HIPHOP chemogenomics database

YER094C / PUP3

Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10

Zygosity: Heterozygous strain

fixedexpanded
Profile for YER094C / PUP3

Click on Significant Values for Screen Details

Top fitness defect scores for YER094C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_64	000l-0018	23.30	272.1 µM	6.87E-74	ubiquinone biosynthesis & proteasome	1	2.13E-120	2.27
2	SGTC_2250	alverine citrate	11.20	130.0 µM	1.79E-18		7	3.16E-29	0.28
3	SGTC_137	0438-0332	9.34	85.4 µM	1.76E-13	ubiquinone biosynthesis & proteasome	10	4.81E-21	1.43
4	SGTC_1597	7-benzyloxygramine	9.06	47.5 µM	8.84E-13	calcium & mitochondrial duress	20	6.77E-20	0.71
5	SGTC_467	ro 31-8220	7.20	72.8 µM	1.04E-8	calcium & mitochondrial duress	4	3.02E-13	0.75
6	SGTC_2249	haloperidol	6.62	116.4 µM	1.29E-7	ergosterol depletion effects on membrane	13	1.84E-11	0.04
7	SGTC_36	0335-0881	6.59	14.2 µM	1.41E-7	ubiquinone biosynthesis & proteasome	11	2.14E-11	0.06
8	SGTC_582	0987-0078	6.55	156.0 µM	1.72E-7	calcium & mitochondrial duress	3	2.94E-11	0.61
9	SGTC_187	1488-2104	6.52	32.7 µM	1.89E-7	calcium & mitochondrial duress	3	3.44E-11	0.91
10	SGTC_320	4092-0846	5.91	5.3 µM	2.11E-6	ergosterol depletion effects on membrane	9	1.73E-9	0.01
11	SGTC_2291	7917025	5.88	104.3 µM	2.37E-6	iron homeostasis	2	2.09E-9	0.33
12	SGTC_312	4092-0653	5.75	6.8 µM	3.79E-6	ergosterol depletion effects on membrane	15	4.48E-9	0.13
13	SGTC_528	3492-0019	5.69	172.0 µM	4.78E-6	calcium & mitochondrial duress	10	6.52E-9	0.00
14	SGTC_2580	diallyl disulphide	5.44	81.4 µM	1.14E-5		1	2.67E-8	0.46
15	SGTC_1880	MG-132	5.44	525.0 µM	1.14E-5		12	2.68E-8	0.01
16	SGTC_398	bronopol	5.37	33.3 µM	1.47E-5	RNA processing & uracil transport	2	4.01E-8	0.69
17	SGTC_2947	9072639	5.36	7.7 µM	1.51E-5	tubulin folding & SWR complex	5	4.20E-8	0.41
18	SGTC_574	1315-0397	5.16	40.0 µM	2.90E-5		4	1.21E-7	0.56
19	SGTC_1381	2324-0066	4.92	62.1 µM	6.47E-5		2	4.41E-7	0.15
20	SGTC_635	k038-0028	4.90	24.7 µM	6.71E-5	ubiquinone biosynthesis & proteasome	7	4.67E-7	0.04

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.581	4.21E-302	YMR314W	PRE5	het	Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.486	1.14E-198	YFR050C	PRE4	het	Beta 7 subunit of the 20S proteasome
0.479	6.74E-192	YOL038W	PRE6	het	Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.478	2.68E-191	YOR362C	PRE10	het	Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.478	3.57E-191	YGL011C	SCL1	het	Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.469	2.22E-183	YGR253C	PUP2	het	Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.468	9.67E-182	YML092C	PRE8	het	Alpha 2 subunit of the 20S proteasome
0.426	8.23E-148	YBL041W	PRE7	het	Beta 6 subunit of the 20S proteasome
0.390	4.45E-122	YJL001W	PRE3	het	Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.374	3.80E-112	YER012W	PRE1	het	Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.370	4.89E-109	YLR021W	IRC25	hom	Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.318	7.94E-80	YNL111C	CYB5	hom	Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.289	1.97E-65	YPR146C_d	YPR146C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.282	2.16E-62	YPR103W	PRE2	het	Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.280	1.36E-61	YPL144W	POC4	hom	Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions