YER101C / AST2

Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YER101C / AST2

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:3.47|P-value:2.46E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:3.18|P-value:7.14E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.14|P-value:8.17E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.84|P-value:5.75E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.57|P-value:2.11E-6 ID:SGTC_281|Compound:1672-3440|FD-Score:3.22|P-value:6.19E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.64|P-value:1.26E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:-3.43|P-value:2.37E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-5.70|P-value:3.58E-9 ID:SGTC_380|Compound:3970-0968|FD-Score:-3.79|P-value:5.82E-5 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.18|P-value:6.93E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:3.47|P-value:2.49E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:4.47|P-value:3.47E-6 ID:SGTC_804|Compound:1595-0054|FD-Score:4.54|P-value:2.42E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:3.86|P-value:5.28E-5 ID:SGTC_990|Compound:3985-0011|FD-Score:10.20|P-value:2.55E-25 ID:SGTC_995|Compound:3931-2021|FD-Score:3.87|P-value:5.08E-5 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.28|P-value:4.97E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.12|P-value:1.70E-5 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.06|P-value:9.22E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:-3.91|P-value:3.54E-5 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.80|P-value:6.65E-5 ID:SGTC_1062|Compound:mesoridazine|FD-Score:3.14|P-value:7.99E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:8.62|P-value:1.77E-18 ID:SGTC_1109|Compound:tnp00067|FD-Score:3.31|P-value:4.44E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:5.87|P-value:1.63E-9 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:4.02|P-value:2.61E-5 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.17|P-value:7.24E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:3.22|P-value:6.19E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:-3.19|P-value:5.72E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.60|P-value:1.52E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.44|P-value:2.73E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.68|P-value:1.07E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.17|P-value:7.35E-4 ID:SGTC_1655|Compound:st012546|FD-Score:-3.21|P-value:5.44E-4 ID:SGTC_1660|Compound:st012960|FD-Score:3.47|P-value:2.46E-4 ID:SGTC_1826|Compound:st055655|FD-Score:7.42|P-value:3.76E-14 ID:SGTC_1834|Compound:st055981|FD-Score:3.51|P-value:2.07E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-4.31|P-value:5.73E-6 ID:SGTC_2035|Compound:5154463|FD-Score:3.74|P-value:8.47E-5 ID:SGTC_2049|Compound:5262831|FD-Score:-3.04|P-value:9.76E-4 ID:SGTC_2195|Compound:6536160|FD-Score:3.56|P-value:1.74E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.64|P-value:1.28E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:4.10|P-value:1.86E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:6.42|P-value:4.94E-11 ID:SGTC_2592|Compound:piperlongumine|FD-Score:8.35|P-value:1.89E-17 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:10.10|P-value:8.03E-25 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.32|P-value:4.23E-4 ID:SGTC_2655|Compound:perillic acid|FD-Score:-3.10|P-value:7.98E-4 ID:SGTC_2986|Compound:9022445|FD-Score:4.15|P-value:1.50E-5 ID:SGTC_3069|Compound:9117009|FD-Score:3.59|P-value:1.53E-4 ID:SGTC_3099|Compound:9120367|FD-Score:-3.13|P-value:7.12E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.58|P-value:1.61E-4 ID:SGTC_3208|Compound:9114115|FD-Score:7.47|P-value:2.56E-14 ID:SGTC_3209|Compound:9113616|FD-Score:3.45|P-value:2.66E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.37|P-value:3.01E-4 ID:SGTC_3236|Compound:9132701|FD-Score:4.02|P-value:2.66E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:3.47|P-value:2.46E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:3.18|P-value:7.14E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.14|P-value:8.17E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.84|P-value:5.75E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.57|P-value:2.11E-6 ID:SGTC_281|Compound:1672-3440|FD-Score:3.22|P-value:6.19E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.64|P-value:1.26E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:-3.43|P-value:2.37E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-5.70|P-value:3.58E-9 ID:SGTC_380|Compound:3970-0968|FD-Score:-3.79|P-value:5.82E-5 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.18|P-value:6.93E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:3.47|P-value:2.49E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:4.47|P-value:3.47E-6 ID:SGTC_804|Compound:1595-0054|FD-Score:4.54|P-value:2.42E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:3.86|P-value:5.28E-5 ID:SGTC_990|Compound:3985-0011|FD-Score:10.20|P-value:2.55E-25 ID:SGTC_995|Compound:3931-2021|FD-Score:3.87|P-value:5.08E-5 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.28|P-value:4.97E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.12|P-value:1.70E-5 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.06|P-value:9.22E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:-3.91|P-value:3.54E-5 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.80|P-value:6.65E-5 ID:SGTC_1062|Compound:mesoridazine|FD-Score:3.14|P-value:7.99E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:8.62|P-value:1.77E-18 ID:SGTC_1109|Compound:tnp00067|FD-Score:3.31|P-value:4.44E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:5.87|P-value:1.63E-9 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:4.02|P-value:2.61E-5 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.17|P-value:7.24E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:3.22|P-value:6.19E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:-3.19|P-value:5.72E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.60|P-value:1.52E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.44|P-value:2.73E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.68|P-value:1.07E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.17|P-value:7.35E-4 ID:SGTC_1655|Compound:st012546|FD-Score:-3.21|P-value:5.44E-4 ID:SGTC_1660|Compound:st012960|FD-Score:3.47|P-value:2.46E-4 ID:SGTC_1826|Compound:st055655|FD-Score:7.42|P-value:3.76E-14 ID:SGTC_1834|Compound:st055981|FD-Score:3.51|P-value:2.07E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-4.31|P-value:5.73E-6 ID:SGTC_2035|Compound:5154463|FD-Score:3.74|P-value:8.47E-5 ID:SGTC_2049|Compound:5262831|FD-Score:-3.04|P-value:9.76E-4 ID:SGTC_2195|Compound:6536160|FD-Score:3.56|P-value:1.74E-4 ID:SGTC_2395|Compound:5646831|FD-Score:3.64|P-value:1.28E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:4.10|P-value:1.86E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:6.42|P-value:4.94E-11 ID:SGTC_2592|Compound:piperlongumine|FD-Score:8.35|P-value:1.89E-17 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:10.10|P-value:8.03E-25 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.32|P-value:4.23E-4 ID:SGTC_2655|Compound:perillic acid|FD-Score:-3.10|P-value:7.98E-4 ID:SGTC_2986|Compound:9022445|FD-Score:4.15|P-value:1.50E-5 ID:SGTC_3069|Compound:9117009|FD-Score:3.59|P-value:1.53E-4 ID:SGTC_3099|Compound:9120367|FD-Score:-3.13|P-value:7.12E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.58|P-value:1.61E-4 ID:SGTC_3208|Compound:9114115|FD-Score:7.47|P-value:2.56E-14 ID:SGTC_3209|Compound:9113616|FD-Score:3.45|P-value:2.66E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-3.37|P-value:3.01E-4 ID:SGTC_3236|Compound:9132701|FD-Score:4.02|P-value:2.66E-5

Top fitness defect scores for YER101C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_990 3985-0011 10.20 41.3 µM 2.55E-25 amide catabolism 2 6.70E-25
2 SGTC_2595 hexamethylquercetagetin 10.10 100.0 µM 8.03E-25 1 2.06E-24
3 SGTC_1095 3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran 8.62 15.5 µM 1.77E-18 2 3.41E-18
4 SGTC_2592 piperlongumine 8.35 83.3 µM 1.89E-17 TSC3-RPN4 2 3.48E-17
5 SGTC_3208 9114115 7.47 49.5 µM 2.56E-14 4 4.10E-14
6 SGTC_1826 st055655 7.42 41.6 µM 3.76E-14 ERG2 10 5.97E-14
7 SGTC_2574 2',4'-dihydroxychalcone 6.42 14.6 µM 4.94E-11 4 6.85E-11
8 SGTC_1113 artemisinin 5.87 1.2 µM 1.63E-9 heme biosynthesis & mitochondrial translocase 3 2.12E-9
9 SGTC_243 5hr heat shock (37°C) + splitomicin 4.57 1.0 µM 2.11E-6 heat shock/prefoldin 33 2.42E-6
10 SGTC_804 1595-0054 4.54 20.9 µM 2.42E-6 sphingolipid biosynthesis & PDR1 23 2.77E-6
11 SGTC_803 4488-1585 4.47 147.0 µM 3.47E-6 8 3.94E-6
12 SGTC_2986 9022445 4.15 71.4 µM 1.50E-5 10 1.66E-5
13 SGTC_1002 4049-0206 4.12 172.0 µM 1.70E-5 25 1.88E-5
14 SGTC_2543 pimpinellin 4.10 60.7 µM 1.86E-5 Golgi 12 2.05E-5
15 SGTC_1135 dihydrocapsaicin 4.02 536.7 nM 2.61E-5 34 2.86E-5
16 SGTC_3236 9132701 4.02 49.5 µM 2.66E-5 TSC3-RPN4 25 2.92E-5
17 SGTC_995 3931-2021 3.87 34.6 µM 5.08E-5 RPP1 & pyrimidine depletion 24 5.52E-5
18 SGTC_910 3454-3003 3.86 490.0 µM 5.28E-5 16 5.73E-5
19 SGTC_153 4077-0274 3.84 519.7 µM 5.75E-5 15 6.24E-5
20 SGTC_1029 k015-0030 3.80 64.7 µM 6.65E-5 30 7.20E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.109 2.38E-10 YGR177C ATF2 hom Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking
0.109 2.53E-10 YOL032W OPI10 hom Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress
0.101 4.80E-9 YPR201W ARR3 hom Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite
0.100 7.72E-9 YOR222W ODC2 hom Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism
0.097 2.01E-8 YER157W COG3 het Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.093 7.94E-8 YPL147W PXA1 hom Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder
0.090 1.64E-7 YHR175W-A_p YHR175W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.084 1.06E-6 YBR216C YBP1 hom Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication
0.083 1.33E-6 YML065W ORC1 het Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity
0.083 1.36E-6 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.082 1.97E-6 YGL210W YPT32 hom Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication
0.082 2.11E-6 YKL204W EAP1 hom eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade
0.082 2.29E-6 YJL200C ACO2 hom Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol
0.080 3.73E-6 YHR134W WSS1 hom Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response
0.078 5.62E-6 YMR240C CUS1 het Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p