YER129W / SAK1

Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome

Zygosity: Homozygous strain
fixedexpanded
Profile for YER129W / SAK1

Click on Significant Values for Screen Details ID:SGTC_50|Compound:0929-0078|FD-Score:4.10|P-value:5.38E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:4.74|P-value:3.66E-6 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.85|P-value:1.29E-4 ID:SGTC_229|Compound:benomyl|FD-Score:3.44|P-value:5.68E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.34|P-value:2.03E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:4.16|P-value:4.24E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:3.45|P-value:5.54E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:-3.80|P-value:1.53E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:10.40|P-value:2.84E-23 ID:SGTC_393|Compound:acivicin|FD-Score:5.57|P-value:6.98E-8 ID:SGTC_486|Compound:niguldipine|FD-Score:3.53|P-value:4.31E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.29|P-value:9.26E-4 ID:SGTC_639|Compound:0833-0042|FD-Score:4.27|P-value:2.73E-5 ID:SGTC_701|Compound:4424-0219|FD-Score:-3.50|P-value:4.47E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:3.32|P-value:8.48E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-5.91|P-value:1.04E-8 ID:SGTC_944|Compound:3370-0224|FD-Score:7.05|P-value:1.28E-11 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.05|P-value:6.34E-5 ID:SGTC_1109|Compound:tnp00067|FD-Score:4.18|P-value:3.80E-5 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.64|P-value:2.95E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.70|P-value:2.32E-4 ID:SGTC_1277|Compound:0873-0036|FD-Score:5.77|P-value:2.35E-8 ID:SGTC_1280|Compound:0880-0253|FD-Score:3.30|P-value:8.95E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.73|P-value:3.91E-6 ID:SGTC_1302|Compound:1159-0335|FD-Score:-4.00|P-value:7.26E-5 ID:SGTC_1314|Compound:1222-0010|FD-Score:-3.36|P-value:7.11E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:5.00|P-value:1.11E-6 ID:SGTC_1521|Compound:st012942|FD-Score:3.51|P-value:4.49E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.03|P-value:6.97E-5 ID:SGTC_1618|Compound:st003135|FD-Score:3.42|P-value:6.11E-4 ID:SGTC_1653|Compound:st012112|FD-Score:3.76|P-value:1.90E-4 ID:SGTC_1683|Compound:st010134|FD-Score:5.36|P-value:1.98E-7 ID:SGTC_1859|Compound:st055998|FD-Score:-3.49|P-value:4.67E-4 ID:SGTC_1886|Compound:st057356|FD-Score:6.42|P-value:6.27E-10 ID:SGTC_1910|Compound:st062352|FD-Score:4.66|P-value:5.18E-6 ID:SGTC_2013|Compound:4024628|FD-Score:3.76|P-value:1.87E-4 ID:SGTC_2047|Compound:4141938|FD-Score:4.13|P-value:4.63E-5 ID:SGTC_2083|Compound:5306046|FD-Score:3.87|P-value:1.26E-4 ID:SGTC_2111|Compound:5486399|FD-Score:4.68|P-value:4.83E-6 ID:SGTC_2115|Compound:tiliquinol|FD-Score:3.58|P-value:3.61E-4 ID:SGTC_2123|Compound:5259487|FD-Score:-3.78|P-value:1.66E-4 ID:SGTC_2384|Compound:9036245|FD-Score:3.27|P-value:9.90E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.31|P-value:8.92E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:4.75|P-value:3.56E-6 ID:SGTC_2544|Compound:sphondin|FD-Score:6.93|P-value:2.68E-11 ID:SGTC_2545|Compound:solidagenone|FD-Score:-3.34|P-value:7.58E-4 ID:SGTC_2556|Compound:citropten|FD-Score:3.73|P-value:2.12E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:5.99|P-value:7.46E-9 ID:SGTC_2875|Compound:9039966|FD-Score:4.84|P-value:2.34E-6 ID:SGTC_3021|Compound:9087658|FD-Score:-3.86|P-value:1.22E-4 ID:SGTC_3029|Compound:9088683|FD-Score:3.51|P-value:4.52E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.99|P-value:7.99E-5 ID:SGTC_50|Compound:0929-0078|FD-Score:4.10|P-value:5.38E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:4.74|P-value:3.66E-6 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.85|P-value:1.29E-4 ID:SGTC_229|Compound:benomyl|FD-Score:3.44|P-value:5.68E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.34|P-value:2.03E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:4.16|P-value:4.24E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:3.45|P-value:5.54E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:-3.80|P-value:1.53E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:10.40|P-value:2.84E-23 ID:SGTC_393|Compound:acivicin|FD-Score:5.57|P-value:6.98E-8 ID:SGTC_486|Compound:niguldipine|FD-Score:3.53|P-value:4.31E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:3.29|P-value:9.26E-4 ID:SGTC_639|Compound:0833-0042|FD-Score:4.27|P-value:2.73E-5 ID:SGTC_701|Compound:4424-0219|FD-Score:-3.50|P-value:4.47E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:3.32|P-value:8.48E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-5.91|P-value:1.04E-8 ID:SGTC_944|Compound:3370-0224|FD-Score:7.05|P-value:1.28E-11 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.05|P-value:6.34E-5 ID:SGTC_1109|Compound:tnp00067|FD-Score:4.18|P-value:3.80E-5 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.64|P-value:2.95E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.70|P-value:2.32E-4 ID:SGTC_1277|Compound:0873-0036|FD-Score:5.77|P-value:2.35E-8 ID:SGTC_1280|Compound:0880-0253|FD-Score:3.30|P-value:8.95E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.73|P-value:3.91E-6 ID:SGTC_1302|Compound:1159-0335|FD-Score:-4.00|P-value:7.26E-5 ID:SGTC_1314|Compound:1222-0010|FD-Score:-3.36|P-value:7.11E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:5.00|P-value:1.11E-6 ID:SGTC_1521|Compound:st012942|FD-Score:3.51|P-value:4.49E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.03|P-value:6.97E-5 ID:SGTC_1618|Compound:st003135|FD-Score:3.42|P-value:6.11E-4 ID:SGTC_1653|Compound:st012112|FD-Score:3.76|P-value:1.90E-4 ID:SGTC_1683|Compound:st010134|FD-Score:5.36|P-value:1.98E-7 ID:SGTC_1859|Compound:st055998|FD-Score:-3.49|P-value:4.67E-4 ID:SGTC_1886|Compound:st057356|FD-Score:6.42|P-value:6.27E-10 ID:SGTC_1910|Compound:st062352|FD-Score:4.66|P-value:5.18E-6 ID:SGTC_2013|Compound:4024628|FD-Score:3.76|P-value:1.87E-4 ID:SGTC_2047|Compound:4141938|FD-Score:4.13|P-value:4.63E-5 ID:SGTC_2083|Compound:5306046|FD-Score:3.87|P-value:1.26E-4 ID:SGTC_2111|Compound:5486399|FD-Score:4.68|P-value:4.83E-6 ID:SGTC_2115|Compound:tiliquinol|FD-Score:3.58|P-value:3.61E-4 ID:SGTC_2123|Compound:5259487|FD-Score:-3.78|P-value:1.66E-4 ID:SGTC_2384|Compound:9036245|FD-Score:3.27|P-value:9.90E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.31|P-value:8.92E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:4.75|P-value:3.56E-6 ID:SGTC_2544|Compound:sphondin|FD-Score:6.93|P-value:2.68E-11 ID:SGTC_2545|Compound:solidagenone|FD-Score:-3.34|P-value:7.58E-4 ID:SGTC_2556|Compound:citropten|FD-Score:3.73|P-value:2.12E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:5.99|P-value:7.46E-9 ID:SGTC_2875|Compound:9039966|FD-Score:4.84|P-value:2.34E-6 ID:SGTC_3021|Compound:9087658|FD-Score:-3.86|P-value:1.22E-4 ID:SGTC_3029|Compound:9088683|FD-Score:3.51|P-value:4.52E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.99|P-value:7.99E-5

Top fitness defect scores for YER129W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_387 norethindrone acetate 10.40 66.7 µM 2.84E-23 1 9.48E-26
2 SGTC_944 3370-0224 7.05 231.0 µM 1.28E-11 3 9.05E-13
3 SGTC_2544 sphondin 6.93 68.5 µM 2.68E-11 RNA pol III & RNase P/MRP 4 2.05E-12
4 SGTC_1886 st057356 6.42 49.9 µM 6.27E-10 60S ribosome export 3 6.87E-11
5 SGTC_2584 androsterone 5.99 48.7 µM 7.46E-9 cell wall 60 1.08E-9
6 SGTC_1277 0873-0036 5.77 22.0 µM 2.35E-8 PDR1 8 3.86E-9
7 SGTC_393 acivicin 5.57 6.2 µM 6.98E-8 8 1.30E-8
8 SGTC_1683 st010134 5.36 710.0 nM 1.98E-7 8 4.13E-8
9 SGTC_1325 1431-0015 5.00 227.0 µM 1.11E-6 Golgi 5 2.82E-7
10 SGTC_2875 9039966 4.84 71.4 µM 2.34E-6 4 6.45E-7
11 SGTC_2531 methyl deoxycholate 4.75 70.1 µM 3.56E-6 13 1.03E-6
12 SGTC_58 0957-0393 4.74 9.0 µM 3.66E-6 cell wall 312 1.06E-6
13 SGTC_1290 0986-0249 4.73 10.2 µM 3.91E-6 DNA intercalators 10 1.14E-6
14 SGTC_2111 5486399 4.68 200.0 µM 4.83E-6 24 1.44E-6
15 SGTC_1910 st062352 4.66 60.4 µM 5.18E-6 16 1.56E-6
16 SGTC_238 5hr heat shock (37°C) + nsc-638432 4.34 8.9 µM 2.03E-5 heat shock/prefoldin 31 7.10E-6
17 SGTC_639 0833-0042 4.27 114.0 µM 2.73E-5 36 9.87E-6
18 SGTC_1109 tnp00067 4.18 928.3 nM 3.80E-5 11 1.43E-5
19 SGTC_266 formosanin C 4.16 406.0 nM 4.24E-5 sphingolipid biosynthesis & PDR1 62 1.61E-5
20 SGTC_2047 4141938 4.13 200.0 µM 4.63E-5 6 1.78E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.152 8.02E-19 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.138 8.65E-16 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.134 8.07E-15 YBR134W_d YBR134W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.133 8.63E-15 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.133 1.09E-14 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.131 2.39E-14 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.112 8.78E-11 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.107 4.52E-10 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.103 2.39E-9 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.103 2.48E-9 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.102 3.45E-9 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.100 6.35E-9 YBR244W GPX2 hom Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
0.099 9.17E-9 YJL020C BBC1 hom Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches
0.097 2.16E-8 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.096 2.90E-8 YML119W_p YML119W_p hom Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate