YER025W / GCD11

Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER025W / GCD11

Click on Significant Values for Screen Details ID:SGTC_70|Compound:0141-0289|FD-Score:-5.50|P-value:9.40E-7 ID:SGTC_87|Compound:0100-0052|FD-Score:4.07|P-value:1.40E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-8.29|P-value:2.72E-13 ID:SGTC_119|Compound:0121-0043|FD-Score:-5.94|P-value:1.29E-7 ID:SGTC_124|Compound:0136-0217|FD-Score:-5.10|P-value:5.08E-6 ID:SGTC_132|Compound:0417-1715|FD-Score:4.42|P-value:4.17E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:5.80|P-value:1.34E-7 ID:SGTC_163|Compound:1327-0036|FD-Score:6.04|P-value:4.25E-8 ID:SGTC_186|Compound:3455-3465|FD-Score:-4.20|P-value:1.45E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.55|P-value:7.47E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:6.43|P-value:6.10E-9 ID:SGTC_330|Compound:1488-0319|FD-Score:5.48|P-value:5.62E-7 ID:SGTC_350|Compound:k064-0020|FD-Score:-5.47|P-value:1.07E-6 ID:SGTC_393|Compound:acivicin|FD-Score:-4.82|P-value:1.52E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:3.76|P-value:3.89E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:-4.43|P-value:6.44E-5 ID:SGTC_621|Compound:3584-0052|FD-Score:-4.89|P-value:1.15E-5 ID:SGTC_667|Compound:1498-1044|FD-Score:3.57|P-value:6.97E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:-3.95|P-value:3.35E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.14|P-value:1.09E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.79|P-value:3.55E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:3.47|P-value:9.41E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.45|P-value:3.71E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:4.74|P-value:1.23E-5 ID:SGTC_855|Compound:0428-0020|FD-Score:3.61|P-value:6.34E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:4.49|P-value:3.16E-5 ID:SGTC_894|Compound:0960-0238|FD-Score:5.53|P-value:4.52E-7 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.90|P-value:3.86E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-3.76|P-value:5.90E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.53|P-value:8.04E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-4.25|P-value:1.21E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:6.16|P-value:2.28E-8 ID:SGTC_1250|Compound:0485-0349|FD-Score:3.83|P-value:3.13E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:3.72|P-value:4.39E-4 ID:SGTC_1466|Compound:k213-0079|FD-Score:5.24|P-value:1.58E-6 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.97|P-value:1.99E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.20|P-value:8.96E-5 ID:SGTC_1766|Compound:st045414|FD-Score:8.77|P-value:4.47E-15 ID:SGTC_1772|Compound:st048439|FD-Score:3.77|P-value:3.85E-4 ID:SGTC_1797|Compound:st048695|FD-Score:-4.64|P-value:2.98E-5 ID:SGTC_1834|Compound:st055981|FD-Score:3.47|P-value:9.49E-4 ID:SGTC_1886|Compound:st057356|FD-Score:-3.90|P-value:3.81E-4 ID:SGTC_2013|Compound:4024628|FD-Score:-3.86|P-value:4.34E-4 ID:SGTC_2022|Compound:5136654|FD-Score:3.59|P-value:6.67E-4 ID:SGTC_2057|Compound:5236571|FD-Score:4.12|P-value:1.17E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.48|P-value:9.17E-4 ID:SGTC_2137|Compound:5261020|FD-Score:-3.93|P-value:3.52E-4 ID:SGTC_2156|Compound:5649887|FD-Score:5.13|P-value:2.52E-6 ID:SGTC_2248|Compound:tofa|FD-Score:-4.63|P-value:3.08E-5 ID:SGTC_2255|Compound:7941087|FD-Score:3.51|P-value:8.38E-4 ID:SGTC_2277|Compound:7947813|FD-Score:3.82|P-value:3.26E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:-3.70|P-value:7.24E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:4.14|P-value:1.11E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.61|P-value:9.41E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:-4.27|P-value:1.12E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:-3.95|P-value:3.34E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:-4.01|P-value:2.76E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:6.32|P-value:1.06E-8 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.31|P-value:1.19E-6 ID:SGTC_2739|Compound:tacrine|FD-Score:-6.30|P-value:2.29E-8 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-4.15|P-value:1.71E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-6.90|P-value:1.04E-9 ID:SGTC_2776|Compound:terbinafine|FD-Score:-3.66|P-value:8.22E-4 ID:SGTC_2827|Compound:7993847|FD-Score:4.03|P-value:1.64E-4 ID:SGTC_2838|Compound:9002687|FD-Score:-5.10|P-value:5.00E-6 ID:SGTC_2891|Compound:9063876|FD-Score:3.53|P-value:8.01E-4 ID:SGTC_2980|Compound:9014342|FD-Score:-5.51|P-value:8.83E-7 ID:SGTC_3148|Compound:9097893|FD-Score:3.78|P-value:3.68E-4 ID:SGTC_3209|Compound:9113616|FD-Score:-4.92|P-value:1.04E-5 ID:SGTC_3225|Compound:9130819|FD-Score:3.83|P-value:3.17E-4 ID:SGTC_3232|Compound:9132874|FD-Score:5.15|P-value:2.30E-6 ID:SGTC_70|Compound:0141-0289|FD-Score:-5.50|P-value:9.40E-7 ID:SGTC_87|Compound:0100-0052|FD-Score:4.07|P-value:1.40E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-8.29|P-value:2.72E-13 ID:SGTC_119|Compound:0121-0043|FD-Score:-5.94|P-value:1.29E-7 ID:SGTC_124|Compound:0136-0217|FD-Score:-5.10|P-value:5.08E-6 ID:SGTC_132|Compound:0417-1715|FD-Score:4.42|P-value:4.17E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:5.80|P-value:1.34E-7 ID:SGTC_163|Compound:1327-0036|FD-Score:6.04|P-value:4.25E-8 ID:SGTC_186|Compound:3455-3465|FD-Score:-4.20|P-value:1.45E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.55|P-value:7.47E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:6.43|P-value:6.10E-9 ID:SGTC_330|Compound:1488-0319|FD-Score:5.48|P-value:5.62E-7 ID:SGTC_350|Compound:k064-0020|FD-Score:-5.47|P-value:1.07E-6 ID:SGTC_393|Compound:acivicin|FD-Score:-4.82|P-value:1.52E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:3.76|P-value:3.89E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:-4.43|P-value:6.44E-5 ID:SGTC_621|Compound:3584-0052|FD-Score:-4.89|P-value:1.15E-5 ID:SGTC_667|Compound:1498-1044|FD-Score:3.57|P-value:6.97E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:-3.95|P-value:3.35E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.14|P-value:1.09E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.79|P-value:3.55E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:3.47|P-value:9.41E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.45|P-value:3.71E-5 ID:SGTC_828|Compound:2074-0507|FD-Score:4.74|P-value:1.23E-5 ID:SGTC_855|Compound:0428-0020|FD-Score:3.61|P-value:6.34E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:4.49|P-value:3.16E-5 ID:SGTC_894|Compound:0960-0238|FD-Score:5.53|P-value:4.52E-7 ID:SGTC_908|Compound:3448-7491|FD-Score:-3.90|P-value:3.86E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-3.76|P-value:5.90E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.53|P-value:8.04E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:-4.25|P-value:1.21E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:6.16|P-value:2.28E-8 ID:SGTC_1250|Compound:0485-0349|FD-Score:3.83|P-value:3.13E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:3.72|P-value:4.39E-4 ID:SGTC_1466|Compound:k213-0079|FD-Score:5.24|P-value:1.58E-6 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.97|P-value:1.99E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.20|P-value:8.96E-5 ID:SGTC_1766|Compound:st045414|FD-Score:8.77|P-value:4.47E-15 ID:SGTC_1772|Compound:st048439|FD-Score:3.77|P-value:3.85E-4 ID:SGTC_1797|Compound:st048695|FD-Score:-4.64|P-value:2.98E-5 ID:SGTC_1834|Compound:st055981|FD-Score:3.47|P-value:9.49E-4 ID:SGTC_1886|Compound:st057356|FD-Score:-3.90|P-value:3.81E-4 ID:SGTC_2013|Compound:4024628|FD-Score:-3.86|P-value:4.34E-4 ID:SGTC_2022|Compound:5136654|FD-Score:3.59|P-value:6.67E-4 ID:SGTC_2057|Compound:5236571|FD-Score:4.12|P-value:1.17E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.48|P-value:9.17E-4 ID:SGTC_2137|Compound:5261020|FD-Score:-3.93|P-value:3.52E-4 ID:SGTC_2156|Compound:5649887|FD-Score:5.13|P-value:2.52E-6 ID:SGTC_2248|Compound:tofa|FD-Score:-4.63|P-value:3.08E-5 ID:SGTC_2255|Compound:7941087|FD-Score:3.51|P-value:8.38E-4 ID:SGTC_2277|Compound:7947813|FD-Score:3.82|P-value:3.26E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:-3.70|P-value:7.24E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:4.14|P-value:1.11E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.61|P-value:9.41E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:-4.27|P-value:1.12E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:-3.95|P-value:3.34E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:-4.01|P-value:2.76E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:6.32|P-value:1.06E-8 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.31|P-value:1.19E-6 ID:SGTC_2739|Compound:tacrine|FD-Score:-6.30|P-value:2.29E-8 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-4.15|P-value:1.71E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-6.90|P-value:1.04E-9 ID:SGTC_2776|Compound:terbinafine|FD-Score:-3.66|P-value:8.22E-4 ID:SGTC_2827|Compound:7993847|FD-Score:4.03|P-value:1.64E-4 ID:SGTC_2838|Compound:9002687|FD-Score:-5.10|P-value:5.00E-6 ID:SGTC_2891|Compound:9063876|FD-Score:3.53|P-value:8.01E-4 ID:SGTC_2980|Compound:9014342|FD-Score:-5.51|P-value:8.83E-7 ID:SGTC_3148|Compound:9097893|FD-Score:3.78|P-value:3.68E-4 ID:SGTC_3209|Compound:9113616|FD-Score:-4.92|P-value:1.04E-5 ID:SGTC_3225|Compound:9130819|FD-Score:3.83|P-value:3.17E-4 ID:SGTC_3232|Compound:9132874|FD-Score:5.15|P-value:2.30E-6

Top fitness defect scores for YER025W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1766 st045414 8.77 44.8 µM 4.47E-15 RPP1 & pyrimidine depletion 3 8.96E-19 1.51
2 SGTC_274 sirtinol 6.43 40.1 µM 6.10E-9 6 6.57E-11 0.23
3 SGTC_2651 1327-0036 6.32 5.0 µM 1.06E-8 29 1.34E-10 0.03
4 SGTC_1213 1315-0166 6.16 115.0 µM 2.28E-8 4 3.55E-10 0.68
5 SGTC_163 1327-0036 6.04 7.5 µM 4.25E-8 33 7.85E-10 0.24
6 SGTC_134 0269-0018 5.80 6.9 µM 1.34E-7 9 3.41E-9 0.45
7 SGTC_894 0960-0238 5.53 55.9 µM 4.52E-7 5 1.60E-8 0.80
8 SGTC_330 1488-0319 5.48 80.1 µM 5.62E-7 RPP1 & pyrimidine depletion 3 2.11E-8 0.04
9 SGTC_2679 rapamycin 5.31 1.0 nM 1.19E-6 30 5.49E-8 0.05
10 SGTC_1466 k213-0079 5.24 57.5 µM 1.58E-6 1 7.88E-8 0.46
11 SGTC_3232 9132874 5.15 49.5 µM 2.30E-6 RPP1 & pyrimidine depletion 3 1.27E-7 0.30
12 SGTC_2156 5649887 5.13 115.0 µM 2.52E-6 RPP1 & pyrimidine depletion 8 1.42E-7 0.17
13 SGTC_828 2074-0507 4.74 139.0 µM 1.23E-5 RPP1 & pyrimidine depletion 5 1.07E-6 0.36
14 SGTC_878 0828-0289 4.49 13.7 µM 3.16E-5 17 3.54E-6 0.20
15 SGTC_800 3448-7362 4.45 155.0 µM 3.71E-5 translation 41 4.32E-6 0.16
16 SGTC_132 0417-1715 4.42 25.8 µM 4.17E-5 RPP1 & pyrimidine depletion 8 5.02E-6 0.20
17 SGTC_1754 st042065 4.20 16.1 µM 8.96E-5 NEO1-PIK1 28 1.32E-5 0.04
18 SGTC_682 0kpi-0099 4.14 17.0 µM 1.09E-4 26 1.70E-5 0.09
19 SGTC_2565 isoliquiritigenin 4.14 100.0 µM 1.11E-4 9 1.74E-5 0.12
20 SGTC_2057 5236571 4.12 38.3 µM 1.17E-4 RPP1 & pyrimidine depletion 13 1.85E-5 0.13

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.390 4.76E-122 YPL237W SUI3 het Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
0.328 1.20E-84 YPL238C_d YPL238C_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
0.228 7.87E-41 YJR007W SUI2 het Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
0.184 4.96E-27 YDL150W RPC53 het RNA polymerase III subunit C53
0.184 5.53E-27 YDR429C TIF35 het eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
0.157 6.75E-20 YOR146W_d YOR146W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
0.155 1.78E-19 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.155 2.10E-19 YNL236W SIN4 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.152 8.41E-19 YCR053W THR4 hom Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
0.147 1.28E-17 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.146 1.88E-17 YGR083C GCD2 het Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.145 3.49E-17 YLR044C PDC1 hom Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism
0.144 5.97E-17 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.141 2.88E-16 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.136 3.05E-15 YGL116W CDC20 het Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress