YER008C / SEC3

Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER008C / SEC3

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:7.69|P-value:2.61E-19 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.11|P-value:1.49E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:2.95|P-value:3.27E-4 ID:SGTC_252|Compound:miconazole|FD-Score:4.47|P-value:1.14E-7 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.00|P-value:1.90E-6 ID:SGTC_312|Compound:4092-0653|FD-Score:3.98|P-value:2.07E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:3.44|P-value:3.59E-5 ID:SGTC_369|Compound:0957-0393|FD-Score:4.28|P-value:3.73E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:3.23|P-value:9.69E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:-3.57|P-value:1.61E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:2.86|P-value:4.71E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:-3.27|P-value:7.16E-5 ID:SGTC_713|Compound:1488-1073|FD-Score:3.86|P-value:4.10E-6 ID:SGTC_839|Compound:0122-0028|FD-Score:5.30|P-value:4.43E-10 ID:SGTC_854|Compound:0422-0053|FD-Score:3.16|P-value:1.31E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.36|P-value:5.25E-5 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.85|P-value:4.28E-6 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.53|P-value:2.23E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:5.50|P-value:9.66E-11 ID:SGTC_1043|Compound:0916-0009|FD-Score:2.73|P-value:8.10E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:7.48|P-value:2.35E-18 ID:SGTC_1086|Compound:cerivastatin|FD-Score:3.61|P-value:1.52E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:5.79|P-value:1.03E-11 ID:SGTC_1191|Compound:1487-1166|FD-Score:3.72|P-value:8.55E-6 ID:SGTC_1242|Compound:0402-0103|FD-Score:-2.69|P-value:8.78E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.72|P-value:8.62E-6 ID:SGTC_1308|Compound:1187-1617|FD-Score:-3.17|P-value:1.14E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:2.87|P-value:4.69E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:5.54|P-value:7.31E-11 ID:SGTC_1685|Compound:st024049|FD-Score:-3.11|P-value:1.44E-4 ID:SGTC_1697|Compound:st025554|FD-Score:2.87|P-value:4.65E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.37|P-value:4.86E-5 ID:SGTC_2034|Compound:5197015|FD-Score:2.92|P-value:3.71E-4 ID:SGTC_2094|Compound:5332512|FD-Score:2.80|P-value:6.09E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:2.70|P-value:9.07E-4 ID:SGTC_2341|Compound:7987486|FD-Score:2.71|P-value:8.68E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.02|P-value:2.44E-4 ID:SGTC_2463|Compound:5473429|FD-Score:3.74|P-value:7.63E-6 ID:SGTC_2464|Compound:5717415|FD-Score:2.97|P-value:2.98E-4 ID:SGTC_2710|Compound:st078562|FD-Score:3.12|P-value:1.60E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.35|P-value:5.50E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.79|P-value:6.07E-6 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.16|P-value:1.16E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.74|P-value:2.10E-8 ID:SGTC_2936|Compound:9038235|FD-Score:-3.48|P-value:2.61E-5 ID:SGTC_3003|Compound:9075612|FD-Score:3.08|P-value:1.90E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-4.02|P-value:1.46E-6 ID:SGTC_3152|Compound:9099253|FD-Score:4.21|P-value:5.68E-7 ID:SGTC_3207|Compound:9114163|FD-Score:3.60|P-value:1.61E-5 ID:SGTC_3294|Compound:9122953|FD-Score:-2.76|P-value:6.44E-4 ID:SGTC_3335|Compound:9143315|FD-Score:-2.88|P-value:3.96E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-2.67|P-value:9.16E-4 ID:SGTC_57|Compound:miconazole|FD-Score:7.69|P-value:2.61E-19 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.11|P-value:1.49E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:2.95|P-value:3.27E-4 ID:SGTC_252|Compound:miconazole|FD-Score:4.47|P-value:1.14E-7 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.00|P-value:1.90E-6 ID:SGTC_312|Compound:4092-0653|FD-Score:3.98|P-value:2.07E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:3.44|P-value:3.59E-5 ID:SGTC_369|Compound:0957-0393|FD-Score:4.28|P-value:3.73E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:3.23|P-value:9.69E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:-3.57|P-value:1.61E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:2.86|P-value:4.71E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:-3.27|P-value:7.16E-5 ID:SGTC_713|Compound:1488-1073|FD-Score:3.86|P-value:4.10E-6 ID:SGTC_839|Compound:0122-0028|FD-Score:5.30|P-value:4.43E-10 ID:SGTC_854|Compound:0422-0053|FD-Score:3.16|P-value:1.31E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.36|P-value:5.25E-5 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.85|P-value:4.28E-6 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.53|P-value:2.23E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:5.50|P-value:9.66E-11 ID:SGTC_1043|Compound:0916-0009|FD-Score:2.73|P-value:8.10E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:7.48|P-value:2.35E-18 ID:SGTC_1086|Compound:cerivastatin|FD-Score:3.61|P-value:1.52E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:5.79|P-value:1.03E-11 ID:SGTC_1191|Compound:1487-1166|FD-Score:3.72|P-value:8.55E-6 ID:SGTC_1242|Compound:0402-0103|FD-Score:-2.69|P-value:8.78E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.72|P-value:8.62E-6 ID:SGTC_1308|Compound:1187-1617|FD-Score:-3.17|P-value:1.14E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:2.87|P-value:4.69E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:5.54|P-value:7.31E-11 ID:SGTC_1685|Compound:st024049|FD-Score:-3.11|P-value:1.44E-4 ID:SGTC_1697|Compound:st025554|FD-Score:2.87|P-value:4.65E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.37|P-value:4.86E-5 ID:SGTC_2034|Compound:5197015|FD-Score:2.92|P-value:3.71E-4 ID:SGTC_2094|Compound:5332512|FD-Score:2.80|P-value:6.09E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:2.70|P-value:9.07E-4 ID:SGTC_2341|Compound:7987486|FD-Score:2.71|P-value:8.68E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.02|P-value:2.44E-4 ID:SGTC_2463|Compound:5473429|FD-Score:3.74|P-value:7.63E-6 ID:SGTC_2464|Compound:5717415|FD-Score:2.97|P-value:2.98E-4 ID:SGTC_2710|Compound:st078562|FD-Score:3.12|P-value:1.60E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.35|P-value:5.50E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.79|P-value:6.07E-6 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.16|P-value:1.16E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.74|P-value:2.10E-8 ID:SGTC_2936|Compound:9038235|FD-Score:-3.48|P-value:2.61E-5 ID:SGTC_3003|Compound:9075612|FD-Score:3.08|P-value:1.90E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-4.02|P-value:1.46E-6 ID:SGTC_3152|Compound:9099253|FD-Score:4.21|P-value:5.68E-7 ID:SGTC_3207|Compound:9114163|FD-Score:3.60|P-value:1.61E-5 ID:SGTC_3294|Compound:9122953|FD-Score:-2.76|P-value:6.44E-4 ID:SGTC_3335|Compound:9143315|FD-Score:-2.88|P-value:3.96E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-2.67|P-value:9.16E-4

Top fitness defect scores for YER008C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_57 miconazole 7.69 162.5 nM 2.61E-19 ergosterol depletion effects on membrane 11 7.17E-15 0.34
2 SGTC_1072 oxiconazole 7.48 14.9 nM 2.35E-18 ergosterol depletion effects on membrane 7 3.71E-14 0.66
3 SGTC_1134 3477-0009 5.79 42.5 µM 1.03E-11 4 3.50E-9 0.45
4 SGTC_1512 0259-0544 5.54 12.2 µM 7.31E-11 2 1.52E-8 0.92
5 SGTC_1039 k821-0043 5.50 94.0 µM 9.66E-11 Golgi 1 1.87E-8 1.07
6 SGTC_839 0122-0028 5.30 230.0 µM 4.43E-10 RSC complex & mRNA processing 18 5.88E-8 0.02
7 SGTC_2778 tioconazole 4.74 65.1 nM 2.10E-8 ergosterol depletion effects on membrane 21 1.07E-6 0.02
8 SGTC_252 miconazole 4.47 1.3 nM 1.14E-7 ergosterol depletion effects on membrane 13 3.83E-6 0.46
9 SGTC_369 0957-0393 4.28 4.5 µM 3.73E-7 cell wall signaling 5 9.35E-6 0.00
10 SGTC_3152 9099253 4.21 49.5 µM 5.68E-7 NEO1-PIK1 25 1.28E-5 0.02
11 SGTC_256 clotrimazole 4.00 625.0 nM 1.90E-6 ergosterol depletion effects on membrane 21 3.20E-5 0.09
12 SGTC_312 4092-0653 3.98 6.8 µM 2.07E-6 ergosterol depletion effects on membrane 31 3.40E-5 0.01
13 SGTC_713 1488-1073 3.86 324.0 µM 4.10E-6 ERG2 2 5.71E-5 0.28
14 SGTC_1020 4335-0961 3.85 93.3 µM 4.28E-6 7 5.90E-5 0.13
15 SGTC_2752 diclazuril 3.79 33.1 µM 6.07E-6 9 7.68E-5 0.07
16 SGTC_2463 5473429 3.74 200.0 µM 7.63E-6 ERG2 6 9.14E-5 0.14
17 SGTC_1191 1487-1166 3.72 10.6 µM 8.55E-6 azole & statin 4 9.96E-5 0.04
18 SGTC_1295 1013-0063 3.72 9.8 µM 8.62E-6 1 1.00E-4 0.70
19 SGTC_1086 cerivastatin 3.61 2.2 µM 1.52E-5 azole & statin 5 1.54E-4 0.12
20 SGTC_3207 9114163 3.60 49.5 µM 1.61E-5 15 1.61E-4 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.355 3.34E-100 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.319 6.40E-80 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.311 3.44E-76 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.294 9.61E-68 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.245 8.72E-47 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.203 1.16E-32 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.199 2.33E-31 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.197 1.63E-30 YAL038W CDC19 het Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
0.196 1.94E-30 YCL031C RRP7 het Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress
0.195 4.71E-30 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.181 5.33E-26 YMR277W FCP1 het Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
0.178 2.98E-25 YDR166C SEC5 het Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
0.168 1.31E-22 YGL116W CDC20 het Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
0.167 1.71E-22 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.162 4.65E-21 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data