YEL027W / VMA3

Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL027W / VMA3

Click on Significant Values for Screen Details ID:SGTC_33|Compound:0986-0033|FD-Score:-3.34|P-value:8.56E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:4.61|P-value:8.49E-6 ID:SGTC_65|Compound:0798-0905|FD-Score:-4.94|P-value:1.77E-6 ID:SGTC_100|Compound:0086-0128|FD-Score:4.17|P-value:4.92E-5 ID:SGTC_180|Compound:1486-1293|FD-Score:6.17|P-value:4.02E-9 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.43|P-value:6.37E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:-5.62|P-value:6.68E-8 ID:SGTC_595|Compound:3909-8003|FD-Score:3.68|P-value:3.01E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:5.41|P-value:2.17E-7 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.50|P-value:5.52E-4 ID:SGTC_657|Compound:3534-0529|FD-Score:-3.44|P-value:6.08E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:4.29|P-value:3.11E-5 ID:SGTC_815|Compound:1582-0027|FD-Score:-4.21|P-value:3.84E-5 ID:SGTC_881|Compound:0833-0569|FD-Score:3.63|P-value:3.51E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-4.74|P-value:4.37E-6 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.62|P-value:8.00E-6 ID:SGTC_1046|Compound:2001-0062|FD-Score:3.71|P-value:2.68E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.82|P-value:1.82E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.38|P-value:8.13E-4 ID:SGTC_1319|Compound:1306-0155|FD-Score:-3.37|P-value:7.79E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:-3.64|P-value:3.15E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:5.90|P-value:1.74E-8 ID:SGTC_1634|Compound:st006576|FD-Score:-4.37|P-value:2.02E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.75|P-value:4.19E-6 ID:SGTC_1719|Compound:st032227|FD-Score:-4.31|P-value:2.57E-5 ID:SGTC_1900|Compound:st060200|FD-Score:3.36|P-value:8.53E-4 ID:SGTC_1959|Compound:st077091|FD-Score:5.16|P-value:7.23E-7 ID:SGTC_2001|Compound:4023502|FD-Score:4.54|P-value:1.13E-5 ID:SGTC_2005|Compound:4024620|FD-Score:3.48|P-value:5.78E-4 ID:SGTC_2020|Compound:4017018|FD-Score:-4.72|P-value:4.77E-6 ID:SGTC_2256|Compound:7954252|FD-Score:-4.86|P-value:2.53E-6 ID:SGTC_2302|Compound:7771753|FD-Score:5.11|P-value:8.93E-7 ID:SGTC_2348|Compound:9022543|FD-Score:3.44|P-value:6.62E-4 ID:SGTC_2351|Compound:9026858|FD-Score:5.14|P-value:7.67E-7 ID:SGTC_2395|Compound:5646831|FD-Score:3.66|P-value:3.15E-4 ID:SGTC_2462|Compound:5162487|FD-Score:3.84|P-value:1.66E-4 ID:SGTC_2470|Compound:5664817|FD-Score:3.37|P-value:8.38E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.93|P-value:1.21E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-3.71|P-value:2.44E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-5.60|P-value:7.30E-8 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:6.04|P-value:8.18E-9 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.52|P-value:1.23E-7 ID:SGTC_2696|Compound:st077691|FD-Score:-4.24|P-value:3.45E-5 ID:SGTC_2716|Compound:st080858|FD-Score:-3.42|P-value:6.64E-4 ID:SGTC_2797|Compound:7859098|FD-Score:-3.29|P-value:9.85E-4 ID:SGTC_2884|Compound:9058502|FD-Score:3.52|P-value:5.16E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.84|P-value:1.66E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.60|P-value:3.66E-4 ID:SGTC_3021|Compound:9087658|FD-Score:3.63|P-value:3.49E-4 ID:SGTC_3095|Compound:9117207|FD-Score:4.44|P-value:1.70E-5 ID:SGTC_3107|Compound:9121808|FD-Score:-4.00|P-value:8.62E-5 ID:SGTC_3118|Compound:9124250|FD-Score:3.62|P-value:3.67E-4 ID:SGTC_3129|Compound:9127257|FD-Score:4.71|P-value:5.39E-6 ID:SGTC_3174|Compound:9107204|FD-Score:3.60|P-value:3.95E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-4.19|P-value:4.15E-5 ID:SGTC_3235|Compound:9132602|FD-Score:4.13|P-value:5.78E-5 ID:SGTC_3246|Compound:9135460|FD-Score:-4.10|P-value:5.85E-5 ID:SGTC_33|Compound:0986-0033|FD-Score:-3.34|P-value:8.56E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:4.61|P-value:8.49E-6 ID:SGTC_65|Compound:0798-0905|FD-Score:-4.94|P-value:1.77E-6 ID:SGTC_100|Compound:0086-0128|FD-Score:4.17|P-value:4.92E-5 ID:SGTC_180|Compound:1486-1293|FD-Score:6.17|P-value:4.02E-9 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.43|P-value:6.37E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:-5.62|P-value:6.68E-8 ID:SGTC_595|Compound:3909-8003|FD-Score:3.68|P-value:3.01E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:5.41|P-value:2.17E-7 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.50|P-value:5.52E-4 ID:SGTC_657|Compound:3534-0529|FD-Score:-3.44|P-value:6.08E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:4.29|P-value:3.11E-5 ID:SGTC_815|Compound:1582-0027|FD-Score:-4.21|P-value:3.84E-5 ID:SGTC_881|Compound:0833-0569|FD-Score:3.63|P-value:3.51E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-4.74|P-value:4.37E-6 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.62|P-value:8.00E-6 ID:SGTC_1046|Compound:2001-0062|FD-Score:3.71|P-value:2.68E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.82|P-value:1.82E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.38|P-value:8.13E-4 ID:SGTC_1319|Compound:1306-0155|FD-Score:-3.37|P-value:7.79E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:-3.64|P-value:3.15E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:5.90|P-value:1.74E-8 ID:SGTC_1634|Compound:st006576|FD-Score:-4.37|P-value:2.02E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.75|P-value:4.19E-6 ID:SGTC_1719|Compound:st032227|FD-Score:-4.31|P-value:2.57E-5 ID:SGTC_1900|Compound:st060200|FD-Score:3.36|P-value:8.53E-4 ID:SGTC_1959|Compound:st077091|FD-Score:5.16|P-value:7.23E-7 ID:SGTC_2001|Compound:4023502|FD-Score:4.54|P-value:1.13E-5 ID:SGTC_2005|Compound:4024620|FD-Score:3.48|P-value:5.78E-4 ID:SGTC_2020|Compound:4017018|FD-Score:-4.72|P-value:4.77E-6 ID:SGTC_2256|Compound:7954252|FD-Score:-4.86|P-value:2.53E-6 ID:SGTC_2302|Compound:7771753|FD-Score:5.11|P-value:8.93E-7 ID:SGTC_2348|Compound:9022543|FD-Score:3.44|P-value:6.62E-4 ID:SGTC_2351|Compound:9026858|FD-Score:5.14|P-value:7.67E-7 ID:SGTC_2395|Compound:5646831|FD-Score:3.66|P-value:3.15E-4 ID:SGTC_2462|Compound:5162487|FD-Score:3.84|P-value:1.66E-4 ID:SGTC_2470|Compound:5664817|FD-Score:3.37|P-value:8.38E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.93|P-value:1.21E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-3.71|P-value:2.44E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-5.60|P-value:7.30E-8 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:6.04|P-value:8.18E-9 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.52|P-value:1.23E-7 ID:SGTC_2696|Compound:st077691|FD-Score:-4.24|P-value:3.45E-5 ID:SGTC_2716|Compound:st080858|FD-Score:-3.42|P-value:6.64E-4 ID:SGTC_2797|Compound:7859098|FD-Score:-3.29|P-value:9.85E-4 ID:SGTC_2884|Compound:9058502|FD-Score:3.52|P-value:5.16E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.84|P-value:1.66E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.60|P-value:3.66E-4 ID:SGTC_3021|Compound:9087658|FD-Score:3.63|P-value:3.49E-4 ID:SGTC_3095|Compound:9117207|FD-Score:4.44|P-value:1.70E-5 ID:SGTC_3107|Compound:9121808|FD-Score:-4.00|P-value:8.62E-5 ID:SGTC_3118|Compound:9124250|FD-Score:3.62|P-value:3.67E-4 ID:SGTC_3129|Compound:9127257|FD-Score:4.71|P-value:5.39E-6 ID:SGTC_3174|Compound:9107204|FD-Score:3.60|P-value:3.95E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-4.19|P-value:4.15E-5 ID:SGTC_3235|Compound:9132602|FD-Score:4.13|P-value:5.78E-5 ID:SGTC_3246|Compound:9135460|FD-Score:-4.10|P-value:5.85E-5

Top fitness defect scores for YEL027W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_180 1486-1293 6.17 102.8 µM 4.02E-9 18 3.40E-10
2 SGTC_2520 obtusaquinone 6.04 4.7 µM 8.18E-9 1 7.63E-10
3 SGTC_1564 D-Arginine 5.90 115.0 µM 1.74E-8 9 1.81E-9
4 SGTC_2567 aureobasidin a 5.52 60.0 nM 1.23E-7 52 1.66E-8
5 SGTC_611 3453-1652 5.41 23.7 µM 2.17E-7 5 3.18E-8
6 SGTC_1959 st077091 5.16 55.8 µM 7.23E-7 RPP1 & pyrimidine depletion 17 1.25E-7
7 SGTC_2351 9026858 5.14 72.3 µM 7.67E-7 RNA processing & uracil transport 10 1.34E-7
8 SGTC_2302 7771753 5.11 200.0 µM 8.93E-7 19 1.59E-7
9 SGTC_3129 9127257 4.71 49.5 µM 5.39E-6 8 1.23E-6
10 SGTC_1002 4049-0206 4.62 172.0 µM 8.00E-6 13 1.93E-6
11 SGTC_43 0570-0105 4.61 72.5 µM 8.49E-6 ERG2 63 2.06E-6
12 SGTC_2001 4023502 4.54 57.7 µM 1.13E-5 mitochondrial processes 7 2.85E-6
13 SGTC_3095 9117207 4.44 49.5 µM 1.70E-5 4 4.54E-6
14 SGTC_801 0866-0028 4.29 10.1 µM 3.11E-5 18 9.04E-6
15 SGTC_100 0086-0128 4.17 49.6 µM 4.92E-5 copper-dependent oxidative stress 44 1.52E-5
16 SGTC_3235 9132602 4.13 49.5 µM 5.78E-5 20 1.82E-5
17 SGTC_2500 5-alpha-androstan-3,17-dione 3.93 61.4 µM 1.21E-4 40 4.23E-5
18 SGTC_2462 5162487 3.84 171.8 µM 1.66E-4 22 6.05E-5
19 SGTC_2950 9072895 3.84 21.5 µM 1.66E-4 43 6.07E-5
20 SGTC_1098 clofazimine 3.82 15.1 µM 1.82E-4 heme biosynthesis & mitochondrial translocase 27 6.73E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.137 1.63E-15 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.095 3.42E-8 YPR088C SRP54 het Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
0.094 4.91E-8 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.094 5.22E-8 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.088 3.04E-7 YGR078C PAC10 hom Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding
0.087 5.20E-7 YPR022C_p YPR022C_p hom Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
0.083 1.65E-6 YKL118W_d YKL118W_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2
0.082 1.81E-6 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.078 7.02E-6 YDL185W VMA1 hom Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
0.076 9.38E-6 YBR047W_p FMP23_p hom Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.075 1.36E-5 YJL208C NUC1 hom Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy
0.075 1.45E-5 YBR110W ALG1 het Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
0.074 2.02E-5 YLR370C ARC18 hom Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
0.073 2.57E-5 YOR230W WTM1 hom Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats
0.072 2.73E-5 YGL168W HUR1 hom Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene