YEL002C / WBP1

Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum

Zygosity: Heterozygous strain
fixedexpanded
Profile for YEL002C / WBP1

Click on Significant Values for Screen Details ID:SGTC_70|Compound:0141-0289|FD-Score:-4.10|P-value:2.94E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.14|P-value:2.37E-7 ID:SGTC_158|Compound:k038-0087|FD-Score:3.58|P-value:2.26E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:3.73|P-value:1.32E-4 ID:SGTC_326|Compound:k292-0812|FD-Score:-3.47|P-value:3.34E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:-3.80|P-value:9.93E-5 ID:SGTC_346|Compound:4513-0042|FD-Score:3.49|P-value:3.18E-4 ID:SGTC_643|Compound:k048-0106|FD-Score:3.44|P-value:3.88E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:-4.02|P-value:4.10E-5 ID:SGTC_659|Compound:k018-0002|FD-Score:4.97|P-value:5.88E-7 ID:SGTC_772|Compound:k072-0172|FD-Score:-4.03|P-value:3.87E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.80|P-value:9.88E-5 ID:SGTC_924|Compound:2046-0044|FD-Score:3.48|P-value:3.31E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:5.26|P-value:1.33E-7 ID:SGTC_1275|Compound:0867-0008|FD-Score:4.46|P-value:6.32E-6 ID:SGTC_1432|Compound:4049-0298|FD-Score:3.39|P-value:4.50E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:-3.93|P-value:5.80E-5 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.55|P-value:2.60E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:-3.49|P-value:3.12E-4 ID:SGTC_1626|Compound:st003712|FD-Score:3.30|P-value:6.13E-4 ID:SGTC_1641|Compound:st009299|FD-Score:-3.82|P-value:8.95E-5 ID:SGTC_1780|Compound:st038448|FD-Score:5.13|P-value:2.54E-7 ID:SGTC_1784|Compound:st039334|FD-Score:3.60|P-value:2.15E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.40|P-value:8.49E-6 ID:SGTC_1847|Compound:st056231|FD-Score:3.40|P-value:4.39E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-3.28|P-value:6.51E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-5.09|P-value:3.03E-7 ID:SGTC_2108|Compound:5365811|FD-Score:-4.01|P-value:4.28E-5 ID:SGTC_2273|Compound:7815444|FD-Score:3.32|P-value:5.83E-4 ID:SGTC_2299|Compound:7976368|FD-Score:6.40|P-value:1.86E-10 ID:SGTC_2302|Compound:7771753|FD-Score:5.86|P-value:4.83E-9 ID:SGTC_2303|Compound:7774051|FD-Score:4.26|P-value:1.56E-5 ID:SGTC_2363|Compound:9053361|FD-Score:3.17|P-value:9.64E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:5.04|P-value:3.99E-7 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.81|P-value:9.78E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.75|P-value:1.21E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-6.97|P-value:4.42E-12 ID:SGTC_3234|Compound:9132280|FD-Score:5.20|P-value:1.84E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:-4.10|P-value:2.94E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.14|P-value:2.37E-7 ID:SGTC_158|Compound:k038-0087|FD-Score:3.58|P-value:2.26E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:3.73|P-value:1.32E-4 ID:SGTC_326|Compound:k292-0812|FD-Score:-3.47|P-value:3.34E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:-3.80|P-value:9.93E-5 ID:SGTC_346|Compound:4513-0042|FD-Score:3.49|P-value:3.18E-4 ID:SGTC_643|Compound:k048-0106|FD-Score:3.44|P-value:3.88E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:-4.02|P-value:4.10E-5 ID:SGTC_659|Compound:k018-0002|FD-Score:4.97|P-value:5.88E-7 ID:SGTC_772|Compound:k072-0172|FD-Score:-4.03|P-value:3.87E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.80|P-value:9.88E-5 ID:SGTC_924|Compound:2046-0044|FD-Score:3.48|P-value:3.31E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:5.26|P-value:1.33E-7 ID:SGTC_1275|Compound:0867-0008|FD-Score:4.46|P-value:6.32E-6 ID:SGTC_1432|Compound:4049-0298|FD-Score:3.39|P-value:4.50E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:-3.93|P-value:5.80E-5 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.55|P-value:2.60E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:-3.49|P-value:3.12E-4 ID:SGTC_1626|Compound:st003712|FD-Score:3.30|P-value:6.13E-4 ID:SGTC_1641|Compound:st009299|FD-Score:-3.82|P-value:8.95E-5 ID:SGTC_1780|Compound:st038448|FD-Score:5.13|P-value:2.54E-7 ID:SGTC_1784|Compound:st039334|FD-Score:3.60|P-value:2.15E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.40|P-value:8.49E-6 ID:SGTC_1847|Compound:st056231|FD-Score:3.40|P-value:4.39E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-3.28|P-value:6.51E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-5.09|P-value:3.03E-7 ID:SGTC_2108|Compound:5365811|FD-Score:-4.01|P-value:4.28E-5 ID:SGTC_2273|Compound:7815444|FD-Score:3.32|P-value:5.83E-4 ID:SGTC_2299|Compound:7976368|FD-Score:6.40|P-value:1.86E-10 ID:SGTC_2302|Compound:7771753|FD-Score:5.86|P-value:4.83E-9 ID:SGTC_2303|Compound:7774051|FD-Score:4.26|P-value:1.56E-5 ID:SGTC_2363|Compound:9053361|FD-Score:3.17|P-value:9.64E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:5.04|P-value:3.99E-7 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.81|P-value:9.78E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.75|P-value:1.21E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-6.97|P-value:4.42E-12 ID:SGTC_3234|Compound:9132280|FD-Score:5.20|P-value:1.84E-7

Top fitness defect scores for YEL002C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2299 7976368 6.40 114.9 µM 1.86E-10 1 7.71E-11 1.41
2 SGTC_2302 7771753 5.86 200.0 µM 4.83E-9 3 2.29E-9 1.30
3 SGTC_1020 4335-0961 5.26 93.3 µM 1.33E-7 2 7.24E-8 0.36
4 SGTC_3234 9132280 5.20 49.5 µM 1.84E-7 1 1.02E-7 0.67
5 SGTC_1780 st038448 5.13 24.5 µM 2.54E-7 iron homeostasis 2 1.42E-7 0.49
6 SGTC_2564 betulinic acid 5.04 100.0 µM 3.99E-7 5 2.28E-7 0.05
7 SGTC_659 k018-0002 4.97 107.0 µM 5.88E-7 3 3.42E-7 0.51
8 SGTC_1275 0867-0008 4.46 21.1 µM 6.32E-6 2 4.06E-6 0.30
9 SGTC_1800 st050079 4.40 29.1 µM 8.49E-6 NEO1-PIK1 19 5.52E-6 0.05
10 SGTC_2303 7774051 4.26 200.0 µM 1.56E-5 2 1.04E-5 0.06
11 SGTC_2580 diallyl disulphide 3.81 81.4 µM 9.78E-5 6 7.04E-5 0.05
12 SGTC_318 1488-0629 3.73 67.2 µM 1.32E-4 copper-dependent oxidative stress 3 9.64E-5 0.45
13 SGTC_1784 st039334 3.60 36.3 µM 2.15E-4 13 1.60E-4 0.11
14 SGTC_158 k038-0087 3.58 26.4 µM 2.26E-4 ubiquinone biosynthesis & proteasome 36 1.69E-4 0.10
15 SGTC_1532 4',5'-dihydroxyflavone 3.55 78.7 µM 2.60E-4 Golgi 12 1.95E-4 0.12
16 SGTC_346 4513-0042 3.49 7.2 µM 3.18E-4 azole & statin 11 2.41E-4 0.08
17 SGTC_924 2046-0044 3.48 21.3 µM 3.31E-4 10 2.50E-4 0.04
18 SGTC_643 k048-0106 3.44 28.4 µM 3.88E-4 RSC & ERG11 19 2.95E-4 0.22
19 SGTC_1847 st056231 3.40 15.0 µM 4.39E-4 8 3.36E-4 0.18
20 SGTC_1432 4049-0298 3.39 68.4 µM 4.50E-4 4 3.44E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.159 1.91E-20 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.127 1.37E-13 YER159C BUR6 het Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
0.118 7.31E-12 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.109 2.55E-10 YOR304W ISW2 hom ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth
0.106 6.39E-10 YBR070C ALG14 het Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
0.100 7.56E-9 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.095 3.47E-8 YLR135W SLX4 hom Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
0.095 3.74E-8 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.094 4.44E-8 YCR088W ABP1 hom Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences
0.094 5.66E-8 YJL072C PSF2 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.092 8.60E-8 YER136W GDI1 het GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
0.091 1.15E-7 YJL143W TIM17 het Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
0.089 2.19E-7 YHR007C ERG11 het Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
0.087 4.57E-7 YJR131W MNS1 hom Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation
0.087 5.08E-7 YIL063C YRB2 het Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential