YEL019C / MMS21

SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination

Zygosity: Heterozygous strain
fixedexpanded
Profile for YEL019C / MMS21

Click on Significant Values for Screen Details ID:SGTC_25|Compound:0118-0223|FD-Score:3.23|P-value:3.20E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.13|P-value:4.59E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.02|P-value:6.90E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-7.34|P-value:4.17E-15 ID:SGTC_462|Compound:ag-879|FD-Score:4.43|P-value:1.44E-6 ID:SGTC_639|Compound:0833-0042|FD-Score:3.31|P-value:2.33E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.22|P-value:3.29E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.02|P-value:6.83E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.34|P-value:2.06E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:4.04|P-value:9.75E-6 ID:SGTC_778|Compound:3456-2434|FD-Score:-6.42|P-value:5.69E-12 ID:SGTC_1064|Compound:honokiol|FD-Score:-2.94|P-value:9.38E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:4.67|P-value:3.92E-7 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.10|P-value:5.20E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:-3.05|P-value:6.31E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.52|P-value:9.79E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.06|P-value:6.21E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:-3.04|P-value:6.48E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.80|P-value:2.89E-5 ID:SGTC_1521|Compound:st012942|FD-Score:-3.15|P-value:4.24E-4 ID:SGTC_1742|Compound:st038235|FD-Score:2.98|P-value:8.16E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.52|P-value:1.02E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.27|P-value:2.80E-4 ID:SGTC_1971|Compound:st069429|FD-Score:3.43|P-value:1.45E-4 ID:SGTC_2140|Compound:5586273|FD-Score:-3.70|P-value:4.42E-5 ID:SGTC_2144|Compound:5628481|FD-Score:-4.18|P-value:4.94E-6 ID:SGTC_2320|Compound:7373003|FD-Score:2.94|P-value:9.63E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.37|P-value:1.86E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.14|P-value:4.52E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.48|P-value:1.17E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:-4.59|P-value:6.06E-7 ID:SGTC_2666|Compound:tannic acid|FD-Score:-4.38|P-value:1.80E-6 ID:SGTC_2710|Compound:st078562|FD-Score:3.13|P-value:4.76E-4 ID:SGTC_2712|Compound:st079126|FD-Score:3.86|P-value:2.22E-5 ID:SGTC_2713|Compound:st079149|FD-Score:-3.06|P-value:6.03E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:3.67|P-value:5.21E-5 ID:SGTC_2841|Compound:9004761|FD-Score:-3.39|P-value:1.65E-4 ID:SGTC_2845|Compound:9003024|FD-Score:3.91|P-value:1.78E-5 ID:SGTC_2951|Compound:9072950|FD-Score:-5.77|P-value:5.02E-10 ID:SGTC_2991|Compound:9074669|FD-Score:5.20|P-value:1.92E-8 ID:SGTC_3002|Compound:9075603|FD-Score:-4.35|P-value:2.03E-6 ID:SGTC_3106|Compound:9121286|FD-Score:-3.92|P-value:1.65E-5 ID:SGTC_3168|Compound:9103419|FD-Score:4.45|P-value:1.28E-6 ID:SGTC_3221|Compound:9128209|FD-Score:2.94|P-value:9.42E-4 ID:SGTC_3257|Compound:9139206|FD-Score:2.98|P-value:8.08E-4 ID:SGTC_3276|Compound:9139060|FD-Score:2.99|P-value:7.80E-4 ID:SGTC_25|Compound:0118-0223|FD-Score:3.23|P-value:3.20E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.13|P-value:4.59E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:-3.02|P-value:6.90E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-7.34|P-value:4.17E-15 ID:SGTC_462|Compound:ag-879|FD-Score:4.43|P-value:1.44E-6 ID:SGTC_639|Compound:0833-0042|FD-Score:3.31|P-value:2.33E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.22|P-value:3.29E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.02|P-value:6.83E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.34|P-value:2.06E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:4.04|P-value:9.75E-6 ID:SGTC_778|Compound:3456-2434|FD-Score:-6.42|P-value:5.69E-12 ID:SGTC_1064|Compound:honokiol|FD-Score:-2.94|P-value:9.38E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:4.67|P-value:3.92E-7 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.10|P-value:5.20E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:-3.05|P-value:6.31E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.52|P-value:9.79E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.06|P-value:6.21E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:-3.04|P-value:6.48E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.80|P-value:2.89E-5 ID:SGTC_1521|Compound:st012942|FD-Score:-3.15|P-value:4.24E-4 ID:SGTC_1742|Compound:st038235|FD-Score:2.98|P-value:8.16E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.52|P-value:1.02E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.27|P-value:2.80E-4 ID:SGTC_1971|Compound:st069429|FD-Score:3.43|P-value:1.45E-4 ID:SGTC_2140|Compound:5586273|FD-Score:-3.70|P-value:4.42E-5 ID:SGTC_2144|Compound:5628481|FD-Score:-4.18|P-value:4.94E-6 ID:SGTC_2320|Compound:7373003|FD-Score:2.94|P-value:9.63E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.37|P-value:1.86E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.14|P-value:4.52E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.48|P-value:1.17E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:-4.59|P-value:6.06E-7 ID:SGTC_2666|Compound:tannic acid|FD-Score:-4.38|P-value:1.80E-6 ID:SGTC_2710|Compound:st078562|FD-Score:3.13|P-value:4.76E-4 ID:SGTC_2712|Compound:st079126|FD-Score:3.86|P-value:2.22E-5 ID:SGTC_2713|Compound:st079149|FD-Score:-3.06|P-value:6.03E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:3.67|P-value:5.21E-5 ID:SGTC_2841|Compound:9004761|FD-Score:-3.39|P-value:1.65E-4 ID:SGTC_2845|Compound:9003024|FD-Score:3.91|P-value:1.78E-5 ID:SGTC_2951|Compound:9072950|FD-Score:-5.77|P-value:5.02E-10 ID:SGTC_2991|Compound:9074669|FD-Score:5.20|P-value:1.92E-8 ID:SGTC_3002|Compound:9075603|FD-Score:-4.35|P-value:2.03E-6 ID:SGTC_3106|Compound:9121286|FD-Score:-3.92|P-value:1.65E-5 ID:SGTC_3168|Compound:9103419|FD-Score:4.45|P-value:1.28E-6 ID:SGTC_3221|Compound:9128209|FD-Score:2.94|P-value:9.42E-4 ID:SGTC_3257|Compound:9139206|FD-Score:2.98|P-value:8.08E-4 ID:SGTC_3276|Compound:9139060|FD-Score:2.99|P-value:7.80E-4

Top fitness defect scores for YEL019C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2991 9074669 5.20 71.4 µM 1.92E-8 azole & statin 8 9.85E-8 0.02
2 SGTC_1080 nisoldipine 4.67 24.4 µM 3.92E-7 TSC3-RPN4 2 1.48E-6 0.10
3 SGTC_3168 9103419 4.45 49.5 µM 1.28E-6 60S ribosome export 3 4.28E-6 0.32
4 SGTC_462 ag-879 4.43 158.0 µM 1.44E-6 10 4.77E-6 0.22
5 SGTC_764 1951-2361 4.04 146.0 µM 9.75E-6 9 2.66E-5 0.02
6 SGTC_2845 9003024 3.91 43.0 µM 1.78E-5 amide catabolism 14 4.56E-5 0.08
7 SGTC_2712 st079126 3.86 47.7 µM 2.22E-5 3 5.58E-5 0.06
8 SGTC_2724 maprotiline 3.67 69.6 µM 5.21E-5 ERG2 8 1.20E-4 0.26
9 SGTC_1172 0370-0043 3.52 85.0 µM 9.79E-5 6 2.12E-4 0.10
10 SGTC_1746 st038117 3.52 42.0 µM 1.02E-4 8 2.20E-4 0.18
11 SGTC_1971 st069429 3.43 63.5 µM 1.45E-4 60S ribosome export 3 3.02E-4 0.02
12 SGTC_2401 5128683 3.37 200.0 µM 1.86E-4 17 3.77E-4 0.03
13 SGTC_759 3486-0358 3.34 89.3 µM 2.06E-4 12 4.14E-4 0.19
14 SGTC_639 0833-0042 3.31 114.0 µM 2.33E-4 10 4.62E-4 2.93E-4
15 SGTC_1883 5-benzylidenerhodanine 3.27 20.0 µM 2.80E-4 TRP & mitochondrial translation 11 5.46E-4 0.02
16 SGTC_25 0118-0223 3.23 35.7 µM 3.20E-4 6 6.15E-4 0.05
17 SGTC_649 1123-0311 3.22 367.0 µM 3.29E-4 endomembrane recycling 17 6.32E-4 0.02
18 SGTC_2616 abietic acid 3.14 100.0 µM 4.52E-4 10 8.40E-4 0.16
19 SGTC_2710 st078562 3.13 53.7 µM 4.76E-4 14 8.81E-4 0.00
20 SGTC_1087 pterostilbene 3.10 9.1 µM 5.20E-4 16 9.53E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.181 3.10E-26 YHR164C DNA2 het Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
0.173 5.18E-24 YLL011W SOF1 het Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
0.136 2.95E-15 YGR245C SDA1 het Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
0.127 1.30E-13 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.124 5.37E-13 YGL074C_d YGL074C_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
0.121 2.15E-12 YNL132W KRE33 het Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
0.121 2.26E-12 YLR129W DIP2 het Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex
0.115 2.29E-11 YKL153W_d YKL153W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
0.112 7.67E-11 YLR143W DPH6 hom Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene
0.107 5.04E-10 YIL083C CAB2 het Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
0.106 7.12E-10 YLR167W RPS31 het Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
0.104 1.33E-9 YCR054C CTR86 het Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
0.103 1.85E-9 YPL231W FAS2 het Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities
0.103 2.50E-9 YER127W LCP5 het Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
0.099 9.18E-9 YOL026C MIM1 het Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex