YER127W / LCP5

Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER127W / LCP5

Click on Significant Values for Screen Details ID:SGTC_116|Compound:0155-0182|FD-Score:-4.99|P-value:3.91E-7 ID:SGTC_372|Compound:0155-0182|FD-Score:-5.54|P-value:2.10E-8 ID:SGTC_625|Compound:k213-0058|FD-Score:7.12|P-value:5.52E-13 ID:SGTC_775|Compound:4577-0580|FD-Score:4.11|P-value:1.82E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-7.04|P-value:1.51E-12 ID:SGTC_785|Compound:1270-0111|FD-Score:4.10|P-value:1.98E-5 ID:SGTC_787|Compound:4152-0075|FD-Score:4.95|P-value:3.53E-7 ID:SGTC_800|Compound:3448-7362|FD-Score:5.32|P-value:4.96E-8 ID:SGTC_883|Compound:0850-0245|FD-Score:3.32|P-value:4.27E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:-3.30|P-value:5.66E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.40|P-value:3.91E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.49|P-value:2.25E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:3.29|P-value:4.71E-4 ID:SGTC_1197|Compound:1601-0112|FD-Score:6.28|P-value:1.62E-10 ID:SGTC_1210|Compound:3013-0140|FD-Score:6.41|P-value:7.04E-11 ID:SGTC_1297|Compound:1068-0111|FD-Score:-3.20|P-value:7.97E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:4.25|P-value:9.95E-6 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.48|P-value:2.33E-4 ID:SGTC_1694|Compound:st024011|FD-Score:3.51|P-value:2.11E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.46|P-value:2.50E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.17|P-value:8.78E-4 ID:SGTC_1781|Compound:st038725|FD-Score:4.88|P-value:5.04E-7 ID:SGTC_1871|Compound:st057448|FD-Score:3.96|P-value:3.52E-5 ID:SGTC_1914|Compound:st062367|FD-Score:4.66|P-value:1.49E-6 ID:SGTC_1917|Compound:st066507|FD-Score:-3.86|P-value:6.85E-5 ID:SGTC_1919|Compound:st067113|FD-Score:-3.87|P-value:6.52E-5 ID:SGTC_1970|Compound:st070122|FD-Score:3.68|P-value:1.12E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-6.65|P-value:2.23E-11 ID:SGTC_2191|Compound:5911152|FD-Score:3.88|P-value:4.96E-5 ID:SGTC_2195|Compound:6536160|FD-Score:6.27|P-value:1.76E-10 ID:SGTC_2451|Compound:5856223|FD-Score:-3.33|P-value:5.07E-4 ID:SGTC_2483|Compound:5729050|FD-Score:-6.07|P-value:9.09E-10 ID:SGTC_2484|Compound:5809818|FD-Score:-4.88|P-value:6.77E-7 ID:SGTC_2488|Compound:5268135|FD-Score:-4.49|P-value:4.52E-6 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.46|P-value:2.55E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.47|P-value:2.42E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.14|P-value:7.89E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:5.97|P-value:1.15E-9 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.10|P-value:9.19E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:4.85|P-value:5.75E-7 ID:SGTC_2649|Compound:anisomycin|FD-Score:3.23|P-value:5.86E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:-4.09|P-value:2.63E-5 ID:SGTC_2785|Compound:7756463|FD-Score:3.96|P-value:3.49E-5 ID:SGTC_2795|Compound:7780010|FD-Score:-3.22|P-value:7.59E-4 ID:SGTC_2803|Compound:7707263|FD-Score:3.15|P-value:7.78E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.57|P-value:1.68E-4 ID:SGTC_2888|Compound:9058547|FD-Score:-3.14|P-value:9.77E-4 ID:SGTC_2892|Compound:9047358|FD-Score:3.47|P-value:2.46E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.21|P-value:6.27E-4 ID:SGTC_2930|Compound:9013230|FD-Score:-3.61|P-value:1.81E-4 ID:SGTC_2941|Compound:9056145|FD-Score:-3.74|P-value:1.10E-4 ID:SGTC_2943|Compound:9068529|FD-Score:5.16|P-value:1.18E-7 ID:SGTC_2944|Compound:9067346|FD-Score:6.95|P-value:1.82E-12 ID:SGTC_2948|Compound:9070189|FD-Score:-5.72|P-value:7.30E-9 ID:SGTC_3013|Compound:9080133|FD-Score:4.23|P-value:1.08E-5 ID:SGTC_3192|Compound:9110362|FD-Score:6.08|P-value:5.92E-10 ID:SGTC_3196|Compound:9111891|FD-Score:4.12|P-value:1.78E-5 ID:SGTC_3307|Compound:9126885|FD-Score:-3.49|P-value:2.91E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-4.99|P-value:3.91E-7 ID:SGTC_372|Compound:0155-0182|FD-Score:-5.54|P-value:2.10E-8 ID:SGTC_625|Compound:k213-0058|FD-Score:7.12|P-value:5.52E-13 ID:SGTC_775|Compound:4577-0580|FD-Score:4.11|P-value:1.82E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-7.04|P-value:1.51E-12 ID:SGTC_785|Compound:1270-0111|FD-Score:4.10|P-value:1.98E-5 ID:SGTC_787|Compound:4152-0075|FD-Score:4.95|P-value:3.53E-7 ID:SGTC_800|Compound:3448-7362|FD-Score:5.32|P-value:4.96E-8 ID:SGTC_883|Compound:0850-0245|FD-Score:3.32|P-value:4.27E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:-3.30|P-value:5.66E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.40|P-value:3.91E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.49|P-value:2.25E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:3.29|P-value:4.71E-4 ID:SGTC_1197|Compound:1601-0112|FD-Score:6.28|P-value:1.62E-10 ID:SGTC_1210|Compound:3013-0140|FD-Score:6.41|P-value:7.04E-11 ID:SGTC_1297|Compound:1068-0111|FD-Score:-3.20|P-value:7.97E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:4.25|P-value:9.95E-6 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.48|P-value:2.33E-4 ID:SGTC_1694|Compound:st024011|FD-Score:3.51|P-value:2.11E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.46|P-value:2.50E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.17|P-value:8.78E-4 ID:SGTC_1781|Compound:st038725|FD-Score:4.88|P-value:5.04E-7 ID:SGTC_1871|Compound:st057448|FD-Score:3.96|P-value:3.52E-5 ID:SGTC_1914|Compound:st062367|FD-Score:4.66|P-value:1.49E-6 ID:SGTC_1917|Compound:st066507|FD-Score:-3.86|P-value:6.85E-5 ID:SGTC_1919|Compound:st067113|FD-Score:-3.87|P-value:6.52E-5 ID:SGTC_1970|Compound:st070122|FD-Score:3.68|P-value:1.12E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-6.65|P-value:2.23E-11 ID:SGTC_2191|Compound:5911152|FD-Score:3.88|P-value:4.96E-5 ID:SGTC_2195|Compound:6536160|FD-Score:6.27|P-value:1.76E-10 ID:SGTC_2451|Compound:5856223|FD-Score:-3.33|P-value:5.07E-4 ID:SGTC_2483|Compound:5729050|FD-Score:-6.07|P-value:9.09E-10 ID:SGTC_2484|Compound:5809818|FD-Score:-4.88|P-value:6.77E-7 ID:SGTC_2488|Compound:5268135|FD-Score:-4.49|P-value:4.52E-6 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.46|P-value:2.55E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.47|P-value:2.42E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.14|P-value:7.89E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:5.97|P-value:1.15E-9 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.10|P-value:9.19E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:4.85|P-value:5.75E-7 ID:SGTC_2649|Compound:anisomycin|FD-Score:3.23|P-value:5.86E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:-4.09|P-value:2.63E-5 ID:SGTC_2785|Compound:7756463|FD-Score:3.96|P-value:3.49E-5 ID:SGTC_2795|Compound:7780010|FD-Score:-3.22|P-value:7.59E-4 ID:SGTC_2803|Compound:7707263|FD-Score:3.15|P-value:7.78E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.57|P-value:1.68E-4 ID:SGTC_2888|Compound:9058547|FD-Score:-3.14|P-value:9.77E-4 ID:SGTC_2892|Compound:9047358|FD-Score:3.47|P-value:2.46E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.21|P-value:6.27E-4 ID:SGTC_2930|Compound:9013230|FD-Score:-3.61|P-value:1.81E-4 ID:SGTC_2941|Compound:9056145|FD-Score:-3.74|P-value:1.10E-4 ID:SGTC_2943|Compound:9068529|FD-Score:5.16|P-value:1.18E-7 ID:SGTC_2944|Compound:9067346|FD-Score:6.95|P-value:1.82E-12 ID:SGTC_2948|Compound:9070189|FD-Score:-5.72|P-value:7.30E-9 ID:SGTC_3013|Compound:9080133|FD-Score:4.23|P-value:1.08E-5 ID:SGTC_3192|Compound:9110362|FD-Score:6.08|P-value:5.92E-10 ID:SGTC_3196|Compound:9111891|FD-Score:4.12|P-value:1.78E-5 ID:SGTC_3307|Compound:9126885|FD-Score:-3.49|P-value:2.91E-4

Top fitness defect scores for YER127W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_625 k213-0058 7.12 121.0 µM 5.52E-13 translation 1 5.58E-13 0.80
2 SGTC_2944 9067346 6.95 25.6 µM 1.82E-12 2 1.85E-12 0.99
3 SGTC_1210 3013-0140 6.41 750.0 nM 7.04E-11 1 7.26E-11 2.25
4 SGTC_1197 1601-0112 6.28 267.0 µM 1.62E-10 1 1.67E-10 1.08
5 SGTC_2195 6536160 6.27 144.2 µM 1.76E-10 60S ribosome export 1 1.82E-10 2.37
6 SGTC_3192 9110362 6.08 49.5 µM 5.92E-10 mitochondrial processes 1 6.14E-10 1.11
7 SGTC_2629 bisabolol 5.97 100.0 µM 1.15E-9 3 1.20E-9 1.02
8 SGTC_800 3448-7362 5.32 155.0 µM 4.96E-8 translation 30 5.21E-8 0.23
9 SGTC_2943 9068529 5.16 45.3 µM 1.18E-7 6 1.25E-7 0.08
10 SGTC_787 4152-0075 4.95 138.0 µM 3.53E-7 1 3.72E-7 0.47
11 SGTC_1781 st038725 4.88 66.3 µM 5.04E-7 NEO1 7 5.33E-7 0.43
12 SGTC_2634 4'-methoxyflavone 4.85 100.0 µM 5.75E-7 3 6.07E-7 0.26
13 SGTC_1914 st062367 4.66 72.6 µM 1.49E-6 2 1.57E-6 0.40
14 SGTC_1298 1079-1278 4.25 263.0 µM 9.95E-6 DNA damage response 4 1.06E-5 0.29
15 SGTC_3013 9080133 4.23 71.4 µM 1.08E-5 2 1.15E-5 0.45
16 SGTC_3196 9111891 4.12 49.5 µM 1.78E-5 mitochondrial processes 2 1.89E-5 0.24
17 SGTC_775 4577-0580 4.11 57.3 µM 1.82E-5 3 1.94E-5 0.24
18 SGTC_785 1270-0111 4.10 65.3 µM 1.98E-5 5 2.10E-5 0.07
19 SGTC_2785 7756463 3.96 72.7 µM 3.49E-5 17 3.71E-5 0.00
20 SGTC_1871 st057448 3.96 67.3 µM 3.52E-5 8 3.75E-5 0.18

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.142 1.18E-16 YJL136C RPS21B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
0.138 9.39E-16 YOR078W BUD21 hom Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
0.134 6.62E-15 YOL121C RPS19A hom Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication
0.130 4.33E-14 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.125 3.42E-13 YJL069C UTP18 het Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
0.124 6.06E-13 YLR129W DIP2 het Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex
0.123 1.02E-12 YKL153W_d YKL153W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
0.121 2.38E-12 YIR021W-A_p YIR021W-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.111 1.01E-10 YHR164C DNA2 het Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
0.110 1.88E-10 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.108 3.88E-10 YBR236C ABD1 het Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
0.107 4.84E-10 YDR023W SES1 het Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
0.106 7.35E-10 YLR403W SFP1 hom Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion
0.105 1.03E-9 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.103 2.50E-9 YEL019C MMS21 het SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination