YEL011W / GLC3

Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL011W / GLC3

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:3.06|P-value:5.92E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:2.98|P-value:8.11E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.26|P-value:2.84E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.52|P-value:9.95E-5 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.41|P-value:1.57E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.67|P-value:5.23E-5 ID:SGTC_1243|Compound:0416-0003|FD-Score:2.97|P-value:8.23E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:3.78|P-value:3.25E-5 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.36|P-value:1.90E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.18|P-value:3.80E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.51|P-value:1.56E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:3.82|P-value:2.79E-5 ID:SGTC_1606|Compound:st000094|FD-Score:3.09|P-value:5.38E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.22|P-value:3.27E-4 ID:SGTC_1828|Compound:st055811|FD-Score:2.92|P-value:9.89E-4 ID:SGTC_1967|Compound:st070177|FD-Score:3.69|P-value:4.93E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-3.55|P-value:1.31E-4 ID:SGTC_2099|Compound:5377090|FD-Score:3.32|P-value:2.24E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.41|P-value:1.57E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.40|P-value:2.40E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.83|P-value:2.70E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:4.16|P-value:5.76E-6 ID:SGTC_2525|Compound:harmine|FD-Score:3.63|P-value:6.31E-5 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.03|P-value:9.55E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.19|P-value:5.05E-6 ID:SGTC_2983|Compound:9022671|FD-Score:3.09|P-value:5.30E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-4.23|P-value:6.68E-6 ID:SGTC_56|Compound:0957-0365|FD-Score:3.06|P-value:5.92E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:2.98|P-value:8.11E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.26|P-value:2.84E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.52|P-value:9.95E-5 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.41|P-value:1.57E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.67|P-value:5.23E-5 ID:SGTC_1243|Compound:0416-0003|FD-Score:2.97|P-value:8.23E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:3.78|P-value:3.25E-5 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.36|P-value:1.90E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.18|P-value:3.80E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.51|P-value:1.56E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:3.82|P-value:2.79E-5 ID:SGTC_1606|Compound:st000094|FD-Score:3.09|P-value:5.38E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.22|P-value:3.27E-4 ID:SGTC_1828|Compound:st055811|FD-Score:2.92|P-value:9.89E-4 ID:SGTC_1967|Compound:st070177|FD-Score:3.69|P-value:4.93E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-3.55|P-value:1.31E-4 ID:SGTC_2099|Compound:5377090|FD-Score:3.32|P-value:2.24E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.41|P-value:1.57E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.40|P-value:2.40E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.83|P-value:2.70E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:4.16|P-value:5.76E-6 ID:SGTC_2525|Compound:harmine|FD-Score:3.63|P-value:6.31E-5 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.03|P-value:9.55E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.19|P-value:5.05E-6 ID:SGTC_2983|Compound:9022671|FD-Score:3.09|P-value:5.30E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-4.23|P-value:6.68E-6

Top fitness defect scores for YEL011W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2640 pentamidine 4.19 10.4 µM 5.05E-6 mitochondrial processes 27 1.40E-5
2 SGTC_2522 biochanin a 4.16 4.9 µM 5.76E-6 RNA pol III & RNase P/MRP 9 1.58E-5
3 SGTC_2520 obtusaquinone 3.83 4.7 µM 2.70E-5 22 6.50E-5
4 SGTC_1467 k284-1082 3.82 293.0 µM 2.79E-5 20 6.70E-5
5 SGTC_1280 0880-0253 3.78 10.1 µM 3.25E-5 22 7.72E-5
6 SGTC_1967 st070177 3.69 51.4 µM 4.93E-5 19 1.13E-4
7 SGTC_1194 1488-0194 3.67 225.0 µM 5.23E-5 39 1.19E-4
8 SGTC_2525 harmine 3.63 47.9 µM 6.31E-5 ergosterol biosynthesis 14 1.41E-4
9 SGTC_234 nsc-17383 3.52 10.7 µM 9.95E-5 ERG2 32 2.14E-4
10 SGTC_2330 7979373 3.41 200.0 µM 1.57E-4 60S ribosome export 48 3.25E-4
11 SGTC_1178 0988-0033 3.41 191.0 µM 1.57E-4 50 3.26E-4
12 SGTC_1281 0884-0031 3.36 11.8 µM 1.90E-4 fatty acid desaturase (OLE1) 44 3.87E-4
13 SGTC_2099 5377090 3.32 177.4 µM 2.24E-4 48 4.50E-4
14 SGTC_219 mitoxanthrone 3.26 164.4 µM 2.84E-4 DNA intercalators 71 5.60E-4
15 SGTC_1611 st001856 3.22 99.4 µM 3.27E-4 47 6.36E-4
16 SGTC_1350 1495-0454 3.18 146.0 µM 3.80E-4 63 7.30E-4
17 SGTC_2983 9022671 3.09 13.0 µM 5.30E-4 60S ribosome export 36 9.90E-4
18 SGTC_1606 st000094 3.09 49.2 µM 5.38E-4 60S ribosome export 50 0.00100
19 SGTC_56 0957-0365 3.06 83.2 µM 5.92E-4 Golgi 91 0.00109
20 SGTC_184 1494-0393 2.98 24.7 µM 8.11E-4 115 0.00146

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.129 6.33E-14 YER105C NUP157 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication
0.121 2.29E-12 YHR020W YHR020W het Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
0.110 1.97E-10 YMR157C AIM36 hom Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies
0.107 5.10E-10 YCR017C CWH43 hom Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion
0.106 7.53E-10 YAL043C PTA1 het Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII
0.101 5.37E-9 YDR164C SEC1 het Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
0.098 1.13E-8 YDR113C PDS1 het Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
0.091 1.42E-7 YJR154W_p YJR154W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.090 1.99E-7 YPR004C AIM45 hom Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response
0.089 2.11E-7 YKL084W HOT13 hom Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc
0.087 4.66E-7 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.083 1.46E-6 YNL247W YNL247W het Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
0.081 2.75E-6 YDR406W PDR15 hom Plasma membrane ATP binding cassette (ABC) transporter, multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element
0.081 2.79E-6 YOL158C ENB1 hom Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment
0.080 3.32E-6 YPR003C_p YPR003C_p hom Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene