YER020W / GPA2

Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery

Zygosity: Homozygous strain
fixedexpanded
Profile for YER020W / GPA2

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:4.96|P-value:5.05E-7 ID:SGTC_36|Compound:0335-0881|FD-Score:3.12|P-value:9.70E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:3.26|P-value:6.08E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:-4.24|P-value:2.77E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:3.28|P-value:5.60E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:3.84|P-value:7.16E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:-4.80|P-value:2.41E-6 ID:SGTC_176|Compound:1486-1328|FD-Score:3.89|P-value:5.97E-5 ID:SGTC_384|Compound:k007-0144|FD-Score:5.34|P-value:7.45E-8 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-3.36|P-value:7.59E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:4.06|P-value:3.01E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:4.35|P-value:8.58E-6 ID:SGTC_528|Compound:3492-0019|FD-Score:-3.41|P-value:6.32E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:5.40|P-value:5.52E-8 ID:SGTC_568|Compound:1193-0097|FD-Score:5.41|P-value:5.26E-8 ID:SGTC_598|Compound:3937-0236|FD-Score:-3.40|P-value:6.62E-4 ID:SGTC_654|Compound:1124-0271|FD-Score:-3.59|P-value:3.35E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:5.32|P-value:8.35E-8 ID:SGTC_797|Compound:k781-0471|FD-Score:3.13|P-value:9.17E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:4.11|P-value:2.39E-5 ID:SGTC_887|Compound:0885-0007|FD-Score:3.21|P-value:7.04E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:3.19|P-value:7.68E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:3.48|P-value:2.77E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-4.12|P-value:4.65E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.12|P-value:2.35E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:-3.39|P-value:6.79E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:3.65|P-value:1.49E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.17|P-value:8.26E-4 ID:SGTC_1444|Compound:4112-3581|FD-Score:3.38|P-value:4.01E-4 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.44|P-value:5.64E-4 ID:SGTC_1616|Compound:st002734|FD-Score:6.08|P-value:1.15E-9 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.14|P-value:8.91E-4 ID:SGTC_1860|Compound:st056228|FD-Score:3.17|P-value:8.26E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.58|P-value:1.92E-4 ID:SGTC_2007|Compound:4011539|FD-Score:-4.33|P-value:1.98E-5 ID:SGTC_2011|Compound:4012611|FD-Score:3.24|P-value:6.50E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.46|P-value:3.01E-4 ID:SGTC_2052|Compound:5265182|FD-Score:3.18|P-value:7.82E-4 ID:SGTC_2061|Compound:5245884|FD-Score:4.16|P-value:1.94E-5 ID:SGTC_2242|Compound:6630449|FD-Score:-3.54|P-value:4.08E-4 ID:SGTC_2311|Compound:7694451|FD-Score:3.44|P-value:3.19E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.70|P-value:2.33E-4 ID:SGTC_2347|Compound:9049783|FD-Score:-3.91|P-value:1.05E-4 ID:SGTC_2382|Compound:9035899|FD-Score:3.80|P-value:8.28E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.32|P-value:1.02E-5 ID:SGTC_2411|Compound:st077133|FD-Score:3.42|P-value:3.45E-4 ID:SGTC_2435|Compound:5706476|FD-Score:3.26|P-value:6.03E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.50|P-value:2.60E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-3.35|P-value:7.85E-4 ID:SGTC_2809|Compound:7964133|FD-Score:4.06|P-value:2.99E-5 ID:SGTC_2982|Compound:9019404|FD-Score:-3.48|P-value:5.08E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-3.94|P-value:9.21E-5 ID:SGTC_3311|Compound:9131146|FD-Score:-6.06|P-value:3.45E-9 ID:SGTC_3|Compound:0833-0587|FD-Score:4.96|P-value:5.05E-7 ID:SGTC_36|Compound:0335-0881|FD-Score:3.12|P-value:9.70E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:3.26|P-value:6.08E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:-4.24|P-value:2.77E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:3.28|P-value:5.60E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:3.84|P-value:7.16E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:-4.80|P-value:2.41E-6 ID:SGTC_176|Compound:1486-1328|FD-Score:3.89|P-value:5.97E-5 ID:SGTC_384|Compound:k007-0144|FD-Score:5.34|P-value:7.45E-8 ID:SGTC_409|Compound:arachidonic acid|FD-Score:-3.36|P-value:7.59E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:4.06|P-value:3.01E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:4.35|P-value:8.58E-6 ID:SGTC_528|Compound:3492-0019|FD-Score:-3.41|P-value:6.32E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:5.40|P-value:5.52E-8 ID:SGTC_568|Compound:1193-0097|FD-Score:5.41|P-value:5.26E-8 ID:SGTC_598|Compound:3937-0236|FD-Score:-3.40|P-value:6.62E-4 ID:SGTC_654|Compound:1124-0271|FD-Score:-3.59|P-value:3.35E-4 ID:SGTC_668|Compound:1682-0499|FD-Score:5.32|P-value:8.35E-8 ID:SGTC_797|Compound:k781-0471|FD-Score:3.13|P-value:9.17E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:4.11|P-value:2.39E-5 ID:SGTC_887|Compound:0885-0007|FD-Score:3.21|P-value:7.04E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:3.19|P-value:7.68E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:3.48|P-value:2.77E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-4.12|P-value:4.65E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.12|P-value:2.35E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:-3.39|P-value:6.79E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:3.65|P-value:1.49E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.17|P-value:8.26E-4 ID:SGTC_1444|Compound:4112-3581|FD-Score:3.38|P-value:4.01E-4 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.44|P-value:5.64E-4 ID:SGTC_1616|Compound:st002734|FD-Score:6.08|P-value:1.15E-9 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.14|P-value:8.91E-4 ID:SGTC_1860|Compound:st056228|FD-Score:3.17|P-value:8.26E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.58|P-value:1.92E-4 ID:SGTC_2007|Compound:4011539|FD-Score:-4.33|P-value:1.98E-5 ID:SGTC_2011|Compound:4012611|FD-Score:3.24|P-value:6.50E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.46|P-value:3.01E-4 ID:SGTC_2052|Compound:5265182|FD-Score:3.18|P-value:7.82E-4 ID:SGTC_2061|Compound:5245884|FD-Score:4.16|P-value:1.94E-5 ID:SGTC_2242|Compound:6630449|FD-Score:-3.54|P-value:4.08E-4 ID:SGTC_2311|Compound:7694451|FD-Score:3.44|P-value:3.19E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-3.70|P-value:2.33E-4 ID:SGTC_2347|Compound:9049783|FD-Score:-3.91|P-value:1.05E-4 ID:SGTC_2382|Compound:9035899|FD-Score:3.80|P-value:8.28E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.32|P-value:1.02E-5 ID:SGTC_2411|Compound:st077133|FD-Score:3.42|P-value:3.45E-4 ID:SGTC_2435|Compound:5706476|FD-Score:3.26|P-value:6.03E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.50|P-value:2.60E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-3.35|P-value:7.85E-4 ID:SGTC_2809|Compound:7964133|FD-Score:4.06|P-value:2.99E-5 ID:SGTC_2982|Compound:9019404|FD-Score:-3.48|P-value:5.08E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-3.94|P-value:9.21E-5 ID:SGTC_3311|Compound:9131146|FD-Score:-6.06|P-value:3.45E-9

Top fitness defect scores for YER020W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1616 st002734 6.08 56.1 µM 1.15E-9 8 5.83E-10
2 SGTC_568 1193-0097 5.41 7.5 µM 5.26E-8 ERAD & cell cycle 74 3.22E-8
3 SGTC_542 3381-0244 5.40 89.3 µM 5.52E-8 16 3.39E-8
4 SGTC_384 k007-0144 5.34 5.2 µM 7.45E-8 ERAD & cell cycle 32 4.64E-8
5 SGTC_668 1682-0499 5.32 62.6 µM 8.35E-8 5 5.23E-8
6 SGTC_3 0833-0587 4.96 95.6 µM 5.05E-7 redox potentiating 14 3.45E-7
7 SGTC_435 c-PAF 4.35 6.7 µM 8.58E-6 28 6.67E-6
8 SGTC_2405 ticlatone 4.32 3.5 µM 1.02E-5 57 7.97E-6
9 SGTC_2061 5245884 4.16 81.0 µM 1.94E-5 tubulin folding & SWR complex 17 1.56E-5
10 SGTC_1161 k007-0610 4.12 110.0 µM 2.35E-5 ERAD & cell cycle 113 1.91E-5
11 SGTC_886 0866-0150 4.11 153.0 µM 2.39E-5 20 1.94E-5
12 SGTC_2809 7964133 4.06 13.0 µM 2.99E-5 60S ribosome export 36 2.45E-5
13 SGTC_434 1-hexadecyl-2-methylglycero-3 PC 4.06 8.0 µM 3.01E-5 24 2.47E-5
14 SGTC_176 1486-1328 3.89 72.8 µM 5.97E-5 54 5.04E-5
15 SGTC_127 0180-0333 3.84 59.1 µM 7.16E-5 mitochondrial response to ROS 51 6.10E-5
16 SGTC_2382 9035899 3.80 200.0 µM 8.28E-5 60S ribosome export 17 7.09E-5
17 SGTC_1270 0831-1170 3.65 17.3 µM 1.49E-4 15 1.31E-4
18 SGTC_1912 st060209 3.58 90.8 µM 1.92E-4 42 1.70E-4
19 SGTC_2566 crystal violet carbinol 3.50 226.0 nM 2.60E-4 55 2.33E-4
20 SGTC_1026 k007-1105 3.48 30.6 µM 2.77E-4 ERAD & cell cycle 120 2.49E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.173 6.92E-24 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.165 6.86E-22 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.162 3.55E-21 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.141 2.35E-16 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.140 3.45E-16 YLR310C CDC25 het Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
0.140 4.85E-16 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.138 1.17E-15 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.123 6.99E-13 YDL035C GPR1 hom Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis
0.120 2.76E-12 YFR008W FAR7 hom Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
0.119 4.05E-12 YPR153W YPR153W hom Putative protein of unknown function
0.119 4.42E-12 YKR014C YPT52 hom Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress
0.118 5.71E-12 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.114 3.56E-11 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.113 4.72E-11 YDL100C GET3 hom Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
0.113 5.21E-11 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA