YEL032W / MCM3

Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex

Zygosity: Heterozygous strain
fixedexpanded
Profile for YEL032W / MCM3

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:4.23|P-value:5.83E-5 ID:SGTC_126|Compound:0417-1624|FD-Score:3.72|P-value:3.56E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:5.84|P-value:5.47E-8 ID:SGTC_145|Compound:3737-0113|FD-Score:6.68|P-value:5.93E-10 ID:SGTC_264|Compound:promethazine|FD-Score:9.31|P-value:1.22E-17 ID:SGTC_999|Compound:4022-0066|FD-Score:4.34|P-value:3.99E-5 ID:SGTC_1210|Compound:3013-0140|FD-Score:-3.40|P-value:9.72E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:-4.01|P-value:1.28E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-3.67|P-value:4.17E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.47|P-value:7.96E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:-4.20|P-value:6.51E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.68|P-value:1.01E-5 ID:SGTC_1391|Compound:3442-0225|FD-Score:4.94|P-value:3.42E-6 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.10|P-value:9.72E-5 ID:SGTC_1435|Compound:4092-0789|FD-Score:-3.74|P-value:3.25E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:6.46|P-value:2.11E-9 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.70|P-value:3.82E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:4.54|P-value:1.81E-5 ID:SGTC_1515|Compound:3460-0031|FD-Score:5.96|P-value:2.94E-8 ID:SGTC_1519|Compound:esterone|FD-Score:4.32|P-value:4.17E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.94|P-value:1.71E-4 ID:SGTC_1626|Compound:st003712|FD-Score:3.89|P-value:2.00E-4 ID:SGTC_1763|Compound:st044482|FD-Score:4.15|P-value:8.04E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:7.51|P-value:4.17E-12 ID:SGTC_2017|Compound:5132927|FD-Score:3.65|P-value:4.43E-4 ID:SGTC_2022|Compound:5136654|FD-Score:-5.57|P-value:1.90E-7 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-5.54|P-value:2.21E-7 ID:SGTC_2424|Compound:5537928|FD-Score:5.97|P-value:2.83E-8 ID:SGTC_2467|Compound:5474540|FD-Score:4.71|P-value:8.98E-6 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-4.01|P-value:1.31E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:-3.48|P-value:7.56E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:5.38|P-value:4.87E-7 ID:SGTC_3026|Compound:9090633|FD-Score:-4.02|P-value:1.23E-4 ID:SGTC_3038|Compound:9091290|FD-Score:-4.18|P-value:6.96E-5 ID:SGTC_3040|Compound:9091894|FD-Score:4.27|P-value:5.13E-5 ID:SGTC_3094|Compound:9117742|FD-Score:3.76|P-value:3.07E-4 ID:SGTC_3147|Compound:9097027|FD-Score:4.05|P-value:1.15E-4 ID:SGTC_3158|Compound:9100007|FD-Score:3.59|P-value:5.50E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-4.25|P-value:5.40E-5 ID:SGTC_3211|Compound:9128781|FD-Score:4.52|P-value:1.94E-5 ID:SGTC_9|Compound:0092-0002|FD-Score:4.23|P-value:5.83E-5 ID:SGTC_126|Compound:0417-1624|FD-Score:3.72|P-value:3.56E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:5.84|P-value:5.47E-8 ID:SGTC_145|Compound:3737-0113|FD-Score:6.68|P-value:5.93E-10 ID:SGTC_264|Compound:promethazine|FD-Score:9.31|P-value:1.22E-17 ID:SGTC_999|Compound:4022-0066|FD-Score:4.34|P-value:3.99E-5 ID:SGTC_1210|Compound:3013-0140|FD-Score:-3.40|P-value:9.72E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:-4.01|P-value:1.28E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-3.67|P-value:4.17E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:3.47|P-value:7.96E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:-4.20|P-value:6.51E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.68|P-value:1.01E-5 ID:SGTC_1391|Compound:3442-0225|FD-Score:4.94|P-value:3.42E-6 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.10|P-value:9.72E-5 ID:SGTC_1435|Compound:4092-0789|FD-Score:-3.74|P-value:3.25E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:6.46|P-value:2.11E-9 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.70|P-value:3.82E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:4.54|P-value:1.81E-5 ID:SGTC_1515|Compound:3460-0031|FD-Score:5.96|P-value:2.94E-8 ID:SGTC_1519|Compound:esterone|FD-Score:4.32|P-value:4.17E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.94|P-value:1.71E-4 ID:SGTC_1626|Compound:st003712|FD-Score:3.89|P-value:2.00E-4 ID:SGTC_1763|Compound:st044482|FD-Score:4.15|P-value:8.04E-5 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:7.51|P-value:4.17E-12 ID:SGTC_2017|Compound:5132927|FD-Score:3.65|P-value:4.43E-4 ID:SGTC_2022|Compound:5136654|FD-Score:-5.57|P-value:1.90E-7 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-5.54|P-value:2.21E-7 ID:SGTC_2424|Compound:5537928|FD-Score:5.97|P-value:2.83E-8 ID:SGTC_2467|Compound:5474540|FD-Score:4.71|P-value:8.98E-6 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-4.01|P-value:1.31E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:-3.48|P-value:7.56E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:5.38|P-value:4.87E-7 ID:SGTC_3026|Compound:9090633|FD-Score:-4.02|P-value:1.23E-4 ID:SGTC_3038|Compound:9091290|FD-Score:-4.18|P-value:6.96E-5 ID:SGTC_3040|Compound:9091894|FD-Score:4.27|P-value:5.13E-5 ID:SGTC_3094|Compound:9117742|FD-Score:3.76|P-value:3.07E-4 ID:SGTC_3147|Compound:9097027|FD-Score:4.05|P-value:1.15E-4 ID:SGTC_3158|Compound:9100007|FD-Score:3.59|P-value:5.50E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-4.25|P-value:5.40E-5 ID:SGTC_3211|Compound:9128781|FD-Score:4.52|P-value:1.94E-5

Top fitness defect scores for YEL032W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_264 promethazine 9.31 12.5 mM 1.22E-17 DNA intercalators 6 6.66E-21 0.06
2 SGTC_1926 methyl fluorone black 7.51 53.6 µM 4.17E-12 DNA intercalators 5 3.03E-14 0.19
3 SGTC_145 3737-0113 6.68 38.4 µM 5.93E-10 DNA intercalators 11 1.17E-11 0.08
4 SGTC_1468 k284-1086 6.46 134.0 µM 2.11E-9 DNA intercalators 3 5.40E-11 0.34
5 SGTC_2424 5537928 5.97 59.2 µM 2.83E-8 1 1.22E-9 0.68
6 SGTC_1515 3460-0031 5.96 42.1 µM 2.94E-8 DNA intercalators 3 1.27E-9 1.57
7 SGTC_144 4161-2749 5.84 112.4 µM 5.47E-8 DNA intercalators 4 2.69E-9 0.46
8 SGTC_2592 piperlongumine 5.38 83.3 µM 4.87E-7 TSC3-RPN4 2 3.71E-8 0.86
9 SGTC_1391 3442-0225 4.94 151.0 µM 3.42E-6 2 3.84E-7 0.98
10 SGTC_2467 5474540 4.71 200.0 µM 8.98E-6 2 1.22E-6 0.23
11 SGTC_1290 0986-0249 4.68 10.2 µM 1.01E-5 DNA intercalators 5 1.41E-6 0.04
12 SGTC_1494 4358-1428 4.54 177.0 µM 1.81E-5 6 2.83E-6 0.92
13 SGTC_3211 9128781 4.52 49.5 µM 1.94E-5 11 3.06E-6 0.24
14 SGTC_999 4022-0066 4.34 22.7 µM 3.99E-5 Golgi 9 7.26E-6 0.15
15 SGTC_1519 esterone 4.32 74.0 µM 4.17E-5 DNA intercalators 5 7.65E-6 0.39
16 SGTC_3040 9091894 4.27 49.5 µM 5.13E-5 DNA intercalators 9 9.82E-6 0.38
17 SGTC_9 0092-0002 4.23 10.4 µM 5.83E-5 12 1.14E-5 0.23
18 SGTC_1763 st044482 4.15 94.5 µM 8.04E-5 copper-dependent oxidative stress 3 1.68E-5 0.42
19 SGTC_1417 3966-0327 4.10 90.4 µM 9.72E-5 2 2.11E-5 0.63
20 SGTC_3147 9097027 4.05 49.5 µM 1.15E-4 5 2.57E-5 0.14

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.434 5.15E-154 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.410 1.26E-135 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.403 6.77E-131 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.378 5.03E-114 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.363 5.83E-105 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.347 2.84E-95 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.336 1.96E-89 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.336 7.21E-89 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.310 1.49E-75 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.282 2.86E-62 YMR262W_p YMR262W_p hom Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.254 2.20E-50 YLR133W CKI1 hom Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
0.249 1.92E-48 YGL041C_d YGL041C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.244 1.10E-46 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.233 1.08E-42 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.232 2.45E-42 YDL102W POL3 het Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)