YEL012W / UBC8

Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL012W / UBC8

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:-4.33|P-value:8.15E-6 ID:SGTC_14|Compound:0354-0009|FD-Score:3.43|P-value:2.96E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-4.70|P-value:1.42E-6 ID:SGTC_67|Compound:0335-0847|FD-Score:5.38|P-value:3.69E-8 ID:SGTC_77|Compound:0828-0286|FD-Score:3.38|P-value:3.53E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:4.15|P-value:1.62E-5 ID:SGTC_188|Compound:3229-0966|FD-Score:3.24|P-value:5.78E-4 ID:SGTC_298|Compound:1391-0729|FD-Score:3.23|P-value:6.09E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.25|P-value:6.02E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:3.41|P-value:3.17E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:-4.84|P-value:7.12E-7 ID:SGTC_367|Compound:0335-0881|FD-Score:6.08|P-value:5.96E-10 ID:SGTC_372|Compound:0155-0182|FD-Score:5.53|P-value:1.56E-8 ID:SGTC_395|Compound:anisomycin|FD-Score:-3.65|P-value:1.42E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:3.24|P-value:5.92E-4 ID:SGTC_417|Compound:st075190|FD-Score:3.58|P-value:1.69E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-3.46|P-value:2.88E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:3.88|P-value:5.08E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:5.57|P-value:1.22E-8 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.68|P-value:1.14E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:4.08|P-value:2.16E-5 ID:SGTC_551|Compound:k048-0028|FD-Score:4.50|P-value:3.32E-6 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.92|P-value:4.29E-5 ID:SGTC_803|Compound:4488-1585|FD-Score:4.76|P-value:9.24E-7 ID:SGTC_807|Compound:1315-0151|FD-Score:3.67|P-value:1.19E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:3.75|P-value:8.76E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.24|P-value:6.38E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.08|P-value:9.98E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.16|P-value:7.82E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.62|P-value:1.45E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:-5.05|P-value:2.43E-7 ID:SGTC_1421|Compound:4029-0249|FD-Score:3.16|P-value:7.74E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:4.64|P-value:1.70E-6 ID:SGTC_1574|Compound:spiramycin|FD-Score:4.89|P-value:5.04E-7 ID:SGTC_1600|Compound:coumarin 106|FD-Score:4.42|P-value:4.77E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-3.65|P-value:1.40E-4 ID:SGTC_1753|Compound:st043925|FD-Score:-3.60|P-value:1.67E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.25|P-value:5.57E-4 ID:SGTC_1866|Compound:st057638|FD-Score:3.28|P-value:5.07E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:-3.82|P-value:7.11E-5 ID:SGTC_1881|Compound:5652484|FD-Score:-3.29|P-value:5.38E-4 ID:SGTC_1983|Compound:st071813|FD-Score:3.47|P-value:2.55E-4 ID:SGTC_2151|Compound:5563267|FD-Score:3.64|P-value:1.31E-4 ID:SGTC_2167|Compound:5876490|FD-Score:6.58|P-value:2.23E-11 ID:SGTC_2263|Compound:7948595|FD-Score:3.70|P-value:1.07E-4 ID:SGTC_2269|Compound:7935389|FD-Score:3.83|P-value:6.23E-5 ID:SGTC_2288|Compound:7946289|FD-Score:3.49|P-value:2.39E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.24|P-value:6.32E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-5.04|P-value:2.57E-7 ID:SGTC_3203|Compound:9113543|FD-Score:-3.17|P-value:8.10E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-3.46|P-value:2.88E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:-4.33|P-value:8.15E-6 ID:SGTC_14|Compound:0354-0009|FD-Score:3.43|P-value:2.96E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-4.70|P-value:1.42E-6 ID:SGTC_67|Compound:0335-0847|FD-Score:5.38|P-value:3.69E-8 ID:SGTC_77|Compound:0828-0286|FD-Score:3.38|P-value:3.53E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:4.15|P-value:1.62E-5 ID:SGTC_188|Compound:3229-0966|FD-Score:3.24|P-value:5.78E-4 ID:SGTC_298|Compound:1391-0729|FD-Score:3.23|P-value:6.09E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.25|P-value:6.02E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:3.41|P-value:3.17E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:-4.84|P-value:7.12E-7 ID:SGTC_367|Compound:0335-0881|FD-Score:6.08|P-value:5.96E-10 ID:SGTC_372|Compound:0155-0182|FD-Score:5.53|P-value:1.56E-8 ID:SGTC_395|Compound:anisomycin|FD-Score:-3.65|P-value:1.42E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:3.24|P-value:5.92E-4 ID:SGTC_417|Compound:st075190|FD-Score:3.58|P-value:1.69E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-3.46|P-value:2.88E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:3.88|P-value:5.08E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:5.57|P-value:1.22E-8 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.68|P-value:1.14E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:4.08|P-value:2.16E-5 ID:SGTC_551|Compound:k048-0028|FD-Score:4.50|P-value:3.32E-6 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.92|P-value:4.29E-5 ID:SGTC_803|Compound:4488-1585|FD-Score:4.76|P-value:9.24E-7 ID:SGTC_807|Compound:1315-0151|FD-Score:3.67|P-value:1.19E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:3.75|P-value:8.76E-5 ID:SGTC_923|Compound:1900-2434|FD-Score:-3.24|P-value:6.38E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.08|P-value:9.98E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.16|P-value:7.82E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:3.62|P-value:1.45E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:-5.05|P-value:2.43E-7 ID:SGTC_1421|Compound:4029-0249|FD-Score:3.16|P-value:7.74E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:4.64|P-value:1.70E-6 ID:SGTC_1574|Compound:spiramycin|FD-Score:4.89|P-value:5.04E-7 ID:SGTC_1600|Compound:coumarin 106|FD-Score:4.42|P-value:4.77E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-3.65|P-value:1.40E-4 ID:SGTC_1753|Compound:st043925|FD-Score:-3.60|P-value:1.67E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.25|P-value:5.57E-4 ID:SGTC_1866|Compound:st057638|FD-Score:3.28|P-value:5.07E-4 ID:SGTC_1880|Compound:MG-132|FD-Score:-3.82|P-value:7.11E-5 ID:SGTC_1881|Compound:5652484|FD-Score:-3.29|P-value:5.38E-4 ID:SGTC_1983|Compound:st071813|FD-Score:3.47|P-value:2.55E-4 ID:SGTC_2151|Compound:5563267|FD-Score:3.64|P-value:1.31E-4 ID:SGTC_2167|Compound:5876490|FD-Score:6.58|P-value:2.23E-11 ID:SGTC_2263|Compound:7948595|FD-Score:3.70|P-value:1.07E-4 ID:SGTC_2269|Compound:7935389|FD-Score:3.83|P-value:6.23E-5 ID:SGTC_2288|Compound:7946289|FD-Score:3.49|P-value:2.39E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.24|P-value:6.32E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-5.04|P-value:2.57E-7 ID:SGTC_3203|Compound:9113543|FD-Score:-3.17|P-value:8.10E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-3.46|P-value:2.88E-4

Top fitness defect scores for YEL012W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2167 5876490 6.58 200.0 µM 2.23E-11 2 2.28E-11
2 SGTC_367 0335-0881 6.08 7.1 µM 5.96E-10 ubiquinone biosynthesis & proteasome 25 6.09E-10
3 SGTC_453 enantio-paf c16 5.57 10.0 µM 1.22E-8 65 1.25E-8
4 SGTC_372 0155-0182 5.53 15.9 µM 1.56E-8 mitochondrial response to ROS 8 1.60E-8
5 SGTC_67 0335-0847 5.38 266.0 µM 3.69E-8 ubiquinone biosynthesis & proteasome 78 3.78E-8
6 SGTC_1574 spiramycin 4.89 23.7 µM 5.04E-7 12 5.17E-7
7 SGTC_803 4488-1585 4.76 147.0 µM 9.24E-7 5 9.48E-7
8 SGTC_1535 tetrahydropapaveroline hydrobromide 4.64 54.3 µM 1.70E-6 RPP1 & pyrimidine depletion 2 1.75E-6
9 SGTC_551 k048-0028 4.50 34.9 µM 3.32E-6 6 3.41E-6
10 SGTC_1600 coumarin 106 4.42 71.1 µM 4.77E-6 19 4.90E-6
11 SGTC_127 0180-0333 4.15 59.1 µM 1.62E-5 mitochondrial response to ROS 37 1.66E-5
12 SGTC_543 3844-1095 4.08 191.0 µM 2.16E-5 ERG2 46 2.22E-5
13 SGTC_563 tetrahydrosecaminediol 3.92 614.0 µM 4.29E-5 amide catabolism 17 4.40E-5
14 SGTC_426 0988-0037 3.88 14.6 µM 5.08E-5 ERAD & cell cycle 35 5.22E-5
15 SGTC_2269 7935389 3.83 174.4 µM 6.23E-5 tubulin folding & SWR complex 30 6.40E-5
16 SGTC_857 0438-0306 3.75 1.4 µM 8.76E-5 copper-dependent oxidative stress 17 8.99E-5
17 SGTC_2263 7948595 3.70 193.3 µM 1.07E-4 11 1.10E-4
18 SGTC_457 grayanotoxin iii 3.68 135.0 µM 1.14E-4 88 1.17E-4
19 SGTC_807 1315-0151 3.67 48.0 µM 1.19E-4 37 1.22E-4
20 SGTC_2151 5563267 3.64 85.4 µM 1.31E-4 30 1.34E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.126 2.74E-13 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.123 8.88E-13 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.118 6.60E-12 YPL067C_p YPL067C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene
0.112 6.47E-11 YOL080C REX4 hom Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly
0.106 8.52E-10 YLR378C SEC61 het Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
0.105 1.13E-9 YDL002C NHP10 hom Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair
0.103 2.25E-9 YPL126W NAN1 het U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA
0.101 4.76E-9 YDR118W APC4 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
0.100 6.06E-9 YGR100W MDR1 hom Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function
0.100 6.94E-9 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.097 1.95E-8 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.095 3.38E-8 YPL246C RBD2 hom Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
0.095 3.61E-8 YMR135C GID8 hom Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START
0.092 8.87E-8 YBR169C SSE2 hom Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
0.089 2.37E-7 YLR214W FRE1 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels