD-Score:3.53|P-value:3.39E-5 ID:SGTC_2394|Compound:5221648|FD-Score:3.35|P-value:8.07E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.94|P-value:4.40E-6 ID:SGTC_2429|Compound:5902844|FD-Score:2.76|P-value:9.20E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-2.90|P-value:5.23E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:-2.81|P-value:7.24E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:-3.09|P-value:2.30E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.06|P-value:2.70E-4 ID:SGTC_2709|Compound:st078560|FD-Score:2.91|P-value:5.30E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:2.80|P-value:8.08E-4 ID:SGTC_2929|Compound:9013348|FD-Score:-3.01|P-value:3.22E-4 ID:SGTC_2930|Compound:9013230|FD-Score:5.34|P-value:8.66E-10 ID:SGTC_2936|Compound:9038235|FD-Score:-5.48|P-value:2.86E-10 ID:SGTC_2938|Compound:9059746|FD-Score:3.61|P-value:2.36E-5 ID:SGTC_2978|Compound:9011784|FD-Score:3.35|P-value:7.91E-5 ID:SGTC_3027|Compound:9089541|FD-Score:-2.73|P-value:9.88E-4 ID:SGTC_3084|Compound:9116877|FD-Score:4.57|P-value:1.27E-7 ID:SGTC_3085|Compound:9117376|FD-Score:3.06|P-value:2.86E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.45|P-value:5.04E-5

Top fitness defect scores for YDR528W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2930 9013230 5.34 14.9 µM 8.66E-10 heme biosynthesis & mitochondrial translocase 13 4.71E-8
2 SGTC_3084 9116877 4.57 49.5 µM 1.27E-7 3 2.43E-6
3 SGTC_1122 capsaicin 4.15 818.6 nM 1.40E-6 21 1.63E-5
4 SGTC_2419 paclitaxel 3.94 166.5 µM 4.40E-6 42 4.04E-5
5 SGTC_2938 9059746 3.61 27.3 µM 2.36E-5 fatty acid desaturase (OLE1) 48 1.53E-4
6 SGTC_1706 st031321 3.56 10.0 µM 3.05E-5 TSC3-RPN4 19 1.88E-4
7 SGTC_2162 5663267 3.53 200.0 µM 3.39E-5 19 2.04E-4
8 SGTC_3259 9138485 3.45 49.5 µM 5.04E-5 23 2.81E-4
9 SGTC_358 0075-0058 3.39 9.4 µM 6.73E-5 96 3.53E-4
10 SGTC_2978 9011784 3.35 43.0 µM 7.91E-5 56 4.02E-4
11 SGTC_2394 5221648 3.35 200.0 µM 8.07E-5 48 4.08E-4
12 SGTC_2011 4012611 3.22 177.0 µM 1.39E-4 mitochondrial processes 55 6.30E-4
13 SGTC_1703 st026983 3.16 48.3 µM 1.84E-4 37 7.87E-4
14 SGTC_2006 4011487 3.07 200.0 µM 2.75E-4 endomembrane recycling 135 0.00109
15 SGTC_3085 9117376 3.06 49.5 µM 2.86E-4 42 0.00112
16 SGTC_773 4358-1457 2.93 44.6 µM 4.90E-4 95 0.00172
17 SGTC_2709 st078560 2.91 56.0 µM 5.30E-4 46 0.00183
18 SGTC_774 k060-0086 2.90 136.0 µM 5.32E-4 67 0.00184
19 SGTC_995 3931-2021 2.85 34.6 µM 6.54E-4 RPP1 & pyrimidine depletion 73 0.00217
20 SGTC_555 4296-0685 2.84 37.0 µM 6.96E-4 ergosterol biosynthesis 49 0.00228

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.298 1.38E-69 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.296 6.58E-69 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.291 9.55E-67 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.280 2.27E-61 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.267 6.52E-56 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.256 3.46E-51 YGR045C_d YGR045C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.252 9.08E-50 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.251 5.54E-49 YCL026C-A FRM2 hom Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.249 1.75E-48 YBR274W CHK1 hom Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.248 3.56E-48 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.246 1.35E-47 YJR146W_p YJR146W_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
0.246 1.36E-47 YML086C ALO1 hom D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress
0.232 5.85E-42 YDR133C_d YDR133C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
0.231 5.76E-42 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.226 5.11E-40 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress