YER142C / MAG1

3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YER142C / MAG1

Click on Significant Values for Screen Details ID:SGTC_248|Compound:streptozotocin|FD-Score:8.44|P-value:2.60E-20 ID:SGTC_384|Compound:k007-0144|FD-Score:3.25|P-value:1.98E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:-3.11|P-value:3.97E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:-3.70|P-value:3.31E-5 ID:SGTC_739|Compound:1486-1183|FD-Score:3.63|P-value:3.93E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:-3.02|P-value:5.79E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:4.10|P-value:4.10E-6 ID:SGTC_873|Compound:0591-4978|FD-Score:4.02|P-value:6.20E-6 ID:SGTC_877|Compound:0771-0151|FD-Score:3.31|P-value:1.58E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:6.26|P-value:5.22E-12 ID:SGTC_919|Compound:tnp00227|FD-Score:3.81|P-value:1.66E-5 ID:SGTC_927|Compound:2425-1185|FD-Score:-2.87|P-value:9.94E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:5.00|P-value:2.78E-8 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.05|P-value:4.37E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.43|P-value:9.17E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:5.73|P-value:2.44E-10 ID:SGTC_1516|Compound:st013856|FD-Score:4.08|P-value:4.55E-6 ID:SGTC_1568|Compound:digitonin|FD-Score:-3.04|P-value:5.22E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:2.93|P-value:7.13E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.29|P-value:1.72E-4 ID:SGTC_1755|Compound:st041960|FD-Score:2.85|P-value:9.29E-4 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:3.18|P-value:2.69E-4 ID:SGTC_2073|Compound:5212005|FD-Score:3.27|P-value:1.84E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.36|P-value:1.25E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.33|P-value:1.41E-4 ID:SGTC_2172|Compound:5754775|FD-Score:2.91|P-value:7.45E-4 ID:SGTC_2236|Compound:dapsone|FD-Score:3.62|P-value:4.14E-5 ID:SGTC_2238|Compound:6671620|FD-Score:4.35|P-value:1.11E-6 ID:SGTC_2254|Compound:7938975|FD-Score:3.13|P-value:3.25E-4 ID:SGTC_2258|Compound:7960471|FD-Score:-3.04|P-value:5.31E-4 ID:SGTC_2273|Compound:7815444|FD-Score:-3.23|P-value:2.50E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.47|P-value:7.98E-5 ID:SGTC_2310|Compound:7668677|FD-Score:-3.17|P-value:3.16E-4 ID:SGTC_2335|Compound:9018011|FD-Score:3.48|P-value:7.63E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:2.88|P-value:8.49E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.40|P-value:1.08E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-3.05|P-value:5.08E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.43|P-value:9.51E-5 ID:SGTC_2767|Compound:sibutramine|FD-Score:-2.93|P-value:8.12E-4 ID:SGTC_2815|Compound:7968870|FD-Score:3.15|P-value:3.02E-4 ID:SGTC_2818|Compound:7996756|FD-Score:3.36|P-value:1.26E-4 ID:SGTC_2836|Compound:9010338|FD-Score:2.90|P-value:7.74E-4 ID:SGTC_2842|Compound:9000675|FD-Score:-3.09|P-value:4.40E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.50|P-value:6.82E-5 ID:SGTC_3007|Compound:9078383|FD-Score:-3.11|P-value:4.08E-4 ID:SGTC_3087|Compound:9117406|FD-Score:4.24|P-value:1.96E-6 ID:SGTC_3181|Compound:9108277|FD-Score:4.01|P-value:6.38E-6 ID:SGTC_248|Compound:streptozotocin|FD-Score:8.44|P-value:2.60E-20 ID:SGTC_384|Compound:k007-0144|FD-Score:3.25|P-value:1.98E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:-3.11|P-value:3.97E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:-3.70|P-value:3.31E-5 ID:SGTC_739|Compound:1486-1183|FD-Score:3.63|P-value:3.93E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:-3.02|P-value:5.79E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:4.10|P-value:4.10E-6 ID:SGTC_873|Compound:0591-4978|FD-Score:4.02|P-value:6.20E-6 ID:SGTC_877|Compound:0771-0151|FD-Score:3.31|P-value:1.58E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:6.26|P-value:5.22E-12 ID:SGTC_919|Compound:tnp00227|FD-Score:3.81|P-value:1.66E-5 ID:SGTC_927|Compound:2425-1185|FD-Score:-2.87|P-value:9.94E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:5.00|P-value:2.78E-8 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.05|P-value:4.37E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.43|P-value:9.17E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:5.73|P-value:2.44E-10 ID:SGTC_1516|Compound:st013856|FD-Score:4.08|P-value:4.55E-6 ID:SGTC_1568|Compound:digitonin|FD-Score:-3.04|P-value:5.22E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:2.93|P-value:7.13E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.29|P-value:1.72E-4 ID:SGTC_1755|Compound:st041960|FD-Score:2.85|P-value:9.29E-4 ID:SGTC_1929|Compound:genistein trimethyl ether|FD-Score:3.18|P-value:2.69E-4 ID:SGTC_2073|Compound:5212005|FD-Score:3.27|P-value:1.84E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.36|P-value:1.25E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.33|P-value:1.41E-4 ID:SGTC_2172|Compound:5754775|FD-Score:2.91|P-value:7.45E-4 ID:SGTC_2236|Compound:dapsone|FD-Score:3.62|P-value:4.14E-5 ID:SGTC_2238|Compound:6671620|FD-Score:4.35|P-value:1.11E-6 ID:SGTC_2254|Compound:7938975|FD-Score:3.13|P-value:3.25E-4 ID:SGTC_2258|Compound:7960471|FD-Score:-3.04|P-value:5.31E-4 ID:SGTC_2273|Compound:7815444|FD-Score:-3.23|P-value:2.50E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.47|P-value:7.98E-5 ID:SGTC_2310|Compound:7668677|FD-Score:-3.17|P-value:3.16E-4 ID:SGTC_2335|Compound:9018011|FD-Score:3.48|P-value:7.63E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:2.88|P-value:8.49E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.40|P-value:1.08E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-3.05|P-value:5.08E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.43|P-value:9.51E-5 ID:SGTC_2767|Compound:sibutramine|FD-Score:-2.93|P-value:8.12E-4 ID:SGTC_2815|Compound:7968870|FD-Score:3.15|P-value:3.02E-4 ID:SGTC_2818|Compound:7996756|FD-Score:3.36|P-value:1.26E-4 ID:SGTC_2836|Compound:9010338|FD-Score:2.90|P-value:7.74E-4 ID:SGTC_2842|Compound:9000675|FD-Score:-3.09|P-value:4.40E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.50|P-value:6.82E-5 ID:SGTC_3007|Compound:9078383|FD-Score:-3.11|P-value:4.08E-4 ID:SGTC_3087|Compound:9117406|FD-Score:4.24|P-value:1.96E-6 ID:SGTC_3181|Compound:9108277|FD-Score:4.01|P-value:6.38E-6

Top fitness defect scores for YER142C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_248 streptozotocin 8.44 5.2 mM 2.60E-20 DNA damage response 5 1.64E-17
2 SGTC_915 methyl methanesulfonate (MMS) 6.26 110.0 mM 5.22E-12 DNA damage response 9 1.94E-10
3 SGTC_1108 betulinic acid 5.73 729.1 nM 2.44E-10 1 5.15E-9
4 SGTC_996 3970-0799 5.00 1.8 µM 2.78E-8 54 2.93E-7
5 SGTC_2238 6671620 4.35 73.2 µM 1.11E-6 19 6.82E-6
6 SGTC_3087 9117406 4.24 49.5 µM 1.96E-6 15 1.11E-5
7 SGTC_856 0433-0016 4.10 11.3 µM 4.10E-6 13 2.08E-5
8 SGTC_1516 st013856 4.08 110.0 µM 4.55E-6 13 2.27E-5
9 SGTC_873 0591-4978 4.02 488.0 µM 6.20E-6 19 2.96E-5
10 SGTC_3181 9108277 4.01 49.5 µM 6.38E-6 iron homeostasis 13 3.03E-5
11 SGTC_919 tnp00227 3.81 403.6 nM 1.66E-5 RNA pol III & RNase P/MRP 39 6.86E-5
12 SGTC_739 1486-1183 3.63 203.0 µM 3.93E-5 24 1.44E-4
13 SGTC_2236 dapsone 3.62 200.0 µM 4.14E-5 16 1.50E-4
14 SGTC_2851 9015922 3.50 71.4 µM 6.82E-5 59 2.30E-4
15 SGTC_2335 9018011 3.48 154.2 µM 7.63E-5 42 2.53E-4
16 SGTC_2308 7584454 3.47 197.6 µM 7.98E-5 39 2.63E-4
17 SGTC_1104 tacrolimus 3.43 29.8 µM 9.17E-5 47 2.96E-4
18 SGTC_2634 4'-methoxyflavone 3.43 100.0 µM 9.51E-5 52 3.06E-4
19 SGTC_2553 4-methylesculetin 3.40 85.1 µM 1.08E-4 43 3.41E-4
20 SGTC_2128 5255449 3.36 168.5 µM 1.25E-4 59 3.87E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.188 4.63E-28 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.168 1.05E-22 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.161 6.59E-21 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.149 3.25E-18 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.134 7.33E-15 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.133 8.86E-15 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.129 6.94E-14 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.129 7.36E-14 YJR014W TMA22 hom Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress
0.121 1.84E-12 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.121 2.12E-12 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.119 4.80E-12 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.114 3.92E-11 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.110 1.65E-10 YHR109W CTM1 hom Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth
0.106 8.40E-10 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.105 1.27E-9 YJL087C TRL1 het tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes