YER048W-A / ISD11

Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER048W-A / ISD11

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.22|P-value:2.67E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:14.80|P-value:6.38E-57 ID:SGTC_42|Compound:0133-0086|FD-Score:7.04|P-value:2.28E-14 ID:SGTC_57|Compound:miconazole|FD-Score:4.73|P-value:1.96E-7 ID:SGTC_137|Compound:0438-0332|FD-Score:5.16|P-value:1.60E-8 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.49|P-value:2.76E-9 ID:SGTC_171|Compound:k064-0041|FD-Score:3.95|P-value:1.12E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:2.92|P-value:8.32E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:6.07|P-value:3.95E-11 ID:SGTC_350|Compound:k064-0020|FD-Score:2.90|P-value:9.13E-4 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:2.92|P-value:8.34E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.49|P-value:1.11E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.02|P-value:7.91E-6 ID:SGTC_576|Compound:1073-0113|FD-Score:4.24|P-value:2.69E-6 ID:SGTC_584|Compound:0986-0246|FD-Score:4.97|P-value:4.88E-8 ID:SGTC_607|Compound:4491-0986|FD-Score:3.09|P-value:4.35E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.09|P-value:5.69E-6 ID:SGTC_769|Compound:0302-0231|FD-Score:3.65|P-value:4.45E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:3.29|P-value:2.04E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:-3.25|P-value:2.97E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.92|P-value:1.28E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:-3.51|P-value:9.98E-5 ID:SGTC_1697|Compound:st025554|FD-Score:-3.21|P-value:3.43E-4 ID:SGTC_1706|Compound:st031321|FD-Score:3.02|P-value:5.79E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.84|P-value:1.86E-5 ID:SGTC_1746|Compound:st038117|FD-Score:3.72|P-value:3.23E-5 ID:SGTC_1761|Compound:st044296|FD-Score:-6.90|P-value:1.11E-13 ID:SGTC_1766|Compound:st045414|FD-Score:6.30|P-value:7.34E-12 ID:SGTC_1767|Compound:st044992|FD-Score:-3.23|P-value:3.21E-4 ID:SGTC_1898|Compound:st060199|FD-Score:-2.93|P-value:9.65E-4 ID:SGTC_1902|Compound:st060212|FD-Score:-2.93|P-value:9.88E-4 ID:SGTC_1987|Compound:st072290|FD-Score:2.98|P-value:6.67E-4 ID:SGTC_2013|Compound:4024628|FD-Score:3.30|P-value:1.97E-4 ID:SGTC_2015|Compound:5119600|FD-Score:3.44|P-value:1.07E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.46|P-value:9.89E-5 ID:SGTC_2181|Compound:6316240|FD-Score:2.99|P-value:6.34E-4 ID:SGTC_2183|Compound:6420625|FD-Score:-4.23|P-value:3.67E-6 ID:SGTC_2239|Compound:6685329|FD-Score:-5.34|P-value:7.02E-9 ID:SGTC_2240|Compound:6690706|FD-Score:-5.43|P-value:4.19E-9 ID:SGTC_2246|Compound:7293507|FD-Score:3.26|P-value:2.25E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.53|P-value:7.38E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:2.88|P-value:9.49E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.22|P-value:2.64E-4 ID:SGTC_2800|Compound:7887935|FD-Score:2.92|P-value:8.44E-4 ID:SGTC_2856|Compound:9022925|FD-Score:-3.22|P-value:3.33E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-2.98|P-value:7.99E-4 ID:SGTC_2924|Compound:9010168|FD-Score:4.28|P-value:2.20E-6 ID:SGTC_2926|Compound:9010726|FD-Score:-5.95|P-value:1.34E-10 ID:SGTC_2998|Compound:9066762|FD-Score:4.27|P-value:2.32E-6 ID:SGTC_3079|Compound:9117512|FD-Score:3.72|P-value:3.21E-5 ID:SGTC_3114|Compound:9122766|FD-Score:-3.70|P-value:4.40E-5 ID:SGTC_3130|Compound:9127414|FD-Score:3.00|P-value:6.23E-4 ID:SGTC_3133|Compound:9127736|FD-Score:-3.09|P-value:5.42E-4 ID:SGTC_3157|Compound:9099274|FD-Score:-3.30|P-value:2.38E-4 ID:SGTC_3189|Compound:9110682|FD-Score:4.52|P-value:5.99E-7 ID:SGTC_3196|Compound:9111891|FD-Score:3.17|P-value:3.18E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-3.42|P-value:1.48E-4 ID:SGTC_3292|Compound:9120825|FD-Score:-3.35|P-value:1.98E-4 ID:SGTC_3342|Compound:9147791|FD-Score:2.98|P-value:6.66E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.64|P-value:4.61E-5 ID:SGTC_3353|Compound:9151644|FD-Score:3.37|P-value:1.45E-4 ID:SGTC_3|Compound:0833-0587|FD-Score:3.22|P-value:2.67E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:14.80|P-value:6.38E-57 ID:SGTC_42|Compound:0133-0086|FD-Score:7.04|P-value:2.28E-14 ID:SGTC_57|Compound:miconazole|FD-Score:4.73|P-value:1.96E-7 ID:SGTC_137|Compound:0438-0332|FD-Score:5.16|P-value:1.60E-8 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.49|P-value:2.76E-9 ID:SGTC_171|Compound:k064-0041|FD-Score:3.95|P-value:1.12E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:2.92|P-value:8.32E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:6.07|P-value:3.95E-11 ID:SGTC_350|Compound:k064-0020|FD-Score:2.90|P-value:9.13E-4 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:2.92|P-value:8.34E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.49|P-value:1.11E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.02|P-value:7.91E-6 ID:SGTC_576|Compound:1073-0113|FD-Score:4.24|P-value:2.69E-6 ID:SGTC_584|Compound:0986-0246|FD-Score:4.97|P-value:4.88E-8 ID:SGTC_607|Compound:4491-0986|FD-Score:3.09|P-value:4.35E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.09|P-value:5.69E-6 ID:SGTC_769|Compound:0302-0231|FD-Score:3.65|P-value:4.45E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:3.29|P-value:2.04E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:-3.25|P-value:2.97E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.92|P-value:1.28E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:-3.51|P-value:9.98E-5 ID:SGTC_1697|Compound:st025554|FD-Score:-3.21|P-value:3.43E-4 ID:SGTC_1706|Compound:st031321|FD-Score:3.02|P-value:5.79E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.84|P-value:1.86E-5 ID:SGTC_1746|Compound:st038117|FD-Score:3.72|P-value:3.23E-5 ID:SGTC_1761|Compound:st044296|FD-Score:-6.90|P-value:1.11E-13 ID:SGTC_1766|Compound:st045414|FD-Score:6.30|P-value:7.34E-12 ID:SGTC_1767|Compound:st044992|FD-Score:-3.23|P-value:3.21E-4 ID:SGTC_1898|Compound:st060199|FD-Score:-2.93|P-value:9.65E-4 ID:SGTC_1902|Compound:st060212|FD-Score:-2.93|P-value:9.88E-4 ID:SGTC_1987|Compound:st072290|FD-Score:2.98|P-value:6.67E-4 ID:SGTC_2013|Compound:4024628|FD-Score:3.30|P-value:1.97E-4 ID:SGTC_2015|Compound:5119600|FD-Score:3.44|P-value:1.07E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.46|P-value:9.89E-5 ID:SGTC_2181|Compound:6316240|FD-Score:2.99|P-value:6.34E-4 ID:SGTC_2183|Compound:6420625|FD-Score:-4.23|P-value:3.67E-6 ID:SGTC_2239|Compound:6685329|FD-Score:-5.34|P-value:7.02E-9 ID:SGTC_2240|Compound:6690706|FD-Score:-5.43|P-value:4.19E-9 ID:SGTC_2246|Compound:7293507|FD-Score:3.26|P-value:2.25E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.53|P-value:7.38E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:2.88|P-value:9.49E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.22|P-value:2.64E-4 ID:SGTC_2800|Compound:7887935|FD-Score:2.92|P-value:8.44E-4 ID:SGTC_2856|Compound:9022925|FD-Score:-3.22|P-value:3.33E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-2.98|P-value:7.99E-4 ID:SGTC_2924|Compound:9010168|FD-Score:4.28|P-value:2.20E-6 ID:SGTC_2926|Compound:9010726|FD-Score:-5.95|P-value:1.34E-10 ID:SGTC_2998|Compound:9066762|FD-Score:4.27|P-value:2.32E-6 ID:SGTC_3079|Compound:9117512|FD-Score:3.72|P-value:3.21E-5 ID:SGTC_3114|Compound:9122766|FD-Score:-3.70|P-value:4.40E-5 ID:SGTC_3130|Compound:9127414|FD-Score:3.00|P-value:6.23E-4 ID:SGTC_3133|Compound:9127736|FD-Score:-3.09|P-value:5.42E-4 ID:SGTC_3157|Compound:9099274|FD-Score:-3.30|P-value:2.38E-4 ID:SGTC_3189|Compound:9110682|FD-Score:4.52|P-value:5.99E-7 ID:SGTC_3196|Compound:9111891|FD-Score:3.17|P-value:3.18E-4 ID:SGTC_3291|Compound:9121177|FD-Score:-3.42|P-value:1.48E-4 ID:SGTC_3292|Compound:9120825|FD-Score:-3.35|P-value:1.98E-4 ID:SGTC_3342|Compound:9147791|FD-Score:2.98|P-value:6.66E-4 ID:SGTC_3347|Compound:9188721|FD-Score:3.64|P-value:4.61E-5 ID:SGTC_3353|Compound:9151644|FD-Score:3.37|P-value:1.45E-4

Top fitness defect scores for YER048W-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_36 0335-0881 14.80 14.2 µM 6.38E-57 ubiquinone biosynthesis & proteasome 2 4.43E-50 4.19
2 SGTC_42 0133-0086 7.04 60.6 µM 2.28E-14 1 9.34E-13 1.39
3 SGTC_1766 st045414 6.30 44.8 µM 7.34E-12 RPP1 & pyrimidine depletion 5 1.48E-10 0.25
4 SGTC_284 0988-0104 6.07 32.6 µM 3.95E-11 heme requiring 2 6.48E-10 2.50
5 SGTC_137 0438-0332 5.16 85.4 µM 1.60E-8 ubiquinone biosynthesis & proteasome 25 1.26E-7 0.00
6 SGTC_584 0986-0246 4.97 15.4 µM 4.88E-8 heme requiring 12 3.35E-7 0.17
7 SGTC_57 miconazole 4.73 162.5 nM 1.96E-7 ergosterol depletion effects on membrane 20 1.14E-6 0.20
8 SGTC_3189 9110682 4.52 49.5 µM 5.99E-7 5 3.03E-6 0.08
9 SGTC_2924 9010168 4.28 62.8 µM 2.20E-6 cell wall signaling 4 9.50E-6 0.08
10 SGTC_2998 9066762 4.27 71.4 µM 2.32E-6 RPP1 & pyrimidine depletion 9 9.95E-6 0.20
11 SGTC_576 1073-0113 4.24 22.7 µM 2.69E-6 redox potentiating 39 1.13E-5 0.10
12 SGTC_674 0498-0004 4.09 451.0 µM 5.69E-6 heme requiring 17 2.19E-5 0.01
13 SGTC_525 k781-2675 4.02 26.7 µM 7.91E-6 redox potentiating 42 2.92E-5 0.27
14 SGTC_171 k064-0041 3.95 9.7 µM 1.12E-5 heme requiring 16 3.96E-5 0.32
15 SGTC_1282 0886-0010 3.92 32.4 µM 1.28E-5 endomembrane recycling 14 4.45E-5 0.23
16 SGTC_1707 st032323 3.84 6.3 µM 1.86E-5 DNA damage response 31 6.17E-5 0.03
17 SGTC_3079 9117512 3.72 49.5 µM 3.21E-5 1 9.97E-5 0.29
18 SGTC_1746 st038117 3.72 42.0 µM 3.23E-5 7 1.00E-4 0.20
19 SGTC_769 0302-0231 3.65 76.0 µM 4.45E-5 15 1.33E-4 0.11
20 SGTC_3347 9188721 3.64 44.7 µM 4.61E-5 12 1.37E-4 0.09

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.220 7.22E-38 YJL072C PSF2 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.188 5.29E-28 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.180 6.75E-26 YOL096C COQ3 hom O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
0.175 1.63E-24 YML110C COQ5 hom 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
0.165 6.81E-22 YPL124W SPC29 het Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
0.164 9.63E-22 YDL190C UFD2 hom Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3
0.154 2.39E-19 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.152 7.87E-19 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.151 1.30E-18 YDR232W HEM1 het 5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
0.150 2.12E-18 YBR088C POL30 het Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
0.150 2.40E-18 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.148 5.91E-18 YCR033W SNT1 hom Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance
0.145 3.61E-17 YPL126W NAN1 het U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA
0.139 4.86E-16 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.139 5.19E-16 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress