YEL022W / GEA2

Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL022W / GEA2

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:10.10|P-value:2.07E-17 ID:SGTC_43|Compound:0570-0105|FD-Score:7.41|P-value:3.41E-10 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.77|P-value:9.45E-7 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.29|P-value:2.03E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.90|P-value:6.65E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:5.20|P-value:7.14E-6 ID:SGTC_250|Compound:brefeldin a|FD-Score:3.95|P-value:4.85E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.74|P-value:8.81E-4 ID:SGTC_433|Compound:pimozide|FD-Score:-3.84|P-value:7.83E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-5.37|P-value:4.67E-6 ID:SGTC_577|Compound:r071-0003|FD-Score:4.40|P-value:1.19E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:5.23|P-value:6.36E-6 ID:SGTC_886|Compound:0866-0150|FD-Score:4.59|P-value:6.31E-5 ID:SGTC_999|Compound:4022-0066|FD-Score:7.16|P-value:1.21E-9 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.41|P-value:1.16E-4 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-4.74|P-value:4.71E-5 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:-3.87|P-value:7.24E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:8.11|P-value:7.26E-12 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.54|P-value:7.49E-5 ID:SGTC_1458|Compound:k015-0034|FD-Score:-4.33|P-value:1.83E-4 ID:SGTC_1478|Compound:0453-0656|FD-Score:3.81|P-value:7.29E-4 ID:SGTC_1485|Compound:3909-9022|FD-Score:7.06|P-value:1.98E-9 ID:SGTC_1489|Compound:4065-0444|FD-Score:5.30|P-value:4.87E-6 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.75|P-value:3.69E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.80|P-value:7.50E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:4.18|P-value:2.39E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.98|P-value:5.23E-4 ID:SGTC_1530|Compound:rhein|FD-Score:4.99|P-value:1.53E-5 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.89|P-value:5.65E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.07|P-value:3.31E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:5.25|P-value:5.95E-6 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.80|P-value:7.34E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:-4.09|P-value:3.74E-4 ID:SGTC_1701|Compound:st028638|FD-Score:4.07|P-value:3.30E-4 ID:SGTC_1712|Compound:st032288|FD-Score:9.54|P-value:9.89E-16 ID:SGTC_1748|Compound:st037455|FD-Score:4.79|P-value:3.11E-5 ID:SGTC_1801|Compound:st050089|FD-Score:5.00|P-value:1.48E-5 ID:SGTC_1859|Compound:st055998|FD-Score:-3.78|P-value:9.36E-4 ID:SGTC_1888|Compound:azatryptophan|FD-Score:-4.80|P-value:3.82E-5 ID:SGTC_2016|Compound:5121758|FD-Score:-4.05|P-value:4.23E-4 ID:SGTC_2021|Compound:4019055|FD-Score:11.00|P-value:2.08E-20 ID:SGTC_2073|Compound:5212005|FD-Score:3.84|P-value:6.52E-4 ID:SGTC_2242|Compound:6630449|FD-Score:4.76|P-value:3.56E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.46|P-value:9.86E-5 ID:SGTC_2331|Compound:7980721|FD-Score:4.90|P-value:2.16E-5 ID:SGTC_2336|Compound:9001819|FD-Score:4.74|P-value:3.75E-5 ID:SGTC_2337|Compound:9009472|FD-Score:-3.98|P-value:5.22E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-3.90|P-value:6.70E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.78|P-value:9.38E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.85|P-value:7.73E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:8.66|P-value:2.76E-13 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.02|P-value:3.84E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:16.70|P-value:3.40E-44 ID:SGTC_2691|Compound:st077356|FD-Score:-5.18|P-value:9.68E-6 ID:SGTC_2694|Compound:st077685|FD-Score:4.53|P-value:7.59E-5 ID:SGTC_2810|Compound:7959871|FD-Score:6.56|P-value:2.24E-8 ID:SGTC_2826|Compound:7992541|FD-Score:-4.73|P-value:4.77E-5 ID:SGTC_3086|Compound:9117350|FD-Score:3.85|P-value:6.36E-4 ID:SGTC_3152|Compound:9099253|FD-Score:11.40|P-value:1.47E-21 ID:SGTC_3202|Compound:9113761|FD-Score:5.15|P-value:8.48E-6 ID:SGTC_15|Compound:0266-0086|FD-Score:10.10|P-value:2.07E-17 ID:SGTC_43|Compound:0570-0105|FD-Score:7.41|P-value:3.41E-10 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.77|P-value:9.45E-7 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.29|P-value:2.03E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.90|P-value:6.65E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:5.20|P-value:7.14E-6 ID:SGTC_250|Compound:brefeldin a|FD-Score:3.95|P-value:4.85E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.74|P-value:8.81E-4 ID:SGTC_433|Compound:pimozide|FD-Score:-3.84|P-value:7.83E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:-5.37|P-value:4.67E-6 ID:SGTC_577|Compound:r071-0003|FD-Score:4.40|P-value:1.19E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:5.23|P-value:6.36E-6 ID:SGTC_886|Compound:0866-0150|FD-Score:4.59|P-value:6.31E-5 ID:SGTC_999|Compound:4022-0066|FD-Score:7.16|P-value:1.21E-9 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.41|P-value:1.16E-4 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-4.74|P-value:4.71E-5 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:-3.87|P-value:7.24E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:8.11|P-value:7.26E-12 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.54|P-value:7.49E-5 ID:SGTC_1458|Compound:k015-0034|FD-Score:-4.33|P-value:1.83E-4 ID:SGTC_1478|Compound:0453-0656|FD-Score:3.81|P-value:7.29E-4 ID:SGTC_1485|Compound:3909-9022|FD-Score:7.06|P-value:1.98E-9 ID:SGTC_1489|Compound:4065-0444|FD-Score:5.30|P-value:4.87E-6 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.75|P-value:3.69E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.80|P-value:7.50E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:4.18|P-value:2.39E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.98|P-value:5.23E-4 ID:SGTC_1530|Compound:rhein|FD-Score:4.99|P-value:1.53E-5 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.89|P-value:5.65E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.07|P-value:3.31E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:5.25|P-value:5.95E-6 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.80|P-value:7.34E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:-4.09|P-value:3.74E-4 ID:SGTC_1701|Compound:st028638|FD-Score:4.07|P-value:3.30E-4 ID:SGTC_1712|Compound:st032288|FD-Score:9.54|P-value:9.89E-16 ID:SGTC_1748|Compound:st037455|FD-Score:4.79|P-value:3.11E-5 ID:SGTC_1801|Compound:st050089|FD-Score:5.00|P-value:1.48E-5 ID:SGTC_1859|Compound:st055998|FD-Score:-3.78|P-value:9.36E-4 ID:SGTC_1888|Compound:azatryptophan|FD-Score:-4.80|P-value:3.82E-5 ID:SGTC_2016|Compound:5121758|FD-Score:-4.05|P-value:4.23E-4 ID:SGTC_2021|Compound:4019055|FD-Score:11.00|P-value:2.08E-20 ID:SGTC_2073|Compound:5212005|FD-Score:3.84|P-value:6.52E-4 ID:SGTC_2242|Compound:6630449|FD-Score:4.76|P-value:3.56E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.46|P-value:9.86E-5 ID:SGTC_2331|Compound:7980721|FD-Score:4.90|P-value:2.16E-5 ID:SGTC_2336|Compound:9001819|FD-Score:4.74|P-value:3.75E-5 ID:SGTC_2337|Compound:9009472|FD-Score:-3.98|P-value:5.22E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-3.90|P-value:6.70E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.78|P-value:9.38E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.85|P-value:7.73E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:8.66|P-value:2.76E-13 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.02|P-value:3.84E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:16.70|P-value:3.40E-44 ID:SGTC_2691|Compound:st077356|FD-Score:-5.18|P-value:9.68E-6 ID:SGTC_2694|Compound:st077685|FD-Score:4.53|P-value:7.59E-5 ID:SGTC_2810|Compound:7959871|FD-Score:6.56|P-value:2.24E-8 ID:SGTC_2826|Compound:7992541|FD-Score:-4.73|P-value:4.77E-5 ID:SGTC_3086|Compound:9117350|FD-Score:3.85|P-value:6.36E-4 ID:SGTC_3152|Compound:9099253|FD-Score:11.40|P-value:1.47E-21 ID:SGTC_3202|Compound:9113761|FD-Score:5.15|P-value:8.48E-6

Top fitness defect scores for YEL022W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2688 kanamycin b 16.70 96.7 µM 3.40E-44 calcium & mitochondrial duress 5 5.07E-63
2 SGTC_3152 9099253 11.40 49.5 µM 1.47E-21 NEO1-PIK1 17 2.75E-30
3 SGTC_2021 4019055 11.00 173.0 µM 2.08E-20 endomembrane recycling 5 1.26E-28
4 SGTC_15 0266-0086 10.10 27.1 µM 2.07E-17 endomembrane recycling 5 2.67E-24
5 SGTC_1712 st032288 9.54 53.4 µM 9.89E-16 calcium & mitochondrial duress 29 7.02E-22
6 SGTC_2609 2,3,4-trihydroxy-4'-ethoxybenzophenone 8.66 70.0 µM 2.76E-13 redox potentiating 13 2.35E-18
7 SGTC_1445 4239-0156 8.11 97.7 µM 7.26E-12 1 2.58E-16
8 SGTC_43 0570-0105 7.41 72.5 µM 3.41E-10 ERG2 19 6.53E-14
9 SGTC_999 4022-0066 7.16 22.7 µM 1.21E-9 Golgi 9 4.01E-13
10 SGTC_1485 3909-9022 7.06 254.0 µM 1.98E-9 RPP1 & pyrimidine depletion 1 8.18E-13
11 SGTC_2810 7959871 6.56 45.5 µM 2.24E-8 copper-dependent oxidative stress 8 2.65E-11
12 SGTC_1489 4065-0444 5.30 182.0 µM 4.87E-6 6 5.81E-8
13 SGTC_1550 gossypolone 5.25 36.6 µM 5.95E-6 3 7.72E-8
14 SGTC_768 0384-0033 5.23 77.3 µM 6.36E-6 fatty acid desaturase (OLE1) 49 8.49E-8
15 SGTC_206 4546-0025 5.20 72.0 µM 7.14E-6 NEO1 37 1.00E-7
16 SGTC_3202 9113761 5.15 49.5 µM 8.48E-6 20 1.28E-7
17 SGTC_1801 st050089 5.00 28.4 µM 1.48E-5 NEO1-PIK1 74 2.81E-7
18 SGTC_1530 rhein 4.99 70.4 µM 1.53E-5 7 2.97E-7
19 SGTC_2331 7980721 4.90 200.0 µM 2.16E-5 6 4.85E-7
20 SGTC_1748 st037455 4.79 53.6 µM 3.11E-5 13 8.14E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.292 4.66E-67 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.256 3.13E-51 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.241 1.32E-45 YJL094C KHA1 hom Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
0.239 1.20E-44 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.236 6.83E-44 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.227 1.46E-40 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.226 2.92E-40 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.224 2.02E-39 YPL120W VPS30 hom Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.221 2.13E-38 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.213 7.59E-36 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.204 5.84E-33 YGL045W RIM8 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
0.202 2.36E-32 YLR408C_p BLS1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
0.202 4.59E-32 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.200 9.33E-32 YBL022C PIM1 hom ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
0.200 1.16E-31 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex