YER123W / YCK3

Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YER123W / YCK3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-3.48|P-value:6.88E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.95|P-value:2.16E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.39|P-value:9.19E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:3.74|P-value:4.43E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:4.45|P-value:3.62E-5 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.74|P-value:4.35E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:4.10|P-value:1.30E-4 ID:SGTC_328|Compound:k915-0120|FD-Score:4.07|P-value:1.43E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:4.03|P-value:1.65E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:-3.43|P-value:8.16E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:4.06|P-value:1.49E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:4.98|P-value:4.23E-6 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.51|P-value:9.05E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:5.52|P-value:3.96E-7 ID:SGTC_738|Compound:1315-0003|FD-Score:5.18|P-value:1.83E-6 ID:SGTC_881|Compound:0833-0569|FD-Score:3.81|P-value:3.44E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:4.47|P-value:3.40E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.56|P-value:7.86E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:5.54|P-value:3.58E-7 ID:SGTC_1164|Compound:k060-0068|FD-Score:-4.03|P-value:1.11E-4 ID:SGTC_1174|Compound:0739-0097|FD-Score:5.16|P-value:1.97E-6 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.52|P-value:8.70E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.84|P-value:3.14E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.94|P-value:2.23E-4 ID:SGTC_1630|Compound:st005209|FD-Score:-3.63|P-value:4.23E-4 ID:SGTC_1657|Compound:st012939|FD-Score:3.53|P-value:8.54E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.12|P-value:1.21E-4 ID:SGTC_1677|Compound:st019204|FD-Score:4.61|P-value:1.93E-5 ID:SGTC_1713|Compound:st033146|FD-Score:3.80|P-value:3.55E-4 ID:SGTC_1723|Compound:st034307|FD-Score:4.49|P-value:3.14E-5 ID:SGTC_1759|Compound:st043059|FD-Score:3.74|P-value:4.31E-4 ID:SGTC_1816|Compound:st051957|FD-Score:4.55|P-value:2.49E-5 ID:SGTC_2002|Compound:4023503|FD-Score:-3.41|P-value:8.45E-4 ID:SGTC_2040|Compound:5100729|FD-Score:4.01|P-value:1.79E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-5.04|P-value:2.00E-6 ID:SGTC_2055|Compound:5235184|FD-Score:6.56|P-value:2.14E-9 ID:SGTC_2208|Compound:7283366|FD-Score:3.57|P-value:7.42E-4 ID:SGTC_2293|Compound:7624732|FD-Score:5.19|P-value:1.79E-6 ID:SGTC_2303|Compound:7774051|FD-Score:3.58|P-value:7.22E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:5.90|P-value:6.60E-8 ID:SGTC_2796|Compound:7949634|FD-Score:-4.70|P-value:8.38E-6 ID:SGTC_2822|Compound:7994718|FD-Score:3.71|P-value:4.86E-4 ID:SGTC_2826|Compound:7992541|FD-Score:4.55|P-value:2.44E-5 ID:SGTC_2903|Compound:5923464|FD-Score:5.05|P-value:3.16E-6 ID:SGTC_2992|Compound:9074591|FD-Score:3.50|P-value:9.25E-4 ID:SGTC_2995|Compound:9067767|FD-Score:-5.47|P-value:2.91E-7 ID:SGTC_2997|Compound:9067075|FD-Score:-4.09|P-value:8.78E-5 ID:SGTC_2999|Compound:9069765|FD-Score:-4.20|P-value:5.94E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.48|P-value:6.88E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.95|P-value:2.16E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.39|P-value:9.19E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:3.74|P-value:4.43E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:4.45|P-value:3.62E-5 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.74|P-value:4.35E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:4.10|P-value:1.30E-4 ID:SGTC_328|Compound:k915-0120|FD-Score:4.07|P-value:1.43E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:4.03|P-value:1.65E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:-3.43|P-value:8.16E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:4.06|P-value:1.49E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:4.98|P-value:4.23E-6 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.51|P-value:9.05E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:5.52|P-value:3.96E-7 ID:SGTC_738|Compound:1315-0003|FD-Score:5.18|P-value:1.83E-6 ID:SGTC_881|Compound:0833-0569|FD-Score:3.81|P-value:3.44E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:4.47|P-value:3.40E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.56|P-value:7.86E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:5.54|P-value:3.58E-7 ID:SGTC_1164|Compound:k060-0068|FD-Score:-4.03|P-value:1.11E-4 ID:SGTC_1174|Compound:0739-0097|FD-Score:5.16|P-value:1.97E-6 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.52|P-value:8.70E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.84|P-value:3.14E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.94|P-value:2.23E-4 ID:SGTC_1630|Compound:st005209|FD-Score:-3.63|P-value:4.23E-4 ID:SGTC_1657|Compound:st012939|FD-Score:3.53|P-value:8.54E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.12|P-value:1.21E-4 ID:SGTC_1677|Compound:st019204|FD-Score:4.61|P-value:1.93E-5 ID:SGTC_1713|Compound:st033146|FD-Score:3.80|P-value:3.55E-4 ID:SGTC_1723|Compound:st034307|FD-Score:4.49|P-value:3.14E-5 ID:SGTC_1759|Compound:st043059|FD-Score:3.74|P-value:4.31E-4 ID:SGTC_1816|Compound:st051957|FD-Score:4.55|P-value:2.49E-5 ID:SGTC_2002|Compound:4023503|FD-Score:-3.41|P-value:8.45E-4 ID:SGTC_2040|Compound:5100729|FD-Score:4.01|P-value:1.79E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-5.04|P-value:2.00E-6 ID:SGTC_2055|Compound:5235184|FD-Score:6.56|P-value:2.14E-9 ID:SGTC_2208|Compound:7283366|FD-Score:3.57|P-value:7.42E-4 ID:SGTC_2293|Compound:7624732|FD-Score:5.19|P-value:1.79E-6 ID:SGTC_2303|Compound:7774051|FD-Score:3.58|P-value:7.22E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:5.90|P-value:6.60E-8 ID:SGTC_2796|Compound:7949634|FD-Score:-4.70|P-value:8.38E-6 ID:SGTC_2822|Compound:7994718|FD-Score:3.71|P-value:4.86E-4 ID:SGTC_2826|Compound:7992541|FD-Score:4.55|P-value:2.44E-5 ID:SGTC_2903|Compound:5923464|FD-Score:5.05|P-value:3.16E-6 ID:SGTC_2992|Compound:9074591|FD-Score:3.50|P-value:9.25E-4 ID:SGTC_2995|Compound:9067767|FD-Score:-5.47|P-value:2.91E-7 ID:SGTC_2997|Compound:9067075|FD-Score:-4.09|P-value:8.78E-5 ID:SGTC_2999|Compound:9069765|FD-Score:-4.20|P-value:5.94E-5

Top fitness defect scores for YER123W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2055 5235184 6.56 200.0 µM 2.14E-9 ERG2 4 2.78E-11
2 SGTC_2779 methotrexate 5.90 400.0 µM 6.60E-8 12 1.87E-9
3 SGTC_1067 clotrimazole 5.54 1.4 µM 3.58E-7 azole & statin 15 1.49E-8
4 SGTC_737 1950-0117 5.52 57.9 µM 3.96E-7 3 1.69E-8
5 SGTC_2293 7624732 5.19 200.0 µM 1.79E-6 5 1.08E-7
6 SGTC_738 1315-0003 5.18 72.4 µM 1.83E-6 6 1.11E-7
7 SGTC_1174 0739-0097 5.16 51.1 µM 1.97E-6 redox potentiating 12 1.22E-7
8 SGTC_2903 5923464 5.05 63.9 µM 3.16E-6 2 2.17E-7
9 SGTC_439 amiodarone 4.98 34.4 µM 4.23E-6 NEO1-PIK1 67 3.10E-7
10 SGTC_1677 st019204 4.61 53.9 µM 1.93E-5 6 1.99E-6
11 SGTC_2826 7992541 4.55 19.5 µM 2.44E-5 10 2.67E-6
12 SGTC_1816 st051957 4.55 91.6 µM 2.49E-5 5 2.74E-6
13 SGTC_1723 st034307 4.49 2.5 µM 3.14E-5 mitochondrial processes 19 3.64E-6
14 SGTC_908 3448-7491 4.47 47.7 µM 3.40E-5 mitochondrial stress 94 4.00E-6
15 SGTC_245 methotrexate 4.45 289.6 µM 3.62E-5 31 4.33E-6
16 SGTC_1658 st012944 4.12 20.0 µM 1.21E-4 iron homeostasis 73 1.90E-5
17 SGTC_313 3474-0040 4.10 5.0 µM 1.30E-4 NEO1-PIK1 67 2.08E-5
18 SGTC_328 k915-0120 4.07 63.5 µM 1.43E-4 TSC3-RPN4 18 2.32E-5
19 SGTC_431 k089-0097 4.06 107.0 µM 1.49E-4 iron homeostasis 37 2.45E-5
20 SGTC_339 3091-4833 4.03 71.4 µM 1.65E-4 iron homeostasis 20 2.78E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.175 2.15E-24 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.149 4.05E-18 YGR267C FOL2 het GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
0.128 1.01E-13 YPL195W APL5 hom Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
0.127 1.62E-13 YOR074C CDC21 het Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
0.117 1.09E-11 YGR261C APL6 hom Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.102 3.18E-9 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
0.101 5.34E-9 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.097 2.03E-8 YOL052C SPE2 hom S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically
0.091 1.41E-7 YHR073W OSH3 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.088 3.58E-7 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.087 4.88E-7 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.086 6.11E-7 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.081 2.62E-6 YGL063W PUS2 hom Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification
0.080 3.22E-6 YGL102C_d YGL102C_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein
0.080 3.65E-6 YFR043C IRC6 hom Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci