YER162C / RAD4

Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins

Zygosity: Homozygous strain
fixedexpanded
Profile for YER162C / RAD4

Click on Significant Values for Screen Details ID:SGTC_74|Compound:000s-0487|FD-Score:3.86|P-value:5.01E-5 ID:SGTC_136|Compound:0097-0015|FD-Score:3.46|P-value:2.39E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:3.31|P-value:4.22E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.34|P-value:3.77E-4 ID:SGTC_251|Compound:melphalan|FD-Score:10.80|P-value:3.54E-28 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:24.30|P-value:8.03E-134 ID:SGTC_317|Compound:4495-0002|FD-Score:3.87|P-value:4.80E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.16|P-value:7.21E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:4.36|P-value:5.49E-6 ID:SGTC_541|Compound:4513-1321|FD-Score:5.08|P-value:1.45E-7 ID:SGTC_580|Compound:1123-0028|FD-Score:3.17|P-value:6.94E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:3.33|P-value:4.00E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:-3.20|P-value:5.96E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:4.98|P-value:2.51E-7 ID:SGTC_766|Compound:1391-0721|FD-Score:4.19|P-value:1.20E-5 ID:SGTC_771|Compound:0250-0039|FD-Score:5.34|P-value:3.50E-8 ID:SGTC_997|Compound:3996-0078|FD-Score:3.33|P-value:3.98E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.06|P-value:9.66E-4 ID:SGTC_1096|Compound:rimcazole|FD-Score:3.52|P-value:1.92E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.32|P-value:4.05E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:5.43|P-value:2.14E-8 ID:SGTC_1332|Compound:1480-0653|FD-Score:3.29|P-value:4.54E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.08|P-value:9.40E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.08|P-value:9.37E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.99|P-value:2.83E-5 ID:SGTC_1730|Compound:st033808|FD-Score:-3.45|P-value:2.35E-4 ID:SGTC_1736|Compound:st036774|FD-Score:5.34|P-value:3.53E-8 ID:SGTC_1820|Compound:st053765|FD-Score:4.63|P-value:1.49E-6 ID:SGTC_1986|Compound:st071812|FD-Score:-3.39|P-value:2.93E-4 ID:SGTC_2050|Compound:5263056|FD-Score:5.34|P-value:3.49E-8 ID:SGTC_2172|Compound:5754775|FD-Score:6.19|P-value:2.07E-10 ID:SGTC_2174|Compound:5790901|FD-Score:3.41|P-value:2.88E-4 ID:SGTC_2178|Compound:5812956|FD-Score:3.98|P-value:2.98E-5 ID:SGTC_2185|Compound:6113692|FD-Score:-3.08|P-value:9.01E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:4.49|P-value:2.94E-6 ID:SGTC_2261|Compound:9010538|FD-Score:4.25|P-value:8.82E-6 ID:SGTC_2320|Compound:7373003|FD-Score:-3.17|P-value:6.63E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.86|P-value:5.00E-5 ID:SGTC_2677|Compound:mitomycin C|FD-Score:3.14|P-value:7.62E-4 ID:SGTC_2718|Compound:lomofungin|FD-Score:-3.22|P-value:5.57E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.21|P-value:5.63E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.16|P-value:6.93E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:3.81|P-value:6.20E-5 ID:SGTC_2803|Compound:7707263|FD-Score:3.45|P-value:2.47E-4 ID:SGTC_2855|Compound:9016525|FD-Score:-3.53|P-value:1.77E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.10|P-value:8.52E-4 ID:SGTC_2932|Compound:9016711|FD-Score:3.82|P-value:5.91E-5 ID:SGTC_2935|Compound:9038016|FD-Score:3.89|P-value:4.40E-5 ID:SGTC_2966|Compound:9084041|FD-Score:-3.25|P-value:4.99E-4 ID:SGTC_3219|Compound:9131112|FD-Score:3.11|P-value:8.71E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:3.86|P-value:5.01E-5 ID:SGTC_136|Compound:0097-0015|FD-Score:3.46|P-value:2.39E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:3.31|P-value:4.22E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:3.34|P-value:3.77E-4 ID:SGTC_251|Compound:melphalan|FD-Score:10.80|P-value:3.54E-28 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:24.30|P-value:8.03E-134 ID:SGTC_317|Compound:4495-0002|FD-Score:3.87|P-value:4.80E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.16|P-value:7.21E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:4.36|P-value:5.49E-6 ID:SGTC_541|Compound:4513-1321|FD-Score:5.08|P-value:1.45E-7 ID:SGTC_580|Compound:1123-0028|FD-Score:3.17|P-value:6.94E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:3.33|P-value:4.00E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:-3.20|P-value:5.96E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:4.98|P-value:2.51E-7 ID:SGTC_766|Compound:1391-0721|FD-Score:4.19|P-value:1.20E-5 ID:SGTC_771|Compound:0250-0039|FD-Score:5.34|P-value:3.50E-8 ID:SGTC_997|Compound:3996-0078|FD-Score:3.33|P-value:3.98E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.06|P-value:9.66E-4 ID:SGTC_1096|Compound:rimcazole|FD-Score:3.52|P-value:1.92E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.32|P-value:4.05E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:5.43|P-value:2.14E-8 ID:SGTC_1332|Compound:1480-0653|FD-Score:3.29|P-value:4.54E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.08|P-value:9.40E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.08|P-value:9.37E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.99|P-value:2.83E-5 ID:SGTC_1730|Compound:st033808|FD-Score:-3.45|P-value:2.35E-4 ID:SGTC_1736|Compound:st036774|FD-Score:5.34|P-value:3.53E-8 ID:SGTC_1820|Compound:st053765|FD-Score:4.63|P-value:1.49E-6 ID:SGTC_1986|Compound:st071812|FD-Score:-3.39|P-value:2.93E-4 ID:SGTC_2050|Compound:5263056|FD-Score:5.34|P-value:3.49E-8 ID:SGTC_2172|Compound:5754775|FD-Score:6.19|P-value:2.07E-10 ID:SGTC_2174|Compound:5790901|FD-Score:3.41|P-value:2.88E-4 ID:SGTC_2178|Compound:5812956|FD-Score:3.98|P-value:2.98E-5 ID:SGTC_2185|Compound:6113692|FD-Score:-3.08|P-value:9.01E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:4.49|P-value:2.94E-6 ID:SGTC_2261|Compound:9010538|FD-Score:4.25|P-value:8.82E-6 ID:SGTC_2320|Compound:7373003|FD-Score:-3.17|P-value:6.63E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.86|P-value:5.00E-5 ID:SGTC_2677|Compound:mitomycin C|FD-Score:3.14|P-value:7.62E-4 ID:SGTC_2718|Compound:lomofungin|FD-Score:-3.22|P-value:5.57E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.21|P-value:5.63E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.16|P-value:6.93E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:3.81|P-value:6.20E-5 ID:SGTC_2803|Compound:7707263|FD-Score:3.45|P-value:2.47E-4 ID:SGTC_2855|Compound:9016525|FD-Score:-3.53|P-value:1.77E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.10|P-value:8.52E-4 ID:SGTC_2932|Compound:9016711|FD-Score:3.82|P-value:5.91E-5 ID:SGTC_2935|Compound:9038016|FD-Score:3.89|P-value:4.40E-5 ID:SGTC_2966|Compound:9084041|FD-Score:-3.25|P-value:4.99E-4 ID:SGTC_3219|Compound:9131112|FD-Score:3.11|P-value:8.71E-4

Top fitness defect scores for YER162C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 24.30 44.4 nM 8.03E-134 DNA damage response 5 4.11E-131
2 SGTC_251 melphalan 10.80 456.5 µM 3.54E-28 DNA damage response 1 1.17E-27
3 SGTC_2172 5754775 6.19 163.9 µM 2.07E-10 2 3.01E-10
4 SGTC_1243 0416-0003 5.43 5.7 µM 2.14E-8 PDR1 9 2.84E-8
5 SGTC_2050 5263056 5.34 171.0 µM 3.49E-8 fatty acid desaturase (OLE1) 19 4.60E-8
6 SGTC_771 0250-0039 5.34 27.3 µM 3.50E-8 6 4.61E-8
7 SGTC_1736 st036774 5.34 51.0 µM 3.53E-8 11 4.65E-8
8 SGTC_541 4513-1321 5.08 94.0 µM 1.45E-7 8 1.86E-7
9 SGTC_738 1315-0003 4.98 72.4 µM 2.51E-7 9 3.18E-7
10 SGTC_1820 st053765 4.63 18.6 µM 1.49E-6 RPP1 & pyrimidine depletion 4 1.83E-6
11 SGTC_2209 5-(5-methyl-2-thenylidene)-Barbituric acid 4.49 122.8 µM 2.94E-6 2 3.56E-6
12 SGTC_496 calpeptin 4.36 138.0 µM 5.49E-6 10 6.57E-6
13 SGTC_2261 9010538 4.25 184.7 µM 8.82E-6 13 1.05E-5
14 SGTC_766 1391-0721 4.19 126.0 µM 1.20E-5 11 1.42E-5
15 SGTC_1726 st035556 3.99 14.6 µM 2.83E-5 34 3.29E-5
16 SGTC_2178 5812956 3.98 200.0 µM 2.98E-5 18 3.46E-5
17 SGTC_2935 9038016 3.89 30.0 µM 4.40E-5 22 5.06E-5
18 SGTC_317 4495-0002 3.87 170.0 µM 4.80E-5 11 5.52E-5
19 SGTC_2336 9001819 3.86 198.7 µM 5.00E-5 heme biosynthesis & mitochondrial translocase 59 5.74E-5
20 SGTC_74 000s-0487 3.86 160.3 µM 5.01E-5 23 5.76E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.587 2.34E-309 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.553 6.32E-268 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.542 3.29E-255 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.466 8.85E-181 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.350 2.38E-97 YEL037C RAD23 hom Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover
0.329 1.04E-85 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.284 4.28E-63 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.263 2.53E-54 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.210 1.07E-34 YCR066W RAD18 hom E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
0.192 2.92E-29 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.184 4.30E-27 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.184 4.70E-27 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.161 5.65E-21 YIR002C MPH1 hom 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases
0.144 4.29E-17 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.143 1.05E-16 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p