YER149C / PEA2

Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth

Zygosity: Homozygous strain
fixedexpanded
Profile for YER149C / PEA2

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:3.10|P-value:8.51E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:4.20|P-value:1.24E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:5.06|P-value:2.02E-7 ID:SGTC_206|Compound:4546-0025|FD-Score:3.70|P-value:9.66E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.15|P-value:7.35E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.25|P-value:5.24E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.22|P-value:9.87E-4 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:-3.33|P-value:6.72E-4 ID:SGTC_565|Compound:1418-0001|FD-Score:3.88|P-value:4.69E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:3.45|P-value:2.54E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:4.45|P-value:3.98E-6 ID:SGTC_767|Compound:0929-0023|FD-Score:3.39|P-value:3.10E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:4.39|P-value:5.27E-6 ID:SGTC_913|Compound:3487-1245|FD-Score:3.19|P-value:6.41E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:3.51|P-value:2.03E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.63|P-value:1.28E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.15|P-value:7.28E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:10.80|P-value:4.73E-27 ID:SGTC_1249|Compound:0481-0015|FD-Score:-4.44|P-value:8.77E-6 ID:SGTC_1252|Compound:0493-0875|FD-Score:11.10|P-value:2.54E-28 ID:SGTC_1621|Compound:st003705|FD-Score:3.29|P-value:4.43E-4 ID:SGTC_1799|Compound:st045822|FD-Score:3.22|P-value:5.68E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-4.07|P-value:4.26E-5 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.84|P-value:5.52E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-5.11|P-value:3.64E-7 ID:SGTC_2246|Compound:7293507|FD-Score:3.24|P-value:5.39E-4 ID:SGTC_2320|Compound:7373003|FD-Score:3.12|P-value:8.22E-4 ID:SGTC_2525|Compound:harmine|FD-Score:3.53|P-value:1.91E-4 ID:SGTC_2814|Compound:7977482|FD-Score:-3.22|P-value:9.90E-4 ID:SGTC_2831|Compound:7999036|FD-Score:3.55|P-value:1.77E-4 ID:SGTC_2850|Compound:9015429|FD-Score:-3.70|P-value:1.81E-4 ID:SGTC_2969|Compound:9091408|FD-Score:-3.70|P-value:1.81E-4 ID:SGTC_3332|Compound:9141069|FD-Score:-3.49|P-value:3.84E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.64|P-value:1.25E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.10|P-value:8.51E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:4.20|P-value:1.24E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:5.06|P-value:2.02E-7 ID:SGTC_206|Compound:4546-0025|FD-Score:3.70|P-value:9.66E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.15|P-value:7.35E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.25|P-value:5.24E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.22|P-value:9.87E-4 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:-3.33|P-value:6.72E-4 ID:SGTC_565|Compound:1418-0001|FD-Score:3.88|P-value:4.69E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:3.45|P-value:2.54E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:4.45|P-value:3.98E-6 ID:SGTC_767|Compound:0929-0023|FD-Score:3.39|P-value:3.10E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:4.39|P-value:5.27E-6 ID:SGTC_913|Compound:3487-1245|FD-Score:3.19|P-value:6.41E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:3.51|P-value:2.03E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.63|P-value:1.28E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.15|P-value:7.28E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:10.80|P-value:4.73E-27 ID:SGTC_1249|Compound:0481-0015|FD-Score:-4.44|P-value:8.77E-6 ID:SGTC_1252|Compound:0493-0875|FD-Score:11.10|P-value:2.54E-28 ID:SGTC_1621|Compound:st003705|FD-Score:3.29|P-value:4.43E-4 ID:SGTC_1799|Compound:st045822|FD-Score:3.22|P-value:5.68E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-4.07|P-value:4.26E-5 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.84|P-value:5.52E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-5.11|P-value:3.64E-7 ID:SGTC_2246|Compound:7293507|FD-Score:3.24|P-value:5.39E-4 ID:SGTC_2320|Compound:7373003|FD-Score:3.12|P-value:8.22E-4 ID:SGTC_2525|Compound:harmine|FD-Score:3.53|P-value:1.91E-4 ID:SGTC_2814|Compound:7977482|FD-Score:-3.22|P-value:9.90E-4 ID:SGTC_2831|Compound:7999036|FD-Score:3.55|P-value:1.77E-4 ID:SGTC_2850|Compound:9015429|FD-Score:-3.70|P-value:1.81E-4 ID:SGTC_2969|Compound:9091408|FD-Score:-3.70|P-value:1.81E-4 ID:SGTC_3332|Compound:9141069|FD-Score:-3.49|P-value:3.84E-4 ID:SGTC_3354|Compound:9151612|FD-Score:3.64|P-value:1.25E-4

Top fitness defect scores for YER149C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1252 0493-0875 11.10 34.8 µM 2.54E-28 1 1.03E-28
2 SGTC_1234 0326-0494 10.80 15.5 µM 4.73E-27 1 2.05E-27
3 SGTC_138 0850-0259 5.06 40.9 µM 2.02E-7 cell wall 142 2.07E-7
4 SGTC_699 0375-0592 4.45 111.0 µM 3.98E-6 iron homeostasis 11 4.24E-6
5 SGTC_873 0591-4978 4.39 488.0 µM 5.27E-6 11 5.63E-6
6 SGTC_135 0933-0004 4.20 26.4 µM 1.24E-5 60S ribosome export 17 1.34E-5
7 SGTC_565 1418-0001 3.88 44.5 µM 4.69E-5 20 5.13E-5
8 SGTC_2130 NSC 7 3.84 2.5 µM 5.52E-5 copper-dependent oxidative stress 29 6.05E-5
9 SGTC_206 4546-0025 3.70 72.0 µM 9.66E-5 NEO1 84 1.06E-4
10 SGTC_3354 9151612 3.64 71.4 µM 1.25E-4 19 1.38E-4
11 SGTC_1129 3453-1384 3.63 223.0 µM 1.28E-4 51 1.41E-4
12 SGTC_2831 7999036 3.55 19.5 µM 1.77E-4 59 1.96E-4
13 SGTC_2525 harmine 3.53 47.9 µM 1.91E-4 ergosterol biosynthesis 17 2.12E-4
14 SGTC_935 2996-0153 3.51 430.0 µM 2.03E-4 PDR1 31 2.24E-4
15 SGTC_577 r071-0003 3.45 38.6 µM 2.54E-4 fatty acid desaturase (OLE1) 82 2.82E-4
16 SGTC_767 0929-0023 3.39 86.0 µM 3.10E-4 TRP & mitochondrial translation 114 3.44E-4
17 SGTC_1621 st003705 3.29 32.4 µM 4.43E-4 calcium & mitochondrial duress 42 4.93E-4
18 SGTC_312 4092-0653 3.25 6.8 µM 5.24E-4 ergosterol depletion effects on membrane 121 5.85E-4
19 SGTC_2246 7293507 3.24 91.6 µM 5.39E-4 60 6.01E-4
20 SGTC_1799 st045822 3.22 46.1 µM 5.68E-4 34 6.34E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.145 3.83E-17 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.142 1.22E-16 YML057C-A_d YML057C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W
0.139 5.21E-16 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.132 1.77E-14 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.124 6.53E-13 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.123 1.03E-12 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.122 1.13E-12 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.121 2.12E-12 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.120 2.89E-12 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.119 4.05E-12 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.117 1.10E-11 YLL021W SPA2 hom Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.115 2.41E-11 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.115 2.81E-11 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.110 1.47E-10 YMR182W-A_p YMR182W-A_p hom Putative protein of unknown function
0.109 2.24E-10 YPR033C HTS1 het Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome