HIPHOP chemogenomics database

YEL005C / VAB2

Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter

Zygosity: Homozygous strain

fixedexpanded
Profile for YEL005C / VAB2

Click on Significant Values for Screen Details

Top fitness defect scores for YEL005C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2688	kanamycin b	9.66	96.7 µM	7.08E-23	calcium & mitochondrial duress	31	2.14E-22
2	SGTC_877	0771-0151	7.10	33.1 µM	3.48E-13		1	6.32E-13
3	SGTC_2977	9011197	6.91	71.4 µM	1.40E-12		3	2.46E-12
4	SGTC_2777	climbazole	6.28	1.5 µM	1.04E-10	iron homeostasis	9	1.66E-10
5	SGTC_922	1866-0035	5.40	480.0 µM	2.33E-8	excess fatty acid	27	3.29E-8
6	SGTC_1712	st032288	5.24	53.4 µM	5.88E-8	calcium & mitochondrial duress	104	8.14E-8
7	SGTC_3156	9099931	4.45	49.5 µM	3.38E-6		4	4.27E-6
8	SGTC_308	4092-1169	4.45	104.0 µM	3.47E-6	plasma membrane duress	36	4.39E-6
9	SGTC_2185	6113692	4.42	200.0 µM	3.90E-6	ergosterol biosynthesis	7	4.92E-6
10	SGTC_996	3970-0799	4.37	1.8 µM	5.02E-6		69	6.30E-6
11	SGTC_3070	9117005	4.12	49.5 µM	1.56E-5		3	1.91E-5
12	SGTC_606	k015-0008	4.09	38.6 µM	1.79E-5		11	2.18E-5
13	SGTC_528	3492-0019	4.05	172.0 µM	2.12E-5	calcium & mitochondrial duress	212	2.58E-5
14	SGTC_1935	st074532	4.02	41.6 µM	2.44E-5		29	2.96E-5
15	SGTC_400	oxethazaine	3.75	37.5 µM	7.47E-5	calcium & mitochondrial duress	25	8.84E-5
16	SGTC_1153	4055-0057	3.69	267.0 µM	9.48E-5		35	1.12E-4
17	SGTC_3037	9091026	3.65	49.5 µM	1.11E-4	NEO1-PIK1	142	1.30E-4
18	SGTC_1937	st074714	3.64	47.2 µM	1.16E-4		50	1.36E-4
19	SGTC_1919	st067113	3.56	52.3 µM	1.58E-4	TSC3-RPN4	43	1.84E-4
20	SGTC_1665	st014639	3.48	11.4 µM	2.16E-4	NEO1	37	2.50E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.214	5.81E-36	YDR466W	PKH3	hom	Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.195	4.59E-30	YDL019C	OSH2	hom	Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.192	2.54E-29	YDR475C	JIP4	hom	Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.185	3.61E-27	YDR467C_d	YDR467C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.180	6.12E-26	YBR287W_p	YBR287W_p	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
0.180	7.36E-26	YKL061W_p	BLI1_p	hom	Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.178	3.66E-25	YDR101C	ARX1	hom	Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.167	2.06E-22	YKL051W	SFK1	hom	Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.159	2.21E-20	YGR237C_p	YGR237C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.157	5.19E-20	YMR099C	YMR099C	hom	Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
0.157	6.34E-20	YOR086C	TCB1	hom	Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.157	6.88E-20	YHL002W	HSE1	hom	Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
0.157	7.41E-20	YBL051C	PIN4	hom	Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.155	2.15E-19	YJL164C	TPK1	hom	cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.148	7.68E-18	YJL094C	KHA1	hom	Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies