YER033C / ZRG8

Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency

Zygosity: Homozygous strain
fixedexpanded
Profile for YER033C / ZRG8

Click on Significant Values for Screen Details ID:SGTC_141|Compound:0443-0274|FD-Score:-3.72|P-value:1.21E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:3.99|P-value:4.33E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.32|P-value:5.48E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:3.32|P-value:5.35E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:3.42|P-value:3.78E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:3.48|P-value:3.11E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:3.22|P-value:7.55E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:-3.26|P-value:6.48E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:-4.72|P-value:1.65E-6 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:3.45|P-value:3.48E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.56|P-value:2.30E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-4.82|P-value:1.00E-6 ID:SGTC_1148|Compound:3909-7663|FD-Score:3.43|P-value:3.65E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.19|P-value:8.42E-4 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.10|P-value:2.78E-5 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.84|P-value:7.88E-5 ID:SGTC_1240|Compound:0356-0016|FD-Score:3.90|P-value:6.08E-5 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.89|P-value:6.18E-5 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.84|P-value:7.82E-5 ID:SGTC_1389|Compound:3405-0187|FD-Score:-3.52|P-value:2.61E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.48|P-value:3.11E-4 ID:SGTC_1652|Compound:st011932|FD-Score:3.39|P-value:4.20E-4 ID:SGTC_1674|Compound:st016616|FD-Score:6.33|P-value:2.31E-10 ID:SGTC_1678|Compound:st019265|FD-Score:3.70|P-value:1.36E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.27|P-value:6.38E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.37|P-value:4.53E-4 ID:SGTC_1791|Compound:st048313|FD-Score:3.50|P-value:2.84E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.78|P-value:9.85E-5 ID:SGTC_1902|Compound:st060212|FD-Score:-3.81|P-value:8.60E-5 ID:SGTC_1904|Compound:st060225|FD-Score:-3.36|P-value:4.67E-4 ID:SGTC_1940|Compound:st075990|FD-Score:4.15|P-value:2.22E-5 ID:SGTC_1948|Compound:st076329|FD-Score:3.19|P-value:8.37E-4 ID:SGTC_2110|Compound:5483698|FD-Score:-3.44|P-value:3.54E-4 ID:SGTC_2240|Compound:6690706|FD-Score:-3.20|P-value:8.10E-4 ID:SGTC_2334|Compound:9017922|FD-Score:-3.30|P-value:5.67E-4 ID:SGTC_2435|Compound:5706476|FD-Score:3.88|P-value:6.60E-5 ID:SGTC_2513|Compound:baicalein|FD-Score:-3.63|P-value:1.71E-4 ID:SGTC_2517|Compound:totarol|FD-Score:-6.39|P-value:1.56E-10 ID:SGTC_2707|Compound:st078556|FD-Score:5.23|P-value:1.32E-7 ID:SGTC_2715|Compound:st079500|FD-Score:-3.93|P-value:5.32E-5 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.56|P-value:2.27E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:3.21|P-value:7.94E-4 ID:SGTC_2790|Compound:5423970|FD-Score:3.27|P-value:6.46E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.45|P-value:3.47E-4 ID:SGTC_2828|Compound:7998861|FD-Score:3.60|P-value:1.96E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-3.58|P-value:2.10E-4 ID:SGTC_2840|Compound:9008771|FD-Score:3.55|P-value:2.41E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.58|P-value:2.10E-4 ID:SGTC_3085|Compound:9117376|FD-Score:3.83|P-value:8.06E-5 ID:SGTC_3166|Compound:9102297|FD-Score:-3.20|P-value:8.00E-4 ID:SGTC_3170|Compound:9103054|FD-Score:3.47|P-value:3.21E-4 ID:SGTC_3185|Compound:9108267|FD-Score:3.18|P-value:8.83E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.18|P-value:8.67E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.70|P-value:1.33E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.72|P-value:1.21E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:3.99|P-value:4.33E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.32|P-value:5.48E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:3.32|P-value:5.35E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:3.42|P-value:3.78E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:3.48|P-value:3.11E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:3.22|P-value:7.55E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:-3.26|P-value:6.48E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:-4.72|P-value:1.65E-6 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:3.45|P-value:3.48E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.56|P-value:2.30E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-4.82|P-value:1.00E-6 ID:SGTC_1148|Compound:3909-7663|FD-Score:3.43|P-value:3.65E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:3.19|P-value:8.42E-4 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.10|P-value:2.78E-5 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.84|P-value:7.88E-5 ID:SGTC_1240|Compound:0356-0016|FD-Score:3.90|P-value:6.08E-5 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.89|P-value:6.18E-5 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.84|P-value:7.82E-5 ID:SGTC_1389|Compound:3405-0187|FD-Score:-3.52|P-value:2.61E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.48|P-value:3.11E-4 ID:SGTC_1652|Compound:st011932|FD-Score:3.39|P-value:4.20E-4 ID:SGTC_1674|Compound:st016616|FD-Score:6.33|P-value:2.31E-10 ID:SGTC_1678|Compound:st019265|FD-Score:3.70|P-value:1.36E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.27|P-value:6.38E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.37|P-value:4.53E-4 ID:SGTC_1791|Compound:st048313|FD-Score:3.50|P-value:2.84E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.78|P-value:9.85E-5 ID:SGTC_1902|Compound:st060212|FD-Score:-3.81|P-value:8.60E-5 ID:SGTC_1904|Compound:st060225|FD-Score:-3.36|P-value:4.67E-4 ID:SGTC_1940|Compound:st075990|FD-Score:4.15|P-value:2.22E-5 ID:SGTC_1948|Compound:st076329|FD-Score:3.19|P-value:8.37E-4 ID:SGTC_2110|Compound:5483698|FD-Score:-3.44|P-value:3.54E-4 ID:SGTC_2240|Compound:6690706|FD-Score:-3.20|P-value:8.10E-4 ID:SGTC_2334|Compound:9017922|FD-Score:-3.30|P-value:5.67E-4 ID:SGTC_2435|Compound:5706476|FD-Score:3.88|P-value:6.60E-5 ID:SGTC_2513|Compound:baicalein|FD-Score:-3.63|P-value:1.71E-4 ID:SGTC_2517|Compound:totarol|FD-Score:-6.39|P-value:1.56E-10 ID:SGTC_2707|Compound:st078556|FD-Score:5.23|P-value:1.32E-7 ID:SGTC_2715|Compound:st079500|FD-Score:-3.93|P-value:5.32E-5 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.56|P-value:2.27E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:3.21|P-value:7.94E-4 ID:SGTC_2790|Compound:5423970|FD-Score:3.27|P-value:6.46E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.45|P-value:3.47E-4 ID:SGTC_2828|Compound:7998861|FD-Score:3.60|P-value:1.96E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-3.58|P-value:2.10E-4 ID:SGTC_2840|Compound:9008771|FD-Score:3.55|P-value:2.41E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.58|P-value:2.10E-4 ID:SGTC_3085|Compound:9117376|FD-Score:3.83|P-value:8.06E-5 ID:SGTC_3166|Compound:9102297|FD-Score:-3.20|P-value:8.00E-4 ID:SGTC_3170|Compound:9103054|FD-Score:3.47|P-value:3.21E-4 ID:SGTC_3185|Compound:9108267|FD-Score:3.18|P-value:8.83E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.18|P-value:8.67E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.70|P-value:1.33E-4

Top fitness defect scores for YER033C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1674 st016616 6.33 23.6 µM 2.31E-10 2 1.23E-10
2 SGTC_2707 st078556 5.23 66.6 µM 1.32E-7 4 8.53E-8
3 SGTC_1940 st075990 4.15 51.1 µM 2.22E-5 60S ribosome export 17 1.67E-5
4 SGTC_1174 0739-0097 4.10 51.1 µM 2.78E-5 redox potentiating 27 2.11E-5
5 SGTC_146 3448-8650 3.99 53.1 µM 4.33E-5 NEO1-PIK1 134 3.33E-5
6 SGTC_1240 0356-0016 3.90 42.1 µM 6.08E-5 mitochondrial response to ROS 36 4.72E-5
7 SGTC_2435 5706476 3.88 10.6 µM 6.60E-5 27 5.13E-5
8 SGTC_1340 1486-1359 3.84 257.0 µM 7.82E-5 23 6.11E-5
9 SGTC_1203 3992-3522 3.84 103.0 µM 7.88E-5 40 6.16E-5
10 SGTC_3085 9117376 3.83 49.5 µM 8.06E-5 11 6.30E-5
11 SGTC_1892 st058411 3.78 7.4 µM 9.85E-5 27 7.75E-5
12 SGTC_3316 9136639 3.70 10.6 µM 1.33E-4 17 1.06E-4
13 SGTC_1678 st019265 3.70 80.2 µM 1.36E-4 60S ribosome export 28 1.08E-4
14 SGTC_2828 7998861 3.60 71.4 µM 1.96E-4 22 1.58E-4
15 SGTC_3061 glucosamine 3.58 200.0 µM 2.10E-4 17 1.69E-4
16 SGTC_2764 methyldopa 3.56 94.7 µM 2.27E-4 25 1.83E-4
17 SGTC_1122 capsaicin 3.56 818.6 nM 2.30E-4 39 1.86E-4
18 SGTC_2840 9008771 3.55 71.4 µM 2.41E-4 13 1.94E-4
19 SGTC_1791 st048313 3.50 56.1 µM 2.84E-4 29 2.30E-4
20 SGTC_1609 st001574 3.48 46.4 µM 3.11E-4 25 2.53E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.163 2.19E-21 YDR243C PRP28 het RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
0.091 1.38E-7 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.090 1.93E-7 YDR308C SRB7 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
0.083 1.45E-6 YFR031C SMC2 het Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
0.083 1.64E-6 YNL254C RTC4 hom Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.079 4.24E-6 YDR419W RAD30 hom DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV
0.078 5.54E-6 YNL311C SKP2 hom F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments
0.076 1.15E-5 YDR440W DOT1 hom Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response
0.076 1.18E-5 YDL214C PRR2 hom Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance
0.075 1.21E-5 YER046W SPO73 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis
0.074 1.93E-5 YPL155C KIP2 hom Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle
0.073 2.31E-5 YJL074C SMC3 het Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
0.072 3.23E-5 YOR370C MRS6 het Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress
0.072 3.25E-5 YMR048W CSM3 hom Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.070 4.64E-5 YKL086W SRX1 hom Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress