YER012W / PRE1

Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER012W / PRE1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:5.07|P-value:6.61E-6 ID:SGTC_64|Compound:000l-0018|FD-Score:8.02|P-value:2.83E-12 ID:SGTC_90|Compound:0870-0863|FD-Score:-4.00|P-value:2.82E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:4.58|P-value:4.28E-5 ID:SGTC_127|Compound:0180-0333|FD-Score:-4.41|P-value:7.12E-5 ID:SGTC_130|Compound:0136-0219|FD-Score:4.85|P-value:1.56E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:-6.21|P-value:4.25E-8 ID:SGTC_137|Compound:0438-0332|FD-Score:23.90|P-value:3.82E-94 ID:SGTC_138|Compound:0850-0259|FD-Score:6.17|P-value:5.87E-8 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.46|P-value:1.25E-8 ID:SGTC_142|Compound:0335-0849|FD-Score:9.35|P-value:4.65E-16 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.87|P-value:4.20E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:3.93|P-value:3.68E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:10.00|P-value:3.82E-18 ID:SGTC_294|Compound:k038-0071|FD-Score:4.03|P-value:2.74E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.96|P-value:1.01E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:-4.30|P-value:1.04E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:4.57|P-value:4.32E-5 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.95|P-value:3.54E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.40|P-value:7.23E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:6.89|P-value:1.57E-9 ID:SGTC_770|Compound:0389-1121|FD-Score:4.06|P-value:2.44E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.18|P-value:3.99E-6 ID:SGTC_795|Compound:0685-0265|FD-Score:-4.06|P-value:2.26E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-3.90|P-value:3.82E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:4.34|P-value:9.82E-5 ID:SGTC_809|Compound:3998-0021|FD-Score:4.45|P-value:6.59E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:4.54|P-value:4.80E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:5.35|P-value:2.15E-6 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.65|P-value:8.22E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:-4.00|P-value:2.77E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-5.57|P-value:7.96E-7 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.92|P-value:3.81E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:-3.91|P-value:3.75E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:3.82|P-value:5.20E-4 ID:SGTC_1563|Compound:conessine|FD-Score:-4.13|P-value:1.85E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:17.00|P-value:1.23E-48 ID:SGTC_1679|Compound:4130-1276|FD-Score:-6.56|P-value:7.87E-9 ID:SGTC_1712|Compound:st032288|FD-Score:5.47|P-value:1.33E-6 ID:SGTC_2014|Compound:4013930|FD-Score:5.48|P-value:1.28E-6 ID:SGTC_2139|Compound:5578692|FD-Score:-4.62|P-value:3.36E-5 ID:SGTC_2152|Compound:5568786|FD-Score:-3.72|P-value:6.63E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.80|P-value:5.53E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.09|P-value:6.20E-6 ID:SGTC_2322|Compound:7413319|FD-Score:-3.79|P-value:5.40E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-4.33|P-value:9.50E-5 ID:SGTC_2427|Compound:5545055|FD-Score:-3.59|P-value:9.72E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:-4.54|P-value:4.48E-5 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-3.60|P-value:9.30E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.72|P-value:7.18E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:-5.59|P-value:7.29E-7 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:-4.25|P-value:1.24E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.14|P-value:1.90E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-5.76|P-value:3.43E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-4.53|P-value:4.59E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-4.86|P-value:1.37E-5 ID:SGTC_3072|Compound:9116419|FD-Score:4.54|P-value:4.93E-5 ID:SGTC_3075|Compound:9116269|FD-Score:3.62|P-value:9.60E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-4.62|P-value:3.34E-5 ID:SGTC_3133|Compound:9127736|FD-Score:5.37|P-value:2.02E-6 ID:SGTC_3142|Compound:9086498|FD-Score:4.93|P-value:1.17E-5 ID:SGTC_3212|Compound:9128307|FD-Score:4.10|P-value:2.18E-4 ID:SGTC_3247|Compound:9135149|FD-Score:3.84|P-value:4.95E-4 ID:SGTC_3353|Compound:9151644|FD-Score:-3.72|P-value:6.71E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:5.07|P-value:6.61E-6 ID:SGTC_64|Compound:000l-0018|FD-Score:8.02|P-value:2.83E-12 ID:SGTC_90|Compound:0870-0863|FD-Score:-4.00|P-value:2.82E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:4.58|P-value:4.28E-5 ID:SGTC_127|Compound:0180-0333|FD-Score:-4.41|P-value:7.12E-5 ID:SGTC_130|Compound:0136-0219|FD-Score:4.85|P-value:1.56E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:-6.21|P-value:4.25E-8 ID:SGTC_137|Compound:0438-0332|FD-Score:23.90|P-value:3.82E-94 ID:SGTC_138|Compound:0850-0259|FD-Score:6.17|P-value:5.87E-8 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.46|P-value:1.25E-8 ID:SGTC_142|Compound:0335-0849|FD-Score:9.35|P-value:4.65E-16 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.87|P-value:4.20E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:3.93|P-value:3.68E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:10.00|P-value:3.82E-18 ID:SGTC_294|Compound:k038-0071|FD-Score:4.03|P-value:2.74E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.96|P-value:1.01E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:-4.30|P-value:1.04E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:4.57|P-value:4.32E-5 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.95|P-value:3.54E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.40|P-value:7.23E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:6.89|P-value:1.57E-9 ID:SGTC_770|Compound:0389-1121|FD-Score:4.06|P-value:2.44E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.18|P-value:3.99E-6 ID:SGTC_795|Compound:0685-0265|FD-Score:-4.06|P-value:2.26E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-3.90|P-value:3.82E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:4.34|P-value:9.82E-5 ID:SGTC_809|Compound:3998-0021|FD-Score:4.45|P-value:6.59E-5 ID:SGTC_878|Compound:0828-0289|FD-Score:4.54|P-value:4.80E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:5.35|P-value:2.15E-6 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.65|P-value:8.22E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:-4.00|P-value:2.77E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-5.57|P-value:7.96E-7 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.92|P-value:3.81E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:-3.91|P-value:3.75E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:3.82|P-value:5.20E-4 ID:SGTC_1563|Compound:conessine|FD-Score:-4.13|P-value:1.85E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:17.00|P-value:1.23E-48 ID:SGTC_1679|Compound:4130-1276|FD-Score:-6.56|P-value:7.87E-9 ID:SGTC_1712|Compound:st032288|FD-Score:5.47|P-value:1.33E-6 ID:SGTC_2014|Compound:4013930|FD-Score:5.48|P-value:1.28E-6 ID:SGTC_2139|Compound:5578692|FD-Score:-4.62|P-value:3.36E-5 ID:SGTC_2152|Compound:5568786|FD-Score:-3.72|P-value:6.63E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.80|P-value:5.53E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.09|P-value:6.20E-6 ID:SGTC_2322|Compound:7413319|FD-Score:-3.79|P-value:5.40E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-4.33|P-value:9.50E-5 ID:SGTC_2427|Compound:5545055|FD-Score:-3.59|P-value:9.72E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:-4.54|P-value:4.48E-5 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-3.60|P-value:9.30E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.72|P-value:7.18E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:-5.59|P-value:7.29E-7 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:-4.25|P-value:1.24E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.14|P-value:1.90E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-5.76|P-value:3.43E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-4.53|P-value:4.59E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-4.86|P-value:1.37E-5 ID:SGTC_3072|Compound:9116419|FD-Score:4.54|P-value:4.93E-5 ID:SGTC_3075|Compound:9116269|FD-Score:3.62|P-value:9.60E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-4.62|P-value:3.34E-5 ID:SGTC_3133|Compound:9127736|FD-Score:5.37|P-value:2.02E-6 ID:SGTC_3142|Compound:9086498|FD-Score:4.93|P-value:1.17E-5 ID:SGTC_3212|Compound:9128307|FD-Score:4.10|P-value:2.18E-4 ID:SGTC_3247|Compound:9135149|FD-Score:3.84|P-value:4.95E-4 ID:SGTC_3353|Compound:9151644|FD-Score:-3.72|P-value:6.71E-4

Top fitness defect scores for YER012W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_137 0438-0332 23.90 85.4 µM 3.82E-94 ubiquinone biosynthesis & proteasome 2 1.88E-126 5.99
2 SGTC_1597 7-benzyloxygramine 17.00 47.5 µM 1.23E-48 calcium & mitochondrial duress 4 5.11E-65 0.16
3 SGTC_158 k038-0087 10.00 26.4 µM 3.82E-18 ubiquinone biosynthesis & proteasome 5 6.86E-24 0.71
4 SGTC_142 0335-0849 9.35 62.7 µM 4.65E-16 ubiquinone biosynthesis & proteasome 5 4.42E-21 1.72
5 SGTC_64 000l-0018 8.02 272.1 µM 2.83E-12 ubiquinone biosynthesis & proteasome 19 5.50E-16 0.18
6 SGTC_609 0986-0026 6.89 86.3 µM 1.57E-9 calcium & mitochondrial duress 5 2.70E-12 0.39
7 SGTC_138 0850-0259 6.17 40.9 µM 5.87E-8 cell wall 33 3.48E-10 0.08
8 SGTC_2014 4013930 5.48 200.0 µM 1.28E-6 1 2.16E-8 1.00
9 SGTC_1712 st032288 5.47 53.4 µM 1.33E-6 calcium & mitochondrial duress 18 2.28E-8 0.08
10 SGTC_3133 9127736 5.37 49.5 µM 2.02E-6 1 4.01E-8 1.56
11 SGTC_879 0831-1030 5.35 162.0 µM 2.15E-6 7 4.35E-8 0.05
12 SGTC_2250 alverine citrate 5.09 130.0 µM 6.20E-6 21 1.79E-7 0.23
13 SGTC_36 0335-0881 5.07 14.2 µM 6.61E-6 ubiquinone biosynthesis & proteasome 22 1.95E-7 0.17
14 SGTC_312 4092-0653 4.96 6.8 µM 1.01E-5 ergosterol depletion effects on membrane 20 3.45E-7 0.06
15 SGTC_3142 9086498 4.93 49.5 µM 1.17E-5 iron homeostasis 31 4.18E-7 0.10
16 SGTC_130 0136-0219 4.85 337.7 µM 1.56E-5 4 6.16E-7 0.09
17 SGTC_122 0236-0016 4.58 402.4 µM 4.28E-5 RNA processing & uracil transport 11 2.37E-6 0.01
18 SGTC_367 0335-0881 4.57 7.1 µM 4.32E-5 ubiquinone biosynthesis & proteasome 16 2.40E-6 0.08
19 SGTC_878 0828-0289 4.54 13.7 µM 4.80E-5 16 2.77E-6 0.05
20 SGTC_3072 9116419 4.54 49.5 µM 4.93E-5 ERG2 3 2.87E-6 0.41

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.490 4.75E-202 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.468 9.17E-182 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.446 7.00E-164 YOR362C PRE10 het Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.445 4.84E-163 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.414 7.32E-139 YML092C PRE8 het Alpha 2 subunit of the 20S proteasome
0.389 1.01E-121 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.388 1.16E-120 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.374 3.80E-112 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.360 6.40E-103 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.334 5.24E-88 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.318 1.14E-79 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.305 2.71E-73 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.293 1.99E-67 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.291 2.11E-66 YDL183C YDL183C hom Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
0.289 1.01E-65 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER