YER148W / SPT15

TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER148W / SPT15

Click on Significant Values for Screen Details ID:SGTC_187|Compound:1488-2104|FD-Score:3.53|P-value:5.01E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:3.38|P-value:8.27E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:4.92|P-value:2.19E-6 ID:SGTC_374|Compound:0880-0231|FD-Score:-4.01|P-value:7.43E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.66|P-value:4.57E-8 ID:SGTC_525|Compound:k781-2675|FD-Score:6.60|P-value:3.52E-10 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.44|P-value:5.72E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:5.38|P-value:2.57E-7 ID:SGTC_754|Compound:3474-0146|FD-Score:-4.45|P-value:1.34E-5 ID:SGTC_757|Compound:1066-0008|FD-Score:-4.56|P-value:8.38E-6 ID:SGTC_837|Compound:0091-0265|FD-Score:3.50|P-value:5.51E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:3.34|P-value:9.27E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-4.09|P-value:5.65E-5 ID:SGTC_946|Compound:3434-1334|FD-Score:3.38|P-value:8.19E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.84|P-value:1.72E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:-4.30|P-value:2.39E-5 ID:SGTC_1219|Compound:1469-0211|FD-Score:4.30|P-value:3.03E-5 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.41|P-value:7.47E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-5.40|P-value:1.73E-7 ID:SGTC_1245|Compound:0418-0076|FD-Score:5.17|P-value:7.00E-7 ID:SGTC_1251|Compound:0490-5699|FD-Score:5.35|P-value:2.93E-7 ID:SGTC_1253|Compound:0550-0034|FD-Score:3.42|P-value:7.29E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:4.27|P-value:3.45E-5 ID:SGTC_1330|Compound:1469-0054|FD-Score:-3.67|P-value:2.56E-4 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.57|P-value:4.38E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:4.85|P-value:2.99E-6 ID:SGTC_1413|Compound:3910-0535|FD-Score:-3.74|P-value:2.04E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:4.91|P-value:2.29E-6 ID:SGTC_1416|Compound:3966-0321|FD-Score:5.59|P-value:8.99E-8 ID:SGTC_1462|Compound:k081-0014|FD-Score:4.52|P-value:1.23E-5 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:-3.33|P-value:8.23E-4 ID:SGTC_1623|Compound:st003709|FD-Score:4.75|P-value:4.72E-6 ID:SGTC_1646|Compound:st011709|FD-Score:3.34|P-value:9.34E-4 ID:SGTC_1653|Compound:st012112|FD-Score:3.51|P-value:5.35E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.08|P-value:7.07E-5 ID:SGTC_1794|Compound:st048918|FD-Score:-3.37|P-value:7.20E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.49|P-value:5.75E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.27|P-value:3.40E-5 ID:SGTC_1959|Compound:st077091|FD-Score:5.46|P-value:1.68E-7 ID:SGTC_2061|Compound:5245884|FD-Score:3.41|P-value:7.51E-4 ID:SGTC_2132|Compound:5317594|FD-Score:-3.65|P-value:2.82E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-3.42|P-value:6.07E-4 ID:SGTC_2163|Compound:5671233|FD-Score:3.58|P-value:4.31E-4 ID:SGTC_2295|Compound:7971645|FD-Score:-3.51|P-value:4.51E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.48|P-value:5.92E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-5.69|P-value:4.03E-8 ID:SGTC_2495|Compound:cafestol|FD-Score:-4.97|P-value:1.35E-6 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:5.45|P-value:1.78E-7 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.63|P-value:3.62E-4 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:-3.71|P-value:2.27E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-3.51|P-value:4.48E-4 ID:SGTC_2710|Compound:st078562|FD-Score:3.68|P-value:3.04E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.79|P-value:2.07E-4 ID:SGTC_2798|Compound:7813637|FD-Score:3.44|P-value:6.73E-4 ID:SGTC_2810|Compound:7959871|FD-Score:5.32|P-value:3.40E-7 ID:SGTC_2977|Compound:9011197|FD-Score:3.60|P-value:4.04E-4 ID:SGTC_2986|Compound:9022445|FD-Score:6.19|P-value:3.62E-9 ID:SGTC_3044|Compound:9093193|FD-Score:4.01|P-value:9.37E-5 ID:SGTC_3108|Compound:9121785|FD-Score:4.07|P-value:7.54E-5 ID:SGTC_3119|Compound:9125183|FD-Score:3.98|P-value:1.03E-4 ID:SGTC_3202|Compound:9113761|FD-Score:7.69|P-value:3.24E-13 ID:SGTC_3205|Compound:9113483|FD-Score:3.44|P-value:6.74E-4 ID:SGTC_3207|Compound:9114163|FD-Score:4.18|P-value:4.87E-5 ID:SGTC_3304|Compound:9125678|FD-Score:3.37|P-value:8.50E-4 ID:SGTC_187|Compound:1488-2104|FD-Score:3.53|P-value:5.01E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:3.38|P-value:8.27E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:4.92|P-value:2.19E-6 ID:SGTC_374|Compound:0880-0231|FD-Score:-4.01|P-value:7.43E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.66|P-value:4.57E-8 ID:SGTC_525|Compound:k781-2675|FD-Score:6.60|P-value:3.52E-10 ID:SGTC_568|Compound:1193-0097|FD-Score:-3.44|P-value:5.72E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:5.38|P-value:2.57E-7 ID:SGTC_754|Compound:3474-0146|FD-Score:-4.45|P-value:1.34E-5 ID:SGTC_757|Compound:1066-0008|FD-Score:-4.56|P-value:8.38E-6 ID:SGTC_837|Compound:0091-0265|FD-Score:3.50|P-value:5.51E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:3.34|P-value:9.27E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-4.09|P-value:5.65E-5 ID:SGTC_946|Compound:3434-1334|FD-Score:3.38|P-value:8.19E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.84|P-value:1.72E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:-4.30|P-value:2.39E-5 ID:SGTC_1219|Compound:1469-0211|FD-Score:4.30|P-value:3.03E-5 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.41|P-value:7.47E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-5.40|P-value:1.73E-7 ID:SGTC_1245|Compound:0418-0076|FD-Score:5.17|P-value:7.00E-7 ID:SGTC_1251|Compound:0490-5699|FD-Score:5.35|P-value:2.93E-7 ID:SGTC_1253|Compound:0550-0034|FD-Score:3.42|P-value:7.29E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:4.27|P-value:3.45E-5 ID:SGTC_1330|Compound:1469-0054|FD-Score:-3.67|P-value:2.56E-4 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.57|P-value:4.38E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:4.85|P-value:2.99E-6 ID:SGTC_1413|Compound:3910-0535|FD-Score:-3.74|P-value:2.04E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:4.91|P-value:2.29E-6 ID:SGTC_1416|Compound:3966-0321|FD-Score:5.59|P-value:8.99E-8 ID:SGTC_1462|Compound:k081-0014|FD-Score:4.52|P-value:1.23E-5 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:-3.33|P-value:8.23E-4 ID:SGTC_1623|Compound:st003709|FD-Score:4.75|P-value:4.72E-6 ID:SGTC_1646|Compound:st011709|FD-Score:3.34|P-value:9.34E-4 ID:SGTC_1653|Compound:st012112|FD-Score:3.51|P-value:5.35E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.08|P-value:7.07E-5 ID:SGTC_1794|Compound:st048918|FD-Score:-3.37|P-value:7.20E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.49|P-value:5.75E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.27|P-value:3.40E-5 ID:SGTC_1959|Compound:st077091|FD-Score:5.46|P-value:1.68E-7 ID:SGTC_2061|Compound:5245884|FD-Score:3.41|P-value:7.51E-4 ID:SGTC_2132|Compound:5317594|FD-Score:-3.65|P-value:2.82E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-3.42|P-value:6.07E-4 ID:SGTC_2163|Compound:5671233|FD-Score:3.58|P-value:4.31E-4 ID:SGTC_2295|Compound:7971645|FD-Score:-3.51|P-value:4.51E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.48|P-value:5.92E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-5.69|P-value:4.03E-8 ID:SGTC_2495|Compound:cafestol|FD-Score:-4.97|P-value:1.35E-6 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:5.45|P-value:1.78E-7 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.63|P-value:3.62E-4 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:-3.71|P-value:2.27E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-3.51|P-value:4.48E-4 ID:SGTC_2710|Compound:st078562|FD-Score:3.68|P-value:3.04E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.79|P-value:2.07E-4 ID:SGTC_2798|Compound:7813637|FD-Score:3.44|P-value:6.73E-4 ID:SGTC_2810|Compound:7959871|FD-Score:5.32|P-value:3.40E-7 ID:SGTC_2977|Compound:9011197|FD-Score:3.60|P-value:4.04E-4 ID:SGTC_2986|Compound:9022445|FD-Score:6.19|P-value:3.62E-9 ID:SGTC_3044|Compound:9093193|FD-Score:4.01|P-value:9.37E-5 ID:SGTC_3108|Compound:9121785|FD-Score:4.07|P-value:7.54E-5 ID:SGTC_3119|Compound:9125183|FD-Score:3.98|P-value:1.03E-4 ID:SGTC_3202|Compound:9113761|FD-Score:7.69|P-value:3.24E-13 ID:SGTC_3205|Compound:9113483|FD-Score:3.44|P-value:6.74E-4 ID:SGTC_3207|Compound:9114163|FD-Score:4.18|P-value:4.87E-5 ID:SGTC_3304|Compound:9125678|FD-Score:3.37|P-value:8.50E-4

Top fitness defect scores for YER148W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3202 9113761 7.69 49.5 µM 3.24E-13 1 7.47E-15 1.83
2 SGTC_525 k781-2675 6.60 26.7 µM 3.52E-10 redox potentiating 14 2.11E-11 0.04
3 SGTC_2986 9022445 6.19 71.4 µM 3.62E-9 1 2.99E-10 1.43
4 SGTC_1416 3966-0321 5.59 25.3 µM 8.99E-8 2 1.15E-8 1.05
5 SGTC_1959 st077091 5.46 55.8 µM 1.68E-7 RPP1 & pyrimidine depletion 16 2.34E-8 0.41
6 SGTC_2506 ethyl hematommate 5.45 54.2 µM 1.78E-7 RPP1 & pyrimidine depletion 1 2.50E-8 0.69
7 SGTC_576 1073-0113 5.38 22.7 µM 2.57E-7 redox potentiating 19 3.78E-8 0.07
8 SGTC_1251 0490-5699 5.35 265.0 µM 2.93E-7 2 4.39E-8 0.44
9 SGTC_2810 7959871 5.32 45.5 µM 3.40E-7 copper-dependent oxidative stress 10 5.20E-8 0.16
10 SGTC_1245 0418-0076 5.17 17.2 µM 7.00E-7 ERG2 5 1.18E-7 0.31
11 SGTC_197 4544-0043 4.92 41.6 µM 2.19E-6 endomembrane recycling 16 4.32E-7 0.15
12 SGTC_1415 3966-0071 4.91 78.1 µM 2.29E-6 DNA intercalators 3 4.55E-7 0.46
13 SGTC_1340 1486-1359 4.85 257.0 µM 2.99E-6 2 6.15E-7 0.40
14 SGTC_1623 st003709 4.75 20.3 µM 4.72E-6 calcium & mitochondrial duress 4 1.03E-6 0.04
15 SGTC_1462 k081-0014 4.52 26.5 µM 1.23E-5 1 3.05E-6 0.78
16 SGTC_1219 1469-0211 4.30 33.1 µM 3.03E-5 3 8.52E-6 0.31
17 SGTC_1923 st058458 4.27 19.1 µM 3.40E-5 10 9.70E-6 0.11
18 SGTC_1324 1418-0026 4.27 34.4 µM 3.45E-5 3 9.89E-6 0.01
19 SGTC_3207 9114163 4.18 49.5 µM 4.87E-5 8 1.46E-5 0.05
20 SGTC_1754 st042065 4.08 16.1 µM 7.07E-5 NEO1-PIK1 30 2.23E-5 0.09

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 7.71E-12 YKL058W TOA2 het TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
0.109 2.23E-10 YBL068W PRS4 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication
0.108 3.10E-10 YKL017C HCS1 hom Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities
0.103 2.43E-9 YOR005C DNL4 hom DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth
0.102 2.80E-9 YBR138C_p YBR138C_p hom Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
0.095 3.19E-8 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.095 3.16E-8 YNL082W PMS1 hom ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL
0.094 4.73E-8 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.092 9.00E-8 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.091 1.19E-7 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.091 1.38E-7 YKR083C DAD2 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.091 1.47E-7 YHR188C GPI16 het Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
0.090 1.79E-7 YFL044C OTU1 hom Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress
0.089 2.37E-7 YKL153W_d YKL153W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
0.089 2.50E-7 YHL027W RIM101 hom Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC