YEL051W / VMA8

Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL051W / VMA8

Click on Significant Values for Screen Details ID:SGTC_102|Compound:gallobenzophenone|FD-Score:4.42|P-value:1.82E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.78|P-value:2.14E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.36|P-value:8.81E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.25|P-value:2.57E-5 ID:SGTC_344|Compound:k064-0035|FD-Score:-3.87|P-value:1.10E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.53|P-value:5.04E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-4.53|P-value:7.92E-6 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:4.25|P-value:3.66E-5 ID:SGTC_464|Compound:bw-b 70c|FD-Score:4.17|P-value:4.97E-5 ID:SGTC_667|Compound:1498-1044|FD-Score:-3.30|P-value:7.88E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.73|P-value:2.54E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.56|P-value:4.58E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:4.07|P-value:7.22E-5 ID:SGTC_1608|Compound:st001473|FD-Score:3.95|P-value:1.13E-4 ID:SGTC_1629|Compound:st005085|FD-Score:3.97|P-value:1.07E-4 ID:SGTC_1636|Compound:st007707|FD-Score:3.58|P-value:4.23E-4 ID:SGTC_1656|Compound:st012921|FD-Score:4.17|P-value:5.00E-5 ID:SGTC_1660|Compound:st012960|FD-Score:4.23|P-value:3.91E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.78|P-value:2.09E-4 ID:SGTC_1911|Compound:st060208|FD-Score:3.78|P-value:2.13E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.80|P-value:1.99E-4 ID:SGTC_1981|Compound:st071826|FD-Score:3.66|P-value:3.26E-4 ID:SGTC_1985|Compound:st071382|FD-Score:3.56|P-value:4.62E-4 ID:SGTC_1991|Compound:st073000|FD-Score:4.28|P-value:3.23E-5 ID:SGTC_2117|Compound:5521686|FD-Score:3.49|P-value:5.69E-4 ID:SGTC_2124|Compound:5246320|FD-Score:-4.84|P-value:1.99E-6 ID:SGTC_2126|Compound:5251174|FD-Score:-4.22|P-value:2.86E-5 ID:SGTC_2163|Compound:5671233|FD-Score:3.54|P-value:4.82E-4 ID:SGTC_2302|Compound:7771753|FD-Score:3.58|P-value:4.26E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.39|P-value:7.85E-4 ID:SGTC_2466|Compound:5983409|FD-Score:3.96|P-value:1.10E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:4.23|P-value:3.94E-5 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-3.74|P-value:1.76E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.59|P-value:4.11E-4 ID:SGTC_2795|Compound:7780010|FD-Score:3.69|P-value:2.94E-4 ID:SGTC_2816|Compound:7982706|FD-Score:-3.31|P-value:7.82E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-3.23|P-value:0.00100 ID:SGTC_2953|Compound:9074162|FD-Score:-4.03|P-value:6.09E-5 ID:SGTC_3162|Compound:9099653|FD-Score:-3.55|P-value:3.43E-4 ID:SGTC_3330|Compound:9141424|FD-Score:3.63|P-value:3.63E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:4.42|P-value:1.82E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.78|P-value:2.14E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.36|P-value:8.81E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.25|P-value:2.57E-5 ID:SGTC_344|Compound:k064-0035|FD-Score:-3.87|P-value:1.10E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.53|P-value:5.04E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-4.53|P-value:7.92E-6 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:4.25|P-value:3.66E-5 ID:SGTC_464|Compound:bw-b 70c|FD-Score:4.17|P-value:4.97E-5 ID:SGTC_667|Compound:1498-1044|FD-Score:-3.30|P-value:7.88E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.73|P-value:2.54E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.56|P-value:4.58E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:4.07|P-value:7.22E-5 ID:SGTC_1608|Compound:st001473|FD-Score:3.95|P-value:1.13E-4 ID:SGTC_1629|Compound:st005085|FD-Score:3.97|P-value:1.07E-4 ID:SGTC_1636|Compound:st007707|FD-Score:3.58|P-value:4.23E-4 ID:SGTC_1656|Compound:st012921|FD-Score:4.17|P-value:5.00E-5 ID:SGTC_1660|Compound:st012960|FD-Score:4.23|P-value:3.91E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.78|P-value:2.09E-4 ID:SGTC_1911|Compound:st060208|FD-Score:3.78|P-value:2.13E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.80|P-value:1.99E-4 ID:SGTC_1981|Compound:st071826|FD-Score:3.66|P-value:3.26E-4 ID:SGTC_1985|Compound:st071382|FD-Score:3.56|P-value:4.62E-4 ID:SGTC_1991|Compound:st073000|FD-Score:4.28|P-value:3.23E-5 ID:SGTC_2117|Compound:5521686|FD-Score:3.49|P-value:5.69E-4 ID:SGTC_2124|Compound:5246320|FD-Score:-4.84|P-value:1.99E-6 ID:SGTC_2126|Compound:5251174|FD-Score:-4.22|P-value:2.86E-5 ID:SGTC_2163|Compound:5671233|FD-Score:3.54|P-value:4.82E-4 ID:SGTC_2302|Compound:7771753|FD-Score:3.58|P-value:4.26E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.39|P-value:7.85E-4 ID:SGTC_2466|Compound:5983409|FD-Score:3.96|P-value:1.10E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:4.23|P-value:3.94E-5 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-3.74|P-value:1.76E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.59|P-value:4.11E-4 ID:SGTC_2795|Compound:7780010|FD-Score:3.69|P-value:2.94E-4 ID:SGTC_2816|Compound:7982706|FD-Score:-3.31|P-value:7.82E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-3.23|P-value:0.00100 ID:SGTC_2953|Compound:9074162|FD-Score:-4.03|P-value:6.09E-5 ID:SGTC_3162|Compound:9099653|FD-Score:-3.55|P-value:3.43E-4 ID:SGTC_3330|Compound:9141424|FD-Score:3.63|P-value:3.63E-4

Top fitness defect scores for YEL051W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_102 gallobenzophenone 4.42 83.6 µM 1.82E-5 redox potentiating 36 4.94E-6
2 SGTC_1991 st073000 4.28 8.9 µM 3.23E-5 NEO1 67 9.42E-6
3 SGTC_454 lyso-paf C-16 4.25 6.7 µM 3.66E-5 13 1.08E-5
4 SGTC_1660 st012960 4.23 28.1 µM 3.91E-5 iron homeostasis 30 1.17E-5
5 SGTC_2530 3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene 4.23 92.6 µM 3.94E-5 21 1.18E-5
6 SGTC_464 bw-b 70c 4.17 158.0 µM 4.97E-5 15 1.53E-5
7 SGTC_1656 st012921 4.17 11.0 µM 5.00E-5 25 1.54E-5
8 SGTC_1385 3091-4834 4.07 14.1 µM 7.22E-5 RPP1 & pyrimidine depletion 19 2.33E-5
9 SGTC_1629 st005085 3.97 44.9 µM 1.07E-4 RPP1 & pyrimidine depletion 36 3.65E-5
10 SGTC_2466 5983409 3.96 37.1 µM 1.10E-4 17 3.76E-5
11 SGTC_1608 st001473 3.95 40.5 µM 1.13E-4 34 3.86E-5
12 SGTC_1917 st066507 3.80 28.5 µM 1.99E-4 Golgi 43 7.32E-5
13 SGTC_1785 bathophenanthroline 3.78 24.0 µM 2.09E-4 heme requiring 57 7.72E-5
14 SGTC_1911 st060208 3.78 74.3 µM 2.13E-4 41 7.88E-5
15 SGTC_115 0527-0192 3.78 112.0 µM 2.14E-4 81 7.95E-5
16 SGTC_756 0508-1999 3.73 28.3 µM 2.54E-4 RPP1 & pyrimidine depletion 35 9.64E-5
17 SGTC_2795 7780010 3.69 54.5 µM 2.94E-4 37 1.14E-4
18 SGTC_1981 st071826 3.66 47.0 µM 3.26E-4 20 1.28E-4
19 SGTC_3330 9141424 3.63 71.4 µM 3.63E-4 amide catabolism 27 1.44E-4
20 SGTC_2615 purpurogallin-4-carboxylic acid 3.59 100.0 µM 4.11E-4 redox potentiating 77 1.66E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.138 1.13E-15 YBR151W APD1 hom Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus
0.121 1.86E-12 YPR036W VMA13 hom Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
0.115 2.66E-11 YHR026W VMA16 hom Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
0.101 5.29E-9 YMR253C_p YMR253C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
0.095 3.42E-8 YKL118W_d YKL118W_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2
0.089 2.67E-7 YML073C RPL6A hom Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
0.086 5.62E-7 YOL115W PAP2 hom Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p
0.086 6.45E-7 YKR004C ECM9 het Non-essential protein of unknown function
0.085 7.22E-7 YBR017C KAP104 hom Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis
0.083 1.38E-6 YGR280C PXR1 het Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
0.081 2.70E-6 YDL185W VMA1 hom Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
0.080 3.36E-6 YCL012C YCL012C hom Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene
0.080 3.66E-6 YLR148W PEP3 hom Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
0.079 4.81E-6 YGL154C LYS5 hom Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine
0.077 8.86E-6 YGL007C-A_p YGL007C-A_p hom Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding