YEL054C / RPL12A

Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL054C / RPL12A

Click on Significant Values for Screen Details ID:SGTC_118|Compound:0083-0097|FD-Score:4.26|P-value:8.11E-6 ID:SGTC_127|Compound:0180-0333|FD-Score:3.05|P-value:9.94E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.31|P-value:4.03E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-3.12|P-value:9.47E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.82|P-value:5.46E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.69|P-value:9.56E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:4.07|P-value:1.95E-5 ID:SGTC_822|Compound:1487-0950|FD-Score:4.38|P-value:4.70E-6 ID:SGTC_830|Compound:4076-0286|FD-Score:5.02|P-value:1.93E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.97|P-value:2.99E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:8.31|P-value:2.74E-17 ID:SGTC_845|Compound:0330-0138|FD-Score:-3.26|P-value:5.90E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.17|P-value:6.49E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:-4.10|P-value:2.13E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.05|P-value:9.84E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:-3.24|P-value:6.23E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.09|P-value:8.58E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.28|P-value:5.32E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.09|P-value:8.57E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:-3.76|P-value:8.93E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.54|P-value:1.68E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:-4.20|P-value:1.41E-5 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.37|P-value:3.23E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.15|P-value:1.71E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.26|P-value:5.90E-4 ID:SGTC_1547|Compound:harmine|FD-Score:-3.11|P-value:9.78E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.00|P-value:3.29E-5 ID:SGTC_1716|Compound:st033144|FD-Score:-3.73|P-value:9.98E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.48|P-value:3.79E-6 ID:SGTC_1900|Compound:st060200|FD-Score:-3.16|P-value:8.27E-4 ID:SGTC_1915|Compound:st067105|FD-Score:-3.41|P-value:3.33E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-3.17|P-value:8.03E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-3.33|P-value:4.46E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:-3.37|P-value:3.86E-4 ID:SGTC_2316|Compound:5844804|FD-Score:3.67|P-value:1.03E-4 ID:SGTC_2341|Compound:7987486|FD-Score:3.63|P-value:1.21E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-4.65|P-value:1.73E-6 ID:SGTC_2555|Compound:cholic acid|FD-Score:3.25|P-value:4.94E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:5.64|P-value:6.34E-9 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:6.13|P-value:3.11E-10 ID:SGTC_2568|Compound:diosmetin|FD-Score:-3.44|P-value:2.98E-4 ID:SGTC_2570|Compound:licareol|FD-Score:-6.56|P-value:2.53E-11 ID:SGTC_2680|Compound:butyl paraben|FD-Score:3.82|P-value:5.48E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.82|P-value:3.08E-19 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.58|P-value:1.44E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:-3.19|P-value:7.38E-4 ID:SGTC_2858|Compound:9025781|FD-Score:-3.13|P-value:9.17E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.24|P-value:1.16E-5 ID:SGTC_2905|Compound:7799065|FD-Score:-3.12|P-value:9.56E-4 ID:SGTC_2906|Compound:7926292|FD-Score:3.80|P-value:6.06E-5 ID:SGTC_2909|Compound:7941780|FD-Score:4.10|P-value:1.68E-5 ID:SGTC_3007|Compound:9078383|FD-Score:3.12|P-value:7.83E-4 ID:SGTC_3013|Compound:9080133|FD-Score:3.46|P-value:2.28E-4 ID:SGTC_3017|Compound:9080681|FD-Score:3.22|P-value:5.51E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.45|P-value:2.35E-4 ID:SGTC_3140|Compound:9094243|FD-Score:3.60|P-value:1.37E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-3.96|P-value:3.91E-5 ID:SGTC_3171|Compound:9103192|FD-Score:-3.18|P-value:7.70E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.16|P-value:8.32E-4 ID:SGTC_3203|Compound:9113543|FD-Score:-3.85|P-value:6.17E-5 ID:SGTC_3204|Compound:9114183|FD-Score:-3.47|P-value:2.72E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.26|P-value:8.11E-6 ID:SGTC_127|Compound:0180-0333|FD-Score:3.05|P-value:9.94E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.31|P-value:4.03E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-3.12|P-value:9.47E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.82|P-value:5.46E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.69|P-value:9.56E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:4.07|P-value:1.95E-5 ID:SGTC_822|Compound:1487-0950|FD-Score:4.38|P-value:4.70E-6 ID:SGTC_830|Compound:4076-0286|FD-Score:5.02|P-value:1.93E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.97|P-value:2.99E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:8.31|P-value:2.74E-17 ID:SGTC_845|Compound:0330-0138|FD-Score:-3.26|P-value:5.90E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.17|P-value:6.49E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:-4.10|P-value:2.13E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.05|P-value:9.84E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:-3.24|P-value:6.23E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:3.09|P-value:8.58E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.28|P-value:5.32E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.09|P-value:8.57E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:-3.76|P-value:8.93E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.54|P-value:1.68E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:-4.20|P-value:1.41E-5 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.37|P-value:3.23E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.15|P-value:1.71E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.26|P-value:5.90E-4 ID:SGTC_1547|Compound:harmine|FD-Score:-3.11|P-value:9.78E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-4.00|P-value:3.29E-5 ID:SGTC_1716|Compound:st033144|FD-Score:-3.73|P-value:9.98E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.48|P-value:3.79E-6 ID:SGTC_1900|Compound:st060200|FD-Score:-3.16|P-value:8.27E-4 ID:SGTC_1915|Compound:st067105|FD-Score:-3.41|P-value:3.33E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-3.17|P-value:8.03E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-3.33|P-value:4.46E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:-3.37|P-value:3.86E-4 ID:SGTC_2316|Compound:5844804|FD-Score:3.67|P-value:1.03E-4 ID:SGTC_2341|Compound:7987486|FD-Score:3.63|P-value:1.21E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-4.65|P-value:1.73E-6 ID:SGTC_2555|Compound:cholic acid|FD-Score:3.25|P-value:4.94E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:5.64|P-value:6.34E-9 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:6.13|P-value:3.11E-10 ID:SGTC_2568|Compound:diosmetin|FD-Score:-3.44|P-value:2.98E-4 ID:SGTC_2570|Compound:licareol|FD-Score:-6.56|P-value:2.53E-11 ID:SGTC_2680|Compound:butyl paraben|FD-Score:3.82|P-value:5.48E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.82|P-value:3.08E-19 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.58|P-value:1.44E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:-3.19|P-value:7.38E-4 ID:SGTC_2858|Compound:9025781|FD-Score:-3.13|P-value:9.17E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.24|P-value:1.16E-5 ID:SGTC_2905|Compound:7799065|FD-Score:-3.12|P-value:9.56E-4 ID:SGTC_2906|Compound:7926292|FD-Score:3.80|P-value:6.06E-5 ID:SGTC_2909|Compound:7941780|FD-Score:4.10|P-value:1.68E-5 ID:SGTC_3007|Compound:9078383|FD-Score:3.12|P-value:7.83E-4 ID:SGTC_3013|Compound:9080133|FD-Score:3.46|P-value:2.28E-4 ID:SGTC_3017|Compound:9080681|FD-Score:3.22|P-value:5.51E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.45|P-value:2.35E-4 ID:SGTC_3140|Compound:9094243|FD-Score:3.60|P-value:1.37E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-3.96|P-value:3.91E-5 ID:SGTC_3171|Compound:9103192|FD-Score:-3.18|P-value:7.70E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.16|P-value:8.32E-4 ID:SGTC_3203|Compound:9113543|FD-Score:-3.85|P-value:6.17E-5 ID:SGTC_3204|Compound:9114183|FD-Score:-3.47|P-value:2.72E-4

Top fitness defect scores for YEL054C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2688 kanamycin b 8.82 96.7 µM 3.08E-19 calcium & mitochondrial duress 47 5.68E-19
2 SGTC_832 4083-0019 8.31 164.0 µM 2.74E-17 translation 5 4.79E-17
3 SGTC_2565 isoliquiritigenin 6.13 100.0 µM 3.11E-10 9 4.42E-10
4 SGTC_2563 tomatine 5.64 8.5 µM 6.34E-9 12 8.66E-9
5 SGTC_830 4076-0286 5.02 57.8 µM 1.93E-7 translation 32 2.52E-7
6 SGTC_822 1487-0950 4.38 154.0 µM 4.70E-6 6 5.86E-6
7 SGTC_118 0083-0097 4.26 135.3 µM 8.11E-6 translation 105 1.00E-5
8 SGTC_2909 7941780 4.10 10.5 µM 1.68E-5 19 2.06E-5
9 SGTC_800 3448-7362 4.07 155.0 µM 1.95E-5 translation 153 2.37E-5
10 SGTC_831 k061-1017 3.97 217.0 µM 2.99E-5 translation 64 3.62E-5
11 SGTC_422 cantharidin 3.82 155.0 µM 5.46E-5 cell wall signaling 60 6.56E-5
12 SGTC_2680 butyl paraben 3.82 22.2 µM 5.48E-5 amide catabolism 8 6.58E-5
13 SGTC_2906 7926292 3.80 26.4 µM 6.06E-5 28 7.27E-5
14 SGTC_751 0833-0568 3.69 350.0 µM 9.56E-5 30 1.14E-4
15 SGTC_2316 5844804 3.67 197.4 µM 1.03E-4 42 1.22E-4
16 SGTC_2341 7987486 3.63 176.7 µM 1.21E-4 26 1.44E-4
17 SGTC_3140 9094243 3.60 49.5 µM 1.37E-4 37 1.62E-4
18 SGTC_2690 usnic acid 3.58 100.0 µM 1.44E-4 38 1.71E-4
19 SGTC_1424 4048-1941 3.54 275.0 µM 1.68E-4 34 1.98E-4
20 SGTC_3013 9080133 3.46 71.4 µM 2.28E-4 42 2.68E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.255 7.53E-51 YGL246C RAI1 hom Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z
0.246 1.57E-47 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.246 2.75E-47 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.219 8.57E-38 YPL249C-A RPL36B hom Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication
0.207 7.28E-34 YJL189W RPL39 hom Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.197 1.11E-30 YDR417C_d YDR417C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W
0.191 8.43E-29 YJR145C RPS4A hom Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
0.187 7.47E-28 YLR068W FYV7 hom Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin
0.187 7.42E-28 YDL082W RPL13A hom Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication
0.183 1.13E-26 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.181 4.04E-26 YML061C PIF1 hom DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells
0.180 6.84E-26 YBR287W_p YBR287W_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
0.177 5.95E-25 YOR309C_d YOR309C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
0.175 1.39E-24 YMR193W MRPL24 hom Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.175 1.46E-24 YIR026C YVH1 hom Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases