HIPHOP chemogenomics database

YEL036C / ANP1

Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol

Zygosity: Homozygous strain

fixedexpanded
Profile for YEL036C / ANP1

Click on Significant Values for Screen Details

Top fitness defect scores for YEL036C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2583	asiatic acid	9.92	100.0 µM	1.49E-18		1	1.75E-23
2	SGTC_351	caspofungin	8.73	25.0 nM	9.24E-15	cell wall	12	1.31E-18
3	SGTC_445	e6 berbamine	8.05	66.0 µM	8.19E-13	NEO1-PIK1	12	4.18E-16
4	SGTC_1236	0330-0013	7.68	258.0 µM	7.96E-12		1	7.77E-15
5	SGTC_2009	4012297	6.95	12.1 µM	5.71E-10	cell wall signaling	19	1.88E-12
6	SGTC_121	0557-0495	6.13	61.3 µM	3.96E-8	calcium & mitochondrial duress	18	4.35E-10
7	SGTC_1264	0783-0040	5.95	158.0 µM	9.53E-8		4	1.34E-9
8	SGTC_2399	5884133	5.86	200.0 µM	1.46E-7	cell wall signaling	29	2.32E-9
9	SGTC_1844	rapamycin	5.81	4.0 nM	1.81E-7		93	3.08E-9
10	SGTC_2007	4011539	5.74	71.8 µM	2.55E-7	cell wall signaling	27	4.76E-9
11	SGTC_878	0828-0289	5.73	13.7 µM	2.67E-7		17	5.04E-9
12	SGTC_2366	9070441	5.45	60.6 µM	9.16E-7		6	2.45E-8
13	SGTC_2242	6630449	5.29	61.3 µM	1.83E-6	Golgi	22	5.99E-8
14	SGTC_1495	4469-0029	4.99	237.0 µM	6.42E-6		70	2.98E-7
15	SGTC_2994	9053520	4.97	71.4 µM	7.13E-6	RPP1 & pyrimidine depletion	10	3.42E-7
16	SGTC_2582	agaric acid	4.72	100.0 µM	1.91E-5	redox potentiating	240	1.21E-6
17	SGTC_1597	7-benzyloxygramine	4.70	47.5 µM	2.03E-5	calcium & mitochondrial duress	76	1.30E-6
18	SGTC_908	3448-7491	4.61	47.7 µM	2.85E-5	mitochondrial stress	90	2.02E-6
19	SGTC_2415	st077333	4.48	57.7 µM	4.60E-5		30	3.72E-6
20	SGTC_663	1486-0733	4.48	13.9 µM	4.62E-5	cell wall signaling	39	3.75E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.205	4.47E-33	YJL183W	MNN11	hom	Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
0.201	7.58E-32	YGL027C	CWH41	hom	Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress
0.192	3.18E-29	YJR075W	HOC1	hom	Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
0.179	1.58E-25	YBR229C	ROT2	hom	Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.173	5.12E-24	YAL058W	CNE1	hom	Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.172	8.94E-24	YNL322C	KRE1	hom	Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.169	8.48E-23	YNL084C	END3	hom	EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
0.167	1.83E-22	YDR245W	MNN10	hom	Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family
0.163	1.97E-21	YMR200W	ROT1	het	Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.163	2.55E-21	YGR196C	FYV8	hom	Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.158	3.28E-20	YAL053W	FLC2	hom	Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.157	7.69E-20	YAL056C-A_d	YAL056C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.149	3.89E-18	YMR238W	DFG5	hom	Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
0.148	6.19E-18	YML115C	VAN1	hom	Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
0.139	6.82E-16	YOR002W	ALG6	hom	Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease