YER159C / BUR6

Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha

Zygosity: Heterozygous strain
fixedexpanded
Profile for YER159C / BUR6

Click on Significant Values for Screen Details ID:SGTC_127|Compound:0180-0333|FD-Score:-3.14|P-value:1.72E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.95|P-value:1.46E-15 ID:SGTC_161|Compound:4065-0411|FD-Score:2.76|P-value:9.27E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.72|P-value:1.31E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:2.89|P-value:5.36E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.05|P-value:2.05E-6 ID:SGTC_420|Compound:mitomycin C|FD-Score:-3.18|P-value:1.46E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:2.88|P-value:5.72E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.02|P-value:3.25E-4 ID:SGTC_443|Compound:clozapine|FD-Score:3.19|P-value:1.53E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.47|P-value:4.42E-5 ID:SGTC_557|Compound:1315-0110|FD-Score:-3.37|P-value:6.26E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:-2.93|P-value:4.28E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.02|P-value:3.19E-4 ID:SGTC_720|Compound:r052-1644|FD-Score:3.81|P-value:8.26E-6 ID:SGTC_737|Compound:1950-0117|FD-Score:3.46|P-value:4.59E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.40|P-value:5.39E-5 ID:SGTC_870|Compound:0573-2213|FD-Score:-3.18|P-value:1.49E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:-2.75|P-value:8.68E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.11|P-value:2.04E-4 ID:SGTC_1519|Compound:esterone|FD-Score:3.42|P-value:5.58E-5 ID:SGTC_1602|Compound:st001878|FD-Score:2.76|P-value:8.94E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.57|P-value:2.68E-5 ID:SGTC_1710|Compound:st031758|FD-Score:3.27|P-value:1.08E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:5.63|P-value:9.29E-11 ID:SGTC_1720|Compound:st037298|FD-Score:3.80|P-value:8.80E-6 ID:SGTC_1915|Compound:st067105|FD-Score:-2.96|P-value:3.71E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.37|P-value:7.03E-5 ID:SGTC_1975|Compound:st070967|FD-Score:3.09|P-value:2.36E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-5.82|P-value:1.93E-11 ID:SGTC_2158|Compound:5657123|FD-Score:2.82|P-value:7.07E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-7.35|P-value:3.51E-17 ID:SGTC_2208|Compound:7283366|FD-Score:2.88|P-value:5.65E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.11|P-value:2.00E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.55|P-value:2.92E-5 ID:SGTC_2296|Compound:7971656|FD-Score:-5.14|P-value:2.54E-9 ID:SGTC_2299|Compound:7976368|FD-Score:3.91|P-value:4.84E-6 ID:SGTC_2337|Compound:9009472|FD-Score:3.00|P-value:3.53E-4 ID:SGTC_2381|Compound:9035592|FD-Score:3.31|P-value:9.13E-5 ID:SGTC_2409|Compound:alverine citrate|FD-Score:5.90|P-value:1.22E-11 ID:SGTC_2433|Compound:5607874|FD-Score:-3.64|P-value:1.74E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.48|P-value:4.07E-5 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.21|P-value:1.31E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:6.02|P-value:4.74E-12 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.04|P-value:2.67E-4 ID:SGTC_2624|Compound:nerol|FD-Score:3.58|P-value:2.55E-5 ID:SGTC_2667|Compound:digitoxin|FD-Score:2.81|P-value:7.52E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.49|P-value:3.54E-5 ID:SGTC_2817|Compound:7995342|FD-Score:-3.04|P-value:2.67E-4 ID:SGTC_2819|Compound:7996655|FD-Score:3.11|P-value:2.15E-4 ID:SGTC_2823|Compound:7987624|FD-Score:-4.58|P-value:9.37E-8 ID:SGTC_2854|Compound:9024559|FD-Score:2.78|P-value:8.34E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-2.82|P-value:6.56E-4 ID:SGTC_2958|Compound:9077254|FD-Score:-3.33|P-value:7.49E-5 ID:SGTC_2964|Compound:9083345|FD-Score:-5.31|P-value:8.07E-10 ID:SGTC_3048|Compound:9093155|FD-Score:3.40|P-value:6.13E-5 ID:SGTC_3049|Compound:9092223|FD-Score:2.84|P-value:6.55E-4 ID:SGTC_3083|Compound:9117465|FD-Score:-2.78|P-value:7.83E-4 ID:SGTC_3089|Compound:9117425|FD-Score:2.80|P-value:7.66E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.14|P-value:1.72E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.95|P-value:1.46E-15 ID:SGTC_161|Compound:4065-0411|FD-Score:2.76|P-value:9.27E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.72|P-value:1.31E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:2.89|P-value:5.36E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.05|P-value:2.05E-6 ID:SGTC_420|Compound:mitomycin C|FD-Score:-3.18|P-value:1.46E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:2.88|P-value:5.72E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.02|P-value:3.25E-4 ID:SGTC_443|Compound:clozapine|FD-Score:3.19|P-value:1.53E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.47|P-value:4.42E-5 ID:SGTC_557|Compound:1315-0110|FD-Score:-3.37|P-value:6.26E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:-2.93|P-value:4.28E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.02|P-value:3.19E-4 ID:SGTC_720|Compound:r052-1644|FD-Score:3.81|P-value:8.26E-6 ID:SGTC_737|Compound:1950-0117|FD-Score:3.46|P-value:4.59E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.40|P-value:5.39E-5 ID:SGTC_870|Compound:0573-2213|FD-Score:-3.18|P-value:1.49E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:-2.75|P-value:8.68E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.11|P-value:2.04E-4 ID:SGTC_1519|Compound:esterone|FD-Score:3.42|P-value:5.58E-5 ID:SGTC_1602|Compound:st001878|FD-Score:2.76|P-value:8.94E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.57|P-value:2.68E-5 ID:SGTC_1710|Compound:st031758|FD-Score:3.27|P-value:1.08E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:5.63|P-value:9.29E-11 ID:SGTC_1720|Compound:st037298|FD-Score:3.80|P-value:8.80E-6 ID:SGTC_1915|Compound:st067105|FD-Score:-2.96|P-value:3.71E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.37|P-value:7.03E-5 ID:SGTC_1975|Compound:st070967|FD-Score:3.09|P-value:2.36E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-5.82|P-value:1.93E-11 ID:SGTC_2158|Compound:5657123|FD-Score:2.82|P-value:7.07E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-7.35|P-value:3.51E-17 ID:SGTC_2208|Compound:7283366|FD-Score:2.88|P-value:5.65E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-3.11|P-value:2.00E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.55|P-value:2.92E-5 ID:SGTC_2296|Compound:7971656|FD-Score:-5.14|P-value:2.54E-9 ID:SGTC_2299|Compound:7976368|FD-Score:3.91|P-value:4.84E-6 ID:SGTC_2337|Compound:9009472|FD-Score:3.00|P-value:3.53E-4 ID:SGTC_2381|Compound:9035592|FD-Score:3.31|P-value:9.13E-5 ID:SGTC_2409|Compound:alverine citrate|FD-Score:5.90|P-value:1.22E-11 ID:SGTC_2433|Compound:5607874|FD-Score:-3.64|P-value:1.74E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.48|P-value:4.07E-5 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.21|P-value:1.31E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:6.02|P-value:4.74E-12 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.04|P-value:2.67E-4 ID:SGTC_2624|Compound:nerol|FD-Score:3.58|P-value:2.55E-5 ID:SGTC_2667|Compound:digitoxin|FD-Score:2.81|P-value:7.52E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.49|P-value:3.54E-5 ID:SGTC_2817|Compound:7995342|FD-Score:-3.04|P-value:2.67E-4 ID:SGTC_2819|Compound:7996655|FD-Score:3.11|P-value:2.15E-4 ID:SGTC_2823|Compound:7987624|FD-Score:-4.58|P-value:9.37E-8 ID:SGTC_2854|Compound:9024559|FD-Score:2.78|P-value:8.34E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-2.82|P-value:6.56E-4 ID:SGTC_2958|Compound:9077254|FD-Score:-3.33|P-value:7.49E-5 ID:SGTC_2964|Compound:9083345|FD-Score:-5.31|P-value:8.07E-10 ID:SGTC_3048|Compound:9093155|FD-Score:3.40|P-value:6.13E-5 ID:SGTC_3049|Compound:9092223|FD-Score:2.84|P-value:6.55E-4 ID:SGTC_3083|Compound:9117465|FD-Score:-2.78|P-value:7.83E-4 ID:SGTC_3089|Compound:9117425|FD-Score:2.80|P-value:7.66E-4

Top fitness defect scores for YER159C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2563 tomatine 6.02 8.5 µM 4.74E-12 4 8.81E-10 0.25
2 SGTC_2409 alverine citrate 5.90 64.0 µM 1.22E-11 6 1.85E-9 0.13
3 SGTC_1718 roemerine 5.63 11.9 µM 9.29E-11 redox potentiating 9 9.06E-9 0.10
4 SGTC_2299 7976368 3.91 114.9 µM 4.84E-6 7 4.59E-5 0.16
5 SGTC_720 r052-1644 3.81 49.9 µM 8.26E-6 TSC3-RPN4 9 6.99E-5 0.03
6 SGTC_1720 st037298 3.80 9.5 µM 8.80E-6 11 7.35E-5 0.36
7 SGTC_198 3454-2873 3.72 16.2 µM 1.31E-5 60S ribosome export 5 1.01E-4 0.07
8 SGTC_2624 nerol 3.58 100.0 µM 2.55E-5 4 1.70E-4 0.02
9 SGTC_1679 4130-1276 3.57 6.7 µM 2.68E-5 Golgi 31 1.77E-4 0.01
10 SGTC_2254 7938975 3.55 95.2 µM 2.92E-5 12 1.90E-4 0.05
11 SGTC_2499 avocadenofuran 3.48 51.5 µM 4.07E-5 6 2.46E-4 0.18
12 SGTC_535 2293-4129 3.47 48.2 µM 4.42E-5 NEO1 15 2.63E-4 0.08
13 SGTC_737 1950-0117 3.46 57.9 µM 4.59E-5 6 2.71E-4 0.02
14 SGTC_1519 esterone 3.42 74.0 µM 5.58E-5 DNA intercalators 9 3.16E-4 0.09
15 SGTC_3048 9093155 3.40 49.5 µM 6.13E-5 3 3.40E-4 0.25
16 SGTC_1962 st076595 3.37 64.5 µM 7.03E-5 redox potentiating 22 3.79E-4 0.00
17 SGTC_2381 9035592 3.31 200.0 µM 9.13E-5 11 4.67E-4 0.15
18 SGTC_1710 st031758 3.27 61.5 µM 1.08E-4 8 5.31E-4 0.11
19 SGTC_443 clozapine 3.19 122.0 µM 1.53E-4 NEO1-PIK1 9 7.01E-4 0.03
20 SGTC_2819 7996655 3.11 46.4 µM 2.15E-4 7 9.20E-4 0.10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.170 3.49E-23 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.165 7.55E-22 YKL122C SRP21 het Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
0.164 1.07E-21 YOR304W ISW2 hom ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth
0.147 1.25E-17 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.144 5.09E-17 YIL046W MET30 het F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
0.139 5.05E-16 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.131 2.91E-14 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.128 1.20E-13 YBL018C POP8 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.127 1.37E-13 YEL002C WBP1 het Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
0.127 1.89E-13 YDR201W SPC19 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
0.124 6.78E-13 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.123 6.93E-13 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.123 1.05E-12 YGR140W CBF2 het Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
0.121 2.31E-12 YPR103W PRE2 het Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.119 3.84E-12 YOL034W SMC5 het Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair