YER023W / PRO3

Delta 1-pyrroline-5-carboxylate reductase, catalyzes the last step in proline biosynthesis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER023W / PRO3

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:3.40|P-value:3.45E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:3.41|P-value:3.17E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:-2.90|P-value:3.84E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-3.02|P-value:2.27E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:-2.71|P-value:8.30E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:3.39|P-value:3.59E-5 ID:SGTC_182|Compound:3448-4371|FD-Score:3.06|P-value:1.69E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:-2.80|P-value:5.78E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.04|P-value:1.87E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:-2.75|P-value:7.32E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.96|P-value:4.13E-9 ID:SGTC_836|Compound:0330-0104|FD-Score:-2.73|P-value:7.83E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:-3.07|P-value:1.87E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.80|P-value:4.31E-6 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.08|P-value:1.77E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:4.13|P-value:6.80E-7 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.25|P-value:6.97E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:-3.28|P-value:7.17E-5 ID:SGTC_1136|Compound:pergolide|FD-Score:3.94|P-value:2.04E-6 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.34|P-value:5.30E-5 ID:SGTC_1153|Compound:4055-0057|FD-Score:-2.70|P-value:8.66E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:4.31|P-value:2.29E-7 ID:SGTC_1247|Compound:0448-0043|FD-Score:2.83|P-value:4.55E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:4.38|P-value:1.45E-7 ID:SGTC_1290|Compound:0986-0249|FD-Score:-2.87|P-value:4.40E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.64|P-value:1.03E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.02|P-value:2.03E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:5.26|P-value:3.93E-10 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.09|P-value:1.49E-4 ID:SGTC_1603|Compound:st002045|FD-Score:2.82|P-value:4.77E-4 ID:SGTC_1609|Compound:st001574|FD-Score:-3.76|P-value:6.51E-6 ID:SGTC_1740|Compound:st038286|FD-Score:-3.81|P-value:4.83E-6 ID:SGTC_1744|Compound:st037937|FD-Score:4.73|P-value:1.58E-8 ID:SGTC_1746|Compound:st038117|FD-Score:4.86|P-value:6.52E-9 ID:SGTC_1857|Compound:st056007|FD-Score:-2.85|P-value:4.75E-4 ID:SGTC_1893|Compound:st059353|FD-Score:2.81|P-value:4.88E-4 ID:SGTC_1909|Compound:st060837|FD-Score:2.82|P-value:4.68E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-3.09|P-value:1.69E-4 ID:SGTC_2310|Compound:7668677|FD-Score:2.65|P-value:9.37E-4 ID:SGTC_2396|Compound:5342950|FD-Score:-2.88|P-value:4.29E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-3.51|P-value:2.33E-5 ID:SGTC_2445|Compound:5742597|FD-Score:3.11|P-value:1.31E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.41|P-value:3.22E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:3.31|P-value:5.23E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-8.29|P-value:2.64E-22 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.30|P-value:5.46E-5 ID:SGTC_2829|Compound:7992743|FD-Score:2.68|P-value:8.47E-4 ID:SGTC_2868|Compound:9035608|FD-Score:-2.68|P-value:9.32E-4 ID:SGTC_3168|Compound:9103419|FD-Score:3.62|P-value:1.15E-5 ID:SGTC_3171|Compound:9103192|FD-Score:-3.38|P-value:4.36E-5 ID:SGTC_3215|Compound:9129942|FD-Score:2.65|P-value:9.51E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:3.40|P-value:3.45E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:3.41|P-value:3.17E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:-2.90|P-value:3.84E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-3.02|P-value:2.27E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:-2.71|P-value:8.30E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:3.39|P-value:3.59E-5 ID:SGTC_182|Compound:3448-4371|FD-Score:3.06|P-value:1.69E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:-2.80|P-value:5.78E-4 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:3.04|P-value:1.87E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:-2.75|P-value:7.32E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-4.96|P-value:4.13E-9 ID:SGTC_836|Compound:0330-0104|FD-Score:-2.73|P-value:7.83E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:-3.07|P-value:1.87E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.80|P-value:4.31E-6 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.08|P-value:1.77E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:4.13|P-value:6.80E-7 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.25|P-value:6.97E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:-3.28|P-value:7.17E-5 ID:SGTC_1136|Compound:pergolide|FD-Score:3.94|P-value:2.04E-6 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.34|P-value:5.30E-5 ID:SGTC_1153|Compound:4055-0057|FD-Score:-2.70|P-value:8.66E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:4.31|P-value:2.29E-7 ID:SGTC_1247|Compound:0448-0043|FD-Score:2.83|P-value:4.55E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:4.38|P-value:1.45E-7 ID:SGTC_1290|Compound:0986-0249|FD-Score:-2.87|P-value:4.40E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.64|P-value:1.03E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.02|P-value:2.03E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:5.26|P-value:3.93E-10 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.09|P-value:1.49E-4 ID:SGTC_1603|Compound:st002045|FD-Score:2.82|P-value:4.77E-4 ID:SGTC_1609|Compound:st001574|FD-Score:-3.76|P-value:6.51E-6 ID:SGTC_1740|Compound:st038286|FD-Score:-3.81|P-value:4.83E-6 ID:SGTC_1744|Compound:st037937|FD-Score:4.73|P-value:1.58E-8 ID:SGTC_1746|Compound:st038117|FD-Score:4.86|P-value:6.52E-9 ID:SGTC_1857|Compound:st056007|FD-Score:-2.85|P-value:4.75E-4 ID:SGTC_1893|Compound:st059353|FD-Score:2.81|P-value:4.88E-4 ID:SGTC_1909|Compound:st060837|FD-Score:2.82|P-value:4.68E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-3.09|P-value:1.69E-4 ID:SGTC_2310|Compound:7668677|FD-Score:2.65|P-value:9.37E-4 ID:SGTC_2396|Compound:5342950|FD-Score:-2.88|P-value:4.29E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-3.51|P-value:2.33E-5 ID:SGTC_2445|Compound:5742597|FD-Score:3.11|P-value:1.31E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.41|P-value:3.22E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:3.31|P-value:5.23E-5 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-8.29|P-value:2.64E-22 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.30|P-value:5.46E-5 ID:SGTC_2829|Compound:7992743|FD-Score:2.68|P-value:8.47E-4 ID:SGTC_2868|Compound:9035608|FD-Score:-2.68|P-value:9.32E-4 ID:SGTC_3168|Compound:9103419|FD-Score:3.62|P-value:1.15E-5 ID:SGTC_3171|Compound:9103192|FD-Score:-3.38|P-value:4.36E-5 ID:SGTC_3215|Compound:9129942|FD-Score:2.65|P-value:9.51E-4

Top fitness defect scores for YER023W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1379 2144-0755 5.26 36.5 µM 3.93E-10 1 7.35E-8 0.44
2 SGTC_1746 st038117 4.86 42.0 µM 6.52E-9 3 5.86E-7 0.69
3 SGTC_1744 st037937 4.73 38.9 µM 1.58E-8 3 1.13E-6 0.40
4 SGTC_1251 0490-5699 4.38 265.0 µM 1.45E-7 5 5.83E-6 0.02
5 SGTC_1246 0422-0085 4.31 105.0 µM 2.29E-7 6 8.21E-6 0.07
6 SGTC_968 1309-0633 4.13 340.0 µM 6.80E-7 4 1.84E-5 0.25
7 SGTC_1136 pergolide 3.94 1.1 µM 2.04E-6 18 4.15E-5 0.04
8 SGTC_942 3253-1115 3.80 227.0 µM 4.31E-6 7 7.25E-5 0.06
9 SGTC_1370 2001-0092 3.64 44.6 µM 1.03E-5 7 1.39E-4 0.06
10 SGTC_3168 9103419 3.62 49.5 µM 1.15E-5 60S ribosome export 5 1.50E-4 0.82
11 SGTC_65 0798-0905 3.41 35.0 µM 3.17E-5 calcium & mitochondrial duress 13 3.20E-4 0.11
12 SGTC_2527 2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone 3.41 63.6 µM 3.22E-5 17 3.23E-4 0.05
13 SGTC_39 0986-0283 3.40 58.4 µM 3.45E-5 calcium & mitochondrial duress 14 3.41E-4 0.23
14 SGTC_179 1273-0063 3.39 6.8 µM 3.59E-5 8 3.51E-4 0.04
15 SGTC_2532 xanthone 3.31 78.9 µM 5.23E-5 6 4.64E-4 0.14
16 SGTC_2629 bisabolol 3.30 100.0 µM 5.46E-5 16 4.80E-4 0.00
17 SGTC_1034 k072-0165 3.25 12.7 µM 6.97E-5 Golgi 12 5.76E-4 0.03
18 SGTC_2445 5742597 3.11 35.6 µM 1.31E-4 18 9.20E-4 0.01
19 SGTC_1506 4226-2149 3.09 212.0 µM 1.49E-4 12 0.00101 0.02
20 SGTC_182 3448-4371 3.06 550.5 µM 1.69E-4 9 0.00111 0.18

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.193 1.82E-29 YDR044W HEM13 het Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)
0.159 1.77E-20 YNL181W_p YNL181W_p het Putative oxidoreductase; required for cell viability
0.151 1.36E-18 YKL022C CDC16 het Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation
0.148 6.68E-18 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.140 3.07E-16 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.133 9.85E-15 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.131 2.33E-14 YOR010C TIR2 hom Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis
0.128 1.16E-13 YGR041W BUD9 hom Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole
0.127 1.45E-13 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.125 4.11E-13 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.115 2.34E-11 YLR224W_p YLR224W_p hom F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene
0.112 6.61E-11 YPR156C TPO3 hom Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication
0.109 2.12E-10 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.107 4.93E-10 YLR023C IZH3 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
0.107 5.49E-10 YJL203W PRP21 het Subunit of the SF3a splicing factor complex, required for spliceosome assembly