YER065C / ICL1

Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose

Zygosity: Homozygous strain
fixedexpanded
Profile for YER065C / ICL1

Click on Significant Values for Screen Details ID:SGTC_328|Compound:k915-0120|FD-Score:3.13|P-value:4.01E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.21|P-value:2.90E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.87|P-value:1.87E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.71|P-value:8.08E-5 ID:SGTC_884|Compound:0851-0618|FD-Score:3.51|P-value:8.89E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.31|P-value:3.96E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.23|P-value:5.41E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.02|P-value:6.04E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:2.90|P-value:9.17E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:-3.29|P-value:4.21E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.36|P-value:3.30E-4 ID:SGTC_1618|Compound:st003135|FD-Score:4.16|P-value:4.84E-6 ID:SGTC_1620|Compound:st003704|FD-Score:4.65|P-value:3.91E-7 ID:SGTC_1633|Compound:st005664|FD-Score:-3.59|P-value:1.33E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.14|P-value:7.30E-4 ID:SGTC_1821|Compound:st053465|FD-Score:-3.91|P-value:3.46E-5 ID:SGTC_1865|Compound:st057639|FD-Score:-3.78|P-value:5.92E-5 ID:SGTC_1956|Compound:st077085|FD-Score:-3.06|P-value:9.70E-4 ID:SGTC_2244|Compound:6635614|FD-Score:-3.17|P-value:6.61E-4 ID:SGTC_2275|Compound:7947463|FD-Score:3.10|P-value:4.46E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-4.78|P-value:5.20E-7 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.17|P-value:4.61E-6 ID:SGTC_2439|Compound:5492030|FD-Score:2.88|P-value:9.94E-4 ID:SGTC_2465|Compound:5809242|FD-Score:-3.36|P-value:3.24E-4 ID:SGTC_2625|Compound:euphol|FD-Score:5.17|P-value:2.10E-8 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.86|P-value:1.90E-5 ID:SGTC_2628|Compound:lathosterol|FD-Score:4.20|P-value:3.90E-6 ID:SGTC_3005|Compound:9076496|FD-Score:-3.54|P-value:1.62E-4 ID:SGTC_3019|Compound:9081971|FD-Score:-3.56|P-value:1.52E-4 ID:SGTC_328|Compound:k915-0120|FD-Score:3.13|P-value:4.01E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.21|P-value:2.90E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.87|P-value:1.87E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.71|P-value:8.08E-5 ID:SGTC_884|Compound:0851-0618|FD-Score:3.51|P-value:8.89E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.31|P-value:3.96E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.23|P-value:5.41E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.02|P-value:6.04E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:2.90|P-value:9.17E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:-3.29|P-value:4.21E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.36|P-value:3.30E-4 ID:SGTC_1618|Compound:st003135|FD-Score:4.16|P-value:4.84E-6 ID:SGTC_1620|Compound:st003704|FD-Score:4.65|P-value:3.91E-7 ID:SGTC_1633|Compound:st005664|FD-Score:-3.59|P-value:1.33E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.14|P-value:7.30E-4 ID:SGTC_1821|Compound:st053465|FD-Score:-3.91|P-value:3.46E-5 ID:SGTC_1865|Compound:st057639|FD-Score:-3.78|P-value:5.92E-5 ID:SGTC_1956|Compound:st077085|FD-Score:-3.06|P-value:9.70E-4 ID:SGTC_2244|Compound:6635614|FD-Score:-3.17|P-value:6.61E-4 ID:SGTC_2275|Compound:7947463|FD-Score:3.10|P-value:4.46E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-4.78|P-value:5.20E-7 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.17|P-value:4.61E-6 ID:SGTC_2439|Compound:5492030|FD-Score:2.88|P-value:9.94E-4 ID:SGTC_2465|Compound:5809242|FD-Score:-3.36|P-value:3.24E-4 ID:SGTC_2625|Compound:euphol|FD-Score:5.17|P-value:2.10E-8 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.86|P-value:1.90E-5 ID:SGTC_2628|Compound:lathosterol|FD-Score:4.20|P-value:3.90E-6 ID:SGTC_3005|Compound:9076496|FD-Score:-3.54|P-value:1.62E-4 ID:SGTC_3019|Compound:9081971|FD-Score:-3.56|P-value:1.52E-4

Top fitness defect scores for YER065C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2625 euphol 5.17 100.0 µM 2.10E-8 11 1.17E-7
2 SGTC_1620 st003704 4.65 16.5 µM 3.91E-7 7 1.66E-6
3 SGTC_2628 lathosterol 4.20 100.0 µM 3.90E-6 24 1.34E-5
4 SGTC_2407 paclitaxel 4.17 400.0 µM 4.61E-6 30 1.55E-5
5 SGTC_1618 st003135 4.16 44.5 µM 4.84E-6 13 1.63E-5
6 SGTC_742 0kpi-0133 3.87 140.0 µM 1.87E-5 ERG2 27 5.52E-5
7 SGTC_2627 acetyl isogambogic acid 3.86 100.0 µM 1.90E-5 TSC3-RPN4 49 5.61E-5
8 SGTC_884 0851-0618 3.51 334.0 µM 8.89E-5 25 2.27E-4
9 SGTC_446 go6976 3.21 132.0 µM 2.90E-4 36 6.60E-4
10 SGTC_328 k915-0120 3.13 63.5 µM 4.01E-4 TSC3-RPN4 42 8.84E-4
11 SGTC_2275 7947463 3.10 200.0 µM 4.46E-4 iron homeostasis 65 9.74E-4
12 SGTC_1340 1486-1359 3.02 257.0 µM 6.04E-4 58 0.00128
13 SGTC_1430 4049-0248 2.90 69.1 µM 9.17E-4 75 0.00187
14 SGTC_2439 5492030 2.88 7.2 µM 9.94E-4 60 0.00201
15 SGTC_3336 9143619 2.84 71.4 µM 0.00114 71 0.00227
16 SGTC_1343 1487-0095 2.83 224.0 µM 0.00116 45 0.00232
17 SGTC_2263 7948595 2.79 193.3 µM 0.00135 47 0.00264
18 SGTC_1434 4073-0095 2.77 163.0 µM 0.00144 57 0.00280
19 SGTC_2278 7948257 2.76 134.3 µM 0.00150 66 0.00292
20 SGTC_3017 9080681 2.74 49.5 µM 0.00157 80 0.00304

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.223 4.08E-39 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.181 2.97E-26 YKL140W TGL1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.168 1.46E-22 YDR046C BAP3 hom Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication
0.167 2.62E-22 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.166 3.47E-22 YMR139W RIM11 hom Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress
0.165 6.75E-22 YDR242W AMD2 hom Putative amidase
0.165 7.21E-22 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.157 5.22E-20 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.156 9.19E-20 YLR267W_p BOP2_p hom Protein of unknown function
0.150 2.36E-18 YMR104C YPK2 hom Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication
0.148 6.24E-18 YJL083W TAX4 hom EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication
0.147 9.65E-18 YGR207C CIR1 hom Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
0.144 5.89E-17 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.143 7.62E-17 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.143 8.36E-17 YML053C_p YML053C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene