YER083C / GET2

Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division

Zygosity: Homozygous strain
fixedexpanded
Profile for YER083C / GET2

Click on Significant Values for Screen Details ID:SGTC_59|Compound:0330-0160|FD-Score:4.58|P-value:3.83E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.85|P-value:1.83E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:4.97|P-value:1.29E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:4.31|P-value:7.81E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:7.04|P-value:1.06E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:7.03|P-value:1.12E-7 ID:SGTC_163|Compound:1327-0036|FD-Score:5.61|P-value:1.86E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:5.24|P-value:5.87E-5 ID:SGTC_177|Compound:1327-0068|FD-Score:9.10|P-value:9.52E-12 ID:SGTC_203|Compound:k066-0004|FD-Score:5.77|P-value:1.11E-5 ID:SGTC_210|Compound:3551-0445|FD-Score:8.38|P-value:3.28E-10 ID:SGTC_214|Compound:4373-3496|FD-Score:5.31|P-value:4.77E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:-4.79|P-value:1.69E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.53|P-value:2.40E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:7.47|P-value:1.83E-8 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.67|P-value:3.03E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:4.43|P-value:5.79E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:6.28|P-value:1.91E-6 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:4.53|P-value:4.39E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.17|P-value:7.26E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:5.42|P-value:3.36E-5 ID:SGTC_608|Compound:k048-0125|FD-Score:4.70|P-value:2.77E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:7.99|P-value:1.90E-9 ID:SGTC_770|Compound:0389-1121|FD-Score:5.00|P-value:1.19E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:9.42|P-value:1.83E-12 ID:SGTC_828|Compound:2074-0507|FD-Score:4.60|P-value:3.65E-4 ID:SGTC_855|Compound:0428-0020|FD-Score:5.53|P-value:2.34E-5 ID:SGTC_894|Compound:0960-0238|FD-Score:6.15|P-value:3.01E-6 ID:SGTC_1001|Compound:4048-4355|FD-Score:4.36|P-value:6.93E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:4.45|P-value:5.39E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.42|P-value:1.16E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:6.31|P-value:1.70E-6 ID:SGTC_1054|Compound:3429-0080|FD-Score:4.75|P-value:2.44E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-4.87|P-value:1.36E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:-4.38|P-value:5.22E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:11.80|P-value:1.59E-18 ID:SGTC_1089|Compound:terbinafine|FD-Score:6.64|P-value:5.12E-7 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.57|P-value:3.91E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:8.69|P-value:7.22E-11 ID:SGTC_1188|Compound:1403-0014|FD-Score:4.78|P-value:2.23E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:5.27|P-value:5.29E-5 ID:SGTC_1306|Compound:1185-0270|FD-Score:10.10|P-value:5.89E-14 ID:SGTC_1633|Compound:st005664|FD-Score:4.35|P-value:7.14E-4 ID:SGTC_1642|Compound:st009582|FD-Score:5.49|P-value:2.73E-5 ID:SGTC_1683|Compound:st010134|FD-Score:4.48|P-value:5.02E-4 ID:SGTC_1724|Compound:st037281|FD-Score:7.54|P-value:1.40E-8 ID:SGTC_1752|Compound:st044819|FD-Score:5.71|P-value:1.32E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:5.98|P-value:5.36E-6 ID:SGTC_1800|Compound:st050079|FD-Score:-5.06|P-value:7.81E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-6.03|P-value:3.37E-6 ID:SGTC_2085|Compound:5307070|FD-Score:5.78|P-value:1.05E-5 ID:SGTC_2135|Compound:5326629|FD-Score:4.73|P-value:2.59E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:5.23|P-value:6.03E-5 ID:SGTC_2268|Compound:7933382|FD-Score:6.28|P-value:1.92E-6 ID:SGTC_2306|Compound:7482397|FD-Score:5.29|P-value:5.07E-5 ID:SGTC_2322|Compound:7413319|FD-Score:5.67|P-value:1.52E-5 ID:SGTC_2336|Compound:9001819|FD-Score:5.74|P-value:1.20E-5 ID:SGTC_2526|Compound:patulin|FD-Score:7.79|P-value:4.62E-9 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:5.27|P-value:5.39E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:-4.54|P-value:3.39E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:7.46|P-value:1.93E-8 ID:SGTC_2737|Compound:bleomycin|FD-Score:8.21|P-value:7.06E-10 ID:SGTC_2743|Compound:auranofin|FD-Score:6.45|P-value:1.02E-6 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-4.69|P-value:2.24E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:17.10|P-value:4.30E-37 ID:SGTC_2801|Compound:7907800|FD-Score:8.40|P-value:2.95E-10 ID:SGTC_3081|Compound:9117243|FD-Score:4.54|P-value:4.27E-4 ID:SGTC_3236|Compound:9132701|FD-Score:6.25|P-value:2.09E-6 ID:SGTC_3282|Compound:9108498|FD-Score:7.52|P-value:1.52E-8 ID:SGTC_3286|Compound:9117135|FD-Score:-5.03|P-value:8.63E-5 ID:SGTC_3292|Compound:9120825|FD-Score:4.46|P-value:5.26E-4 ID:SGTC_3317|Compound:9138495|FD-Score:10.20|P-value:3.05E-14 ID:SGTC_3321|Compound:9137793|FD-Score:6.09|P-value:3.75E-6 ID:SGTC_3326|Compound:9140064|FD-Score:4.42|P-value:5.82E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.58|P-value:3.83E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.85|P-value:1.83E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:4.97|P-value:1.29E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:4.31|P-value:7.81E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:7.04|P-value:1.06E-7 ID:SGTC_159|Compound:3970-0959|FD-Score:7.03|P-value:1.12E-7 ID:SGTC_163|Compound:1327-0036|FD-Score:5.61|P-value:1.86E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:5.24|P-value:5.87E-5 ID:SGTC_177|Compound:1327-0068|FD-Score:9.10|P-value:9.52E-12 ID:SGTC_203|Compound:k066-0004|FD-Score:5.77|P-value:1.11E-5 ID:SGTC_210|Compound:3551-0445|FD-Score:8.38|P-value:3.28E-10 ID:SGTC_214|Compound:4373-3496|FD-Score:5.31|P-value:4.77E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:-4.79|P-value:1.69E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.53|P-value:2.40E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:7.47|P-value:1.83E-8 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.67|P-value:3.03E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:4.43|P-value:5.79E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:6.28|P-value:1.91E-6 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:4.53|P-value:4.39E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.17|P-value:7.26E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:5.42|P-value:3.36E-5 ID:SGTC_608|Compound:k048-0125|FD-Score:4.70|P-value:2.77E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:7.99|P-value:1.90E-9 ID:SGTC_770|Compound:0389-1121|FD-Score:5.00|P-value:1.19E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:9.42|P-value:1.83E-12 ID:SGTC_828|Compound:2074-0507|FD-Score:4.60|P-value:3.65E-4 ID:SGTC_855|Compound:0428-0020|FD-Score:5.53|P-value:2.34E-5 ID:SGTC_894|Compound:0960-0238|FD-Score:6.15|P-value:3.01E-6 ID:SGTC_1001|Compound:4048-4355|FD-Score:4.36|P-value:6.93E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:4.45|P-value:5.39E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.42|P-value:1.16E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:6.31|P-value:1.70E-6 ID:SGTC_1054|Compound:3429-0080|FD-Score:4.75|P-value:2.44E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-4.87|P-value:1.36E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:-4.38|P-value:5.22E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:11.80|P-value:1.59E-18 ID:SGTC_1089|Compound:terbinafine|FD-Score:6.64|P-value:5.12E-7 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.57|P-value:3.91E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:8.69|P-value:7.22E-11 ID:SGTC_1188|Compound:1403-0014|FD-Score:4.78|P-value:2.23E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:5.27|P-value:5.29E-5 ID:SGTC_1306|Compound:1185-0270|FD-Score:10.10|P-value:5.89E-14 ID:SGTC_1633|Compound:st005664|FD-Score:4.35|P-value:7.14E-4 ID:SGTC_1642|Compound:st009582|FD-Score:5.49|P-value:2.73E-5 ID:SGTC_1683|Compound:st010134|FD-Score:4.48|P-value:5.02E-4 ID:SGTC_1724|Compound:st037281|FD-Score:7.54|P-value:1.40E-8 ID:SGTC_1752|Compound:st044819|FD-Score:5.71|P-value:1.32E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:5.98|P-value:5.36E-6 ID:SGTC_1800|Compound:st050079|FD-Score:-5.06|P-value:7.81E-5 ID:SGTC_1818|Compound:st052136|FD-Score:-6.03|P-value:3.37E-6 ID:SGTC_2085|Compound:5307070|FD-Score:5.78|P-value:1.05E-5 ID:SGTC_2135|Compound:5326629|FD-Score:4.73|P-value:2.59E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:5.23|P-value:6.03E-5 ID:SGTC_2268|Compound:7933382|FD-Score:6.28|P-value:1.92E-6 ID:SGTC_2306|Compound:7482397|FD-Score:5.29|P-value:5.07E-5 ID:SGTC_2322|Compound:7413319|FD-Score:5.67|P-value:1.52E-5 ID:SGTC_2336|Compound:9001819|FD-Score:5.74|P-value:1.20E-5 ID:SGTC_2526|Compound:patulin|FD-Score:7.79|P-value:4.62E-9 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:5.27|P-value:5.39E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:-4.54|P-value:3.39E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:7.46|P-value:1.93E-8 ID:SGTC_2737|Compound:bleomycin|FD-Score:8.21|P-value:7.06E-10 ID:SGTC_2743|Compound:auranofin|FD-Score:6.45|P-value:1.02E-6 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:-4.69|P-value:2.24E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:17.10|P-value:4.30E-37 ID:SGTC_2801|Compound:7907800|FD-Score:8.40|P-value:2.95E-10 ID:SGTC_3081|Compound:9117243|FD-Score:4.54|P-value:4.27E-4 ID:SGTC_3236|Compound:9132701|FD-Score:6.25|P-value:2.09E-6 ID:SGTC_3282|Compound:9108498|FD-Score:7.52|P-value:1.52E-8 ID:SGTC_3286|Compound:9117135|FD-Score:-5.03|P-value:8.63E-5 ID:SGTC_3292|Compound:9120825|FD-Score:4.46|P-value:5.26E-4 ID:SGTC_3317|Compound:9138495|FD-Score:10.20|P-value:3.05E-14 ID:SGTC_3321|Compound:9137793|FD-Score:6.09|P-value:3.75E-6 ID:SGTC_3326|Compound:9140064|FD-Score:4.42|P-value:5.82E-4

Top fitness defect scores for YER083C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2776 terbinafine 17.10 19.9 µM 4.30E-37 cell wall signaling 4 3.83E-66
2 SGTC_1060 3448-7350 11.80 82.0 µM 1.59E-18 Golgi 6 2.26E-32
3 SGTC_3317 9138495 10.20 8.8 µM 3.05E-14 3 1.35E-24
4 SGTC_1306 1185-0270 10.10 9.0 µM 5.89E-14 Golgi 12 4.45E-24
5 SGTC_805 3852-0218 9.42 479.0 µM 1.83E-12 ERAD & cell cycle 4 2.26E-21
6 SGTC_177 1327-0068 9.10 8.4 µM 9.52E-12 9 4.49E-20
7 SGTC_1165 k072-0275 8.69 30.3 µM 7.22E-11 5 1.76E-18
8 SGTC_2801 7907800 8.40 81.8 µM 2.95E-10 2 2.26E-17
9 SGTC_210 3551-0445 8.38 56.8 µM 3.28E-10 17 2.73E-17
10 SGTC_2737 bleomycin 8.21 30.5 nM 7.06E-10 Golgi 19 1.10E-16
11 SGTC_733 4069-0007 7.99 56.1 µM 1.90E-9 ERAD & cell cycle 22 6.59E-16
12 SGTC_2526 patulin 7.79 37.6 µM 4.62E-9 16 3.27E-15
13 SGTC_1724 st037281 7.54 8.3 µM 1.40E-8 4 2.41E-14
14 SGTC_3282 9108498 7.52 15.5 µM 1.52E-8 6 2.82E-14
15 SGTC_255 tunicamycin 7.47 200.5 nM 1.83E-8 unfolded protein response 20 3.96E-14
16 SGTC_2736 butenafine 7.46 14.7 µM 1.93E-8 3 4.35E-14
17 SGTC_132 0417-1715 7.04 25.8 µM 1.06E-7 RPP1 & pyrimidine depletion 1 9.43E-13
18 SGTC_159 3970-0959 7.03 50.0 µM 1.12E-7 16 1.04E-12
19 SGTC_1089 terbinafine 6.64 2.2 µM 5.12E-7 3 1.61E-11
20 SGTC_2743 auranofin 6.45 4.3 µM 1.02E-6 RPP1 & pyrimidine depletion 9 5.59E-11

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.267 7.40E-56 YDL100C GET3 hom Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
0.258 3.91E-52 YGL020C GET1 hom Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
0.225 6.39E-40 YNL287W SEC21 het Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
0.210 9.22E-35 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.183 8.88E-27 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.182 1.71E-26 YGR175C ERG1 het Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
0.182 2.73E-26 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.178 1.94E-25 YLR268W SEC22 hom R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
0.170 4.50E-23 YDR108W TRS85 hom Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
0.164 9.45E-22 YBR164C ARL1 hom Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
0.159 1.71E-20 YJL004C SYS1 hom Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.154 2.21E-19 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.154 2.97E-19 YML071C COG8 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.153 5.22E-19 YDR439W LRS4 hom Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
0.153 6.19E-19 YDL195W SEC31 het Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance