YER116C / SLX8

Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YER116C / SLX8

Click on Significant Values for Screen Details ID:SGTC_48|Compound:0382-0018|FD-Score:4.28|P-value:8.99E-6 ID:SGTC_131|Compound:0141-0277|FD-Score:4.06|P-value:2.25E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:3.16|P-value:6.82E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:5.11|P-value:1.75E-7 ID:SGTC_712|Compound:r015-0009|FD-Score:3.68|P-value:1.05E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:-3.38|P-value:8.30E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:-3.45|P-value:6.55E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.63|P-value:4.95E-8 ID:SGTC_1021|Compound:4476-0760|FD-Score:4.59|P-value:2.15E-6 ID:SGTC_1023|Compound:4482-3888|FD-Score:-3.69|P-value:2.89E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:3.29|P-value:4.34E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.16|P-value:6.79E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:-3.71|P-value:2.71E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:4.78|P-value:8.79E-7 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.50|P-value:2.04E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:4.37|P-value:5.83E-6 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.21|P-value:1.19E-5 ID:SGTC_1454|Compound:4239-0054|FD-Score:-3.67|P-value:3.04E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:4.40|P-value:5.19E-6 ID:SGTC_1664|Compound:st014217|FD-Score:3.09|P-value:8.58E-4 ID:SGTC_1704|Compound:st028805|FD-Score:3.96|P-value:3.40E-5 ID:SGTC_1707|Compound:st032323|FD-Score:4.14|P-value:1.64E-5 ID:SGTC_1730|Compound:st033808|FD-Score:3.55|P-value:1.70E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-3.57|P-value:4.38E-4 ID:SGTC_1732|Compound:st034308|FD-Score:5.75|P-value:5.44E-9 ID:SGTC_1750|Compound:st081859|FD-Score:3.66|P-value:1.13E-4 ID:SGTC_1809|Compound:st050081|FD-Score:3.23|P-value:5.36E-4 ID:SGTC_1845|Compound:st056753|FD-Score:4.09|P-value:1.99E-5 ID:SGTC_1969|Compound:st069479|FD-Score:-4.04|P-value:7.85E-5 ID:SGTC_1983|Compound:st071813|FD-Score:3.65|P-value:1.17E-4 ID:SGTC_2364|Compound:9055074|FD-Score:3.74|P-value:8.15E-5 ID:SGTC_2378|Compound:9074213|FD-Score:4.24|P-value:1.05E-5 ID:SGTC_2385|Compound:9074692|FD-Score:4.02|P-value:2.73E-5 ID:SGTC_2397|Compound:5161689|FD-Score:3.06|P-value:9.33E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.13|P-value:7.50E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.09|P-value:8.60E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:3.16|P-value:6.65E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-3.52|P-value:5.25E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:-3.74|P-value:2.38E-4 ID:SGTC_2832|Compound:7992762|FD-Score:5.82|P-value:3.58E-9 ID:SGTC_2854|Compound:9024559|FD-Score:3.95|P-value:3.58E-5 ID:SGTC_2971|Compound:9092186|FD-Score:-4.11|P-value:5.80E-5 ID:SGTC_2972|Compound:9093619|FD-Score:4.92|P-value:4.44E-7 ID:SGTC_3023|Compound:9089636|FD-Score:3.35|P-value:3.55E-4 ID:SGTC_3214|Compound:9129694|FD-Score:-3.35|P-value:9.22E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-3.38|P-value:8.32E-4 ID:SGTC_3311|Compound:9131146|FD-Score:-3.49|P-value:5.81E-4 ID:SGTC_3318|Compound:9139014|FD-Score:3.74|P-value:8.22E-5 ID:SGTC_3329|Compound:9141233|FD-Score:-3.48|P-value:6.08E-4 ID:SGTC_3332|Compound:9141069|FD-Score:3.65|P-value:1.15E-4 ID:SGTC_3333|Compound:9141820|FD-Score:6.32|P-value:1.83E-10 ID:SGTC_48|Compound:0382-0018|FD-Score:4.28|P-value:8.99E-6 ID:SGTC_131|Compound:0141-0277|FD-Score:4.06|P-value:2.25E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:3.16|P-value:6.82E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:5.11|P-value:1.75E-7 ID:SGTC_712|Compound:r015-0009|FD-Score:3.68|P-value:1.05E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:-3.38|P-value:8.30E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:-3.45|P-value:6.55E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.63|P-value:4.95E-8 ID:SGTC_1021|Compound:4476-0760|FD-Score:4.59|P-value:2.15E-6 ID:SGTC_1023|Compound:4482-3888|FD-Score:-3.69|P-value:2.89E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:3.29|P-value:4.34E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.16|P-value:6.79E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:-3.71|P-value:2.71E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:4.78|P-value:8.79E-7 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.50|P-value:2.04E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:4.37|P-value:5.83E-6 ID:SGTC_1450|Compound:4112-4260|FD-Score:4.21|P-value:1.19E-5 ID:SGTC_1454|Compound:4239-0054|FD-Score:-3.67|P-value:3.04E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:4.40|P-value:5.19E-6 ID:SGTC_1664|Compound:st014217|FD-Score:3.09|P-value:8.58E-4 ID:SGTC_1704|Compound:st028805|FD-Score:3.96|P-value:3.40E-5 ID:SGTC_1707|Compound:st032323|FD-Score:4.14|P-value:1.64E-5 ID:SGTC_1730|Compound:st033808|FD-Score:3.55|P-value:1.70E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-3.57|P-value:4.38E-4 ID:SGTC_1732|Compound:st034308|FD-Score:5.75|P-value:5.44E-9 ID:SGTC_1750|Compound:st081859|FD-Score:3.66|P-value:1.13E-4 ID:SGTC_1809|Compound:st050081|FD-Score:3.23|P-value:5.36E-4 ID:SGTC_1845|Compound:st056753|FD-Score:4.09|P-value:1.99E-5 ID:SGTC_1969|Compound:st069479|FD-Score:-4.04|P-value:7.85E-5 ID:SGTC_1983|Compound:st071813|FD-Score:3.65|P-value:1.17E-4 ID:SGTC_2364|Compound:9055074|FD-Score:3.74|P-value:8.15E-5 ID:SGTC_2378|Compound:9074213|FD-Score:4.24|P-value:1.05E-5 ID:SGTC_2385|Compound:9074692|FD-Score:4.02|P-value:2.73E-5 ID:SGTC_2397|Compound:5161689|FD-Score:3.06|P-value:9.33E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.13|P-value:7.50E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.09|P-value:8.60E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:3.16|P-value:6.65E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-3.52|P-value:5.25E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:-3.74|P-value:2.38E-4 ID:SGTC_2832|Compound:7992762|FD-Score:5.82|P-value:3.58E-9 ID:SGTC_2854|Compound:9024559|FD-Score:3.95|P-value:3.58E-5 ID:SGTC_2971|Compound:9092186|FD-Score:-4.11|P-value:5.80E-5 ID:SGTC_2972|Compound:9093619|FD-Score:4.92|P-value:4.44E-7 ID:SGTC_3023|Compound:9089636|FD-Score:3.35|P-value:3.55E-4 ID:SGTC_3214|Compound:9129694|FD-Score:-3.35|P-value:9.22E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-3.38|P-value:8.32E-4 ID:SGTC_3311|Compound:9131146|FD-Score:-3.49|P-value:5.81E-4 ID:SGTC_3318|Compound:9139014|FD-Score:3.74|P-value:8.22E-5 ID:SGTC_3329|Compound:9141233|FD-Score:-3.48|P-value:6.08E-4 ID:SGTC_3332|Compound:9141069|FD-Score:3.65|P-value:1.15E-4 ID:SGTC_3333|Compound:9141820|FD-Score:6.32|P-value:1.83E-10

Top fitness defect scores for YER116C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3333 9141820 6.32 12.1 µM 1.83E-10 2 1.34E-10
2 SGTC_2832 7992762 5.82 52.0 µM 3.58E-9 7 2.93E-9
3 SGTC_1732 st034308 5.75 51.8 µM 5.44E-9 3 4.52E-9
4 SGTC_504 lovastatin 5.11 124.0 µM 1.75E-7 5 1.63E-7
5 SGTC_2972 9093619 4.92 59.9 µM 4.44E-7 8 4.28E-7
6 SGTC_1414 3917-0015 4.78 5.4 µM 8.79E-7 4 8.67E-7
7 SGTC_1021 4476-0760 4.59 85.3 µM 2.15E-6 12 2.18E-6
8 SGTC_1500 4488-1866 4.40 171.0 µM 5.19E-6 RPP1 & pyrimidine depletion 8 5.39E-6
9 SGTC_1446 4239-0190 4.37 62.0 µM 5.83E-6 28 6.08E-6
10 SGTC_48 0382-0018 4.28 55.0 µM 8.99E-6 2 9.48E-6
11 SGTC_2378 9074213 4.24 121.4 µM 1.05E-5 RSC complex & mRNA processing 11 1.11E-5
12 SGTC_1450 4112-4260 4.21 37.6 µM 1.19E-5 calcium & mitochondrial duress 22 1.27E-5
13 SGTC_1707 st032323 4.14 6.3 µM 1.64E-5 DNA damage response 49 1.75E-5
14 SGTC_1845 st056753 4.09 3.0 µM 1.99E-5 TSC3-RPN4 33 2.14E-5
15 SGTC_131 0141-0277 4.06 33.9 µM 2.25E-5 33 2.43E-5
16 SGTC_2385 9074692 4.02 200.0 µM 2.73E-5 42 2.97E-5
17 SGTC_1704 st028805 3.96 70.8 µM 3.40E-5 9 3.72E-5
18 SGTC_2854 9024559 3.95 71.4 µM 3.58E-5 21 3.92E-5
19 SGTC_2364 9055074 3.74 200.0 µM 8.15E-5 25 9.11E-5
20 SGTC_3318 9139014 3.74 38.6 µM 8.22E-5 19 9.18E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.099 9.84E-9 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.097 1.69E-8 YER176W ECM32 hom DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
0.094 5.63E-8 YLL018C-A COX19 hom Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.091 1.25E-7 YPR099C_d YPR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W
0.083 1.51E-6 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.081 2.84E-6 YPR153W YPR153W hom Putative protein of unknown function
0.081 2.89E-6 YPR109W_p YPR109W_p hom Predicted membrane protein; dipoid deletion strain has high budding index
0.080 3.58E-6 YAR007C RFA1 het Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
0.080 3.96E-6 YLR060W FRS1 het Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
0.078 5.52E-6 YHR154W RTT107 hom Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
0.076 1.06E-5 YJR035W RAD26 hom Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein
0.076 1.11E-5 YER003C PMI40 het Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
0.075 1.37E-5 YKL053C-A MDM35 hom Mitochondrial intermembrane space protein; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
0.075 1.52E-5 YOR270C VPH1 hom Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress
0.073 2.24E-5 YGR034W RPL26B hom Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication