YER032W / FIR1

Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YER032W / FIR1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:5.22|P-value:2.68E-7 ID:SGTC_79|Compound:0052-0007|FD-Score:-3.30|P-value:5.57E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:3.32|P-value:7.81E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.45|P-value:4.88E-4 ID:SGTC_512|Compound:prima-1|FD-Score:5.06|P-value:6.06E-7 ID:SGTC_694|Compound:1315-0438|FD-Score:4.11|P-value:4.18E-5 ID:SGTC_707|Compound:0865-0008|FD-Score:-4.21|P-value:1.70E-5 ID:SGTC_825|Compound:1988-2224|FD-Score:3.77|P-value:1.56E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:-4.63|P-value:2.65E-6 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.53|P-value:3.80E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.88|P-value:1.04E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:-3.15|P-value:9.10E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:-3.37|P-value:4.26E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.74|P-value:1.77E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.27|P-value:9.21E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.73|P-value:1.79E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.57|P-value:3.19E-4 ID:SGTC_1625|Compound:st003711|FD-Score:4.27|P-value:2.20E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.95|P-value:7.93E-5 ID:SGTC_1847|Compound:st056231|FD-Score:5.64|P-value:2.97E-8 ID:SGTC_2065|Compound:5221596|FD-Score:-3.54|P-value:2.36E-4 ID:SGTC_2068|Compound:5226020|FD-Score:4.50|P-value:8.11E-6 ID:SGTC_2128|Compound:5255449|FD-Score:6.03|P-value:3.23E-9 ID:SGTC_2284|Compound:7945075|FD-Score:-3.41|P-value:3.69E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-3.57|P-value:2.11E-4 ID:SGTC_2424|Compound:5537928|FD-Score:4.46|P-value:9.38E-6 ID:SGTC_2483|Compound:5729050|FD-Score:-3.99|P-value:4.13E-5 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.68|P-value:2.17E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.55|P-value:3.42E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.28|P-value:5.79E-4 ID:SGTC_2783|Compound:7738165|FD-Score:3.59|P-value:3.00E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.36|P-value:6.77E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.38|P-value:4.19E-4 ID:SGTC_2878|Compound:9043973|FD-Score:-3.68|P-value:1.40E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.15|P-value:3.50E-5 ID:SGTC_2911|Compound:7942208|FD-Score:-3.20|P-value:7.66E-4 ID:SGTC_3153|Compound:9100258|FD-Score:3.86|P-value:1.10E-4 ID:SGTC_3155|Compound:9099265|FD-Score:-4.02|P-value:3.76E-5 ID:SGTC_3159|Compound:9100096|FD-Score:6.73|P-value:4.45E-11 ID:SGTC_3179|Compound:9107801|FD-Score:6.88|P-value:1.71E-11 ID:SGTC_58|Compound:0957-0393|FD-Score:5.22|P-value:2.68E-7 ID:SGTC_79|Compound:0052-0007|FD-Score:-3.30|P-value:5.57E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:3.32|P-value:7.81E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.45|P-value:4.88E-4 ID:SGTC_512|Compound:prima-1|FD-Score:5.06|P-value:6.06E-7 ID:SGTC_694|Compound:1315-0438|FD-Score:4.11|P-value:4.18E-5 ID:SGTC_707|Compound:0865-0008|FD-Score:-4.21|P-value:1.70E-5 ID:SGTC_825|Compound:1988-2224|FD-Score:3.77|P-value:1.56E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:-4.63|P-value:2.65E-6 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.53|P-value:3.80E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.88|P-value:1.04E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:-3.15|P-value:9.10E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:-3.37|P-value:4.26E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.74|P-value:1.77E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.27|P-value:9.21E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:3.73|P-value:1.79E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.57|P-value:3.19E-4 ID:SGTC_1625|Compound:st003711|FD-Score:4.27|P-value:2.20E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.95|P-value:7.93E-5 ID:SGTC_1847|Compound:st056231|FD-Score:5.64|P-value:2.97E-8 ID:SGTC_2065|Compound:5221596|FD-Score:-3.54|P-value:2.36E-4 ID:SGTC_2068|Compound:5226020|FD-Score:4.50|P-value:8.11E-6 ID:SGTC_2128|Compound:5255449|FD-Score:6.03|P-value:3.23E-9 ID:SGTC_2284|Compound:7945075|FD-Score:-3.41|P-value:3.69E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-3.57|P-value:2.11E-4 ID:SGTC_2424|Compound:5537928|FD-Score:4.46|P-value:9.38E-6 ID:SGTC_2483|Compound:5729050|FD-Score:-3.99|P-value:4.13E-5 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.68|P-value:2.17E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.55|P-value:3.42E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.28|P-value:5.79E-4 ID:SGTC_2783|Compound:7738165|FD-Score:3.59|P-value:3.00E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.36|P-value:6.77E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.38|P-value:4.19E-4 ID:SGTC_2878|Compound:9043973|FD-Score:-3.68|P-value:1.40E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.15|P-value:3.50E-5 ID:SGTC_2911|Compound:7942208|FD-Score:-3.20|P-value:7.66E-4 ID:SGTC_3153|Compound:9100258|FD-Score:3.86|P-value:1.10E-4 ID:SGTC_3155|Compound:9099265|FD-Score:-4.02|P-value:3.76E-5 ID:SGTC_3159|Compound:9100096|FD-Score:6.73|P-value:4.45E-11 ID:SGTC_3179|Compound:9107801|FD-Score:6.88|P-value:1.71E-11

Top fitness defect scores for YER032W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3179 9107801 6.88 49.5 µM 1.71E-11 1 2.97E-12
2 SGTC_3159 9100096 6.73 49.5 µM 4.45E-11 2 8.23E-12
3 SGTC_2128 5255449 6.03 168.5 µM 3.23E-9 4 7.99E-10
4 SGTC_1847 st056231 5.64 15.0 µM 2.97E-8 8 8.56E-9
5 SGTC_58 0957-0393 5.22 9.0 µM 2.68E-7 cell wall 261 8.96E-8
6 SGTC_512 prima-1 5.06 270.0 µM 6.06E-7 DNA intercalators 9 2.15E-7
7 SGTC_2068 5226020 4.50 147.0 µM 8.11E-6 19 3.44E-6
8 SGTC_2424 5537928 4.46 59.2 µM 9.38E-6 25 4.02E-6
9 SGTC_1625 st003711 4.27 30.1 µM 2.20E-5 calcium & mitochondrial duress 29 9.99E-6
10 SGTC_2896 9052656 4.15 71.4 µM 3.50E-5 endomembrane recycling 99 1.64E-5
11 SGTC_694 1315-0438 4.11 43.2 µM 4.18E-5 9 1.99E-5
12 SGTC_1789 tunicamycin 3.95 25.0 nM 7.93E-5 unfolded protein response 49 3.95E-5
13 SGTC_1091 telmisartan 3.88 47.6 µM 1.04E-4 15 5.28E-5
14 SGTC_3153 9100258 3.86 49.5 µM 1.10E-4 21 5.60E-5
15 SGTC_825 1988-2224 3.77 89.6 µM 1.56E-4 cell wall signaling 38 8.12E-5
16 SGTC_1341 1486-1360 3.74 280.0 µM 1.77E-4 32 9.33E-5
17 SGTC_1493 4348-0038 3.73 111.0 µM 1.79E-4 RSC complex & mRNA processing 39 9.46E-5
18 SGTC_2536 dehydroabietamide 3.68 32.0 µM 2.17E-4 45 1.16E-4
19 SGTC_2783 7738165 3.59 71.4 µM 3.00E-4 43 1.64E-4
20 SGTC_1584 6-fluoro-dl-tryptophan 3.57 90.0 µM 3.19E-4 mitochondrial processes 25 1.76E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.157 6.43E-20 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.128 8.49E-14 YBR292C_d YBR292C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene
0.121 2.22E-12 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.118 5.99E-12 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.118 6.92E-12 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.114 3.44E-11 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.114 3.44E-11 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.113 4.39E-11 YHL009C YAP3 hom Basic leucine zipper (bZIP) transcription factor
0.110 1.45E-10 YJL192C SOP4 hom ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER
0.110 1.78E-10 YML057W CMP2 hom Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
0.109 2.10E-10 YGR287C IMA1 hom Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family
0.108 3.53E-10 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.107 5.24E-10 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.106 6.64E-10 YOL102C TPT1 het tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
0.104 1.79E-9 YDR001C NTH1 hom Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication