HIPHOP chemogenomics database

YER017C / AFG3

Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging

Zygosity: Homozygous strain

fixedexpanded
Profile for YER017C / AFG3

Click on Significant Values for Screen Details

Top fitness defect scores for YER017C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1742	st038235	9.82	28.9 µM	3.11E-16	mitochondrial stress	12	4.76E-23
2	SGTC_236	1509-0011	8.75	5.3 µM	2.79E-13		227	1.06E-18
3	SGTC_173	1273-0061	8.49	2.7 µM	1.32E-12	mitochondrial stress	57	1.04E-17
4	SGTC_2250	alverine citrate	7.16	130.0 µM	1.80E-9		33	4.17E-13
5	SGTC_275	trichlorophene	6.63	5.7 µM	2.24E-8	mitochondrial stress	98	1.68E-11
6	SGTC_1627	st003713	6.60	25.6 µM	2.55E-8	calcium & mitochondrial duress	22	2.02E-11
7	SGTC_87	0100-0052	6.59	35.5 µM	2.67E-8		23	2.16E-11
8	SGTC_1597	7-benzyloxygramine	6.58	47.5 µM	2.83E-8	calcium & mitochondrial duress	43	2.35E-11
9	SGTC_2626	ethyl orsellinate	6.21	100.0 µM	1.50E-7		4	2.72E-10
10	SGTC_832	4083-0019	6.09	164.0 µM	2.52E-7	translation	18	5.76E-10
11	SGTC_2533	agelasine	5.90	13.7 µM	5.49E-7	ERG2	12	1.80E-9
12	SGTC_1247	0448-0043	5.90	5.8 µM	5.55E-7	ERG2	12	1.82E-9
13	SGTC_1238	0333-0287	5.87	48.8 µM	6.20E-7	Golgi	4	2.15E-9
14	SGTC_2181	6316240	5.78	54.3 µM	9.04E-7		5	3.72E-9
15	SGTC_3193	9111931	5.56	49.5 µM	2.19E-6		4	1.35E-8
16	SGTC_1622	st003707	5.47	19.4 µM	3.06E-6	calcium & mitochondrial duress	12	2.20E-8
17	SGTC_2878	9043973	5.43	26.0 µM	3.63E-6		3	2.82E-8
18	SGTC_28	pentachloropyridine N-oxide	5.42	1.6 µM	3.73E-6	TSC3-RPN4	26	2.93E-8
19	SGTC_2183	6420625	5.42	130.5 µM	3.73E-6	mitochondrial processes	21	2.94E-8
20	SGTC_2232	chlorophetanol	5.22	41.5 µM	8.13E-6	TRP & mitochondrial translation	69	9.12E-8

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.405	1.06E-132	YMR193W	MRPL24	hom	Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.378	1.79E-114	YOL095C	HMI1	hom	Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
0.373	4.35E-111	YMR228W	MTF1	hom	Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.372	1.23E-110	YLR091W	GEP5	hom	Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine
0.364	1.10E-105	YPL040C	ISM1	hom	Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.358	8.27E-102	YMR097C	MTG1	hom	Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals
0.357	1.11E-101	YEL050C	RML2	hom	Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor
0.354	5.71E-100	YER050C	RSM18	hom	Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.354	1.02E-99	YDR298C	ATP5	hom	Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
0.352	1.38E-98	YLR149C	YLR149C	hom	Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress
0.351	1.27E-97	YDR194C	MSS116	hom	DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.338	5.57E-90	YMR287C	DSS1	hom	3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.338	3.35E-90	YHR168W	MTG2	hom	Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
0.337	8.42E-90	YGR215W	RSM27	hom	Mitochondrial ribosomal protein of the small subunit
0.336	1.48E-89	YDR332W	IRC3	hom	Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion