YER140W / EMP65

ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Zygosity: Homozygous strain
fixedexpanded
Profile for YER140W / EMP65

Click on Significant Values for Screen Details

Top fitness defect scores for YER140W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_444 curcumin 7.23 90.4 µM 3.94E-14 iron homeostasis 3 2.45E-13
2 SGTC_2050 5263056 5.26 171.0 µM 2.65E-8 fatty acid desaturase (OLE1) 21 7.10E-8
3 SGTC_2751 anetholtrithion 4.66 17.2 µM 7.34E-7 14 1.60E-6
4 SGTC_1178 0988-0033 4.47 191.0 µM 1.87E-6 19 3.85E-6
5 SGTC_939 1165-0479 4.44 45.3 µM 2.25E-6 15 4.57E-6
6 SGTC_1585 2',4'-dihydroxychalcone 4.09 10.4 µM 1.20E-5 29 2.20E-5
7 SGTC_2375 9072683 4.03 200.0 µM 1.55E-5 mitochondrial processes 18 2.80E-5
8 SGTC_2825 7998094 3.82 58.4 µM 3.84E-5 fatty acid desaturase (OLE1) 35 6.55E-5
9 SGTC_2563 tomatine 3.79 8.5 µM 4.48E-5 54 7.58E-5
10 SGTC_2606 farnesol 3.66 9.8 µM 7.74E-5 27 1.27E-4
11 SGTC_2067 5224629 3.65 34.1 µM 8.18E-5 31 1.34E-4
12 SGTC_2056 5235370 3.53 200.0 µM 1.32E-4 59 2.10E-4
13 SGTC_1830 st055982 3.52 50.3 µM 1.36E-4 34 2.15E-4
14 SGTC_2527 2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone 3.44 63.6 µM 1.90E-4 63 2.95E-4
15 SGTC_2151 5563267 3.41 85.4 µM 2.12E-4 37 3.26E-4
16 SGTC_2247 cerulenin 3.28 830.0 nM 3.51E-4 43 5.24E-4
17 SGTC_1584 6-fluoro-dl-tryptophan 3.20 90.0 µM 4.64E-4 mitochondrial processes 43 6.81E-4
18 SGTC_1557 sumaresinolic acid 3.20 42.3 µM 4.72E-4 47 6.93E-4
19 SGTC_905 2-aminofluorene 3.20 215.0 µM 4.74E-4 cell wall signaling 58 6.95E-4
20 SGTC_3061 glucosamine 3.19 200.0 µM 4.81E-4 30 7.06E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.110 1.93E-10 YNL291C MID1 hom N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer
0.109 2.72E-10 YDR331W GPI8 het ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
0.099 8.06E-9 YPR176C BET2 het Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
0.099 8.36E-9 YOR284W HUA2 hom Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
0.095 3.90E-8 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.094 4.15E-8 YGL055W OLE1 het Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
0.091 1.15E-7 YPR085C ASA1 het Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
0.086 5.34E-7 YML105C SEC65 het Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
0.086 6.60E-7 YDR271C_d YDR271C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W
0.085 7.80E-7 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.084 9.99E-7 YOR304W ISW2 hom ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth
0.083 1.48E-6 YIL134C-A_p YIL134C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.082 2.17E-6 YER036C ARB1 het ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p
0.081 2.68E-6 YJL161W_p FMP33_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.080 3.74E-6 YGR087C PDC6 hom Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation