YER005W / YND1

Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity

Zygosity: Homozygous strain
fixedexpanded
Profile for YER005W / YND1

Click on Significant Values for Screen Details ID:SGTC_87|Compound:0100-0052|FD-Score:4.27|P-value:1.17E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:3.31|P-value:5.06E-4 ID:SGTC_210|Compound:3551-0445|FD-Score:4.78|P-value:1.13E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:-3.18|P-value:9.22E-4 ID:SGTC_332|Compound:4112-0506|FD-Score:4.43|P-value:5.60E-6 ID:SGTC_406|Compound:estrone|FD-Score:3.53|P-value:2.30E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:6.36|P-value:1.56E-10 ID:SGTC_658|Compound:3253-0951|FD-Score:-3.49|P-value:3.10E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.56|P-value:2.41E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:3.26|P-value:6.14E-4 ID:SGTC_702|Compound:1488-0190|FD-Score:3.56|P-value:2.07E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:-7.51|P-value:7.69E-14 ID:SGTC_880|Compound:0833-0487|FD-Score:-3.29|P-value:6.31E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.01|P-value:4.22E-5 ID:SGTC_1297|Compound:1068-0111|FD-Score:3.55|P-value:2.19E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.82|P-value:7.62E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:3.69|P-value:1.27E-4 ID:SGTC_1817|Compound:st052116|FD-Score:3.90|P-value:5.62E-5 ID:SGTC_1914|Compound:st062367|FD-Score:4.63|P-value:2.27E-6 ID:SGTC_2089|Compound:5281248|FD-Score:3.50|P-value:2.56E-4 ID:SGTC_2181|Compound:6316240|FD-Score:4.73|P-value:1.43E-6 ID:SGTC_2286|Compound:7945697|FD-Score:4.77|P-value:1.15E-6 ID:SGTC_2313|Compound:7726885|FD-Score:5.02|P-value:3.39E-7 ID:SGTC_2357|Compound:9067248|FD-Score:-3.37|P-value:4.69E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:-5.17|P-value:1.85E-7 ID:SGTC_2511|Compound:berberine|FD-Score:-3.80|P-value:9.66E-5 ID:SGTC_2526|Compound:patulin|FD-Score:6.59|P-value:3.54E-11 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.79|P-value:8.72E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-4.29|P-value:1.29E-5 ID:SGTC_2591|Compound:skatole|FD-Score:-4.17|P-value:2.16E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.61|P-value:1.75E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:5.43|P-value:3.89E-8 ID:SGTC_2792|Compound:7535235|FD-Score:-3.18|P-value:9.08E-4 ID:SGTC_2798|Compound:7813637|FD-Score:4.84|P-value:8.13E-7 ID:SGTC_2852|Compound:9023854|FD-Score:-3.20|P-value:8.55E-4 ID:SGTC_2857|Compound:9024691|FD-Score:-3.25|P-value:7.16E-4 ID:SGTC_2878|Compound:9043973|FD-Score:3.82|P-value:7.60E-5 ID:SGTC_2889|Compound:9059656|FD-Score:3.24|P-value:6.56E-4 ID:SGTC_3281|Compound:9140613|FD-Score:7.04|P-value:1.71E-12 ID:SGTC_87|Compound:0100-0052|FD-Score:4.27|P-value:1.17E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:3.31|P-value:5.06E-4 ID:SGTC_210|Compound:3551-0445|FD-Score:4.78|P-value:1.13E-6 ID:SGTC_256|Compound:clotrimazole|FD-Score:-3.18|P-value:9.22E-4 ID:SGTC_332|Compound:4112-0506|FD-Score:4.43|P-value:5.60E-6 ID:SGTC_406|Compound:estrone|FD-Score:3.53|P-value:2.30E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:6.36|P-value:1.56E-10 ID:SGTC_658|Compound:3253-0951|FD-Score:-3.49|P-value:3.10E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.56|P-value:2.41E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:3.26|P-value:6.14E-4 ID:SGTC_702|Compound:1488-0190|FD-Score:3.56|P-value:2.07E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:-7.51|P-value:7.69E-14 ID:SGTC_880|Compound:0833-0487|FD-Score:-3.29|P-value:6.31E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.01|P-value:4.22E-5 ID:SGTC_1297|Compound:1068-0111|FD-Score:3.55|P-value:2.19E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.82|P-value:7.62E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:3.69|P-value:1.27E-4 ID:SGTC_1817|Compound:st052116|FD-Score:3.90|P-value:5.62E-5 ID:SGTC_1914|Compound:st062367|FD-Score:4.63|P-value:2.27E-6 ID:SGTC_2089|Compound:5281248|FD-Score:3.50|P-value:2.56E-4 ID:SGTC_2181|Compound:6316240|FD-Score:4.73|P-value:1.43E-6 ID:SGTC_2286|Compound:7945697|FD-Score:4.77|P-value:1.15E-6 ID:SGTC_2313|Compound:7726885|FD-Score:5.02|P-value:3.39E-7 ID:SGTC_2357|Compound:9067248|FD-Score:-3.37|P-value:4.69E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:-5.17|P-value:1.85E-7 ID:SGTC_2511|Compound:berberine|FD-Score:-3.80|P-value:9.66E-5 ID:SGTC_2526|Compound:patulin|FD-Score:6.59|P-value:3.54E-11 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.79|P-value:8.72E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-4.29|P-value:1.29E-5 ID:SGTC_2591|Compound:skatole|FD-Score:-4.17|P-value:2.16E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.61|P-value:1.75E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:5.43|P-value:3.89E-8 ID:SGTC_2792|Compound:7535235|FD-Score:-3.18|P-value:9.08E-4 ID:SGTC_2798|Compound:7813637|FD-Score:4.84|P-value:8.13E-7 ID:SGTC_2852|Compound:9023854|FD-Score:-3.20|P-value:8.55E-4 ID:SGTC_2857|Compound:9024691|FD-Score:-3.25|P-value:7.16E-4 ID:SGTC_2878|Compound:9043973|FD-Score:3.82|P-value:7.60E-5 ID:SGTC_2889|Compound:9059656|FD-Score:3.24|P-value:6.56E-4 ID:SGTC_3281|Compound:9140613|FD-Score:7.04|P-value:1.71E-12

Top fitness defect scores for YER005W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3281 9140613 7.04 49.5 µM 1.71E-12 RPP1 & pyrimidine depletion 2 9.79E-13
2 SGTC_2526 patulin 6.59 37.6 µM 3.54E-11 21 2.18E-11
3 SGTC_524 r073-0010 6.36 27.8 µM 1.56E-10 13 9.96E-11
4 SGTC_2626 ethyl orsellinate 5.43 100.0 µM 3.89E-8 8 2.84E-8
5 SGTC_2313 7726885 5.02 200.0 µM 3.39E-7 RPP1 & pyrimidine depletion 16 2.61E-7
6 SGTC_2798 7813637 4.84 45.5 µM 8.13E-7 13 6.39E-7
7 SGTC_210 3551-0445 4.78 56.8 µM 1.13E-6 41 8.97E-7
8 SGTC_2286 7945697 4.77 125.0 µM 1.15E-6 32 9.08E-7
9 SGTC_2181 6316240 4.73 54.3 µM 1.43E-6 12 1.14E-6
10 SGTC_1914 st062367 4.63 72.6 µM 2.27E-6 10 1.83E-6
11 SGTC_332 4112-0506 4.43 40.9 µM 5.60E-6 18 4.60E-6
12 SGTC_87 0100-0052 4.27 35.5 µM 1.17E-5 88 9.74E-6
13 SGTC_1817 st052116 3.90 64.4 µM 5.62E-5 RNA processing & uracil transport 14 4.87E-5
14 SGTC_2878 9043973 3.82 26.0 µM 7.60E-5 21 6.62E-5
15 SGTC_1345 1488-0192 3.82 31.4 µM 7.62E-5 20 6.64E-5
16 SGTC_2578 7,8-dihydroxyflavone 3.79 64.4 µM 8.72E-5 33 7.63E-5
17 SGTC_1568 digitonin 3.69 820.0 nM 1.27E-4 sphingolipid biosynthesis & PDR1 41 1.12E-4
18 SGTC_2621 lithochol-11-enic acid 3.61 100.0 µM 1.75E-4 38 1.55E-4
19 SGTC_702 1488-0190 3.56 94.0 µM 2.07E-4 amide catabolism 21 1.84E-4
20 SGTC_1297 1068-0111 3.55 14.1 µM 2.19E-4 19 1.95E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.128 9.01E-14 YML007W YAP1 hom Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.122 1.39E-12 YBR014C GRX7 hom Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication
0.115 2.87E-11 YER011W TIR1 hom Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking
0.112 8.92E-11 YBR008C FLR1 hom Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress
0.111 1.27E-10 YAL024C LTE1 hom Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
0.107 5.48E-10 YBR216C YBP1 hom Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication
0.106 7.11E-10 YNL298W CLA4 hom Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
0.102 3.52E-9 YLR187W SKG3 hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
0.097 1.75E-8 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.095 3.33E-8 YDR192C NUP42 hom FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p
0.092 9.40E-8 YOR349W CIN1 hom Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.090 1.58E-7 YOR235W_d IRC13_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci
0.090 2.04E-7 YBR047W_p FMP23_p hom Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.088 3.60E-7 YMR102C_p YMR102C_p hom Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.085 7.19E-7 YOR244W ESA1 het Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy