YER120W / SCS2

Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YER120W / SCS2

Click on Significant Values for Screen Details ID:SGTC_127|Compound:0180-0333|FD-Score:3.25|P-value:4.90E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:3.36|P-value:3.29E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:3.95|P-value:3.11E-5 ID:SGTC_187|Compound:1488-2104|FD-Score:4.00|P-value:2.53E-5 ID:SGTC_197|Compound:4544-0043|FD-Score:3.66|P-value:1.03E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:12.10|P-value:1.38E-34 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.42|P-value:2.59E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:5.95|P-value:8.20E-10 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.78|P-value:6.20E-7 ID:SGTC_313|Compound:3474-0040|FD-Score:7.24|P-value:1.07E-13 ID:SGTC_328|Compound:k915-0120|FD-Score:3.39|P-value:2.98E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:3.43|P-value:2.48E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.24|P-value:5.11E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.73|P-value:4.40E-12 ID:SGTC_447|Compound:ml7|FD-Score:3.56|P-value:1.54E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:6.51|P-value:2.05E-11 ID:SGTC_502|Compound:l-744,832|FD-Score:3.20|P-value:5.92E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:3.98|P-value:2.72E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:6.45|P-value:3.22E-11 ID:SGTC_648|Compound:0375-0473|FD-Score:3.05|P-value:9.83E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.44|P-value:2.44E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:-3.75|P-value:7.47E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:3.53|P-value:1.72E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.46|P-value:2.25E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:-3.29|P-value:4.50E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:4.07|P-value:1.88E-5 ID:SGTC_1136|Compound:pergolide|FD-Score:3.96|P-value:3.00E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:4.03|P-value:2.17E-5 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.48|P-value:2.07E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.06|P-value:1.92E-5 ID:SGTC_1261|Compound:0712-0010|FD-Score:5.27|P-value:4.69E-8 ID:SGTC_1317|Compound:1273-0028|FD-Score:6.19|P-value:1.79E-10 ID:SGTC_1320|Compound:1313-0481|FD-Score:3.59|P-value:1.38E-4 ID:SGTC_1687|Compound:st020778|FD-Score:3.93|P-value:3.31E-5 ID:SGTC_1712|Compound:st032288|FD-Score:11.40|P-value:2.11E-31 ID:SGTC_1760|Compound:st045453|FD-Score:8.08|P-value:1.35E-16 ID:SGTC_1800|Compound:st050079|FD-Score:3.52|P-value:1.77E-4 ID:SGTC_1801|Compound:st050089|FD-Score:5.15|P-value:8.84E-8 ID:SGTC_1803|Compound:st050068|FD-Score:4.55|P-value:2.01E-6 ID:SGTC_1831|Compound:st055328|FD-Score:3.11|P-value:8.09E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.94|P-value:2.81E-7 ID:SGTC_2108|Compound:5365811|FD-Score:3.34|P-value:3.52E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.39|P-value:2.93E-4 ID:SGTC_2397|Compound:5161689|FD-Score:-3.24|P-value:5.24E-4 ID:SGTC_2435|Compound:5706476|FD-Score:4.33|P-value:5.62E-6 ID:SGTC_2442|Compound:5158906|FD-Score:-3.27|P-value:4.83E-4 ID:SGTC_2485|Compound:5633444|FD-Score:6.47|P-value:2.66E-11 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-5.18|P-value:7.89E-8 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:5.58|P-value:7.68E-9 ID:SGTC_2827|Compound:7993847|FD-Score:3.94|P-value:3.19E-5 ID:SGTC_2873|Compound:9040596|FD-Score:-3.24|P-value:5.21E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.13|P-value:7.49E-4 ID:SGTC_2948|Compound:9070189|FD-Score:3.98|P-value:2.70E-5 ID:SGTC_2953|Compound:9074162|FD-Score:4.02|P-value:2.26E-5 ID:SGTC_2986|Compound:9022445|FD-Score:3.16|P-value:6.81E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.70|P-value:8.88E-5 ID:SGTC_3149|Compound:9097855|FD-Score:5.70|P-value:3.78E-9 ID:SGTC_3150|Compound:9097574|FD-Score:4.05|P-value:2.04E-5 ID:SGTC_3154|Compound:9100113|FD-Score:4.25|P-value:8.34E-6 ID:SGTC_3275|Compound:9138069|FD-Score:-4.15|P-value:1.36E-5 ID:SGTC_3311|Compound:9131146|FD-Score:5.35|P-value:2.89E-8 ID:SGTC_3324|Compound:9138759|FD-Score:4.06|P-value:1.95E-5 ID:SGTC_127|Compound:0180-0333|FD-Score:3.25|P-value:4.90E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:3.36|P-value:3.29E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:3.95|P-value:3.11E-5 ID:SGTC_187|Compound:1488-2104|FD-Score:4.00|P-value:2.53E-5 ID:SGTC_197|Compound:4544-0043|FD-Score:3.66|P-value:1.03E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:12.10|P-value:1.38E-34 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.42|P-value:2.59E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:5.95|P-value:8.20E-10 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.78|P-value:6.20E-7 ID:SGTC_313|Compound:3474-0040|FD-Score:7.24|P-value:1.07E-13 ID:SGTC_328|Compound:k915-0120|FD-Score:3.39|P-value:2.98E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:3.43|P-value:2.48E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.24|P-value:5.11E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.73|P-value:4.40E-12 ID:SGTC_447|Compound:ml7|FD-Score:3.56|P-value:1.54E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:6.51|P-value:2.05E-11 ID:SGTC_502|Compound:l-744,832|FD-Score:3.20|P-value:5.92E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:3.98|P-value:2.72E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:6.45|P-value:3.22E-11 ID:SGTC_648|Compound:0375-0473|FD-Score:3.05|P-value:9.83E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:3.44|P-value:2.44E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:-3.75|P-value:7.47E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:3.53|P-value:1.72E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.46|P-value:2.25E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:-3.29|P-value:4.50E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:4.07|P-value:1.88E-5 ID:SGTC_1136|Compound:pergolide|FD-Score:3.96|P-value:3.00E-5 ID:SGTC_1189|Compound:1431-2119|FD-Score:4.03|P-value:2.17E-5 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.48|P-value:2.07E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.06|P-value:1.92E-5 ID:SGTC_1261|Compound:0712-0010|FD-Score:5.27|P-value:4.69E-8 ID:SGTC_1317|Compound:1273-0028|FD-Score:6.19|P-value:1.79E-10 ID:SGTC_1320|Compound:1313-0481|FD-Score:3.59|P-value:1.38E-4 ID:SGTC_1687|Compound:st020778|FD-Score:3.93|P-value:3.31E-5 ID:SGTC_1712|Compound:st032288|FD-Score:11.40|P-value:2.11E-31 ID:SGTC_1760|Compound:st045453|FD-Score:8.08|P-value:1.35E-16 ID:SGTC_1800|Compound:st050079|FD-Score:3.52|P-value:1.77E-4 ID:SGTC_1801|Compound:st050089|FD-Score:5.15|P-value:8.84E-8 ID:SGTC_1803|Compound:st050068|FD-Score:4.55|P-value:2.01E-6 ID:SGTC_1831|Compound:st055328|FD-Score:3.11|P-value:8.09E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.94|P-value:2.81E-7 ID:SGTC_2108|Compound:5365811|FD-Score:3.34|P-value:3.52E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.39|P-value:2.93E-4 ID:SGTC_2397|Compound:5161689|FD-Score:-3.24|P-value:5.24E-4 ID:SGTC_2435|Compound:5706476|FD-Score:4.33|P-value:5.62E-6 ID:SGTC_2442|Compound:5158906|FD-Score:-3.27|P-value:4.83E-4 ID:SGTC_2485|Compound:5633444|FD-Score:6.47|P-value:2.66E-11 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-5.18|P-value:7.89E-8 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:5.58|P-value:7.68E-9 ID:SGTC_2827|Compound:7993847|FD-Score:3.94|P-value:3.19E-5 ID:SGTC_2873|Compound:9040596|FD-Score:-3.24|P-value:5.21E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.13|P-value:7.49E-4 ID:SGTC_2948|Compound:9070189|FD-Score:3.98|P-value:2.70E-5 ID:SGTC_2953|Compound:9074162|FD-Score:4.02|P-value:2.26E-5 ID:SGTC_2986|Compound:9022445|FD-Score:3.16|P-value:6.81E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.70|P-value:8.88E-5 ID:SGTC_3149|Compound:9097855|FD-Score:5.70|P-value:3.78E-9 ID:SGTC_3150|Compound:9097574|FD-Score:4.05|P-value:2.04E-5 ID:SGTC_3154|Compound:9100113|FD-Score:4.25|P-value:8.34E-6 ID:SGTC_3275|Compound:9138069|FD-Score:-4.15|P-value:1.36E-5 ID:SGTC_3311|Compound:9131146|FD-Score:5.35|P-value:2.89E-8 ID:SGTC_3324|Compound:9138759|FD-Score:4.06|P-value:1.95E-5

Top fitness defect scores for YER120W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_221 trifluoperazine 12.10 10.3 µM 1.38E-34 NEO1-PIK1 38 9.30E-34
2 SGTC_1712 st032288 11.40 53.4 µM 2.11E-31 calcium & mitochondrial duress 14 1.19E-30
3 SGTC_1760 st045453 8.08 10.4 µM 1.35E-16 NEO1-PIK1 50 3.25E-16
4 SGTC_313 3474-0040 7.24 5.0 µM 1.07E-13 NEO1-PIK1 15 2.19E-13
5 SGTC_445 e6 berbamine 6.73 66.0 µM 4.40E-12 NEO1-PIK1 18 8.21E-12
6 SGTC_467 ro 31-8220 6.51 72.8 µM 2.05E-11 calcium & mitochondrial duress 27 3.67E-11
7 SGTC_2485 5633444 6.47 17.8 µM 2.66E-11 redox potentiating 9 4.75E-11
8 SGTC_528 3492-0019 6.45 172.0 µM 3.22E-11 calcium & mitochondrial duress 110 5.72E-11
9 SGTC_1317 1273-0028 6.19 910.0 nM 1.79E-10 azole & statin 24 3.04E-10
10 SGTC_266 formosanin C 5.95 406.0 nM 8.20E-10 sphingolipid biosynthesis & PDR1 28 1.34E-9
11 SGTC_3149 9097855 5.70 49.5 µM 3.78E-9 Golgi 7 5.96E-9
12 SGTC_2636 apigenin dimethyl ether 5.58 100.0 µM 7.68E-9 12 1.19E-8
13 SGTC_3311 9131146 5.35 2.9 µM 2.89E-8 cell wall 77 4.34E-8
14 SGTC_1261 0712-0010 5.27 12.7 µM 4.69E-8 RNA processing & uracil transport 7 6.95E-8
15 SGTC_1801 st050089 5.15 28.4 µM 8.84E-8 NEO1-PIK1 70 1.29E-7
16 SGTC_2021 4019055 4.94 173.0 µM 2.81E-7 endomembrane recycling 68 3.98E-7
17 SGTC_275 trichlorophene 4.78 5.7 µM 6.20E-7 mitochondrial stress 185 8.62E-7
18 SGTC_1803 st050068 4.55 52.4 µM 2.01E-6 NEO1-PIK1 137 2.72E-6
19 SGTC_2435 5706476 4.33 10.6 µM 5.62E-6 20 7.40E-6
20 SGTC_3154 9100113 4.25 49.5 µM 8.34E-6 23 1.09E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.436 3.81E-156 YER119C-A_d YER119C-A_d hom Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2
0.417 3.83E-141 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.366 1.15E-106 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.350 1.81E-97 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.335 5.64E-89 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.326 6.51E-84 YAR042W SWH1 hom Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction
0.314 9.14E-78 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.306 8.17E-74 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.305 1.92E-73 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.296 4.57E-69 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.292 4.64E-67 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.291 1.14E-66 YDR492W IZH1 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
0.287 9.50E-65 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.285 8.81E-64 YIL170W HXT12 hom Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family
0.282 2.78E-62 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval