YER013W / PRP22

DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER013W / PRP22

Click on Significant Values for Screen Details ID:SGTC_42|Compound:0133-0086|FD-Score:2.69|P-value:9.95E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:-3.00|P-value:2.74E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-13.40|P-value:3.02E-54 ID:SGTC_180|Compound:1486-1293|FD-Score:-2.76|P-value:7.39E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.09|P-value:1.93E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:2.74|P-value:8.21E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.70|P-value:1.04E-5 ID:SGTC_331|Compound:k048-0133|FD-Score:2.84|P-value:5.54E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:2.97|P-value:3.24E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:-2.86|P-value:4.93E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.09|P-value:1.93E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.74|P-value:8.98E-6 ID:SGTC_550|Compound:4123-0385|FD-Score:3.87|P-value:4.51E-6 ID:SGTC_621|Compound:3584-0052|FD-Score:2.81|P-value:6.42E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:2.91|P-value:4.27E-4 ID:SGTC_657|Compound:3534-0529|FD-Score:-2.79|P-value:6.55E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:4.07|P-value:1.51E-6 ID:SGTC_778|Compound:3456-2434|FD-Score:-6.63|P-value:1.20E-14 ID:SGTC_912|Compound:3474-0017|FD-Score:2.94|P-value:3.72E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.92|P-value:3.26E-6 ID:SGTC_1014|Compound:4130-3005|FD-Score:2.88|P-value:4.77E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.32|P-value:6.55E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:11.50|P-value:2.65E-40 ID:SGTC_1144|Compound:3486-0183|FD-Score:-3.01|P-value:2.62E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:-3.56|P-value:2.06E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:-2.71|P-value:9.09E-4 ID:SGTC_1191|Compound:1487-1166|FD-Score:2.82|P-value:5.93E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:-2.87|P-value:4.69E-4 ID:SGTC_1275|Compound:0867-0008|FD-Score:2.87|P-value:5.03E-4 ID:SGTC_1377|Compound:2944-0135|FD-Score:-2.97|P-value:3.19E-4 ID:SGTC_1648|Compound:st011736|FD-Score:-2.90|P-value:4.19E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:2.78|P-value:7.20E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.08|P-value:2.03E-4 ID:SGTC_1893|Compound:st059353|FD-Score:2.85|P-value:5.33E-4 ID:SGTC_1909|Compound:st060837|FD-Score:3.23|P-value:1.04E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.41|P-value:4.63E-5 ID:SGTC_1999|Compound:st074831|FD-Score:3.03|P-value:2.56E-4 ID:SGTC_2033|Compound:5192550|FD-Score:-2.74|P-value:8.04E-4 ID:SGTC_2041|Compound:5107442|FD-Score:2.77|P-value:7.50E-4 ID:SGTC_2047|Compound:4141938|FD-Score:-3.28|P-value:8.08E-5 ID:SGTC_2061|Compound:5245884|FD-Score:3.97|P-value:2.58E-6 ID:SGTC_2065|Compound:5221596|FD-Score:4.39|P-value:2.28E-7 ID:SGTC_2153|Compound:5569715|FD-Score:2.76|P-value:7.59E-4 ID:SGTC_2154|Compound:5570129|FD-Score:3.17|P-value:1.35E-4 ID:SGTC_2156|Compound:5649887|FD-Score:4.25|P-value:5.17E-7 ID:SGTC_2191|Compound:5911152|FD-Score:3.53|P-value:2.54E-5 ID:SGTC_2193|Compound:5925765|FD-Score:-2.78|P-value:6.89E-4 ID:SGTC_2208|Compound:7283366|FD-Score:2.73|P-value:8.75E-4 ID:SGTC_2218|Compound:6965428|FD-Score:-3.29|P-value:7.53E-5 ID:SGTC_2337|Compound:9009472|FD-Score:3.41|P-value:4.43E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.05|P-value:2.28E-4 ID:SGTC_2415|Compound:st077333|FD-Score:4.37|P-value:2.62E-7 ID:SGTC_2441|Compound:5730946|FD-Score:3.34|P-value:6.36E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:2.71|P-value:9.52E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.37|P-value:5.36E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:2.89|P-value:4.62E-4 ID:SGTC_2526|Compound:patulin|FD-Score:4.07|P-value:1.47E-6 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.46|P-value:3.54E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.11|P-value:1.76E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-2.75|P-value:7.68E-4 ID:SGTC_2822|Compound:7994718|FD-Score:5.28|P-value:6.54E-10 ID:SGTC_2827|Compound:7993847|FD-Score:4.44|P-value:1.73E-7 ID:SGTC_2836|Compound:9010338|FD-Score:-3.42|P-value:4.13E-5 ID:SGTC_2845|Compound:9003024|FD-Score:3.52|P-value:2.65E-5 ID:SGTC_2907|Compound:7721985|FD-Score:-2.72|P-value:8.62E-4 ID:SGTC_2920|Compound:7982904|FD-Score:3.02|P-value:2.64E-4 ID:SGTC_2953|Compound:9074162|FD-Score:2.72|P-value:9.10E-4 ID:SGTC_3006|Compound:9075424|FD-Score:-2.70|P-value:9.46E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.28|P-value:8.36E-5 ID:SGTC_3016|Compound:9080866|FD-Score:3.32|P-value:6.76E-5 ID:SGTC_3043|Compound:9092303|FD-Score:5.05|P-value:3.23E-9 ID:SGTC_3065|Compound:9113888|FD-Score:3.87|P-value:4.45E-6 ID:SGTC_3073|Compound:9114972|FD-Score:3.47|P-value:3.37E-5 ID:SGTC_3078|Compound:9116413|FD-Score:3.59|P-value:1.86E-5 ID:SGTC_3148|Compound:9097893|FD-Score:2.70|P-value:9.87E-4 ID:SGTC_3159|Compound:9100096|FD-Score:3.05|P-value:2.36E-4 ID:SGTC_3240|Compound:9133527|FD-Score:2.84|P-value:5.56E-4 ID:SGTC_3249|Compound:9135183|FD-Score:2.79|P-value:6.84E-4 ID:SGTC_3252|Compound:9135871|FD-Score:2.96|P-value:3.33E-4 ID:SGTC_3342|Compound:9147791|FD-Score:3.29|P-value:7.79E-5 ID:SGTC_42|Compound:0133-0086|FD-Score:2.69|P-value:9.95E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:-3.00|P-value:2.74E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-13.40|P-value:3.02E-54 ID:SGTC_180|Compound:1486-1293|FD-Score:-2.76|P-value:7.39E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.09|P-value:1.93E-4 ID:SGTC_260|Compound:nsc-65238|FD-Score:2.74|P-value:8.21E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.70|P-value:1.04E-5 ID:SGTC_331|Compound:k048-0133|FD-Score:2.84|P-value:5.54E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:2.97|P-value:3.24E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:-2.86|P-value:4.93E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:3.09|P-value:1.93E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.74|P-value:8.98E-6 ID:SGTC_550|Compound:4123-0385|FD-Score:3.87|P-value:4.51E-6 ID:SGTC_621|Compound:3584-0052|FD-Score:2.81|P-value:6.42E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:2.91|P-value:4.27E-4 ID:SGTC_657|Compound:3534-0529|FD-Score:-2.79|P-value:6.55E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:4.07|P-value:1.51E-6 ID:SGTC_778|Compound:3456-2434|FD-Score:-6.63|P-value:1.20E-14 ID:SGTC_912|Compound:3474-0017|FD-Score:2.94|P-value:3.72E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.92|P-value:3.26E-6 ID:SGTC_1014|Compound:4130-3005|FD-Score:2.88|P-value:4.77E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:-3.32|P-value:6.55E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:11.50|P-value:2.65E-40 ID:SGTC_1144|Compound:3486-0183|FD-Score:-3.01|P-value:2.62E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:-3.56|P-value:2.06E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:-2.71|P-value:9.09E-4 ID:SGTC_1191|Compound:1487-1166|FD-Score:2.82|P-value:5.93E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:-2.87|P-value:4.69E-4 ID:SGTC_1275|Compound:0867-0008|FD-Score:2.87|P-value:5.03E-4 ID:SGTC_1377|Compound:2944-0135|FD-Score:-2.97|P-value:3.19E-4 ID:SGTC_1648|Compound:st011736|FD-Score:-2.90|P-value:4.19E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:2.78|P-value:7.20E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.08|P-value:2.03E-4 ID:SGTC_1893|Compound:st059353|FD-Score:2.85|P-value:5.33E-4 ID:SGTC_1909|Compound:st060837|FD-Score:3.23|P-value:1.04E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.41|P-value:4.63E-5 ID:SGTC_1999|Compound:st074831|FD-Score:3.03|P-value:2.56E-4 ID:SGTC_2033|Compound:5192550|FD-Score:-2.74|P-value:8.04E-4 ID:SGTC_2041|Compound:5107442|FD-Score:2.77|P-value:7.50E-4 ID:SGTC_2047|Compound:4141938|FD-Score:-3.28|P-value:8.08E-5 ID:SGTC_2061|Compound:5245884|FD-Score:3.97|P-value:2.58E-6 ID:SGTC_2065|Compound:5221596|FD-Score:4.39|P-value:2.28E-7 ID:SGTC_2153|Compound:5569715|FD-Score:2.76|P-value:7.59E-4 ID:SGTC_2154|Compound:5570129|FD-Score:3.17|P-value:1.35E-4 ID:SGTC_2156|Compound:5649887|FD-Score:4.25|P-value:5.17E-7 ID:SGTC_2191|Compound:5911152|FD-Score:3.53|P-value:2.54E-5 ID:SGTC_2193|Compound:5925765|FD-Score:-2.78|P-value:6.89E-4 ID:SGTC_2208|Compound:7283366|FD-Score:2.73|P-value:8.75E-4 ID:SGTC_2218|Compound:6965428|FD-Score:-3.29|P-value:7.53E-5 ID:SGTC_2337|Compound:9009472|FD-Score:3.41|P-value:4.43E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.05|P-value:2.28E-4 ID:SGTC_2415|Compound:st077333|FD-Score:4.37|P-value:2.62E-7 ID:SGTC_2441|Compound:5730946|FD-Score:3.34|P-value:6.36E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:2.71|P-value:9.52E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.37|P-value:5.36E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:2.89|P-value:4.62E-4 ID:SGTC_2526|Compound:patulin|FD-Score:4.07|P-value:1.47E-6 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.46|P-value:3.54E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.11|P-value:1.76E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-2.75|P-value:7.68E-4 ID:SGTC_2822|Compound:7994718|FD-Score:5.28|P-value:6.54E-10 ID:SGTC_2827|Compound:7993847|FD-Score:4.44|P-value:1.73E-7 ID:SGTC_2836|Compound:9010338|FD-Score:-3.42|P-value:4.13E-5 ID:SGTC_2845|Compound:9003024|FD-Score:3.52|P-value:2.65E-5 ID:SGTC_2907|Compound:7721985|FD-Score:-2.72|P-value:8.62E-4 ID:SGTC_2920|Compound:7982904|FD-Score:3.02|P-value:2.64E-4 ID:SGTC_2953|Compound:9074162|FD-Score:2.72|P-value:9.10E-4 ID:SGTC_3006|Compound:9075424|FD-Score:-2.70|P-value:9.46E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.28|P-value:8.36E-5 ID:SGTC_3016|Compound:9080866|FD-Score:3.32|P-value:6.76E-5 ID:SGTC_3043|Compound:9092303|FD-Score:5.05|P-value:3.23E-9 ID:SGTC_3065|Compound:9113888|FD-Score:3.87|P-value:4.45E-6 ID:SGTC_3073|Compound:9114972|FD-Score:3.47|P-value:3.37E-5 ID:SGTC_3078|Compound:9116413|FD-Score:3.59|P-value:1.86E-5 ID:SGTC_3148|Compound:9097893|FD-Score:2.70|P-value:9.87E-4 ID:SGTC_3159|Compound:9100096|FD-Score:3.05|P-value:2.36E-4 ID:SGTC_3240|Compound:9133527|FD-Score:2.84|P-value:5.56E-4 ID:SGTC_3249|Compound:9135183|FD-Score:2.79|P-value:6.84E-4 ID:SGTC_3252|Compound:9135871|FD-Score:2.96|P-value:3.33E-4 ID:SGTC_3342|Compound:9147791|FD-Score:3.29|P-value:7.79E-5

Top fitness defect scores for YER013W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1134 3477-0009 11.50 42.5 µM 2.65E-40 1 5.22E-31 3.52
2 SGTC_2822 7994718 5.28 71.4 µM 6.54E-10 2 6.40E-8 0.70
3 SGTC_3043 9092303 5.05 49.5 µM 3.23E-9 4 2.16E-7 0.99
4 SGTC_2827 7993847 4.44 71.4 µM 1.73E-7 5 4.54E-6 0.21
5 SGTC_2065 5221596 4.39 34.3 µM 2.28E-7 5 5.61E-6 0.28
6 SGTC_2415 st077333 4.37 57.7 µM 2.62E-7 12 6.25E-6 0.04
7 SGTC_2156 5649887 4.25 115.0 µM 5.17E-7 RPP1 & pyrimidine depletion 15 1.05E-5 0.22
8 SGTC_2526 patulin 4.07 37.6 µM 1.47E-6 13 2.33E-5 0.02
9 SGTC_704 1310-0270 4.07 142.0 µM 1.51E-6 5 2.39E-5 0.20
10 SGTC_2061 5245884 3.97 81.0 µM 2.58E-6 tubulin folding & SWR complex 6 3.60E-5 0.21
11 SGTC_3065 9113888 3.87 49.5 µM 4.45E-6 6 5.46E-5 0.17
12 SGTC_550 4123-0385 3.87 25.7 µM 4.51E-6 TSC3-RPN4 10 5.52E-5 0.09
13 SGTC_527 4476-4215 3.74 268.0 µM 8.98E-6 PDR1 3 9.38E-5 0.02
14 SGTC_3078 9116413 3.59 49.5 µM 1.86E-5 unfolded protein response 8 1.64E-4 0.16
15 SGTC_2191 5911152 3.53 200.0 µM 2.54E-5 4 2.09E-4 0.04
16 SGTC_2845 9003024 3.52 43.0 µM 2.65E-5 amide catabolism 19 2.15E-4 0.27
17 SGTC_3073 9114972 3.47 49.5 µM 3.37E-5 60S ribosome export 8 2.59E-4 0.10
18 SGTC_2531 methyl deoxycholate 3.46 70.1 µM 3.54E-5 11 2.69E-4 0.18
19 SGTC_2337 9009472 3.41 200.0 µM 4.43E-5 12 3.20E-4 0.02
20 SGTC_1918 oxolan-2-ylmethyl 3-bromobenzoate 3.41 70.1 µM 4.63E-5 8 3.31E-4 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.269 1.13E-56 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.211 3.52E-35 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.200 1.23E-31 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.197 1.33E-30 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.188 3.51E-28 YER136W GDI1 het GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
0.169 7.21E-23 YHR065C RRP3 het Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
0.164 1.17E-21 YFR031C SMC2 het Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
0.161 7.97E-21 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.160 1.62E-20 YPL277C_p YPL277C_p hom Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels
0.156 1.07E-19 YKL036C_d YKL036C_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
0.155 1.77E-19 YGR280C PXR1 het Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
0.154 2.25E-19 YJL173C RFA3 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
0.148 5.24E-18 YJR072C NPA3 het Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
0.148 6.68E-18 YHR166C CDC23 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.146 2.25E-17 YGR140W CBF2 het Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress