HIPHOP chemogenomics database

YER048W-A / ISD11

Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis

Zygosity: Heterozygous strain

fixedexpanded
Profile for YER048W-A / ISD11

Click on Significant Values for Screen Details

Top fitness defect scores for YER048W-A deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_36	0335-0881	14.80	14.2 µM	6.38E-57	ubiquinone biosynthesis & proteasome	2	4.43E-50	4.19
2	SGTC_42	0133-0086	7.04	60.6 µM	2.28E-14		1	9.34E-13	1.39
3	SGTC_1766	st045414	6.30	44.8 µM	7.34E-12	RPP1 & pyrimidine depletion	5	1.48E-10	0.25
4	SGTC_284	0988-0104	6.07	32.6 µM	3.95E-11	heme requiring	2	6.48E-10	2.50
5	SGTC_137	0438-0332	5.16	85.4 µM	1.60E-8	ubiquinone biosynthesis & proteasome	25	1.26E-7	0.00
6	SGTC_584	0986-0246	4.97	15.4 µM	4.88E-8	heme requiring	12	3.35E-7	0.17
7	SGTC_57	miconazole	4.73	162.5 nM	1.96E-7	ergosterol depletion effects on membrane	20	1.14E-6	0.20
8	SGTC_3189	9110682	4.52	49.5 µM	5.99E-7		5	3.03E-6	0.08
9	SGTC_2924	9010168	4.28	62.8 µM	2.20E-6	cell wall signaling	4	9.50E-6	0.08
10	SGTC_2998	9066762	4.27	71.4 µM	2.32E-6	RPP1 & pyrimidine depletion	9	9.95E-6	0.20
11	SGTC_576	1073-0113	4.24	22.7 µM	2.69E-6	redox potentiating	39	1.13E-5	0.10
12	SGTC_674	0498-0004	4.09	451.0 µM	5.69E-6	heme requiring	17	2.19E-5	0.01
13	SGTC_525	k781-2675	4.02	26.7 µM	7.91E-6	redox potentiating	42	2.92E-5	0.27
14	SGTC_171	k064-0041	3.95	9.7 µM	1.12E-5	heme requiring	16	3.96E-5	0.32
15	SGTC_1282	0886-0010	3.92	32.4 µM	1.28E-5	endomembrane recycling	14	4.45E-5	0.23
16	SGTC_1707	st032323	3.84	6.3 µM	1.86E-5	DNA damage response	31	6.17E-5	0.03
17	SGTC_3079	9117512	3.72	49.5 µM	3.21E-5		1	9.97E-5	0.29
18	SGTC_1746	st038117	3.72	42.0 µM	3.23E-5		7	1.00E-4	0.20
19	SGTC_769	0302-0231	3.65	76.0 µM	4.45E-5		15	1.33E-4	0.11
20	SGTC_3347	9188721	3.64	44.7 µM	4.61E-5		12	1.37E-4	0.09

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.220	7.22E-38	YJL072C	PSF2	het	Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.188	5.29E-28	YNR042W_d	YNR042W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.180	6.75E-26	YOL096C	COQ3	hom	O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
0.175	1.63E-24	YML110C	COQ5	hom	2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
0.165	6.81E-22	YPL124W	SPC29	het	Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
0.164	9.63E-22	YDL190C	UFD2	hom	Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3
0.154	2.39E-19	YDR537C_d	YDR537C_d	hom	Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.152	7.87E-19	YGR255C	COQ6	hom	Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.151	1.30E-18	YDR232W	HEM1	het	5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
0.150	2.12E-18	YBR088C	POL30	het	Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
0.150	2.40E-18	YML034C-A_d	YML034C-A_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.148	5.91E-18	YCR033W	SNT1	hom	Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance
0.145	3.61E-17	YPL126W	NAN1	het	U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA
0.139	4.86E-16	YDR204W	COQ4	hom	Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.139	5.19E-16	YHR079C	IRE1	hom	Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress