YER099C / PRS2

5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YER099C / PRS2

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:3.31|P-value:6.07E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:4.69|P-value:2.63E-6 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.90|P-value:1.34E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:4.34|P-value:1.24E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:4.98|P-value:6.87E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:3.34|P-value:5.51E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.39|P-value:4.54E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.51|P-value:3.02E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.95|P-value:5.86E-5 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:-4.09|P-value:6.48E-5 ID:SGTC_732|Compound:3970-1727|FD-Score:5.22|P-value:2.10E-7 ID:SGTC_873|Compound:0591-4978|FD-Score:3.17|P-value:9.54E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.37|P-value:4.93E-4 ID:SGTC_949|Compound:1165-0505|FD-Score:3.42|P-value:4.10E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:3.31|P-value:6.10E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.32|P-value:5.79E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:5.15|P-value:2.91E-7 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.66|P-value:1.79E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.23|P-value:7.81E-4 ID:SGTC_1531|Compound:st059297|FD-Score:3.61|P-value:2.09E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:-3.54|P-value:4.89E-4 ID:SGTC_1632|Compound:st005650|FD-Score:-3.95|P-value:1.10E-4 ID:SGTC_1755|Compound:st041960|FD-Score:3.64|P-value:1.89E-4 ID:SGTC_1884|Compound:5633444|FD-Score:3.78|P-value:1.13E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:8.81|P-value:1.08E-17 ID:SGTC_1993|Compound:st073995|FD-Score:-3.47|P-value:6.11E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.42|P-value:4.15E-4 ID:SGTC_2011|Compound:4012611|FD-Score:5.11|P-value:3.64E-7 ID:SGTC_2012|Compound:4023501|FD-Score:-3.62|P-value:3.60E-4 ID:SGTC_2106|Compound:5357045|FD-Score:-3.67|P-value:3.03E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.49|P-value:3.28E-4 ID:SGTC_2128|Compound:5255449|FD-Score:4.11|P-value:3.14E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-3.61|P-value:3.85E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.15|P-value:2.71E-5 ID:SGTC_2390|Compound:9097792|FD-Score:3.76|P-value:1.21E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.51|P-value:3.00E-4 ID:SGTC_2445|Compound:5742597|FD-Score:3.16|P-value:9.68E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.78|P-value:2.11E-4 ID:SGTC_2625|Compound:euphol|FD-Score:6.36|P-value:3.95E-10 ID:SGTC_2918|Compound:7977171|FD-Score:3.22|P-value:8.11E-4 ID:SGTC_3175|Compound:9106517|FD-Score:-3.33|P-value:9.72E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:3.31|P-value:6.07E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:4.69|P-value:2.63E-6 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.90|P-value:1.34E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:4.34|P-value:1.24E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:4.98|P-value:6.87E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:3.34|P-value:5.51E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.39|P-value:4.54E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.51|P-value:3.02E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.95|P-value:5.86E-5 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:-4.09|P-value:6.48E-5 ID:SGTC_732|Compound:3970-1727|FD-Score:5.22|P-value:2.10E-7 ID:SGTC_873|Compound:0591-4978|FD-Score:3.17|P-value:9.54E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.37|P-value:4.93E-4 ID:SGTC_949|Compound:1165-0505|FD-Score:3.42|P-value:4.10E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:3.31|P-value:6.10E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.32|P-value:5.79E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:5.15|P-value:2.91E-7 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.66|P-value:1.79E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.23|P-value:7.81E-4 ID:SGTC_1531|Compound:st059297|FD-Score:3.61|P-value:2.09E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:-3.54|P-value:4.89E-4 ID:SGTC_1632|Compound:st005650|FD-Score:-3.95|P-value:1.10E-4 ID:SGTC_1755|Compound:st041960|FD-Score:3.64|P-value:1.89E-4 ID:SGTC_1884|Compound:5633444|FD-Score:3.78|P-value:1.13E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:8.81|P-value:1.08E-17 ID:SGTC_1993|Compound:st073995|FD-Score:-3.47|P-value:6.11E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.42|P-value:4.15E-4 ID:SGTC_2011|Compound:4012611|FD-Score:5.11|P-value:3.64E-7 ID:SGTC_2012|Compound:4023501|FD-Score:-3.62|P-value:3.60E-4 ID:SGTC_2106|Compound:5357045|FD-Score:-3.67|P-value:3.03E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.49|P-value:3.28E-4 ID:SGTC_2128|Compound:5255449|FD-Score:4.11|P-value:3.14E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-3.61|P-value:3.85E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.15|P-value:2.71E-5 ID:SGTC_2390|Compound:9097792|FD-Score:3.76|P-value:1.21E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.51|P-value:3.00E-4 ID:SGTC_2445|Compound:5742597|FD-Score:3.16|P-value:9.68E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.78|P-value:2.11E-4 ID:SGTC_2625|Compound:euphol|FD-Score:6.36|P-value:3.95E-10 ID:SGTC_2918|Compound:7977171|FD-Score:3.22|P-value:8.11E-4 ID:SGTC_3175|Compound:9106517|FD-Score:-3.33|P-value:9.72E-4

Top fitness defect scores for YER099C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1926 methyl fluorone black 8.81 53.6 µM 1.08E-17 DNA intercalators 6 6.51E-19
2 SGTC_2625 euphol 6.36 100.0 µM 3.95E-10 4 1.03E-10
3 SGTC_732 3970-1727 5.22 52.7 µM 2.10E-7 2 9.03E-8
4 SGTC_1214 0358-0031 5.15 63.7 µM 2.91E-7 4 1.29E-7
5 SGTC_2011 4012611 5.11 177.0 µM 3.64E-7 mitochondrial processes 10 1.64E-7
6 SGTC_184 1494-0393 4.98 24.7 µM 6.87E-7 31 3.25E-7
7 SGTC_42 0133-0086 4.69 60.6 µM 2.63E-6 9 1.38E-6
8 SGTC_131 0141-0277 4.34 33.9 µM 1.24E-5 28 7.27E-6
9 SGTC_2336 9001819 4.15 198.7 µM 2.71E-5 heme biosynthesis & mitochondrial translocase 46 1.69E-5
10 SGTC_2128 5255449 4.11 168.5 µM 3.14E-5 31 1.98E-5
11 SGTC_264 promethazine 3.95 12.5 mM 5.86E-5 DNA intercalators 54 3.86E-5
12 SGTC_1884 5633444 3.78 25.0 µM 1.13E-4 redox potentiating 59 7.81E-5
13 SGTC_2390 9097792 3.76 176.6 µM 1.21E-4 19 8.42E-5
14 SGTC_1260 0700-1617 3.66 7.8 µM 1.79E-4 calcium & mitochondrial duress 37 1.28E-4
15 SGTC_1755 st041960 3.64 45.2 µM 1.89E-4 33 1.36E-4
16 SGTC_1531 st059297 3.61 52.4 µM 2.09E-4 29 1.51E-4
17 SGTC_2395 5646831 3.51 180.2 µM 3.00E-4 43 2.22E-4
18 SGTC_261 5-fluorouridine 3.51 15.4 µM 3.02E-4 exosome 25 2.24E-4
19 SGTC_2116 5511452 3.49 16.9 µM 3.28E-4 24 2.44E-4
20 SGTC_949 1165-0505 3.42 146.0 µM 4.10E-4 36 3.10E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.184 5.92E-27 YDR250C_d YDR250C_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
0.175 1.79E-24 YPL035C_d YPL035C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene
0.168 1.54E-22 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.164 1.37E-21 YGR070W ROM1 hom GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
0.160 1.01E-20 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.159 2.20E-20 YPL197C_d YPL197C_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B
0.154 2.73E-19 YOL035C_d YOL035C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.150 2.31E-18 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.146 1.66E-17 YPR059C_d YPR059C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
0.143 7.97E-17 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.141 3.18E-16 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.136 2.68E-15 YEL032W MCM3 het Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
0.135 3.77E-15 YMR286W MRPL33 hom Mitochondrial ribosomal protein of the large subunit
0.135 5.37E-15 YGL075C MPS2 het Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p
0.130 5.07E-14 YIL144W TID3 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering