YDR540C / IRC4

Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR540C / IRC4

Click on Significant Values for Screen Details ID:SGTC_17|Compound:0960-0276|FD-Score:4.14|P-value:2.63E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:3.86|P-value:6.03E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.83|P-value:2.58E-11 ID:SGTC_110|Compound:0850-0284|FD-Score:-4.90|P-value:1.86E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:4.29|P-value:1.63E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:22.70|P-value:3.39E-81 ID:SGTC_141|Compound:0443-0274|FD-Score:6.10|P-value:1.59E-7 ID:SGTC_157|Compound:1348-1695|FD-Score:3.74|P-value:8.61E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.03|P-value:3.72E-4 ID:SGTC_319|Compound:1348-1596|FD-Score:3.76|P-value:8.12E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.55|P-value:6.75E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:6.28|P-value:6.97E-8 ID:SGTC_586|Compound:0957-0265|FD-Score:5.46|P-value:2.36E-6 ID:SGTC_673|Compound:1082-0474|FD-Score:10.60|P-value:2.13E-19 ID:SGTC_709|Compound:3807-1509|FD-Score:3.91|P-value:5.19E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:4.49|P-value:8.40E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:4.20|P-value:2.16E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:6.03|P-value:2.17E-7 ID:SGTC_982|Compound:1491-0518|FD-Score:3.90|P-value:5.42E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.73|P-value:3.72E-5 ID:SGTC_999|Compound:4022-0066|FD-Score:5.52|P-value:1.86E-6 ID:SGTC_1002|Compound:4049-0206|FD-Score:6.09|P-value:1.66E-7 ID:SGTC_1050|Compound:3232-1156|FD-Score:6.58|P-value:1.68E-8 ID:SGTC_1094|Compound:duloxetine|FD-Score:-4.35|P-value:1.25E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:4.79|P-value:2.95E-5 ID:SGTC_1139|Compound:k015-0024|FD-Score:-4.91|P-value:1.84E-5 ID:SGTC_1140|Compound:3229-1848|FD-Score:5.07|P-value:1.06E-5 ID:SGTC_1143|Compound:3454-2408|FD-Score:-4.61|P-value:5.26E-5 ID:SGTC_1146|Compound:3579-1023|FD-Score:-4.18|P-value:2.15E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:8.34|P-value:1.33E-12 ID:SGTC_1163|Compound:k060-0048|FD-Score:6.62|P-value:1.39E-8 ID:SGTC_1195|Compound:1502-0147|FD-Score:4.37|P-value:1.23E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:-4.50|P-value:7.71E-5 ID:SGTC_1257|Compound:0669-0121|FD-Score:-4.42|P-value:1.01E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:-4.65|P-value:4.63E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.89|P-value:5.52E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:4.52|P-value:7.69E-5 ID:SGTC_1264|Compound:0783-0040|FD-Score:-6.94|P-value:2.76E-9 ID:SGTC_1351|Compound:1496-0003|FD-Score:4.09|P-value:3.02E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:3.76|P-value:8.05E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.39|P-value:1.16E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.79|P-value:7.58E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:-3.69|P-value:9.49E-4 ID:SGTC_1694|Compound:st024011|FD-Score:4.42|P-value:1.05E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.90|P-value:5.39E-4 ID:SGTC_1707|Compound:st032323|FD-Score:5.17|P-value:7.31E-6 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.30|P-value:1.58E-4 ID:SGTC_1915|Compound:st067105|FD-Score:5.18|P-value:6.92E-6 ID:SGTC_1930|Compound:st074634|FD-Score:4.19|P-value:2.21E-4 ID:SGTC_1964|Compound:st076914|FD-Score:3.94|P-value:4.76E-4 ID:SGTC_2026|Compound:5148700|FD-Score:6.39|P-value:4.16E-8 ID:SGTC_2043|Compound:5101342|FD-Score:4.31|P-value:1.50E-4 ID:SGTC_2264|Compound:7948757|FD-Score:3.83|P-value:6.60E-4 ID:SGTC_2289|Compound:7946404|FD-Score:6.10|P-value:1.57E-7 ID:SGTC_2290|Compound:7904846|FD-Score:4.15|P-value:2.49E-4 ID:SGTC_2340|Compound:7987427|FD-Score:-4.36|P-value:1.22E-4 ID:SGTC_2361|Compound:9034121|FD-Score:4.24|P-value:1.93E-4 ID:SGTC_2376|Compound:9072922|FD-Score:3.99|P-value:4.15E-4 ID:SGTC_2396|Compound:5342950|FD-Score:4.29|P-value:1.62E-4 ID:SGTC_2451|Compound:5856223|FD-Score:4.26|P-value:1.80E-4 ID:SGTC_2479|Compound:5617353|FD-Score:4.32|P-value:1.49E-4 ID:SGTC_2487|Compound:5918373|FD-Score:-4.49|P-value:8.03E-5 ID:SGTC_2495|Compound:cafestol|FD-Score:4.77|P-value:3.14E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.73|P-value:8.82E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:5.82|P-value:5.23E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:11.30|P-value:1.76E-21 ID:SGTC_2645|Compound:abamectin|FD-Score:-3.86|P-value:5.85E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:4.34|P-value:1.37E-4 ID:SGTC_2827|Compound:7993847|FD-Score:4.48|P-value:8.59E-5 ID:SGTC_2845|Compound:9003024|FD-Score:4.01|P-value:3.90E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.53|P-value:6.83E-5 ID:SGTC_2938|Compound:9059746|FD-Score:-4.37|P-value:1.20E-4 ID:SGTC_3013|Compound:9080133|FD-Score:-4.15|P-value:2.39E-4 ID:SGTC_3044|Compound:9093193|FD-Score:-4.92|P-value:1.76E-5 ID:SGTC_3049|Compound:9092223|FD-Score:3.96|P-value:4.51E-4 ID:SGTC_3052|Compound:9092717|FD-Score:5.81|P-value:5.62E-7 ID:SGTC_3203|Compound:9113543|FD-Score:-3.80|P-value:6.98E-4 ID:SGTC_3208|Compound:9114115|FD-Score:5.62|P-value:1.24E-6 ID:SGTC_3209|Compound:9113616|FD-Score:-5.89|P-value:3.72E-7 ID:SGTC_3211|Compound:9128781|FD-Score:-7.68|P-value:5.42E-11 ID:SGTC_3234|Compound:9132280|FD-Score:-4.72|P-value:3.64E-5 ID:SGTC_3247|Compound:9135149|FD-Score:7.92|P-value:1.56E-11 ID:SGTC_3266|Compound:9136860|FD-Score:4.40|P-value:1.14E-4 ID:SGTC_3311|Compound:9131146|FD-Score:5.46|P-value:2.35E-6 ID:SGTC_17|Compound:0960-0276|FD-Score:4.14|P-value:2.63E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:3.86|P-value:6.03E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.83|P-value:2.58E-11 ID:SGTC_110|Compound:0850-0284|FD-Score:-4.90|P-value:1.86E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:4.29|P-value:1.63E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:22.70|P-value:3.39E-81 ID:SGTC_141|Compound:0443-0274|FD-Score:6.10|P-value:1.59E-7 ID:SGTC_157|Compound:1348-1695|FD-Score:3.74|P-value:8.61E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.03|P-value:3.72E-4 ID:SGTC_319|Compound:1348-1596|FD-Score:3.76|P-value:8.12E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.55|P-value:6.75E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:6.28|P-value:6.97E-8 ID:SGTC_586|Compound:0957-0265|FD-Score:5.46|P-value:2.36E-6 ID:SGTC_673|Compound:1082-0474|FD-Score:10.60|P-value:2.13E-19 ID:SGTC_709|Compound:3807-1509|FD-Score:3.91|P-value:5.19E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:4.49|P-value:8.40E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:4.20|P-value:2.16E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:6.03|P-value:2.17E-7 ID:SGTC_982|Compound:1491-0518|FD-Score:3.90|P-value:5.42E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.73|P-value:3.72E-5 ID:SGTC_999|Compound:4022-0066|FD-Score:5.52|P-value:1.86E-6 ID:SGTC_1002|Compound:4049-0206|FD-Score:6.09|P-value:1.66E-7 ID:SGTC_1050|Compound:3232-1156|FD-Score:6.58|P-value:1.68E-8 ID:SGTC_1094|Compound:duloxetine|FD-Score:-4.35|P-value:1.25E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:4.79|P-value:2.95E-5 ID:SGTC_1139|Compound:k015-0024|FD-Score:-4.91|P-value:1.84E-5 ID:SGTC_1140|Compound:3229-1848|FD-Score:5.07|P-value:1.06E-5 ID:SGTC_1143|Compound:3454-2408|FD-Score:-4.61|P-value:5.26E-5 ID:SGTC_1146|Compound:3579-1023|FD-Score:-4.18|P-value:2.15E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:8.34|P-value:1.33E-12 ID:SGTC_1163|Compound:k060-0048|FD-Score:6.62|P-value:1.39E-8 ID:SGTC_1195|Compound:1502-0147|FD-Score:4.37|P-value:1.23E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:-4.50|P-value:7.71E-5 ID:SGTC_1257|Compound:0669-0121|FD-Score:-4.42|P-value:1.01E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:-4.65|P-value:4.63E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.89|P-value:5.52E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:4.52|P-value:7.69E-5 ID:SGTC_1264|Compound:0783-0040|FD-Score:-6.94|P-value:2.76E-9 ID:SGTC_1351|Compound:1496-0003|FD-Score:4.09|P-value:3.02E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:3.76|P-value:8.05E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.39|P-value:1.16E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.79|P-value:7.58E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:-3.69|P-value:9.49E-4 ID:SGTC_1694|Compound:st024011|FD-Score:4.42|P-value:1.05E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.90|P-value:5.39E-4 ID:SGTC_1707|Compound:st032323|FD-Score:5.17|P-value:7.31E-6 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.30|P-value:1.58E-4 ID:SGTC_1915|Compound:st067105|FD-Score:5.18|P-value:6.92E-6 ID:SGTC_1930|Compound:st074634|FD-Score:4.19|P-value:2.21E-4 ID:SGTC_1964|Compound:st076914|FD-Score:3.94|P-value:4.76E-4 ID:SGTC_2026|Compound:5148700|FD-Score:6.39|P-value:4.16E-8 ID:SGTC_2043|Compound:5101342|FD-Score:4.31|P-value:1.50E-4 ID:SGTC_2264|Compound:7948757|FD-Score:3.83|P-value:6.60E-4 ID:SGTC_2289|Compound:7946404|FD-Score:6.10|P-value:1.57E-7 ID:SGTC_2290|Compound:7904846|FD-Score:4.15|P-value:2.49E-4 ID:SGTC_2340|Compound:7987427|FD-Score:-4.36|P-value:1.22E-4 ID:SGTC_2361|Compound:9034121|FD-Score:4.24|P-value:1.93E-4 ID:SGTC_2376|Compound:9072922|FD-Score:3.99|P-value:4.15E-4 ID:SGTC_2396|Compound:5342950|FD-Score:4.29|P-value:1.62E-4 ID:SGTC_2451|Compound:5856223|FD-Score:4.26|P-value:1.80E-4 ID:SGTC_2479|Compound:5617353|FD-Score:4.32|P-value:1.49E-4 ID:SGTC_2487|Compound:5918373|FD-Score:-4.49|P-value:8.03E-5 ID:SGTC_2495|Compound:cafestol|FD-Score:4.77|P-value:3.14E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.73|P-value:8.82E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:5.82|P-value:5.23E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:11.30|P-value:1.76E-21 ID:SGTC_2645|Compound:abamectin|FD-Score:-3.86|P-value:5.85E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:4.34|P-value:1.37E-4 ID:SGTC_2827|Compound:7993847|FD-Score:4.48|P-value:8.59E-5 ID:SGTC_2845|Compound:9003024|FD-Score:4.01|P-value:3.90E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.53|P-value:6.83E-5 ID:SGTC_2938|Compound:9059746|FD-Score:-4.37|P-value:1.20E-4 ID:SGTC_3013|Compound:9080133|FD-Score:-4.15|P-value:2.39E-4 ID:SGTC_3044|Compound:9093193|FD-Score:-4.92|P-value:1.76E-5 ID:SGTC_3049|Compound:9092223|FD-Score:3.96|P-value:4.51E-4 ID:SGTC_3052|Compound:9092717|FD-Score:5.81|P-value:5.62E-7 ID:SGTC_3203|Compound:9113543|FD-Score:-3.80|P-value:6.98E-4 ID:SGTC_3208|Compound:9114115|FD-Score:5.62|P-value:1.24E-6 ID:SGTC_3209|Compound:9113616|FD-Score:-5.89|P-value:3.72E-7 ID:SGTC_3211|Compound:9128781|FD-Score:-7.68|P-value:5.42E-11 ID:SGTC_3234|Compound:9132280|FD-Score:-4.72|P-value:3.64E-5 ID:SGTC_3247|Compound:9135149|FD-Score:7.92|P-value:1.56E-11 ID:SGTC_3266|Compound:9136860|FD-Score:4.40|P-value:1.14E-4 ID:SGTC_3311|Compound:9131146|FD-Score:5.46|P-value:2.35E-6

Top fitness defect scores for YDR540C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_138 0850-0259 22.70 40.9 µM 3.39E-81 cell wall 4 2.58E-114
2 SGTC_2619 obliquin 11.30 87.9 µM 1.76E-21 cell wall 15 1.05E-29
3 SGTC_673 1082-0474 10.60 110.0 µM 2.13E-19 RSC complex & mRNA processing 8 9.33E-27
4 SGTC_1162 k048-0130 8.34 20.1 µM 1.33E-12 Golgi 5 3.77E-17
5 SGTC_3247 9135149 7.92 49.5 µM 1.56E-11 DNA damage response 6 1.21E-15
6 SGTC_58 0957-0393 7.83 9.0 µM 2.58E-11 cell wall 132 2.47E-15
7 SGTC_1163 k060-0048 6.62 49.9 µM 1.39E-8 3 1.75E-11
8 SGTC_1050 3232-1156 6.58 69.3 µM 1.68E-8 RSC complex & mRNA processing 2 2.28E-11
9 SGTC_2026 5148700 6.39 152.0 µM 4.16E-8 3 8.20E-11
10 SGTC_514 4466-0038 6.28 2.6 nM 6.97E-8 cell wall 69 1.69E-10
11 SGTC_2289 7946404 6.10 165.1 µM 1.57E-7 35 5.29E-10
12 SGTC_141 0443-0274 6.10 32.3 µM 1.59E-7 mitochondrial response to ROS 16 5.39E-10
13 SGTC_1002 4049-0206 6.09 172.0 µM 1.66E-7 5 5.74E-10
14 SGTC_929 2805-0021 6.03 398.0 µM 2.17E-7 2 8.36E-10
15 SGTC_2507 purpurin 5.82 79.7 µM 5.23E-7 copper-dependent oxidative stress 14 2.88E-9
16 SGTC_3052 9092717 5.81 49.5 µM 5.62E-7 4 3.18E-9
17 SGTC_3208 9114115 5.62 49.5 µM 1.24E-6 14 9.67E-9
18 SGTC_999 4022-0066 5.52 22.7 µM 1.86E-6 Golgi 16 1.71E-8
19 SGTC_3311 9131146 5.46 2.9 µM 2.35E-6 cell wall 73 2.37E-8
20 SGTC_586 0957-0265 5.46 8.2 µM 2.36E-6 cell wall 22 2.39E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.316 1.91E-78 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.303 3.57E-72 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.294 1.01E-67 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.290 7.11E-66 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.278 8.51E-61 YOR051C ETT1 hom Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.276 1.45E-59 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.276 1.53E-59 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.272 4.55E-58 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.270 2.55E-57 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.266 2.93E-55 YLL021W SPA2 hom Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.264 1.00E-54 YJL073W JEM1 hom DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
0.259 1.28E-52 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.256 2.00E-51 YHR207C SET5 hom Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
0.256 2.90E-51 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.254 1.11E-50 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria