YEL029C / BUD16

Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK)

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL029C / BUD16

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:3.98|P-value:6.18E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-4.37|P-value:2.11E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:-5.41|P-value:6.43E-6 ID:SGTC_115|Compound:0527-0192|FD-Score:4.97|P-value:2.78E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:-6.07|P-value:4.87E-7 ID:SGTC_118|Compound:0083-0097|FD-Score:6.24|P-value:2.08E-7 ID:SGTC_123|Compound:0083-0120|FD-Score:-5.75|P-value:1.75E-6 ID:SGTC_161|Compound:4065-0411|FD-Score:-4.81|P-value:5.17E-5 ID:SGTC_197|Compound:4544-0043|FD-Score:5.28|P-value:9.24E-6 ID:SGTC_363|Compound:0455-0021|FD-Score:4.64|P-value:8.43E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.35|P-value:7.21E-6 ID:SGTC_576|Compound:1073-0113|FD-Score:3.98|P-value:6.11E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:7.41|P-value:9.26E-10 ID:SGTC_673|Compound:1082-0474|FD-Score:5.69|P-value:1.96E-6 ID:SGTC_712|Compound:r015-0009|FD-Score:5.58|P-value:3.03E-6 ID:SGTC_723|Compound:4182-0001|FD-Score:-4.44|P-value:1.72E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:8.25|P-value:1.14E-11 ID:SGTC_831|Compound:k061-1017|FD-Score:6.83|P-value:1.53E-8 ID:SGTC_832|Compound:4083-0019|FD-Score:4.34|P-value:2.14E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:4.19|P-value:3.33E-4 ID:SGTC_880|Compound:0833-0487|FD-Score:-5.20|P-value:1.38E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.96|P-value:7.02E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:6.59|P-value:4.48E-8 ID:SGTC_928|Compound:2764-0428|FD-Score:6.10|P-value:3.70E-7 ID:SGTC_932|Compound:2857-0803|FD-Score:5.30|P-value:8.51E-6 ID:SGTC_1001|Compound:4048-4355|FD-Score:4.01|P-value:5.72E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.87|P-value:8.38E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.91|P-value:7.49E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.21|P-value:3.15E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:-3.98|P-value:6.60E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.94|P-value:7.41E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:8.19|P-value:1.61E-11 ID:SGTC_1814|Compound:st052110|FD-Score:-4.82|P-value:5.14E-5 ID:SGTC_1891|Compound:st059073|FD-Score:7.24|P-value:2.19E-9 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:8.22|P-value:1.33E-11 ID:SGTC_1906|Compound:st060206|FD-Score:-5.85|P-value:1.16E-6 ID:SGTC_1907|Compound:st060210|FD-Score:-6.02|P-value:5.89E-7 ID:SGTC_1934|Compound:st074720|FD-Score:-4.20|P-value:3.58E-4 ID:SGTC_1945|Compound:st075991|FD-Score:3.88|P-value:8.24E-4 ID:SGTC_1983|Compound:st071813|FD-Score:-6.82|P-value:1.88E-8 ID:SGTC_2005|Compound:4024620|FD-Score:4.97|P-value:2.74E-5 ID:SGTC_2027|Compound:5150920|FD-Score:3.97|P-value:6.27E-4 ID:SGTC_2077|Compound:5212015|FD-Score:4.07|P-value:4.78E-4 ID:SGTC_2085|Compound:5307070|FD-Score:4.79|P-value:5.01E-5 ID:SGTC_2109|Compound:5483026|FD-Score:4.72|P-value:6.33E-5 ID:SGTC_2261|Compound:9010538|FD-Score:-5.09|P-value:2.02E-5 ID:SGTC_2322|Compound:7413319|FD-Score:4.47|P-value:1.45E-4 ID:SGTC_2347|Compound:9049783|FD-Score:4.37|P-value:1.94E-4 ID:SGTC_2358|Compound:9037157|FD-Score:-5.92|P-value:8.92E-7 ID:SGTC_2365|Compound:9042475|FD-Score:6.64|P-value:3.60E-8 ID:SGTC_2495|Compound:cafestol|FD-Score:4.34|P-value:2.11E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:6.02|P-value:5.19E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.50|P-value:1.45E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:-3.88|P-value:8.77E-4 ID:SGTC_2699|Compound:st077704|FD-Score:4.71|P-value:6.63E-5 ID:SGTC_2834|Compound:9001755|FD-Score:8.80|P-value:5.12E-13 ID:SGTC_2835|Compound:9006588|FD-Score:3.87|P-value:8.29E-4 ID:SGTC_2947|Compound:9072639|FD-Score:5.46|P-value:4.71E-6 ID:SGTC_23|Compound:0213-0011|FD-Score:3.98|P-value:6.18E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-4.37|P-value:2.11E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:-5.41|P-value:6.43E-6 ID:SGTC_115|Compound:0527-0192|FD-Score:4.97|P-value:2.78E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:-6.07|P-value:4.87E-7 ID:SGTC_118|Compound:0083-0097|FD-Score:6.24|P-value:2.08E-7 ID:SGTC_123|Compound:0083-0120|FD-Score:-5.75|P-value:1.75E-6 ID:SGTC_161|Compound:4065-0411|FD-Score:-4.81|P-value:5.17E-5 ID:SGTC_197|Compound:4544-0043|FD-Score:5.28|P-value:9.24E-6 ID:SGTC_363|Compound:0455-0021|FD-Score:4.64|P-value:8.43E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.35|P-value:7.21E-6 ID:SGTC_576|Compound:1073-0113|FD-Score:3.98|P-value:6.11E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:7.41|P-value:9.26E-10 ID:SGTC_673|Compound:1082-0474|FD-Score:5.69|P-value:1.96E-6 ID:SGTC_712|Compound:r015-0009|FD-Score:5.58|P-value:3.03E-6 ID:SGTC_723|Compound:4182-0001|FD-Score:-4.44|P-value:1.72E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:8.25|P-value:1.14E-11 ID:SGTC_831|Compound:k061-1017|FD-Score:6.83|P-value:1.53E-8 ID:SGTC_832|Compound:4083-0019|FD-Score:4.34|P-value:2.14E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:4.19|P-value:3.33E-4 ID:SGTC_880|Compound:0833-0487|FD-Score:-5.20|P-value:1.38E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.96|P-value:7.02E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:6.59|P-value:4.48E-8 ID:SGTC_928|Compound:2764-0428|FD-Score:6.10|P-value:3.70E-7 ID:SGTC_932|Compound:2857-0803|FD-Score:5.30|P-value:8.51E-6 ID:SGTC_1001|Compound:4048-4355|FD-Score:4.01|P-value:5.72E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.87|P-value:8.38E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:3.91|P-value:7.49E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.21|P-value:3.15E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:-3.98|P-value:6.60E-4 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.94|P-value:7.41E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:8.19|P-value:1.61E-11 ID:SGTC_1814|Compound:st052110|FD-Score:-4.82|P-value:5.14E-5 ID:SGTC_1891|Compound:st059073|FD-Score:7.24|P-value:2.19E-9 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:8.22|P-value:1.33E-11 ID:SGTC_1906|Compound:st060206|FD-Score:-5.85|P-value:1.16E-6 ID:SGTC_1907|Compound:st060210|FD-Score:-6.02|P-value:5.89E-7 ID:SGTC_1934|Compound:st074720|FD-Score:-4.20|P-value:3.58E-4 ID:SGTC_1945|Compound:st075991|FD-Score:3.88|P-value:8.24E-4 ID:SGTC_1983|Compound:st071813|FD-Score:-6.82|P-value:1.88E-8 ID:SGTC_2005|Compound:4024620|FD-Score:4.97|P-value:2.74E-5 ID:SGTC_2027|Compound:5150920|FD-Score:3.97|P-value:6.27E-4 ID:SGTC_2077|Compound:5212015|FD-Score:4.07|P-value:4.78E-4 ID:SGTC_2085|Compound:5307070|FD-Score:4.79|P-value:5.01E-5 ID:SGTC_2109|Compound:5483026|FD-Score:4.72|P-value:6.33E-5 ID:SGTC_2261|Compound:9010538|FD-Score:-5.09|P-value:2.02E-5 ID:SGTC_2322|Compound:7413319|FD-Score:4.47|P-value:1.45E-4 ID:SGTC_2347|Compound:9049783|FD-Score:4.37|P-value:1.94E-4 ID:SGTC_2358|Compound:9037157|FD-Score:-5.92|P-value:8.92E-7 ID:SGTC_2365|Compound:9042475|FD-Score:6.64|P-value:3.60E-8 ID:SGTC_2495|Compound:cafestol|FD-Score:4.34|P-value:2.11E-4 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:6.02|P-value:5.19E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.50|P-value:1.45E-4 ID:SGTC_2650|Compound:prasterone|FD-Score:-3.88|P-value:8.77E-4 ID:SGTC_2699|Compound:st077704|FD-Score:4.71|P-value:6.63E-5 ID:SGTC_2834|Compound:9001755|FD-Score:8.80|P-value:5.12E-13 ID:SGTC_2835|Compound:9006588|FD-Score:3.87|P-value:8.29E-4 ID:SGTC_2947|Compound:9072639|FD-Score:5.46|P-value:4.71E-6

Top fitness defect scores for YEL029C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2834 9001755 8.80 58.4 µM 5.12E-13 4 7.12E-19
2 SGTC_830 4076-0286 8.25 57.8 µM 1.14E-11 translation 2 7.85E-17
3 SGTC_1894 3',6'-dihydroxyflavone 8.22 78.7 µM 1.33E-11 1 9.90E-17
4 SGTC_1450 4112-4260 8.19 37.6 µM 1.61E-11 calcium & mitochondrial duress 7 1.32E-16
5 SGTC_625 k213-0058 7.41 121.0 µM 9.26E-10 translation 19 6.09E-14
6 SGTC_1891 st059073 7.24 26.2 µM 2.19E-9 TSC3-RPN4 2 2.24E-13
7 SGTC_831 k061-1017 6.83 217.0 µM 1.53E-8 translation 6 4.23E-12
8 SGTC_2365 9042475 6.64 200.0 µM 3.60E-8 5 1.53E-11
9 SGTC_908 3448-7491 6.59 47.7 µM 4.48E-8 mitochondrial stress 35 2.13E-11
10 SGTC_118 0083-0097 6.24 135.3 µM 2.08E-7 translation 17 2.16E-10
11 SGTC_928 2764-0428 6.10 58.7 µM 3.70E-7 cell wall 14 5.15E-10
12 SGTC_2506 ethyl hematommate 6.02 54.2 µM 5.19E-7 RPP1 & pyrimidine depletion 3 8.55E-10
13 SGTC_673 1082-0474 5.69 110.0 µM 1.96E-6 RSC complex & mRNA processing 26 6.32E-9
14 SGTC_712 r015-0009 5.58 80.6 µM 3.03E-6 13 1.22E-8
15 SGTC_2947 9072639 5.46 7.7 µM 4.71E-6 tubulin folding & SWR complex 13 2.36E-8
16 SGTC_445 e6 berbamine 5.35 66.0 µM 7.21E-6 NEO1-PIK1 46 4.47E-8
17 SGTC_932 2857-0803 5.30 154.0 µM 8.51E-6 11 5.74E-8
18 SGTC_197 4544-0043 5.28 41.6 µM 9.24E-6 endomembrane recycling 23 6.49E-8
19 SGTC_2005 4024620 4.97 188.0 µM 2.74E-5 7 3.31E-7
20 SGTC_115 0527-0192 4.97 112.0 µM 2.78E-5 33 3.39E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.150 2.28E-18 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.137 1.38E-15 YDR516C EMI2 hom Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.132 1.56E-14 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.124 6.09E-13 YGL186C TPN1 hom Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
0.117 8.60E-12 YBR191W RPL21A hom Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
0.111 1.14E-10 YPL062W_d YPL062W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation
0.111 1.35E-10 YPL061W ALD6 hom Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
0.110 1.77E-10 YGR285C ZUO1 hom Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.109 2.79E-10 YBL032W HEK2 hom RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K
0.108 3.10E-10 YPR132W RPS23B hom Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
0.108 4.15E-10 YOR078W BUD21 hom Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
0.107 5.97E-10 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.106 8.31E-10 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.103 2.42E-9 YIR026C YVH1 hom Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases
0.101 4.93E-9 YJL189W RPL39 hom Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog