YER007W / PAC2

Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl

Zygosity: Homozygous strain
fixedexpanded
Profile for YER007W / PAC2

Click on Significant Values for Screen Details ID:SGTC_12|Compound:0849-0099|FD-Score:7.81|P-value:2.27E-15 ID:SGTC_149|Compound:3970-0822|FD-Score:29.10|P-value:7.56E-188 ID:SGTC_163|Compound:1327-0036|FD-Score:7.29|P-value:1.33E-13 ID:SGTC_210|Compound:3551-0445|FD-Score:40.40|P-value:0 ID:SGTC_212|Compound:3807-4682|FD-Score:34.10|P-value:6.59E-257 ID:SGTC_229|Compound:benomyl|FD-Score:46.40|P-value:0 ID:SGTC_230|Compound:MPP+|FD-Score:5.70|P-value:5.38E-9 ID:SGTC_236|Compound:1509-0011|FD-Score:4.55|P-value:2.49E-6 ID:SGTC_286|Compound:k060-0067|FD-Score:22.80|P-value:3.27E-116 ID:SGTC_300|Compound:r073-0011|FD-Score:3.38|P-value:3.47E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:12.80|P-value:3.58E-38 ID:SGTC_376|Compound:3970-0862|FD-Score:6.66|P-value:1.15E-11 ID:SGTC_377|Compound:3970-0964|FD-Score:6.66|P-value:1.19E-11 ID:SGTC_379|Compound:3970-0972|FD-Score:10.90|P-value:2.99E-28 ID:SGTC_524|Compound:r073-0010|FD-Score:4.70|P-value:1.19E-6 ID:SGTC_589|Compound:1227-0070|FD-Score:16.20|P-value:1.00E-59 ID:SGTC_638|Compound:3970-0837|FD-Score:9.63|P-value:2.19E-22 ID:SGTC_650|Compound:1328-0195|FD-Score:3.28|P-value:5.00E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:5.23|P-value:7.57E-8 ID:SGTC_844|Compound:0330-0132|FD-Score:4.15|P-value:1.58E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:4.50|P-value:3.14E-6 ID:SGTC_897|Compound:1000-0139|FD-Score:9.98|P-value:6.12E-24 ID:SGTC_941|Compound:3237-0776|FD-Score:3.45|P-value:2.71E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.11|P-value:1.86E-5 ID:SGTC_1090|Compound:efavirenz|FD-Score:4.87|P-value:5.07E-7 ID:SGTC_1132|Compound:3456-3614|FD-Score:23.10|P-value:1.79E-119 ID:SGTC_1179|Compound:1021-1431|FD-Score:4.09|P-value:2.06E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:13.20|P-value:4.04E-40 ID:SGTC_1352|Compound:1496-0991|FD-Score:14.10|P-value:1.26E-45 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.35|P-value:3.87E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:4.07|P-value:2.20E-5 ID:SGTC_1433|Compound:4060-0023|FD-Score:8.61|P-value:2.67E-18 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.61|P-value:1.50E-4 ID:SGTC_1643|Compound:st009866|FD-Score:13.70|P-value:2.70E-43 ID:SGTC_1661|Compound:st013063|FD-Score:5.01|P-value:2.49E-7 ID:SGTC_1773|Compound:st048542|FD-Score:6.73|P-value:7.20E-12 ID:SGTC_1875|Compound:nocodazole|FD-Score:26.00|P-value:3.56E-150 ID:SGTC_1888|Compound:azatryptophan|FD-Score:3.91|P-value:4.49E-5 ID:SGTC_1912|Compound:st060209|FD-Score:-3.14|P-value:7.80E-4 ID:SGTC_1958|Compound:st076513|FD-Score:6.59|P-value:1.91E-11 ID:SGTC_2004|Compound:4023728|FD-Score:4.58|P-value:2.14E-6 ID:SGTC_2011|Compound:4012611|FD-Score:4.97|P-value:3.17E-7 ID:SGTC_2061|Compound:5245884|FD-Score:5.22|P-value:8.03E-8 ID:SGTC_2067|Compound:5224629|FD-Score:4.52|P-value:2.87E-6 ID:SGTC_2077|Compound:5212015|FD-Score:4.54|P-value:2.65E-6 ID:SGTC_2085|Compound:5307070|FD-Score:4.06|P-value:2.36E-5 ID:SGTC_2089|Compound:5281248|FD-Score:3.82|P-value:6.35E-5 ID:SGTC_2090|Compound:5301565|FD-Score:10.10|P-value:2.47E-24 ID:SGTC_2092|Compound:5303694|FD-Score:4.89|P-value:4.77E-7 ID:SGTC_2097|Compound:5376186|FD-Score:3.98|P-value:3.32E-5 ID:SGTC_2098|Compound:5376439|FD-Score:5.84|P-value:2.32E-9 ID:SGTC_2125|Compound:5246583|FD-Score:26.40|P-value:1.00E-154 ID:SGTC_2141|Compound:5621638|FD-Score:10.60|P-value:6.44E-27 ID:SGTC_2158|Compound:5657123|FD-Score:4.60|P-value:1.95E-6 ID:SGTC_2161|Compound:5662504|FD-Score:6.09|P-value:4.89E-10 ID:SGTC_2173|Compound:5763948|FD-Score:6.56|P-value:2.31E-11 ID:SGTC_2174|Compound:5790901|FD-Score:22.00|P-value:9.24E-109 ID:SGTC_2200|Compound:6600604|FD-Score:16.70|P-value:3.58E-63 ID:SGTC_2233|Compound:sulbentine|FD-Score:9.26|P-value:7.39E-21 ID:SGTC_2246|Compound:7293507|FD-Score:5.49|P-value:1.84E-8 ID:SGTC_2255|Compound:7941087|FD-Score:5.37|P-value:3.51E-8 ID:SGTC_2318|Compound:6029776|FD-Score:3.71|P-value:1.01E-4 ID:SGTC_2319|Compound:6033012|FD-Score:28.30|P-value:9.98E-178 ID:SGTC_2323|Compound:6148992|FD-Score:3.79|P-value:7.26E-5 ID:SGTC_2352|Compound:9027995|FD-Score:5.07|P-value:1.82E-7 ID:SGTC_2355|Compound:9061122|FD-Score:4.90|P-value:4.38E-7 ID:SGTC_2408|Compound:sulbentine|FD-Score:4.52|P-value:2.84E-6 ID:SGTC_2421|Compound:5690543|FD-Score:4.47|P-value:3.72E-6 ID:SGTC_2430|Compound:5571206|FD-Score:13.10|P-value:7.31E-40 ID:SGTC_2461|Compound:5755302|FD-Score:5.87|P-value:1.95E-9 ID:SGTC_2469|Compound:5655019|FD-Score:4.72|P-value:1.09E-6 ID:SGTC_2470|Compound:5664817|FD-Score:3.12|P-value:8.78E-4 ID:SGTC_2479|Compound:5617353|FD-Score:4.50|P-value:3.15E-6 ID:SGTC_2483|Compound:5729050|FD-Score:3.08|P-value:9.96E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:11.20|P-value:2.22E-29 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.76|P-value:8.06E-5 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.01|P-value:2.89E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.12|P-value:8.67E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.38|P-value:3.50E-4 ID:SGTC_2856|Compound:9022925|FD-Score:3.91|P-value:4.33E-5 ID:SGTC_2947|Compound:9072639|FD-Score:6.80|P-value:4.39E-12 ID:SGTC_2952|Compound:9073731|FD-Score:5.60|P-value:9.50E-9 ID:SGTC_3024|Compound:9090631|FD-Score:4.08|P-value:2.14E-5 ID:SGTC_3035|Compound:9091303|FD-Score:3.11|P-value:9.17E-4 ID:SGTC_3036|Compound:9090181|FD-Score:4.45|P-value:4.06E-6 ID:SGTC_3146|Compound:9095609|FD-Score:3.84|P-value:5.86E-5 ID:SGTC_3147|Compound:9097027|FD-Score:3.11|P-value:9.20E-4 ID:SGTC_3282|Compound:9108498|FD-Score:6.12|P-value:4.15E-10 ID:SGTC_3294|Compound:9122953|FD-Score:6.78|P-value:5.29E-12 ID:SGTC_3317|Compound:9138495|FD-Score:8.54|P-value:5.08E-18 ID:SGTC_12|Compound:0849-0099|FD-Score:7.81|P-value:2.27E-15 ID:SGTC_149|Compound:3970-0822|FD-Score:29.10|P-value:7.56E-188 ID:SGTC_163|Compound:1327-0036|FD-Score:7.29|P-value:1.33E-13 ID:SGTC_210|Compound:3551-0445|FD-Score:40.40|P-value:0 ID:SGTC_212|Compound:3807-4682|FD-Score:34.10|P-value:6.59E-257 ID:SGTC_229|Compound:benomyl|FD-Score:46.40|P-value:0 ID:SGTC_230|Compound:MPP+|FD-Score:5.70|P-value:5.38E-9 ID:SGTC_236|Compound:1509-0011|FD-Score:4.55|P-value:2.49E-6 ID:SGTC_286|Compound:k060-0067|FD-Score:22.80|P-value:3.27E-116 ID:SGTC_300|Compound:r073-0011|FD-Score:3.38|P-value:3.47E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:12.80|P-value:3.58E-38 ID:SGTC_376|Compound:3970-0862|FD-Score:6.66|P-value:1.15E-11 ID:SGTC_377|Compound:3970-0964|FD-Score:6.66|P-value:1.19E-11 ID:SGTC_379|Compound:3970-0972|FD-Score:10.90|P-value:2.99E-28 ID:SGTC_524|Compound:r073-0010|FD-Score:4.70|P-value:1.19E-6 ID:SGTC_589|Compound:1227-0070|FD-Score:16.20|P-value:1.00E-59 ID:SGTC_638|Compound:3970-0837|FD-Score:9.63|P-value:2.19E-22 ID:SGTC_650|Compound:1328-0195|FD-Score:3.28|P-value:5.00E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:5.23|P-value:7.57E-8 ID:SGTC_844|Compound:0330-0132|FD-Score:4.15|P-value:1.58E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:4.50|P-value:3.14E-6 ID:SGTC_897|Compound:1000-0139|FD-Score:9.98|P-value:6.12E-24 ID:SGTC_941|Compound:3237-0776|FD-Score:3.45|P-value:2.71E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.11|P-value:1.86E-5 ID:SGTC_1090|Compound:efavirenz|FD-Score:4.87|P-value:5.07E-7 ID:SGTC_1132|Compound:3456-3614|FD-Score:23.10|P-value:1.79E-119 ID:SGTC_1179|Compound:1021-1431|FD-Score:4.09|P-value:2.06E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:13.20|P-value:4.04E-40 ID:SGTC_1352|Compound:1496-0991|FD-Score:14.10|P-value:1.26E-45 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.35|P-value:3.87E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:4.07|P-value:2.20E-5 ID:SGTC_1433|Compound:4060-0023|FD-Score:8.61|P-value:2.67E-18 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.61|P-value:1.50E-4 ID:SGTC_1643|Compound:st009866|FD-Score:13.70|P-value:2.70E-43 ID:SGTC_1661|Compound:st013063|FD-Score:5.01|P-value:2.49E-7 ID:SGTC_1773|Compound:st048542|FD-Score:6.73|P-value:7.20E-12 ID:SGTC_1875|Compound:nocodazole|FD-Score:26.00|P-value:3.56E-150 ID:SGTC_1888|Compound:azatryptophan|FD-Score:3.91|P-value:4.49E-5 ID:SGTC_1912|Compound:st060209|FD-Score:-3.14|P-value:7.80E-4 ID:SGTC_1958|Compound:st076513|FD-Score:6.59|P-value:1.91E-11 ID:SGTC_2004|Compound:4023728|FD-Score:4.58|P-value:2.14E-6 ID:SGTC_2011|Compound:4012611|FD-Score:4.97|P-value:3.17E-7 ID:SGTC_2061|Compound:5245884|FD-Score:5.22|P-value:8.03E-8 ID:SGTC_2067|Compound:5224629|FD-Score:4.52|P-value:2.87E-6 ID:SGTC_2077|Compound:5212015|FD-Score:4.54|P-value:2.65E-6 ID:SGTC_2085|Compound:5307070|FD-Score:4.06|P-value:2.36E-5 ID:SGTC_2089|Compound:5281248|FD-Score:3.82|P-value:6.35E-5 ID:SGTC_2090|Compound:5301565|FD-Score:10.10|P-value:2.47E-24 ID:SGTC_2092|Compound:5303694|FD-Score:4.89|P-value:4.77E-7 ID:SGTC_2097|Compound:5376186|FD-Score:3.98|P-value:3.32E-5 ID:SGTC_2098|Compound:5376439|FD-Score:5.84|P-value:2.32E-9 ID:SGTC_2125|Compound:5246583|FD-Score:26.40|P-value:1.00E-154 ID:SGTC_2141|Compound:5621638|FD-Score:10.60|P-value:6.44E-27 ID:SGTC_2158|Compound:5657123|FD-Score:4.60|P-value:1.95E-6 ID:SGTC_2161|Compound:5662504|FD-Score:6.09|P-value:4.89E-10 ID:SGTC_2173|Compound:5763948|FD-Score:6.56|P-value:2.31E-11 ID:SGTC_2174|Compound:5790901|FD-Score:22.00|P-value:9.24E-109 ID:SGTC_2200|Compound:6600604|FD-Score:16.70|P-value:3.58E-63 ID:SGTC_2233|Compound:sulbentine|FD-Score:9.26|P-value:7.39E-21 ID:SGTC_2246|Compound:7293507|FD-Score:5.49|P-value:1.84E-8 ID:SGTC_2255|Compound:7941087|FD-Score:5.37|P-value:3.51E-8 ID:SGTC_2318|Compound:6029776|FD-Score:3.71|P-value:1.01E-4 ID:SGTC_2319|Compound:6033012|FD-Score:28.30|P-value:9.98E-178 ID:SGTC_2323|Compound:6148992|FD-Score:3.79|P-value:7.26E-5 ID:SGTC_2352|Compound:9027995|FD-Score:5.07|P-value:1.82E-7 ID:SGTC_2355|Compound:9061122|FD-Score:4.90|P-value:4.38E-7 ID:SGTC_2408|Compound:sulbentine|FD-Score:4.52|P-value:2.84E-6 ID:SGTC_2421|Compound:5690543|FD-Score:4.47|P-value:3.72E-6 ID:SGTC_2430|Compound:5571206|FD-Score:13.10|P-value:7.31E-40 ID:SGTC_2461|Compound:5755302|FD-Score:5.87|P-value:1.95E-9 ID:SGTC_2469|Compound:5655019|FD-Score:4.72|P-value:1.09E-6 ID:SGTC_2470|Compound:5664817|FD-Score:3.12|P-value:8.78E-4 ID:SGTC_2479|Compound:5617353|FD-Score:4.50|P-value:3.15E-6 ID:SGTC_2483|Compound:5729050|FD-Score:3.08|P-value:9.96E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:11.20|P-value:2.22E-29 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.76|P-value:8.06E-5 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.01|P-value:2.89E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.12|P-value:8.67E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.38|P-value:3.50E-4 ID:SGTC_2856|Compound:9022925|FD-Score:3.91|P-value:4.33E-5 ID:SGTC_2947|Compound:9072639|FD-Score:6.80|P-value:4.39E-12 ID:SGTC_2952|Compound:9073731|FD-Score:5.60|P-value:9.50E-9 ID:SGTC_3024|Compound:9090631|FD-Score:4.08|P-value:2.14E-5 ID:SGTC_3035|Compound:9091303|FD-Score:3.11|P-value:9.17E-4 ID:SGTC_3036|Compound:9090181|FD-Score:4.45|P-value:4.06E-6 ID:SGTC_3146|Compound:9095609|FD-Score:3.84|P-value:5.86E-5 ID:SGTC_3147|Compound:9097027|FD-Score:3.11|P-value:9.20E-4 ID:SGTC_3282|Compound:9108498|FD-Score:6.12|P-value:4.15E-10 ID:SGTC_3294|Compound:9122953|FD-Score:6.78|P-value:5.29E-12 ID:SGTC_3317|Compound:9138495|FD-Score:8.54|P-value:5.08E-18

Top fitness defect scores for YER007W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_229 benomyl 46.40 22.9 µM 0 tubulin folding & SWR complex 1 0
2 SGTC_210 3551-0445 40.40 56.8 µM 0 1 0
3 SGTC_212 3807-4682 34.10 38.8 µM 6.59E-257 tubulin folding & SWR complex 1 7.56E-255
4 SGTC_149 3970-0822 29.10 46.1 µM 7.56E-188 tubulin folding & SWR complex 1 2.34E-186
5 SGTC_2319 6033012 28.30 152.6 µM 9.98E-178 tubulin folding & SWR complex 2 2.55E-176
6 SGTC_2125 5246583 26.40 200.0 µM 1.00E-154 tubulin folding & SWR complex 1 1.66E-153
7 SGTC_1875 nocodazole 26.00 6.0 µM 3.56E-150 tubulin folding & SWR complex 2 5.40E-149
8 SGTC_1132 3456-3614 23.10 2.5 µM 1.79E-119 tubulin folding & SWR complex 2 1.53E-118
9 SGTC_286 k060-0067 22.80 22.9 µM 3.27E-116 tubulin folding & SWR complex 1 2.62E-115
10 SGTC_2174 5790901 22.00 200.0 µM 9.24E-109 tubulin folding & SWR complex 2 6.44E-108
11 SGTC_2200 6600604 16.70 21.9 µM 3.58E-63 tubulin folding & SWR complex 3 1.07E-62
12 SGTC_589 1227-0070 16.20 34.1 µM 1.00E-59 2 2.79E-59
13 SGTC_1352 1496-0991 14.10 99.0 µM 1.26E-45 tubulin folding & SWR complex 2 2.71E-45
14 SGTC_1643 st009866 13.70 20.2 µM 2.70E-43 5 5.57E-43
15 SGTC_1326 1431-2094 13.20 10.8 µM 4.04E-40 tubulin folding & SWR complex 3 7.85E-40
16 SGTC_2430 5571206 13.10 200.0 µM 7.31E-40 5 1.41E-39
17 SGTC_375 3970-0961 12.80 9.3 µM 3.58E-38 tubulin folding & SWR complex 1 6.72E-38
18 SGTC_2510 benzyl isothiocyanate 11.20 8.3 µM 2.22E-29 2 3.55E-29
19 SGTC_379 3970-0972 10.90 5.9 µM 2.99E-28 tubulin folding & SWR complex 1 4.68E-28
20 SGTC_2141 5621638 10.60 7.7 µM 6.44E-27 tubulin folding & SWR complex 4 9.85E-27

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.764 0 YOR349W CIN1 hom Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.701 0 YPL241C CIN2 hom GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
0.648 0 YML124C TUB3 hom Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
0.530 6.43E-243 YAL011W SWC3 hom Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
0.523 1.08E-234 YDR334W SWR1 hom Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
0.517 1.09E-228 YML041C VPS71 hom Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
0.512 1.16E-223 YDR485C VPS72 hom Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
0.505 1.08E-216 YLR085C ARP6 hom Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
0.421 4.86E-144 YBR231C SWC5 hom Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
0.371 5.70E-110 YMR138W CIN4 hom GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
0.357 3.58E-101 YOL012C HTZ1 hom Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
0.329 1.19E-85 YKL025C PAN3 hom Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.329 1.34E-85 YGL094C PAN2 hom Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.327 2.74E-84 YBL034C STU1 het Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
0.313 9.26E-77 YAL024C LTE1 hom Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures