YER017C / AFG3

Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging

Zygosity: Homozygous strain
fixedexpanded
Profile for YER017C / AFG3

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:4.46|P-value:1.12E-4 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:5.42|P-value:3.73E-6 ID:SGTC_33|Compound:0986-0033|FD-Score:3.89|P-value:6.32E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:4.74|P-value:4.47E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:3.89|P-value:6.39E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:6.59|P-value:2.67E-8 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.91|P-value:7.48E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:8.49|P-value:1.32E-12 ID:SGTC_187|Compound:1488-2104|FD-Score:4.95|P-value:2.13E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:8.75|P-value:2.79E-13 ID:SGTC_268|Compound:camptothecin|FD-Score:-4.23|P-value:2.91E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:6.63|P-value:2.24E-8 ID:SGTC_283|Compound:0987-0079|FD-Score:4.30|P-value:1.87E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.94|P-value:6.77E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.02|P-value:5.44E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:4.40|P-value:1.37E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:-4.43|P-value:1.59E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.92|P-value:2.35E-5 ID:SGTC_502|Compound:l-744,832|FD-Score:5.09|P-value:1.29E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:6.09|P-value:2.52E-7 ID:SGTC_887|Compound:0885-0007|FD-Score:-3.87|P-value:8.46E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:4.08|P-value:3.61E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:4.26|P-value:2.12E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:-4.28|P-value:2.55E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:5.87|P-value:6.20E-7 ID:SGTC_1247|Compound:0448-0043|FD-Score:5.90|P-value:5.55E-7 ID:SGTC_1332|Compound:1480-0653|FD-Score:3.75|P-value:9.37E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:4.03|P-value:4.17E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:3.94|P-value:5.51E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:6.58|P-value:2.83E-8 ID:SGTC_1615|Compound:st002656|FD-Score:4.21|P-value:2.45E-4 ID:SGTC_1622|Compound:st003707|FD-Score:5.47|P-value:3.06E-6 ID:SGTC_1627|Compound:st003713|FD-Score:6.60|P-value:2.55E-8 ID:SGTC_1658|Compound:st012944|FD-Score:-4.64|P-value:8.08E-5 ID:SGTC_1693|Compound:xanthone|FD-Score:4.71|P-value:4.91E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.93|P-value:3.00E-5 ID:SGTC_1722|Compound:st034304|FD-Score:3.87|P-value:6.68E-4 ID:SGTC_1742|Compound:st038235|FD-Score:9.82|P-value:3.11E-16 ID:SGTC_1783|Compound:st039459|FD-Score:4.37|P-value:1.50E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-4.29|P-value:2.40E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-8.44|P-value:2.68E-12 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:-5.00|P-value:2.36E-5 ID:SGTC_1941|Compound:st076221|FD-Score:-4.13|P-value:3.90E-4 ID:SGTC_1954|Compound:st077032|FD-Score:4.11|P-value:3.36E-4 ID:SGTC_2168|Compound:5727979|FD-Score:4.26|P-value:2.10E-4 ID:SGTC_2181|Compound:6316240|FD-Score:5.78|P-value:9.04E-7 ID:SGTC_2183|Compound:6420625|FD-Score:5.42|P-value:3.73E-6 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:5.22|P-value:8.13E-6 ID:SGTC_2249|Compound:haloperidol|FD-Score:-4.66|P-value:7.51E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:7.16|P-value:1.80E-9 ID:SGTC_2307|Compound:7538926|FD-Score:-4.54|P-value:1.09E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-4.51|P-value:1.21E-4 ID:SGTC_2357|Compound:9067248|FD-Score:3.85|P-value:7.18E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:5.90|P-value:5.49E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:-10.60|P-value:2.31E-18 ID:SGTC_2584|Compound:androsterone|FD-Score:-4.32|P-value:2.24E-4 ID:SGTC_2585|Compound:alliin|FD-Score:5.12|P-value:1.17E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:-4.95|P-value:2.83E-5 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:6.21|P-value:1.50E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.16|P-value:1.01E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:-6.05|P-value:4.05E-7 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.87|P-value:3.75E-5 ID:SGTC_2853|Compound:9015042|FD-Score:4.93|P-value:2.32E-5 ID:SGTC_2854|Compound:9024559|FD-Score:3.88|P-value:6.45E-4 ID:SGTC_2861|Compound:9024595|FD-Score:-3.97|P-value:6.34E-4 ID:SGTC_2878|Compound:9043973|FD-Score:5.43|P-value:3.63E-6 ID:SGTC_2953|Compound:9074162|FD-Score:-4.22|P-value:3.02E-4 ID:SGTC_2968|Compound:9090663|FD-Score:-4.11|P-value:4.20E-4 ID:SGTC_3081|Compound:9117243|FD-Score:-3.85|P-value:8.76E-4 ID:SGTC_3193|Compound:9111931|FD-Score:5.56|P-value:2.19E-6 ID:SGTC_3315|Compound:9136395|FD-Score:4.00|P-value:4.58E-4 ID:SGTC_3319|Compound:9138450|FD-Score:-5.14|P-value:1.44E-5 ID:SGTC_3323|Compound:9138564|FD-Score:4.35|P-value:1.60E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:4.46|P-value:1.12E-4 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:5.42|P-value:3.73E-6 ID:SGTC_33|Compound:0986-0033|FD-Score:3.89|P-value:6.32E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:4.74|P-value:4.47E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:3.89|P-value:6.39E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:6.59|P-value:2.67E-8 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.91|P-value:7.48E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:8.49|P-value:1.32E-12 ID:SGTC_187|Compound:1488-2104|FD-Score:4.95|P-value:2.13E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:8.75|P-value:2.79E-13 ID:SGTC_268|Compound:camptothecin|FD-Score:-4.23|P-value:2.91E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:6.63|P-value:2.24E-8 ID:SGTC_283|Compound:0987-0079|FD-Score:4.30|P-value:1.87E-4 ID:SGTC_293|Compound:4049-0273|FD-Score:-3.94|P-value:6.77E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.02|P-value:5.44E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:4.40|P-value:1.37E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:-4.43|P-value:1.59E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.92|P-value:2.35E-5 ID:SGTC_502|Compound:l-744,832|FD-Score:5.09|P-value:1.29E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:6.09|P-value:2.52E-7 ID:SGTC_887|Compound:0885-0007|FD-Score:-3.87|P-value:8.46E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:4.08|P-value:3.61E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:4.26|P-value:2.12E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:-4.28|P-value:2.55E-4 ID:SGTC_1238|Compound:0333-0287|FD-Score:5.87|P-value:6.20E-7 ID:SGTC_1247|Compound:0448-0043|FD-Score:5.90|P-value:5.55E-7 ID:SGTC_1332|Compound:1480-0653|FD-Score:3.75|P-value:9.37E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:4.03|P-value:4.17E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:3.94|P-value:5.51E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:6.58|P-value:2.83E-8 ID:SGTC_1615|Compound:st002656|FD-Score:4.21|P-value:2.45E-4 ID:SGTC_1622|Compound:st003707|FD-Score:5.47|P-value:3.06E-6 ID:SGTC_1627|Compound:st003713|FD-Score:6.60|P-value:2.55E-8 ID:SGTC_1658|Compound:st012944|FD-Score:-4.64|P-value:8.08E-5 ID:SGTC_1693|Compound:xanthone|FD-Score:4.71|P-value:4.91E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.93|P-value:3.00E-5 ID:SGTC_1722|Compound:st034304|FD-Score:3.87|P-value:6.68E-4 ID:SGTC_1742|Compound:st038235|FD-Score:9.82|P-value:3.11E-16 ID:SGTC_1783|Compound:st039459|FD-Score:4.37|P-value:1.50E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-4.29|P-value:2.40E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-8.44|P-value:2.68E-12 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:-5.00|P-value:2.36E-5 ID:SGTC_1941|Compound:st076221|FD-Score:-4.13|P-value:3.90E-4 ID:SGTC_1954|Compound:st077032|FD-Score:4.11|P-value:3.36E-4 ID:SGTC_2168|Compound:5727979|FD-Score:4.26|P-value:2.10E-4 ID:SGTC_2181|Compound:6316240|FD-Score:5.78|P-value:9.04E-7 ID:SGTC_2183|Compound:6420625|FD-Score:5.42|P-value:3.73E-6 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:5.22|P-value:8.13E-6 ID:SGTC_2249|Compound:haloperidol|FD-Score:-4.66|P-value:7.51E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:7.16|P-value:1.80E-9 ID:SGTC_2307|Compound:7538926|FD-Score:-4.54|P-value:1.09E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-4.51|P-value:1.21E-4 ID:SGTC_2357|Compound:9067248|FD-Score:3.85|P-value:7.18E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:5.90|P-value:5.49E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:-10.60|P-value:2.31E-18 ID:SGTC_2584|Compound:androsterone|FD-Score:-4.32|P-value:2.24E-4 ID:SGTC_2585|Compound:alliin|FD-Score:5.12|P-value:1.17E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:-4.95|P-value:2.83E-5 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:6.21|P-value:1.50E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.16|P-value:1.01E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:-6.05|P-value:4.05E-7 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.87|P-value:3.75E-5 ID:SGTC_2853|Compound:9015042|FD-Score:4.93|P-value:2.32E-5 ID:SGTC_2854|Compound:9024559|FD-Score:3.88|P-value:6.45E-4 ID:SGTC_2861|Compound:9024595|FD-Score:-3.97|P-value:6.34E-4 ID:SGTC_2878|Compound:9043973|FD-Score:5.43|P-value:3.63E-6 ID:SGTC_2953|Compound:9074162|FD-Score:-4.22|P-value:3.02E-4 ID:SGTC_2968|Compound:9090663|FD-Score:-4.11|P-value:4.20E-4 ID:SGTC_3081|Compound:9117243|FD-Score:-3.85|P-value:8.76E-4 ID:SGTC_3193|Compound:9111931|FD-Score:5.56|P-value:2.19E-6 ID:SGTC_3315|Compound:9136395|FD-Score:4.00|P-value:4.58E-4 ID:SGTC_3319|Compound:9138450|FD-Score:-5.14|P-value:1.44E-5 ID:SGTC_3323|Compound:9138564|FD-Score:4.35|P-value:1.60E-4

Top fitness defect scores for YER017C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1742 st038235 9.82 28.9 µM 3.11E-16 mitochondrial stress 12 4.76E-23
2 SGTC_236 1509-0011 8.75 5.3 µM 2.79E-13 227 1.06E-18
3 SGTC_173 1273-0061 8.49 2.7 µM 1.32E-12 mitochondrial stress 57 1.04E-17
4 SGTC_2250 alverine citrate 7.16 130.0 µM 1.80E-9 33 4.17E-13
5 SGTC_275 trichlorophene 6.63 5.7 µM 2.24E-8 mitochondrial stress 98 1.68E-11
6 SGTC_1627 st003713 6.60 25.6 µM 2.55E-8 calcium & mitochondrial duress 22 2.02E-11
7 SGTC_87 0100-0052 6.59 35.5 µM 2.67E-8 23 2.16E-11
8 SGTC_1597 7-benzyloxygramine 6.58 47.5 µM 2.83E-8 calcium & mitochondrial duress 43 2.35E-11
9 SGTC_2626 ethyl orsellinate 6.21 100.0 µM 1.50E-7 4 2.72E-10
10 SGTC_832 4083-0019 6.09 164.0 µM 2.52E-7 translation 18 5.76E-10
11 SGTC_2533 agelasine 5.90 13.7 µM 5.49E-7 ERG2 12 1.80E-9
12 SGTC_1247 0448-0043 5.90 5.8 µM 5.55E-7 ERG2 12 1.82E-9
13 SGTC_1238 0333-0287 5.87 48.8 µM 6.20E-7 Golgi 4 2.15E-9
14 SGTC_2181 6316240 5.78 54.3 µM 9.04E-7 5 3.72E-9
15 SGTC_3193 9111931 5.56 49.5 µM 2.19E-6 4 1.35E-8
16 SGTC_1622 st003707 5.47 19.4 µM 3.06E-6 calcium & mitochondrial duress 12 2.20E-8
17 SGTC_2878 9043973 5.43 26.0 µM 3.63E-6 3 2.82E-8
18 SGTC_28 pentachloropyridine N-oxide 5.42 1.6 µM 3.73E-6 TSC3-RPN4 26 2.93E-8
19 SGTC_2183 6420625 5.42 130.5 µM 3.73E-6 mitochondrial processes 21 2.94E-8
20 SGTC_2232 chlorophetanol 5.22 41.5 µM 8.13E-6 TRP & mitochondrial translation 69 9.12E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.405 1.06E-132 YMR193W MRPL24 hom Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.378 1.79E-114 YOL095C HMI1 hom Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
0.373 4.35E-111 YMR228W MTF1 hom Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.372 1.23E-110 YLR091W GEP5 hom Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine
0.364 1.10E-105 YPL040C ISM1 hom Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.358 8.27E-102 YMR097C MTG1 hom Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals
0.357 1.11E-101 YEL050C RML2 hom Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor
0.354 5.71E-100 YER050C RSM18 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.354 1.02E-99 YDR298C ATP5 hom Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
0.352 1.38E-98 YLR149C YLR149C hom Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress
0.351 1.27E-97 YDR194C MSS116 hom DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.338 5.57E-90 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.338 3.35E-90 YHR168W MTG2 hom Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
0.337 8.42E-90 YGR215W RSM27 hom Mitochondrial ribosomal protein of the small subunit
0.336 1.48E-89 YDR332W IRC3 hom Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion