YER125W / RSP5

E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER125W / RSP5

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:6.45|P-value:4.29E-10 ID:SGTC_2|Compound:0422-0095|FD-Score:3.26|P-value:8.80E-4 ID:SGTC_4|Compound:0960-0234|FD-Score:5.10|P-value:5.83E-7 ID:SGTC_42|Compound:0133-0086|FD-Score:3.44|P-value:4.78E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:3.46|P-value:4.49E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.86|P-value:1.77E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:4.45|P-value:1.08E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.49|P-value:4.01E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:3.90|P-value:9.34E-5 ID:SGTC_131|Compound:0141-0277|FD-Score:5.54|P-value:6.58E-8 ID:SGTC_143|Compound:4032-1194|FD-Score:5.10|P-value:5.74E-7 ID:SGTC_144|Compound:4161-2749|FD-Score:4.10|P-value:4.39E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:7.56|P-value:3.45E-13 ID:SGTC_266|Compound:formosanin C|FD-Score:5.22|P-value:3.28E-7 ID:SGTC_299|Compound:1273-0059|FD-Score:5.41|P-value:1.24E-7 ID:SGTC_393|Compound:acivicin|FD-Score:6.05|P-value:4.34E-9 ID:SGTC_426|Compound:0988-0037|FD-Score:3.35|P-value:6.57E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.56|P-value:5.19E-4 ID:SGTC_490|Compound:phenamil|FD-Score:3.27|P-value:8.39E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.15|P-value:3.62E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.39|P-value:8.85E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.43|P-value:7.87E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:10.00|P-value:1.33E-21 ID:SGTC_814|Compound:3342-0569|FD-Score:3.33|P-value:6.97E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:-3.50|P-value:6.35E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.49|P-value:4.09E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.24|P-value:9.18E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:4.22|P-value:2.73E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:5.78|P-value:1.83E-8 ID:SGTC_1081|Compound:sertraline|FD-Score:4.88|P-value:1.58E-6 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.33|P-value:6.88E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:4.43|P-value:1.15E-5 ID:SGTC_1293|Compound:1000-1278|FD-Score:-4.72|P-value:6.08E-6 ID:SGTC_1297|Compound:1068-0111|FD-Score:-4.00|P-value:1.10E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-5.22|P-value:6.22E-7 ID:SGTC_1313|Compound:1220-0047|FD-Score:-5.19|P-value:7.14E-7 ID:SGTC_1315|Compound:1237-0019|FD-Score:4.42|P-value:1.23E-5 ID:SGTC_1317|Compound:1273-0028|FD-Score:5.36|P-value:1.61E-7 ID:SGTC_1319|Compound:1306-0155|FD-Score:-3.77|P-value:2.54E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:4.57|P-value:6.35E-6 ID:SGTC_1407|Compound:3909-7909|FD-Score:3.65|P-value:2.37E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-4.13|P-value:6.65E-5 ID:SGTC_1673|Compound:st016611|FD-Score:-4.54|P-value:1.33E-5 ID:SGTC_1701|Compound:st028638|FD-Score:3.95|P-value:7.81E-5 ID:SGTC_1711|Compound:st032263|FD-Score:4.28|P-value:2.14E-5 ID:SGTC_1740|Compound:st038286|FD-Score:3.29|P-value:7.97E-4 ID:SGTC_1827|Compound:berberine|FD-Score:4.70|P-value:3.65E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:6.80|P-value:5.08E-11 ID:SGTC_1962|Compound:st076595|FD-Score:3.89|P-value:9.84E-5 ID:SGTC_2007|Compound:4011539|FD-Score:3.40|P-value:5.43E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-4.12|P-value:7.01E-5 ID:SGTC_2054|Compound:5233173|FD-Score:5.20|P-value:3.54E-7 ID:SGTC_2066|Compound:5222451|FD-Score:3.76|P-value:1.59E-4 ID:SGTC_2092|Compound:5303694|FD-Score:-3.37|P-value:9.51E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.81|P-value:1.33E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.78|P-value:1.49E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:3.25|P-value:8.85E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.00|P-value:6.48E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-5.47|P-value:1.90E-7 ID:SGTC_2257|Compound:7960413|FD-Score:5.05|P-value:7.36E-7 ID:SGTC_2302|Compound:7771753|FD-Score:3.97|P-value:7.13E-5 ID:SGTC_2322|Compound:7413319|FD-Score:6.00|P-value:5.58E-9 ID:SGTC_2413|Compound:inermin|FD-Score:4.99|P-value:9.52E-7 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.28|P-value:8.03E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.67|P-value:2.21E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.30|P-value:1.99E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:8.42|P-value:7.21E-16 ID:SGTC_2679|Compound:rapamycin|FD-Score:-4.35|P-value:2.87E-5 ID:SGTC_2734|Compound:clemastine|FD-Score:4.50|P-value:8.75E-6 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.60|P-value:4.45E-4 ID:SGTC_2792|Compound:7535235|FD-Score:4.65|P-value:4.53E-6 ID:SGTC_2841|Compound:9004761|FD-Score:-4.04|P-value:9.51E-5 ID:SGTC_2925|Compound:9010536|FD-Score:4.19|P-value:3.13E-5 ID:SGTC_2953|Compound:9074162|FD-Score:-3.40|P-value:8.61E-4 ID:SGTC_2981|Compound:9013952|FD-Score:3.50|P-value:3.98E-4 ID:SGTC_3062|Compound:9077494|FD-Score:4.17|P-value:3.31E-5 ID:SGTC_3126|Compound:9127922|FD-Score:5.80|P-value:1.72E-8 ID:SGTC_3143|Compound:9086658|FD-Score:-3.64|P-value:3.87E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-3.90|P-value:1.57E-4 ID:SGTC_3249|Compound:9135183|FD-Score:4.49|P-value:8.81E-6 ID:SGTC_3263|Compound:9137018|FD-Score:-5.27|P-value:4.95E-7 ID:SGTC_1|Compound:0258-0098|FD-Score:6.45|P-value:4.29E-10 ID:SGTC_2|Compound:0422-0095|FD-Score:3.26|P-value:8.80E-4 ID:SGTC_4|Compound:0960-0234|FD-Score:5.10|P-value:5.83E-7 ID:SGTC_42|Compound:0133-0086|FD-Score:3.44|P-value:4.78E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:3.46|P-value:4.49E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.86|P-value:1.77E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:4.45|P-value:1.08E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.49|P-value:4.01E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:3.90|P-value:9.34E-5 ID:SGTC_131|Compound:0141-0277|FD-Score:5.54|P-value:6.58E-8 ID:SGTC_143|Compound:4032-1194|FD-Score:5.10|P-value:5.74E-7 ID:SGTC_144|Compound:4161-2749|FD-Score:4.10|P-value:4.39E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:7.56|P-value:3.45E-13 ID:SGTC_266|Compound:formosanin C|FD-Score:5.22|P-value:3.28E-7 ID:SGTC_299|Compound:1273-0059|FD-Score:5.41|P-value:1.24E-7 ID:SGTC_393|Compound:acivicin|FD-Score:6.05|P-value:4.34E-9 ID:SGTC_426|Compound:0988-0037|FD-Score:3.35|P-value:6.57E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.56|P-value:5.19E-4 ID:SGTC_490|Compound:phenamil|FD-Score:3.27|P-value:8.39E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:4.15|P-value:3.62E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.39|P-value:8.85E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.43|P-value:7.87E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:10.00|P-value:1.33E-21 ID:SGTC_814|Compound:3342-0569|FD-Score:3.33|P-value:6.97E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:-3.50|P-value:6.35E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.49|P-value:4.09E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.24|P-value:9.18E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:4.22|P-value:2.73E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:5.78|P-value:1.83E-8 ID:SGTC_1081|Compound:sertraline|FD-Score:4.88|P-value:1.58E-6 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.33|P-value:6.88E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:4.43|P-value:1.15E-5 ID:SGTC_1293|Compound:1000-1278|FD-Score:-4.72|P-value:6.08E-6 ID:SGTC_1297|Compound:1068-0111|FD-Score:-4.00|P-value:1.10E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-5.22|P-value:6.22E-7 ID:SGTC_1313|Compound:1220-0047|FD-Score:-5.19|P-value:7.14E-7 ID:SGTC_1315|Compound:1237-0019|FD-Score:4.42|P-value:1.23E-5 ID:SGTC_1317|Compound:1273-0028|FD-Score:5.36|P-value:1.61E-7 ID:SGTC_1319|Compound:1306-0155|FD-Score:-3.77|P-value:2.54E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:4.57|P-value:6.35E-6 ID:SGTC_1407|Compound:3909-7909|FD-Score:3.65|P-value:2.37E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-4.13|P-value:6.65E-5 ID:SGTC_1673|Compound:st016611|FD-Score:-4.54|P-value:1.33E-5 ID:SGTC_1701|Compound:st028638|FD-Score:3.95|P-value:7.81E-5 ID:SGTC_1711|Compound:st032263|FD-Score:4.28|P-value:2.14E-5 ID:SGTC_1740|Compound:st038286|FD-Score:3.29|P-value:7.97E-4 ID:SGTC_1827|Compound:berberine|FD-Score:4.70|P-value:3.65E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:6.80|P-value:5.08E-11 ID:SGTC_1962|Compound:st076595|FD-Score:3.89|P-value:9.84E-5 ID:SGTC_2007|Compound:4011539|FD-Score:3.40|P-value:5.43E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-4.12|P-value:7.01E-5 ID:SGTC_2054|Compound:5233173|FD-Score:5.20|P-value:3.54E-7 ID:SGTC_2066|Compound:5222451|FD-Score:3.76|P-value:1.59E-4 ID:SGTC_2092|Compound:5303694|FD-Score:-3.37|P-value:9.51E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.81|P-value:1.33E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.78|P-value:1.49E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:3.25|P-value:8.85E-4 ID:SGTC_2150|Compound:5556613|FD-Score:4.00|P-value:6.48E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-5.47|P-value:1.90E-7 ID:SGTC_2257|Compound:7960413|FD-Score:5.05|P-value:7.36E-7 ID:SGTC_2302|Compound:7771753|FD-Score:3.97|P-value:7.13E-5 ID:SGTC_2322|Compound:7413319|FD-Score:6.00|P-value:5.58E-9 ID:SGTC_2413|Compound:inermin|FD-Score:4.99|P-value:9.52E-7 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.28|P-value:8.03E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.67|P-value:2.21E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.30|P-value:1.99E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:8.42|P-value:7.21E-16 ID:SGTC_2679|Compound:rapamycin|FD-Score:-4.35|P-value:2.87E-5 ID:SGTC_2734|Compound:clemastine|FD-Score:4.50|P-value:8.75E-6 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.60|P-value:4.45E-4 ID:SGTC_2792|Compound:7535235|FD-Score:4.65|P-value:4.53E-6 ID:SGTC_2841|Compound:9004761|FD-Score:-4.04|P-value:9.51E-5 ID:SGTC_2925|Compound:9010536|FD-Score:4.19|P-value:3.13E-5 ID:SGTC_2953|Compound:9074162|FD-Score:-3.40|P-value:8.61E-4 ID:SGTC_2981|Compound:9013952|FD-Score:3.50|P-value:3.98E-4 ID:SGTC_3062|Compound:9077494|FD-Score:4.17|P-value:3.31E-5 ID:SGTC_3126|Compound:9127922|FD-Score:5.80|P-value:1.72E-8 ID:SGTC_3143|Compound:9086658|FD-Score:-3.64|P-value:3.87E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-3.90|P-value:1.57E-4 ID:SGTC_3249|Compound:9135183|FD-Score:4.49|P-value:8.81E-6 ID:SGTC_3263|Compound:9137018|FD-Score:-5.27|P-value:4.95E-7

Top fitness defect scores for YER125W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_778 3456-2434 10.00 196.0 µM 1.33E-21 mitochondrial response to ROS 1 7.36E-24 2.12
2 SGTC_2619 obliquin 8.42 87.9 µM 7.21E-16 cell wall 5 1.96E-17 0.28
3 SGTC_145 3737-0113 7.56 38.4 µM 3.45E-13 DNA intercalators 6 1.95E-14 0.30
4 SGTC_1926 methyl fluorone black 6.80 53.6 µM 5.08E-11 DNA intercalators 8 5.14E-12 0.80
5 SGTC_1 0258-0098 6.45 57.4 µM 4.29E-10 heme biosynthesis & mitochondrial translocase 4 5.56E-11 0.24
6 SGTC_393 acivicin 6.05 6.2 µM 4.34E-9 3 7.35E-10 0.01
7 SGTC_2322 7413319 6.00 38.2 µM 5.58E-9 ERAD & cell cycle 11 9.71E-10 0.30
8 SGTC_3126 9127922 5.80 49.5 µM 1.72E-8 fatty acid desaturase (OLE1) 11 3.40E-9 0.25
9 SGTC_1044 1222-0021 5.78 1.8 µM 1.83E-8 heme biosynthesis & mitochondrial translocase 13 3.66E-9 0.02
10 SGTC_131 0141-0277 5.54 33.9 µM 6.58E-8 4 1.52E-8 0.83
11 SGTC_299 1273-0059 5.41 1.4 µM 1.24E-7 3 3.07E-8 0.05
12 SGTC_1317 1273-0028 5.36 910.0 nM 1.61E-7 azole & statin 8 4.12E-8 0.39
13 SGTC_266 formosanin C 5.22 406.0 nM 3.28E-7 sphingolipid biosynthesis & PDR1 12 9.06E-8 0.09
14 SGTC_2054 5233173 5.20 129.0 µM 3.54E-7 mitochondrial response to ROS 1 9.86E-8 1.91
15 SGTC_143 4032-1194 5.10 15.7 µM 5.74E-7 7 1.69E-7 0.18
16 SGTC_4 0960-0234 5.10 25.7 µM 5.83E-7 Golgi 5 1.71E-7 0.02
17 SGTC_2257 7960413 5.05 200.0 µM 7.36E-7 NEO1-PIK1 14 2.22E-7 0.48
18 SGTC_2413 inermin 4.99 26.1 µM 9.52E-7 10 2.96E-7 0.20
19 SGTC_1081 sertraline 4.88 6.0 µM 1.58E-6 60S ribosome export 3 5.21E-7 0.64
20 SGTC_70 0141-0289 4.86 43.3 µM 1.77E-6 mitochondrial stress 13 5.89E-7 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.218 3.36E-37 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.192 2.34E-29 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.177 4.96E-25 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.172 8.74E-24 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.167 2.60E-22 YGL002W ERP6 hom Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
0.154 2.45E-19 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.150 1.97E-18 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.148 5.44E-18 YNL124W NAF1 het RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.141 2.20E-16 YPL047W SGF11 hom Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
0.141 2.78E-16 YMR001C CDC5 het Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate
0.141 3.05E-16 YKR035C_d OPI8_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
0.132 1.75E-14 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.132 1.98E-14 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.127 1.80E-13 YGR070W ROM1 hom GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
0.126 2.46E-13 YJL204C RCY1 hom F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth